OTP70 is a pentatricopeptide repeat protein of the E subgroup involved in splicing of the plastid transcript rpoC1
Over 20 proteins of the pentatricopeptide repeat (PPR) family have been demonstrated to be involved in RNA editing in plant mitochondria and chloroplasts. All of these editing factors contain a so-called ‘E' domain that has been shown to be essential for editing to occur. The presumption has be...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2011-02, Vol.65 (4), p.532-542 |
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| container_title | The Plant journal : for cell and molecular biology |
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| creator | Chateigner-Boutin, Anne-Laure des Francs-Small, Catherine Colas Delannoy, Etienne Kahlau, Sabine Tanz, Sandra K de Longevialle, Andéol Falcon Fujii, Sota Small, Ian |
| description | Over 20 proteins of the pentatricopeptide repeat (PPR) family have been demonstrated to be involved in RNA editing in plant mitochondria and chloroplasts. All of these editing factors contain a so-called ‘E' domain that has been shown to be essential for editing to occur. The presumption has been that this domain recruits the (unknown) editing enzyme to the RNA. In this report, we show that not all putative E-class PPR proteins are directly involved in RNA editing. Disruption of the OTP70 gene leads to a strong defect in splicing of the plastid transcript rpoC1, leading to a virescent phenotype. The mutant has a chloroplast transcript pattern characteristic of a reduction in plastid-encoded RNA polymerase activity. The E domain of OTP70 is not required for splicing, and can be deleted or replaced by the E domain from the known editing factor CRR4 without loss of rpoC1 splicing. Furthermore, the E domain of OTP70 is incapable of inducing RNA editing when fused to the RNA binding domain of CRR4. We conclude that the truncated E domain of OTP70 is no longer functional in RNA editing, and that the protein has acquired a new function in promoting RNA splicing. |
| doi_str_mv | 10.1111/j.1365-313x.2010.04441.x |
| format | Article |
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All of these editing factors contain a so-called ‘E' domain that has been shown to be essential for editing to occur. The presumption has been that this domain recruits the (unknown) editing enzyme to the RNA. In this report, we show that not all putative E-class PPR proteins are directly involved in RNA editing. Disruption of the OTP70 gene leads to a strong defect in splicing of the plastid transcript rpoC1, leading to a virescent phenotype. The mutant has a chloroplast transcript pattern characteristic of a reduction in plastid-encoded RNA polymerase activity. The E domain of OTP70 is not required for splicing, and can be deleted or replaced by the E domain from the known editing factor CRR4 without loss of rpoC1 splicing. Furthermore, the E domain of OTP70 is incapable of inducing RNA editing when fused to the RNA binding domain of CRR4. We conclude that the truncated E domain of OTP70 is no longer functional in RNA editing, and that the protein has acquired a new function in promoting RNA splicing.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313x.2010.04441.x</identifier><identifier>PMID: 21288264</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; chloroplast ; Gene Expression Regulation, Plant ; Life Sciences ; Plastids - genetics ; PPR protein ; RNA Editing ; RNA polymerase ; RNA Splicing ; RNA, Plant - metabolism ; Vegetal Biology</subject><ispartof>The Plant journal : for cell and molecular biology, 2011-02, Vol.65 (4), p.532-542</ispartof><rights>2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd</rights><rights>2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5421-4da34bd785a79fe782bc30c7a9298a55b9b15d6d0154f1e083e59b5d8237f0113</citedby><cites>FETCH-LOGICAL-c5421-4da34bd785a79fe782bc30c7a9298a55b9b15d6d0154f1e083e59b5d8237f0113</cites><orcidid>0000-0001-5300-1216 ; 0000-0001-9255-1245 ; 0000-0002-0866-2063</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2010.04441.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2010.04441.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,781,785,886,1418,1434,27929,27930,45579,45580,46414,46838</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21288264$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02645122$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Chateigner-Boutin, Anne-Laure</creatorcontrib><creatorcontrib>des Francs-Small, Catherine Colas</creatorcontrib><creatorcontrib>Delannoy, Etienne</creatorcontrib><creatorcontrib>Kahlau, Sabine</creatorcontrib><creatorcontrib>Tanz, Sandra K</creatorcontrib><creatorcontrib>de Longevialle, Andéol Falcon</creatorcontrib><creatorcontrib>Fujii, Sota</creatorcontrib><creatorcontrib>Small, Ian</creatorcontrib><title>OTP70 is a pentatricopeptide repeat protein of the E subgroup involved in splicing of the plastid transcript rpoC1</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Over 20 proteins of the pentatricopeptide repeat (PPR) family have been demonstrated to be involved in RNA editing in plant mitochondria and chloroplasts. All of these editing factors contain a so-called ‘E' domain that has been shown to be essential for editing to occur. The presumption has been that this domain recruits the (unknown) editing enzyme to the RNA. In this report, we show that not all putative E-class PPR proteins are directly involved in RNA editing. Disruption of the OTP70 gene leads to a strong defect in splicing of the plastid transcript rpoC1, leading to a virescent phenotype. The mutant has a chloroplast transcript pattern characteristic of a reduction in plastid-encoded RNA polymerase activity. The E domain of OTP70 is not required for splicing, and can be deleted or replaced by the E domain from the known editing factor CRR4 without loss of rpoC1 splicing. Furthermore, the E domain of OTP70 is incapable of inducing RNA editing when fused to the RNA binding domain of CRR4. We conclude that the truncated E domain of OTP70 is no longer functional in RNA editing, and that the protein has acquired a new function in promoting RNA splicing.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>chloroplast</subject><subject>Gene Expression Regulation, Plant</subject><subject>Life Sciences</subject><subject>Plastids - genetics</subject><subject>PPR protein</subject><subject>RNA Editing</subject><subject>RNA polymerase</subject><subject>RNA Splicing</subject><subject>RNA, Plant - metabolism</subject><subject>Vegetal Biology</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v2yAYxtG0ac3afYWN27SDM_7acNihitp1U6RWairthrCNUyLHMMBZ-u2L56bnwYFXL7_neQUPABCjJc7r226JackLiulxSVDuIsYYXh7fgMXp4vdbsECyREXFMDkDH2LcIYQrWrL34IxgIgQp2QKE281dhaCNUENvhqRTsI3zxifbGhiMNzpBH1wydoCug-nRwCsYx3ob3OihHQ6uP5g2FzD63jZ22J4w3-uYXWAKeohNsD7B4N0KX4B3ne6j-fhynoOH66vN6qZY3_74ubpcFw1nBBes1ZTVbSW4rmRnKkHqhqKm0pJIoTmvZY15W7YIc9ZhgwQ1XNa8FYRWHcKYnoOvs--j7pUPdq_Dk3LaqpvLtZp6KP8Ax4QcJvbLzOan_hlNTGpvY2P6Xg_GjVEJJhkleWdSzGQTXIzBdK_WGKkpHLVTUwZqykBN4ah_4ahjln56GTLWe9O-Ck9pZOD7DPy1vXn6b2O1ufs1VVn_edZ32im9DTaqh_tMUoQllQxJ-gzSuKY8</recordid><startdate>201102</startdate><enddate>201102</enddate><creator>Chateigner-Boutin, Anne-Laure</creator><creator>des Francs-Small, Catherine Colas</creator><creator>Delannoy, Etienne</creator><creator>Kahlau, Sabine</creator><creator>Tanz, Sandra K</creator><creator>de Longevialle, Andéol Falcon</creator><creator>Fujii, Sota</creator><creator>Small, Ian</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-5300-1216</orcidid><orcidid>https://orcid.org/0000-0001-9255-1245</orcidid><orcidid>https://orcid.org/0000-0002-0866-2063</orcidid></search><sort><creationdate>201102</creationdate><title>OTP70 is a pentatricopeptide repeat protein of the E subgroup involved in splicing of the plastid transcript rpoC1</title><author>Chateigner-Boutin, Anne-Laure ; 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All of these editing factors contain a so-called ‘E' domain that has been shown to be essential for editing to occur. The presumption has been that this domain recruits the (unknown) editing enzyme to the RNA. In this report, we show that not all putative E-class PPR proteins are directly involved in RNA editing. Disruption of the OTP70 gene leads to a strong defect in splicing of the plastid transcript rpoC1, leading to a virescent phenotype. The mutant has a chloroplast transcript pattern characteristic of a reduction in plastid-encoded RNA polymerase activity. The E domain of OTP70 is not required for splicing, and can be deleted or replaced by the E domain from the known editing factor CRR4 without loss of rpoC1 splicing. Furthermore, the E domain of OTP70 is incapable of inducing RNA editing when fused to the RNA binding domain of CRR4. 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| source | MEDLINE; Access via Wiley Online Library; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection) |
| subjects | Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism chloroplast Gene Expression Regulation, Plant Life Sciences Plastids - genetics PPR protein RNA Editing RNA polymerase RNA Splicing RNA, Plant - metabolism Vegetal Biology |
| title | OTP70 is a pentatricopeptide repeat protein of the E subgroup involved in splicing of the plastid transcript rpoC1 |
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