The influence of salicylic acid elicitation of shoots, callus, and cell suspension cultures on production of naphtodianthrones and phenylpropanoids in Hypericum perforatum L

Hypericum perforatum is a well known medicinal plant. The main pharmacological properties are due to the presence of naphtodianthrones such as hypericin and pseudohypericin. Unfortunately the levels of these compounds vary under different environmental conditions. Elicitation of in vitro cultures is...

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Veröffentlicht in:Plant cell, tissue and organ culture tissue and organ culture, 2013-04, Vol.113 (1), p.25-39
Hauptverfasser: Gadzovska, Sonia, Maury, Stéphane, Delaunay, Alain, Spasenoski, Mirko, Hagège, Daniel, Courtois, Didier, Joseph, Claude
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container_title Plant cell, tissue and organ culture
container_volume 113
creator Gadzovska, Sonia
Maury, Stéphane
Delaunay, Alain
Spasenoski, Mirko
Hagège, Daniel
Courtois, Didier
Joseph, Claude
description Hypericum perforatum is a well known medicinal plant. The main pharmacological properties are due to the presence of naphtodianthrones such as hypericin and pseudohypericin. Unfortunately the levels of these compounds vary under different environmental conditions. Elicitation of in vitro cultures is a useful approach to enhance and extend production of desirable products. Therefore, the effects of salicylic acid were characterized on different explants of H. perforatum L. (cells, calli and shoots) cultured in vitro. It appears at first that salicylic acid did not affect growth and development of these explants. In addition, the production of both hypericin and pseudohypericin has doubled in elicited cell suspension cultures but not in the two other cultures. Furthermore, phenylpropanoids that are among the most frequently observed metabolites affected upon treatment of in vitro culture material with elicitors, were produced and the enzymatic activities of phenylalanine ammonia lyase and of chalcone isomerase were stimulated upon elicitation. These effects were dependant of the type of in vitro culture, the concentration of salicylic acid and the duration post-elicitation. The H. perforatum cells were globally more sensitive to salicylic acid elicitation when maintained in an undifferentiated state and particularly in cell suspension cultures. In the absence of glands considered as the sites of naphtodianthrones biosynthesis, cells and calli were capable of producing these compounds. This implies that salicylic acid could act at biosynthesis level but not for the accumulation of both hypericin and pseudohypericin. Consequently, the regulation of this process is more complex than cited in the literature involving the responsibility of only Hyp - 1 gene, encoding a hypericin biosynthetic enzyme, cloned and characterized from H. perforatum .
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Furthermore, phenylpropanoids that are among the most frequently observed metabolites affected upon treatment of in vitro culture material with elicitors, were produced and the enzymatic activities of phenylalanine ammonia lyase and of chalcone isomerase were stimulated upon elicitation. These effects were dependant of the type of in vitro culture, the concentration of salicylic acid and the duration post-elicitation. The H. perforatum cells were globally more sensitive to salicylic acid elicitation when maintained in an undifferentiated state and particularly in cell suspension cultures. In the absence of glands considered as the sites of naphtodianthrones biosynthesis, cells and calli were capable of producing these compounds. This implies that salicylic acid could act at biosynthesis level but not for the accumulation of both hypericin and pseudohypericin. 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subjects Acids
Ammonia
Biomedical and Life Sciences
Biosynthesis
Callus
Cell culture
Chalcone isomerase
Environmental conditions
Enzymatic activity
Explants
Glands
Herbal medicine
Hypericin
Hypericum perforatum
Life Sciences
Medicinal plants
Metabolites
Original Paper
Pharmacology
Phenylalanine
Phenylpropanoids
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Salicylic acid
Shoots
title The influence of salicylic acid elicitation of shoots, callus, and cell suspension cultures on production of naphtodianthrones and phenylpropanoids in Hypericum perforatum L
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