Sodium Hydroxide Enhances Extractability and Analysis of Proanthocyanidins in Ensiled Sainfoin (Onobrychis viciifolia)
Little information exists on the effects of ensiling on condensed tannins or proanthocyanidins. The acetone–butanol–HCl assay is suitable for measuring proanthocyanidin contents in a wide range of samples, silages included, but provides limited information on proanthocyanidin composition, which is o...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2015-11, Vol.63 (43), p.9471-9479 |
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| container_issue | 43 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Ramsay, Aina Drake, Chris Grosse Brinkhaus, Anja Girard, Marion Copani, Giuseppe Dohme-Meier, Frigga Bee, Giuseppe Niderkorn, Vincent Mueller-Harvey, Irene |
| description | Little information exists on the effects of ensiling on condensed tannins or proanthocyanidins. The acetone–butanol–HCl assay is suitable for measuring proanthocyanidin contents in a wide range of samples, silages included, but provides limited information on proanthocyanidin composition, which is of interest for deciphering the relationships between tannins and their bioactivities in terms of animal nutrition or health. Degradation with benzyl mercaptan (thiolysis) provides information on proanthocyanidin composition, but proanthocyanidins in several sainfoin silages have proved resistant to thiolysis. We now report that a pretreatment step with sodium hydroxide prior to thiolysis was needed to enable their analysis. This alkaline treatment increased their extractability from ensiled sainfoin and facilitated especially the release of larger proanthocyanidins. Ensiling reduced assayable proanthocyanidins by 29%, but the composition of the remaining proanthocyanidins in silage resembled that of the fresh plants. |
| doi_str_mv | 10.1021/acs.jafc.5b04106 |
| format | Article |
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The acetone–butanol–HCl assay is suitable for measuring proanthocyanidin contents in a wide range of samples, silages included, but provides limited information on proanthocyanidin composition, which is of interest for deciphering the relationships between tannins and their bioactivities in terms of animal nutrition or health. Degradation with benzyl mercaptan (thiolysis) provides information on proanthocyanidin composition, but proanthocyanidins in several sainfoin silages have proved resistant to thiolysis. We now report that a pretreatment step with sodium hydroxide prior to thiolysis was needed to enable their analysis. This alkaline treatment increased their extractability from ensiled sainfoin and facilitated especially the release of larger proanthocyanidins. 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Agric. Food Chem</addtitle><description>Little information exists on the effects of ensiling on condensed tannins or proanthocyanidins. The acetone–butanol–HCl assay is suitable for measuring proanthocyanidin contents in a wide range of samples, silages included, but provides limited information on proanthocyanidin composition, which is of interest for deciphering the relationships between tannins and their bioactivities in terms of animal nutrition or health. Degradation with benzyl mercaptan (thiolysis) provides information on proanthocyanidin composition, but proanthocyanidins in several sainfoin silages have proved resistant to thiolysis. We now report that a pretreatment step with sodium hydroxide prior to thiolysis was needed to enable their analysis. This alkaline treatment increased their extractability from ensiled sainfoin and facilitated especially the release of larger proanthocyanidins. Ensiling reduced assayable proanthocyanidins by 29%, but the composition of the remaining proanthocyanidins in silage resembled that of the fresh plants.</description><subject>Chemical Fractionation - methods</subject><subject>Fabaceae - chemistry</subject><subject>Fabaceae - metabolism</subject><subject>Food and Nutrition</subject><subject>Life Sciences</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - metabolism</subject><subject>Proanthocyanidins - analysis</subject><subject>Proanthocyanidins - isolation & purification</subject><subject>Proanthocyanidins - metabolism</subject><subject>Sodium Hydroxide - chemistry</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFr2zAUh8VYWbO2952Gjy3M2ZNs2dIxlGwZBFrodhbPskRUHKmT7FD_91OWrLedBI_v-x30EfKJwpICo19Rp-UzWr3kHdQUmndkQTmDklMq3pMFZKYUvKGX5GNKzwAgeAsfyCVralFLwRfk8BR6N-2LzdzH8Op6U6z9Dr02qVi_jhH1iJ0b3DgX6Pti5XGYk0tFsMVjDOjHXdAzetc7nwrns5zcYPriCZ23IR9uH3zo4qx3WTo47ZwNg8O7a3JhcUjm5vxekV_f1j_vN-X24fuP-9W2xJqysWxAGKRVr4W1mmsGrUYuGQKzsmGG1UYywK6rWtnpjjNRWQm6lVy00vQSqityd9rd4aBeottjnFVApzarrTregDWVlKI90MzentiXGH5PJo1q75I2w4DehCkp2lbQiKaidUbhhOoYUorGvm1TUMcyKpdRxzLqXCYrn8_rU7c3_ZvwL0UGvpyAv2qYYv7r9P-9P5J2msU</recordid><startdate>20151104</startdate><enddate>20151104</enddate><creator>Ramsay, Aina</creator><creator>Drake, Chris</creator><creator>Grosse Brinkhaus, Anja</creator><creator>Girard, Marion</creator><creator>Copani, Giuseppe</creator><creator>Dohme-Meier, Frigga</creator><creator>Bee, Giuseppe</creator><creator>Niderkorn, Vincent</creator><creator>Mueller-Harvey, Irene</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-6024-4184</orcidid><orcidid>https://orcid.org/0000-0002-4631-7623</orcidid></search><sort><creationdate>20151104</creationdate><title>Sodium Hydroxide Enhances Extractability and Analysis of Proanthocyanidins in Ensiled Sainfoin (Onobrychis viciifolia)</title><author>Ramsay, Aina ; Drake, Chris ; Grosse Brinkhaus, Anja ; Girard, Marion ; Copani, Giuseppe ; Dohme-Meier, Frigga ; Bee, Giuseppe ; Niderkorn, Vincent ; Mueller-Harvey, Irene</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a412t-608ea13dc8ffc5c207ca592a02f962e24e920abb379bcb5283f90c795879ed903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Chemical Fractionation - methods</topic><topic>Fabaceae - chemistry</topic><topic>Fabaceae - metabolism</topic><topic>Food and Nutrition</topic><topic>Life Sciences</topic><topic>Plant Extracts - analysis</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - metabolism</topic><topic>Proanthocyanidins - analysis</topic><topic>Proanthocyanidins - isolation & purification</topic><topic>Proanthocyanidins - metabolism</topic><topic>Sodium Hydroxide - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramsay, Aina</creatorcontrib><creatorcontrib>Drake, Chris</creatorcontrib><creatorcontrib>Grosse Brinkhaus, Anja</creatorcontrib><creatorcontrib>Girard, Marion</creatorcontrib><creatorcontrib>Copani, Giuseppe</creatorcontrib><creatorcontrib>Dohme-Meier, Frigga</creatorcontrib><creatorcontrib>Bee, Giuseppe</creatorcontrib><creatorcontrib>Niderkorn, Vincent</creatorcontrib><creatorcontrib>Mueller-Harvey, Irene</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramsay, Aina</au><au>Drake, Chris</au><au>Grosse Brinkhaus, Anja</au><au>Girard, Marion</au><au>Copani, Giuseppe</au><au>Dohme-Meier, Frigga</au><au>Bee, Giuseppe</au><au>Niderkorn, Vincent</au><au>Mueller-Harvey, Irene</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sodium Hydroxide Enhances Extractability and Analysis of Proanthocyanidins in Ensiled Sainfoin (Onobrychis viciifolia)</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. 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| ispartof | Journal of agricultural and food chemistry, 2015-11, Vol.63 (43), p.9471-9479 |
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| source | American Chemical Society; MEDLINE |
| subjects | Chemical Fractionation - methods Fabaceae - chemistry Fabaceae - metabolism Food and Nutrition Life Sciences Plant Extracts - analysis Plant Extracts - isolation & purification Plant Extracts - metabolism Proanthocyanidins - analysis Proanthocyanidins - isolation & purification Proanthocyanidins - metabolism Sodium Hydroxide - chemistry |
| title | Sodium Hydroxide Enhances Extractability and Analysis of Proanthocyanidins in Ensiled Sainfoin (Onobrychis viciifolia) |
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