Development of a LC-MS/MS method for the simultaneous screening of seven water-soluble vitamins in processing semi-coarse wheat flour products
Wheat is the second largest crop cultivated around the world and constitutes a major part of the daily diet in Europe. It is therefore important to determine the content of micronutrient in wheat and wheat-based food products to define the contribution of wheat-based foods to the nutrition of the co...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2015-05, Vol.407 (12), p.3471-3479 |
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| creator | Nurit, Eric Lyan, Bernard Piquet, Agnès Branlard, Gérard Pujos-Guillot, Estelle |
| description | Wheat is the second largest crop cultivated around the world and constitutes a major part of the daily diet in Europe. It is therefore important to determine the content of micronutrient in wheat and wheat-based food products to define the contribution of wheat-based foods to the nutrition of the consumers. The aim of the present work was to develop a simple and rapid method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of seven water-soluble vitamins in various wheat-based food materials. The vitamins present in the test material were separated in less than 15 min by using a reverse-phase C18 column, and analyzed by positive ion electrospray selected reaction monitoring MS/MS. The MS response for all the vitamins was linear over the working range (0.05 to 9 μg/mL) with correlation coefficients ranging between 0.991 and 1. Limits of quantification in the different food materials ranged from 0.09 to 3.5 μg/g. Intra-day and inter-day precision were found satisfactory. The developed method was applied for the simultaneous analysis of the water-soluble vitamin natural content of different semi-coarse wheat flours and in their corresponding baking products.
Graphical Abstract
Simultaneous screening of 7 water-soluble vitamins in semi-coarse wheat flour, dough, bread and toasted bread |
| doi_str_mv | 10.1007/s00216-015-8553-1 |
| format | Article |
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Graphical Abstract
Simultaneous screening of 7 water-soluble vitamins in semi-coarse wheat flour, dough, bread and toasted bread</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-015-8553-1</identifier><identifier>PMID: 25701425</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acids ; Agricultural sciences ; Analysis ; Analytical Chemistry ; Biochemistry ; Biomaterials ; Biotechnology ; Carbon Isotopes ; Characterization and Evaluation of Materials ; Chemical Sciences ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Chromatography, Reverse-Phase - methods ; Correlation coefficient ; Diets ; Flour ; Flour - analysis ; Food ; Food Analysis - methods ; Food and Nutrition ; Food products ; Food Science ; Foods ; Grain ; Hydrolysis ; Laboratory Medicine ; Life Sciences ; Limit of Detection ; Liquid chromatography ; Mass spectrometry ; Materials selection ; Mathematical analysis ; Methods ; Monitoring ; Monitoring/Environmental Analysis ; Nutrition ; Prostate cancer ; Reproducibility of Results ; Research Paper ; Scientific imaging ; Screening ; Solid Phase Extraction ; Solubility ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry - methods ; Triticum - chemistry ; Vitamin B ; Vitamins ; Vitamins - analysis ; Vitamins - chemistry ; Wheat</subject><ispartof>Analytical and bioanalytical chemistry, 2015-05, Vol.407 (12), p.3471-3479</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>COPYRIGHT 2015 Springer</rights><rights>Copyright</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c646t-1b3886aaf501a0b2eea1e42d7b1eb86f6e63685c7b0abd87f928fbb5bcfb613f3</citedby><cites>FETCH-LOGICAL-c646t-1b3886aaf501a0b2eea1e42d7b1eb86f6e63685c7b0abd87f928fbb5bcfb613f3</cites><orcidid>0009-0008-8777-5533 ; 0000-0002-9148-0956 ; 0000-0002-4693-5712</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-015-8553-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-015-8553-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25701425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02637522$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Nurit, Eric</creatorcontrib><creatorcontrib>Lyan, Bernard</creatorcontrib><creatorcontrib>Piquet, Agnès</creatorcontrib><creatorcontrib>Branlard, Gérard</creatorcontrib><creatorcontrib>Pujos-Guillot, Estelle</creatorcontrib><title>Development of a LC-MS/MS method for the simultaneous screening of seven water-soluble vitamins in processing semi-coarse wheat flour products</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Wheat is the second largest crop cultivated around the world and constitutes a major part of the daily diet in Europe. It is therefore important to determine the content of micronutrient in wheat and wheat-based food products to define the contribution of wheat-based foods to the nutrition of the consumers. The aim of the present work was to develop a simple and rapid method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of seven water-soluble vitamins in various wheat-based food materials. The vitamins present in the test material were separated in less than 15 min by using a reverse-phase C18 column, and analyzed by positive ion electrospray selected reaction monitoring MS/MS. The MS response for all the vitamins was linear over the working range (0.05 to 9 μg/mL) with correlation coefficients ranging between 0.991 and 1. Limits of quantification in the different food materials ranged from 0.09 to 3.5 μg/g. Intra-day and inter-day precision were found satisfactory. The developed method was applied for the simultaneous analysis of the water-soluble vitamin natural content of different semi-coarse wheat flours and in their corresponding baking products.
Graphical Abstract
Simultaneous screening of 7 water-soluble vitamins in semi-coarse wheat flour, dough, bread and toasted bread</description><subject>Acids</subject><subject>Agricultural sciences</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Carbon Isotopes</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical Sciences</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Chromatography, Reverse-Phase - methods</subject><subject>Correlation coefficient</subject><subject>Diets</subject><subject>Flour</subject><subject>Flour - analysis</subject><subject>Food</subject><subject>Food Analysis - methods</subject><subject>Food and Nutrition</subject><subject>Food products</subject><subject>Food Science</subject><subject>Foods</subject><subject>Grain</subject><subject>Hydrolysis</subject><subject>Laboratory Medicine</subject><subject>Life Sciences</subject><subject>Limit of Detection</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Materials selection</subject><subject>Mathematical analysis</subject><subject>Methods</subject><subject>Monitoring</subject><subject>Monitoring/Environmental Analysis</subject><subject>Nutrition</subject><subject>Prostate cancer</subject><subject>Reproducibility of Results</subject><subject>Research Paper</subject><subject>Scientific imaging</subject><subject>Screening</subject><subject>Solid Phase Extraction</subject><subject>Solubility</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tandem Mass Spectrometry - 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methods</topic><topic>Correlation coefficient</topic><topic>Diets</topic><topic>Flour</topic><topic>Flour - analysis</topic><topic>Food</topic><topic>Food Analysis - methods</topic><topic>Food and Nutrition</topic><topic>Food products</topic><topic>Food Science</topic><topic>Foods</topic><topic>Grain</topic><topic>Hydrolysis</topic><topic>Laboratory Medicine</topic><topic>Life Sciences</topic><topic>Limit of Detection</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Materials selection</topic><topic>Mathematical analysis</topic><topic>Methods</topic><topic>Monitoring</topic><topic>Monitoring/Environmental Analysis</topic><topic>Nutrition</topic><topic>Prostate cancer</topic><topic>Reproducibility of Results</topic><topic>Research Paper</topic><topic>Scientific imaging</topic><topic>Screening</topic><topic>Solid Phase Extraction</topic><topic>Solubility</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Triticum - 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It is therefore important to determine the content of micronutrient in wheat and wheat-based food products to define the contribution of wheat-based foods to the nutrition of the consumers. The aim of the present work was to develop a simple and rapid method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of seven water-soluble vitamins in various wheat-based food materials. The vitamins present in the test material were separated in less than 15 min by using a reverse-phase C18 column, and analyzed by positive ion electrospray selected reaction monitoring MS/MS. The MS response for all the vitamins was linear over the working range (0.05 to 9 μg/mL) with correlation coefficients ranging between 0.991 and 1. Limits of quantification in the different food materials ranged from 0.09 to 3.5 μg/g. Intra-day and inter-day precision were found satisfactory. The developed method was applied for the simultaneous analysis of the water-soluble vitamin natural content of different semi-coarse wheat flours and in their corresponding baking products.
Graphical Abstract
Simultaneous screening of 7 water-soluble vitamins in semi-coarse wheat flour, dough, bread and toasted bread</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25701425</pmid><doi>10.1007/s00216-015-8553-1</doi><tpages>9</tpages><orcidid>https://orcid.org/0009-0008-8777-5533</orcidid><orcidid>https://orcid.org/0000-0002-9148-0956</orcidid><orcidid>https://orcid.org/0000-0002-4693-5712</orcidid></addata></record> |
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| ispartof | Analytical and bioanalytical chemistry, 2015-05, Vol.407 (12), p.3471-3479 |
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| subjects | Acids Agricultural sciences Analysis Analytical Chemistry Biochemistry Biomaterials Biotechnology Carbon Isotopes Characterization and Evaluation of Materials Chemical Sciences Chemistry Chemistry and Materials Science Chromatography Chromatography, Reverse-Phase - methods Correlation coefficient Diets Flour Flour - analysis Food Food Analysis - methods Food and Nutrition Food products Food Science Foods Grain Hydrolysis Laboratory Medicine Life Sciences Limit of Detection Liquid chromatography Mass spectrometry Materials selection Mathematical analysis Methods Monitoring Monitoring/Environmental Analysis Nutrition Prostate cancer Reproducibility of Results Research Paper Scientific imaging Screening Solid Phase Extraction Solubility Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry - methods Triticum - chemistry Vitamin B Vitamins Vitamins - analysis Vitamins - chemistry Wheat |
| title | Development of a LC-MS/MS method for the simultaneous screening of seven water-soluble vitamins in processing semi-coarse wheat flour products |
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