Early pregnancy diagnosis in sheep using near-infrared spectroscopy on blood plasma
The objective of this study was to evaluate the ability of near-infrared reflectance spectroscopy (NIRS) to discriminate between pregnant and nonpregnant ewes in early stages of pregnancy after artificial insemination (AI) from blood plasma. Samples were collected using jugular puncture at 18 and 25...
Gespeichert in:
| Veröffentlicht in: | Theriogenology 2014-02, Vol.81 (3), p.509-513 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 513 |
|---|---|
| container_issue | 3 |
| container_start_page | 509 |
| container_title | Theriogenology |
| container_volume | 81 |
| creator | Andueza, Donato Alabart, José L. Lahoz, Belén Muñoz, Fernando Folch, José |
| description | The objective of this study was to evaluate the ability of near-infrared reflectance spectroscopy (NIRS) to discriminate between pregnant and nonpregnant ewes in early stages of pregnancy after artificial insemination (AI) from blood plasma. Samples were collected using jugular puncture at 18 and 25 days after AI from 188 Rasa Aragonesa and Ansotana ewes. Plasma samples were analyzed for pregnancy-associated glycoprotein (PAG) and progesterone (P4) using ELISA commercial kits. The spectra of plasma samples were recorded in the visible and near-infrared ranges. The performance of these tests were compared, using as criterion standard the pregnancy status determined using transabdominal ultrasonography at 45 days after AI. Pregnancy rate was 47.9% (90/188). At Day 18, sensitivity was similar in NIRS and P4 tests (98.9% vs. 100%; not significant) and greater than PAG (32.2%; both P < 0.001). Specificity was similar in NIRS and PAG tests (both 100%) and greater than that of P4 (84.7%; P < 0.001). At Day 25, sensitivity and specificity of NIRS and PAG were both 100%. It can be concluded that NIRS was an accurate method of diagnosis of pregnancy at Days 18 and 25 after AI in ewes. |
| doi_str_mv | 10.1016/j.theriogenology.2013.10.016 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_02631596v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0093691X13004251</els_id><sourcerecordid>1490761592</sourcerecordid><originalsourceid>FETCH-LOGICAL-c486t-529be8676a4bb7b72343dca305ce0cd7e115b0770c41199906b24705ceea6e5a3</originalsourceid><addsrcrecordid>eNqNkUFrGzEQhUVpady0f6Ho0EN7WGck7UoW9BJC0gQMOTSB3oRWO17LrKWtZAf230eL00BvOQ3M-2aGeY-QbwyWDJi82C0PW0w-9hjiEPtpyYGJIi2L-I4s2ErpSnDB3pMFgBaV1OzPGfmU8w4AhJTsIznjNdcglFiQ39c2DRMdE_bBBjfRzts-xOwz9YHmLeJIj9mHnga0qfJhk2zCjuYR3SHF7OI40RhoO8TY0XGweW8_kw8bO2T88lLPyePN9cPVbbW-_3V3dbmuXL2Sh6rhusWVVNLWbataxUUtOmcFNA7BdQoZa1pQClzNmNYaZMtrNatoJTZWnJMfp71bO5gx-b1Nk4nWm9vLtZl7wKVgjZZPrLDfT-yY4t8j5oPZ--xwGGzAeMyG1RqULDAv6M8T6sp_OeHmdTcDM0dgdub_CMwcwawWsYx_fbl0bPfYvQ7_87wANycAizdPHpPJzmNw2PlUTDVd9G-79AxVJqCi</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1490761592</pqid></control><display><type>article</type><title>Early pregnancy diagnosis in sheep using near-infrared spectroscopy on blood plasma</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Andueza, Donato ; Alabart, José L. ; Lahoz, Belén ; Muñoz, Fernando ; Folch, José</creator><creatorcontrib>Andueza, Donato ; Alabart, José L. ; Lahoz, Belén ; Muñoz, Fernando ; Folch, José</creatorcontrib><description>The objective of this study was to evaluate the ability of near-infrared reflectance spectroscopy (NIRS) to discriminate between pregnant and nonpregnant ewes in early stages of pregnancy after artificial insemination (AI) from blood plasma. Samples were collected using jugular puncture at 18 and 25 days after AI from 188 Rasa Aragonesa and Ansotana ewes. Plasma samples were analyzed for pregnancy-associated glycoprotein (PAG) and progesterone (P4) using ELISA commercial kits. The spectra of plasma samples were recorded in the visible and near-infrared ranges. The performance of these tests were compared, using as criterion standard the pregnancy status determined using transabdominal ultrasonography at 45 days after AI. Pregnancy rate was 47.9% (90/188). At Day 18, sensitivity was similar in NIRS and P4 tests (98.9% vs. 100%; not significant) and greater than PAG (32.2%; both P < 0.001). Specificity was similar in NIRS and PAG tests (both 100%) and greater than that of P4 (84.7%; P < 0.001). At Day 25, sensitivity and specificity of NIRS and PAG were both 100%. It can be concluded that NIRS was an accurate method of diagnosis of pregnancy at Days 18 and 25 after AI in ewes.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2013.10.016</identifier><identifier>PMID: 24290373</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Early pregnancy diagnosis ; Ewe ; Female ; Fertility ; Life Sciences ; NIRS ; PAG ; Plasma - chemistry ; Pregnancy ; Pregnancy Tests - methods ; Pregnancy Tests - veterinary ; Pregnancy, Animal - blood ; Progesterone ; Reproductive Biology ; Sensitivity and Specificity ; Sheep - blood ; Spectroscopy, Near-Infrared - veterinary</subject><ispartof>Theriogenology, 2014-02, Vol.81 (3), p.509-513</ispartof><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-529be8676a4bb7b72343dca305ce0cd7e115b0770c41199906b24705ceea6e5a3</citedby><cites>FETCH-LOGICAL-c486t-529be8676a4bb7b72343dca305ce0cd7e115b0770c41199906b24705ceea6e5a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2013.10.016$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24290373$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02631596$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Andueza, Donato</creatorcontrib><creatorcontrib>Alabart, José L.</creatorcontrib><creatorcontrib>Lahoz, Belén</creatorcontrib><creatorcontrib>Muñoz, Fernando</creatorcontrib><creatorcontrib>Folch, José</creatorcontrib><title>Early pregnancy diagnosis in sheep using near-infrared spectroscopy on blood plasma</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>The objective of this study was to evaluate the ability of near-infrared reflectance spectroscopy (NIRS) to discriminate between pregnant and nonpregnant ewes in early stages of pregnancy after artificial insemination (AI) from blood plasma. Samples were collected using jugular puncture at 18 and 25 days after AI from 188 Rasa Aragonesa and Ansotana ewes. Plasma samples were analyzed for pregnancy-associated glycoprotein (PAG) and progesterone (P4) using ELISA commercial kits. The spectra of plasma samples were recorded in the visible and near-infrared ranges. The performance of these tests were compared, using as criterion standard the pregnancy status determined using transabdominal ultrasonography at 45 days after AI. Pregnancy rate was 47.9% (90/188). At Day 18, sensitivity was similar in NIRS and P4 tests (98.9% vs. 100%; not significant) and greater than PAG (32.2%; both P < 0.001). Specificity was similar in NIRS and PAG tests (both 100%) and greater than that of P4 (84.7%; P < 0.001). At Day 25, sensitivity and specificity of NIRS and PAG were both 100%. It can be concluded that NIRS was an accurate method of diagnosis of pregnancy at Days 18 and 25 after AI in ewes.</description><subject>Animals</subject><subject>Early pregnancy diagnosis</subject><subject>Ewe</subject><subject>Female</subject><subject>Fertility</subject><subject>Life Sciences</subject><subject>NIRS</subject><subject>PAG</subject><subject>Plasma - chemistry</subject><subject>Pregnancy</subject><subject>Pregnancy Tests - methods</subject><subject>Pregnancy Tests - veterinary</subject><subject>Pregnancy, Animal - blood</subject><subject>Progesterone</subject><subject>Reproductive Biology</subject><subject>Sensitivity and Specificity</subject><subject>Sheep - blood</subject><subject>Spectroscopy, Near-Infrared - veterinary</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFrGzEQhUVpady0f6Ho0EN7WGck7UoW9BJC0gQMOTSB3oRWO17LrKWtZAf230eL00BvOQ3M-2aGeY-QbwyWDJi82C0PW0w-9hjiEPtpyYGJIi2L-I4s2ErpSnDB3pMFgBaV1OzPGfmU8w4AhJTsIznjNdcglFiQ39c2DRMdE_bBBjfRzts-xOwz9YHmLeJIj9mHnga0qfJhk2zCjuYR3SHF7OI40RhoO8TY0XGweW8_kw8bO2T88lLPyePN9cPVbbW-_3V3dbmuXL2Sh6rhusWVVNLWbataxUUtOmcFNA7BdQoZa1pQClzNmNYaZMtrNatoJTZWnJMfp71bO5gx-b1Nk4nWm9vLtZl7wKVgjZZPrLDfT-yY4t8j5oPZ--xwGGzAeMyG1RqULDAv6M8T6sp_OeHmdTcDM0dgdub_CMwcwawWsYx_fbl0bPfYvQ7_87wANycAizdPHpPJzmNw2PlUTDVd9G-79AxVJqCi</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>Andueza, Donato</creator><creator>Alabart, José L.</creator><creator>Lahoz, Belén</creator><creator>Muñoz, Fernando</creator><creator>Folch, José</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20140201</creationdate><title>Early pregnancy diagnosis in sheep using near-infrared spectroscopy on blood plasma</title><author>Andueza, Donato ; Alabart, José L. ; Lahoz, Belén ; Muñoz, Fernando ; Folch, José</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-529be8676a4bb7b72343dca305ce0cd7e115b0770c41199906b24705ceea6e5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Early pregnancy diagnosis</topic><topic>Ewe</topic><topic>Female</topic><topic>Fertility</topic><topic>Life Sciences</topic><topic>NIRS</topic><topic>PAG</topic><topic>Plasma - chemistry</topic><topic>Pregnancy</topic><topic>Pregnancy Tests - methods</topic><topic>Pregnancy Tests - veterinary</topic><topic>Pregnancy, Animal - blood</topic><topic>Progesterone</topic><topic>Reproductive Biology</topic><topic>Sensitivity and Specificity</topic><topic>Sheep - blood</topic><topic>Spectroscopy, Near-Infrared - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Andueza, Donato</creatorcontrib><creatorcontrib>Alabart, José L.</creatorcontrib><creatorcontrib>Lahoz, Belén</creatorcontrib><creatorcontrib>Muñoz, Fernando</creatorcontrib><creatorcontrib>Folch, José</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Andueza, Donato</au><au>Alabart, José L.</au><au>Lahoz, Belén</au><au>Muñoz, Fernando</au><au>Folch, José</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Early pregnancy diagnosis in sheep using near-infrared spectroscopy on blood plasma</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2014-02-01</date><risdate>2014</risdate><volume>81</volume><issue>3</issue><spage>509</spage><epage>513</epage><pages>509-513</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>The objective of this study was to evaluate the ability of near-infrared reflectance spectroscopy (NIRS) to discriminate between pregnant and nonpregnant ewes in early stages of pregnancy after artificial insemination (AI) from blood plasma. Samples were collected using jugular puncture at 18 and 25 days after AI from 188 Rasa Aragonesa and Ansotana ewes. Plasma samples were analyzed for pregnancy-associated glycoprotein (PAG) and progesterone (P4) using ELISA commercial kits. The spectra of plasma samples were recorded in the visible and near-infrared ranges. The performance of these tests were compared, using as criterion standard the pregnancy status determined using transabdominal ultrasonography at 45 days after AI. Pregnancy rate was 47.9% (90/188). At Day 18, sensitivity was similar in NIRS and P4 tests (98.9% vs. 100%; not significant) and greater than PAG (32.2%; both P < 0.001). Specificity was similar in NIRS and PAG tests (both 100%) and greater than that of P4 (84.7%; P < 0.001). At Day 25, sensitivity and specificity of NIRS and PAG were both 100%. It can be concluded that NIRS was an accurate method of diagnosis of pregnancy at Days 18 and 25 after AI in ewes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>24290373</pmid><doi>10.1016/j.theriogenology.2013.10.016</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0093-691X |
| ispartof | Theriogenology, 2014-02, Vol.81 (3), p.509-513 |
| issn | 0093-691X 1879-3231 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02631596v1 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Early pregnancy diagnosis Ewe Female Fertility Life Sciences NIRS PAG Plasma - chemistry Pregnancy Pregnancy Tests - methods Pregnancy Tests - veterinary Pregnancy, Animal - blood Progesterone Reproductive Biology Sensitivity and Specificity Sheep - blood Spectroscopy, Near-Infrared - veterinary |
| title | Early pregnancy diagnosis in sheep using near-infrared spectroscopy on blood plasma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T15%3A37%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Early%20pregnancy%20diagnosis%20in%20sheep%20using%20near-infrared%20spectroscopy%20on%20blood%20plasma&rft.jtitle=Theriogenology&rft.au=Andueza,%20Donato&rft.date=2014-02-01&rft.volume=81&rft.issue=3&rft.spage=509&rft.epage=513&rft.pages=509-513&rft.issn=0093-691X&rft.eissn=1879-3231&rft_id=info:doi/10.1016/j.theriogenology.2013.10.016&rft_dat=%3Cproquest_hal_p%3E1490761592%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1490761592&rft_id=info:pmid/24290373&rft_els_id=S0093691X13004251&rfr_iscdi=true |