Direct and maternal n-3 long-chain polyunsaturated fatty acid supplementation improved triglyceridemia and glycemia through the regulation of hepatic and muscle sphingolipid synthesis in offspring hamsters fed a high-fat diet
PURPOSE: We recently reported that direct and maternal supplementation with n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) alleviates the metabolic disturbances in adult hamster pups fed with a high-fat diet (HFD). In this study, we hypothesized that these results involved a perinatal modu...
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| Veröffentlicht in: | European journal of nutrition 2016-03, Vol.55 (2), p.589-599 |
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| creator | Kasbi-Chadli, Fatima Ferchaud-Roucher, Véronique Krempf, Michel Ouguerram, Khadija |
| description | PURPOSE: We recently reported that direct and maternal supplementation with n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) alleviates the metabolic disturbances in adult hamster pups fed with a high-fat diet (HFD). In this study, we hypothesized that these results involved a perinatal modulating effect of sphingolipids by n-3 LC-PUFA. METHODS: We studied the effect of direct and maternal n-3 LC-PUFA supplementation on sphingolipid contents in liver and muscle, hepatic triglycerides (TG) secretion and glucose tolerance. Offspring male hamsters born from supplemented (Cω) or unsupplemented (C) mothers were subjected after weaning to a HFD during 16 weeks, without (Cω-HF or C-HF) or with direct supplementation with n-3 LC-PUFA (C-HFω). RESULTS: Direct supplementation decreased sphingosine, sphinganine and ceramides in liver and decreased sphingosine, sphinganine, sphingosine-1-phosphate (S1P) and ceramides in muscle in C-HFω compared to C-HF (p |
| doi_str_mv | 10.1007/s00394-015-0879-0 |
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In this study, we hypothesized that these results involved a perinatal modulating effect of sphingolipids by n-3 LC-PUFA. METHODS: We studied the effect of direct and maternal n-3 LC-PUFA supplementation on sphingolipid contents in liver and muscle, hepatic triglycerides (TG) secretion and glucose tolerance. Offspring male hamsters born from supplemented (Cω) or unsupplemented (C) mothers were subjected after weaning to a HFD during 16 weeks, without (Cω-HF or C-HF) or with direct supplementation with n-3 LC-PUFA (C-HFω). RESULTS: Direct supplementation decreased sphingosine, sphinganine and ceramides in liver and decreased sphingosine, sphinganine, sphingosine-1-phosphate (S1P) and ceramides in muscle in C-HFω compared to C-HF (p < 0.05). Maternal supplementation decreased C20 ceramide and lactosylceramide in liver and sphinganine, S1P and lactosylceramide in muscle (p < 0.05). This supplementation tended to decrease glucosylceramide in liver (p < 0.06) and muscle (p < 0.07) in Cω-HF compared to C-HF. Direct supplementation increased glucose tolerance and decreased hepatic TG secretion and hepatic gene expression levels of diacylglycerol O-acyltransferase 2 (DGAT2), sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase, stearoyl-CoA desaturase-1 (SCD1) and tumor necrosis factor α (TNFα). Maternal supplementation decreased basal glycemia and hepatic TG secretion. We observed a positive correlation between hepatic TG secretion and hepatic ceramide (p = 0.0059), and between basal glycemia and hepatic ceramide (p = 0.04) or muscle lactosylceramide contents (p = 0.001). CONCLUSION: We observed an improvement of lipids and glucose metabolism in hamster with n-3 LC-PUFA direct supplementation and a decrease in glycemia and hepatic TG secretion with maternal supplementation. These results are probably related to a decrease in both lipogenesis and sphingolipid contents in liver and muscle.</description><identifier>ISSN: 1436-6207</identifier><identifier>EISSN: 1436-6215</identifier><identifier>DOI: 10.1007/s00394-015-0879-0</identifier><identifier>PMID: 25787885</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adipose Tissue - drug effects ; Adipose Tissue - metabolism ; adults ; Animals ; Antigens, CD - blood ; blood glucose ; Blood Glucose - metabolism ; ceramides ; Ceramides - metabolism ; Chemistry ; Chemistry and Materials Science ; Cholesterol, HDL - blood ; Cholesterol, LDL - blood ; Cricetinae ; Diacylglycerol O-Acyltransferase - genetics ; Diacylglycerol O-Acyltransferase - metabolism ; diacylglycerols ; Diet, High-Fat ; Dietary Supplements ; Fatty Acid Synthases - genetics ; Fatty Acid Synthases - metabolism ; Fatty Acids, Omega-3 - pharmacology ; fatty-acid synthase ; Female ; Food engineering ; gene expression ; glucose ; glucose tolerance ; hamsters ; high fat diet ; Hypertriglyceridemia - diet therapy ; Lactosylceramides - blood ; Life Sciences ; lipogenesis ; Lipogenesis - drug effects ; liver ; Liver - drug effects ; Liver - metabolism ; long chain polyunsaturated fatty acids ; Lysophospholipids - metabolism ; Male ; males ; Maternal Nutritional Physiological Phenomena ; mothers ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - metabolism ; muscles ; Nutrition ; Original Contribution ; progeny ; pups ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; secretion ; Sphingolipids - blood ; sphingosine ; Sphingosine - analogs & derivatives ; Sphingosine - metabolism ; stearoyl-CoA desaturase ; Stearoyl-CoA Desaturase - genetics ; Stearoyl-CoA Desaturase - metabolism ; Sterol Regulatory Element Binding Protein 1 - genetics ; Sterol Regulatory Element Binding Protein 1 - metabolism ; triacylglycerols ; Triglycerides - blood ; tumor necrosis factor-alpha ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - metabolism ; weaning</subject><ispartof>European journal of nutrition, 2016-03, Vol.55 (2), p.589-599</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>Springer-Verlag Berlin Heidelberg 2016</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c500t-82033efca7092283baefbd22285826ee2bb02b36f6c5880a2de4e21a8a443db03</citedby><cites>FETCH-LOGICAL-c500t-82033efca7092283baefbd22285826ee2bb02b36f6c5880a2de4e21a8a443db03</cites><orcidid>0000-0003-0058-6462 ; 0000-0001-6809-1488</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00394-015-0879-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00394-015-0879-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,777,781,882,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25787885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02631523$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Kasbi-Chadli, Fatima</creatorcontrib><creatorcontrib>Ferchaud-Roucher, Véronique</creatorcontrib><creatorcontrib>Krempf, Michel</creatorcontrib><creatorcontrib>Ouguerram, Khadija</creatorcontrib><title>Direct and maternal n-3 long-chain polyunsaturated fatty acid supplementation improved triglyceridemia and glycemia through the regulation of hepatic and muscle sphingolipid synthesis in offspring hamsters fed a high-fat diet</title><title>European journal of nutrition</title><addtitle>Eur J Nutr</addtitle><addtitle>Eur J Nutr</addtitle><description>PURPOSE: We recently reported that direct and maternal supplementation with n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) alleviates the metabolic disturbances in adult hamster pups fed with a high-fat diet (HFD). In this study, we hypothesized that these results involved a perinatal modulating effect of sphingolipids by n-3 LC-PUFA. METHODS: We studied the effect of direct and maternal n-3 LC-PUFA supplementation on sphingolipid contents in liver and muscle, hepatic triglycerides (TG) secretion and glucose tolerance. Offspring male hamsters born from supplemented (Cω) or unsupplemented (C) mothers were subjected after weaning to a HFD during 16 weeks, without (Cω-HF or C-HF) or with direct supplementation with n-3 LC-PUFA (C-HFω). RESULTS: Direct supplementation decreased sphingosine, sphinganine and ceramides in liver and decreased sphingosine, sphinganine, sphingosine-1-phosphate (S1P) and ceramides in muscle in C-HFω compared to C-HF (p < 0.05). Maternal supplementation decreased C20 ceramide and lactosylceramide in liver and sphinganine, S1P and lactosylceramide in muscle (p < 0.05). This supplementation tended to decrease glucosylceramide in liver (p < 0.06) and muscle (p < 0.07) in Cω-HF compared to C-HF. Direct supplementation increased glucose tolerance and decreased hepatic TG secretion and hepatic gene expression levels of diacylglycerol O-acyltransferase 2 (DGAT2), sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase, stearoyl-CoA desaturase-1 (SCD1) and tumor necrosis factor α (TNFα). Maternal supplementation decreased basal glycemia and hepatic TG secretion. We observed a positive correlation between hepatic TG secretion and hepatic ceramide (p = 0.0059), and between basal glycemia and hepatic ceramide (p = 0.04) or muscle lactosylceramide contents (p = 0.001). CONCLUSION: We observed an improvement of lipids and glucose metabolism in hamster with n-3 LC-PUFA direct supplementation and a decrease in glycemia and hepatic TG secretion with maternal supplementation. These results are probably related to a decrease in both lipogenesis and sphingolipid contents in liver and muscle.</description><subject>Adipose Tissue - drug effects</subject><subject>Adipose Tissue - metabolism</subject><subject>adults</subject><subject>Animals</subject><subject>Antigens, CD - blood</subject><subject>blood glucose</subject><subject>Blood Glucose - metabolism</subject><subject>ceramides</subject><subject>Ceramides - metabolism</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cholesterol, HDL - blood</subject><subject>Cholesterol, LDL - blood</subject><subject>Cricetinae</subject><subject>Diacylglycerol O-Acyltransferase - genetics</subject><subject>Diacylglycerol O-Acyltransferase - metabolism</subject><subject>diacylglycerols</subject><subject>Diet, High-Fat</subject><subject>Dietary Supplements</subject><subject>Fatty Acid Synthases - genetics</subject><subject>Fatty Acid Synthases - metabolism</subject><subject>Fatty Acids, Omega-3 - pharmacology</subject><subject>fatty-acid synthase</subject><subject>Female</subject><subject>Food engineering</subject><subject>gene expression</subject><subject>glucose</subject><subject>glucose tolerance</subject><subject>hamsters</subject><subject>high fat diet</subject><subject>Hypertriglyceridemia - diet therapy</subject><subject>Lactosylceramides - blood</subject><subject>Life Sciences</subject><subject>lipogenesis</subject><subject>Lipogenesis - drug effects</subject><subject>liver</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>long chain polyunsaturated fatty acids</subject><subject>Lysophospholipids - metabolism</subject><subject>Male</subject><subject>males</subject><subject>Maternal Nutritional Physiological Phenomena</subject><subject>mothers</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Muscle, Skeletal - metabolism</subject><subject>muscles</subject><subject>Nutrition</subject><subject>Original Contribution</subject><subject>progeny</subject><subject>pups</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>secretion</subject><subject>Sphingolipids - blood</subject><subject>sphingosine</subject><subject>Sphingosine - analogs & derivatives</subject><subject>Sphingosine - metabolism</subject><subject>stearoyl-CoA desaturase</subject><subject>Stearoyl-CoA Desaturase - genetics</subject><subject>Stearoyl-CoA Desaturase - metabolism</subject><subject>Sterol Regulatory Element Binding Protein 1 - genetics</subject><subject>Sterol Regulatory Element Binding Protein 1 - metabolism</subject><subject>triacylglycerols</subject><subject>Triglycerides - blood</subject><subject>tumor necrosis factor-alpha</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>weaning</subject><issn>1436-6207</issn><issn>1436-6215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9ks1u1TAQhSMEoqXwAGzAEhtYBMb2dZK7rMpPka7EArq2JskkceXEwXYq3cflTXCacoVYsPLY_s7MkX2y7CWH9xyg_BAA5H6XA1c5VOU-h0fZOd_JIi8EV49PNZRn2bMQbgFAyII_zc6EKquyqtR59uuj8dREhlPLRozkJ7RsyiWzburzZkAzsdnZ4zIFjItPRMs6jPHIsDEtC8s8WxppihiNm5gZZ-_uEhO96e2xIW9aGg3e978_WDdx8G7ph7QS89QvdhO7jg00p7rZ7CyhscTCPJipd9bM67zjlETBBGZWvguzT5dswDEk74F1aTSywfRDnlyy1lB8nj3p0AZ68bBeZDefP_24us4P3758vbo85I0CiHklQErqGixhL0Qla6SubkUqVSUKIlHXIGpZdEWjqgpQtLQjwbHC3U62NciL7N3Wd0Crk60R_VE7NPr68qDXMxCF5ErIO57YtxubXuvnQiHq0YSGrMWJ3BI0L4tKqRJ2K_rmH_TWLesvrVTJQSlersP5RjXeheCpOzngoNes6C0rOmVFr1nRq-bVQ-elHqk9Kf6EIwFiA7ZXJv_X6P90fb2JOnQae2-CvvkugBcpfYkolPwNi5LX3g</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Kasbi-Chadli, Fatima</creator><creator>Ferchaud-Roucher, Véronique</creator><creator>Krempf, Michel</creator><creator>Ouguerram, Khadija</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RQ</scope><scope>7RV</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-0058-6462</orcidid><orcidid>https://orcid.org/0000-0001-6809-1488</orcidid></search><sort><creationdate>20160301</creationdate><title>Direct and maternal n-3 long-chain polyunsaturated fatty acid supplementation improved triglyceridemia and glycemia through the regulation of hepatic and muscle sphingolipid synthesis in offspring hamsters fed a high-fat diet</title><author>Kasbi-Chadli, Fatima ; Ferchaud-Roucher, Véronique ; Krempf, Michel ; Ouguerram, Khadija</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-82033efca7092283baefbd22285826ee2bb02b36f6c5880a2de4e21a8a443db03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adipose Tissue - drug effects</topic><topic>Adipose Tissue - metabolism</topic><topic>adults</topic><topic>Animals</topic><topic>Antigens, CD - blood</topic><topic>blood glucose</topic><topic>Blood Glucose - metabolism</topic><topic>ceramides</topic><topic>Ceramides - metabolism</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cholesterol, HDL - blood</topic><topic>Cholesterol, LDL - blood</topic><topic>Cricetinae</topic><topic>Diacylglycerol O-Acyltransferase - genetics</topic><topic>Diacylglycerol O-Acyltransferase - metabolism</topic><topic>diacylglycerols</topic><topic>Diet, High-Fat</topic><topic>Dietary Supplements</topic><topic>Fatty Acid Synthases - genetics</topic><topic>Fatty Acid Synthases - metabolism</topic><topic>Fatty Acids, Omega-3 - pharmacology</topic><topic>fatty-acid synthase</topic><topic>Female</topic><topic>Food engineering</topic><topic>gene expression</topic><topic>glucose</topic><topic>glucose tolerance</topic><topic>hamsters</topic><topic>high fat diet</topic><topic>Hypertriglyceridemia - diet therapy</topic><topic>Lactosylceramides - blood</topic><topic>Life Sciences</topic><topic>lipogenesis</topic><topic>Lipogenesis - drug effects</topic><topic>liver</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>long chain polyunsaturated fatty acids</topic><topic>Lysophospholipids - metabolism</topic><topic>Male</topic><topic>males</topic><topic>Maternal Nutritional Physiological Phenomena</topic><topic>mothers</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Muscle, Skeletal - metabolism</topic><topic>muscles</topic><topic>Nutrition</topic><topic>Original Contribution</topic><topic>progeny</topic><topic>pups</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>secretion</topic><topic>Sphingolipids - blood</topic><topic>sphingosine</topic><topic>Sphingosine - analogs & derivatives</topic><topic>Sphingosine - metabolism</topic><topic>stearoyl-CoA desaturase</topic><topic>Stearoyl-CoA Desaturase - genetics</topic><topic>Stearoyl-CoA Desaturase - metabolism</topic><topic>Sterol Regulatory Element Binding Protein 1 - genetics</topic><topic>Sterol Regulatory Element Binding Protein 1 - metabolism</topic><topic>triacylglycerols</topic><topic>Triglycerides - blood</topic><topic>tumor necrosis factor-alpha</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>weaning</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kasbi-Chadli, Fatima</creatorcontrib><creatorcontrib>Ferchaud-Roucher, Véronique</creatorcontrib><creatorcontrib>Krempf, Michel</creatorcontrib><creatorcontrib>Ouguerram, Khadija</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Career & Technical Education Database</collection><collection>Nursing & Allied Health Database</collection><collection>Physical Education Index</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>European journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kasbi-Chadli, Fatima</au><au>Ferchaud-Roucher, Véronique</au><au>Krempf, Michel</au><au>Ouguerram, Khadija</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct and maternal n-3 long-chain polyunsaturated fatty acid supplementation improved triglyceridemia and glycemia through the regulation of hepatic and muscle sphingolipid synthesis in offspring hamsters fed a high-fat diet</atitle><jtitle>European journal of nutrition</jtitle><stitle>Eur J Nutr</stitle><addtitle>Eur J Nutr</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>55</volume><issue>2</issue><spage>589</spage><epage>599</epage><pages>589-599</pages><issn>1436-6207</issn><eissn>1436-6215</eissn><abstract>PURPOSE: We recently reported that direct and maternal supplementation with n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) alleviates the metabolic disturbances in adult hamster pups fed with a high-fat diet (HFD). In this study, we hypothesized that these results involved a perinatal modulating effect of sphingolipids by n-3 LC-PUFA. METHODS: We studied the effect of direct and maternal n-3 LC-PUFA supplementation on sphingolipid contents in liver and muscle, hepatic triglycerides (TG) secretion and glucose tolerance. Offspring male hamsters born from supplemented (Cω) or unsupplemented (C) mothers were subjected after weaning to a HFD during 16 weeks, without (Cω-HF or C-HF) or with direct supplementation with n-3 LC-PUFA (C-HFω). RESULTS: Direct supplementation decreased sphingosine, sphinganine and ceramides in liver and decreased sphingosine, sphinganine, sphingosine-1-phosphate (S1P) and ceramides in muscle in C-HFω compared to C-HF (p < 0.05). Maternal supplementation decreased C20 ceramide and lactosylceramide in liver and sphinganine, S1P and lactosylceramide in muscle (p < 0.05). This supplementation tended to decrease glucosylceramide in liver (p < 0.06) and muscle (p < 0.07) in Cω-HF compared to C-HF. Direct supplementation increased glucose tolerance and decreased hepatic TG secretion and hepatic gene expression levels of diacylglycerol O-acyltransferase 2 (DGAT2), sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase, stearoyl-CoA desaturase-1 (SCD1) and tumor necrosis factor α (TNFα). Maternal supplementation decreased basal glycemia and hepatic TG secretion. We observed a positive correlation between hepatic TG secretion and hepatic ceramide (p = 0.0059), and between basal glycemia and hepatic ceramide (p = 0.04) or muscle lactosylceramide contents (p = 0.001). CONCLUSION: We observed an improvement of lipids and glucose metabolism in hamster with n-3 LC-PUFA direct supplementation and a decrease in glycemia and hepatic TG secretion with maternal supplementation. These results are probably related to a decrease in both lipogenesis and sphingolipid contents in liver and muscle.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25787885</pmid><doi>10.1007/s00394-015-0879-0</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-0058-6462</orcidid><orcidid>https://orcid.org/0000-0001-6809-1488</orcidid></addata></record> |
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| identifier | ISSN: 1436-6207 |
| ispartof | European journal of nutrition, 2016-03, Vol.55 (2), p.589-599 |
| issn | 1436-6207 1436-6215 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02631523v1 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adipose Tissue - drug effects Adipose Tissue - metabolism adults Animals Antigens, CD - blood blood glucose Blood Glucose - metabolism ceramides Ceramides - metabolism Chemistry Chemistry and Materials Science Cholesterol, HDL - blood Cholesterol, LDL - blood Cricetinae Diacylglycerol O-Acyltransferase - genetics Diacylglycerol O-Acyltransferase - metabolism diacylglycerols Diet, High-Fat Dietary Supplements Fatty Acid Synthases - genetics Fatty Acid Synthases - metabolism Fatty Acids, Omega-3 - pharmacology fatty-acid synthase Female Food engineering gene expression glucose glucose tolerance hamsters high fat diet Hypertriglyceridemia - diet therapy Lactosylceramides - blood Life Sciences lipogenesis Lipogenesis - drug effects liver Liver - drug effects Liver - metabolism long chain polyunsaturated fatty acids Lysophospholipids - metabolism Male males Maternal Nutritional Physiological Phenomena mothers Muscle, Skeletal - drug effects Muscle, Skeletal - metabolism muscles Nutrition Original Contribution progeny pups RNA, Messenger - genetics RNA, Messenger - metabolism secretion Sphingolipids - blood sphingosine Sphingosine - analogs & derivatives Sphingosine - metabolism stearoyl-CoA desaturase Stearoyl-CoA Desaturase - genetics Stearoyl-CoA Desaturase - metabolism Sterol Regulatory Element Binding Protein 1 - genetics Sterol Regulatory Element Binding Protein 1 - metabolism triacylglycerols Triglycerides - blood tumor necrosis factor-alpha Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism weaning |
| title | Direct and maternal n-3 long-chain polyunsaturated fatty acid supplementation improved triglyceridemia and glycemia through the regulation of hepatic and muscle sphingolipid synthesis in offspring hamsters fed a high-fat diet |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T08%3A09%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Direct%20and%20maternal%20n-3%20long-chain%20polyunsaturated%20fatty%20acid%20supplementation%20improved%20triglyceridemia%20and%20glycemia%20through%20the%20regulation%20of%20hepatic%20and%20muscle%20sphingolipid%20synthesis%20in%20offspring%20hamsters%20fed%20a%20high-fat%20diet&rft.jtitle=European%20journal%20of%20nutrition&rft.au=Kasbi-Chadli,%20Fatima&rft.date=2016-03-01&rft.volume=55&rft.issue=2&rft.spage=589&rft.epage=599&rft.pages=589-599&rft.issn=1436-6207&rft.eissn=1436-6215&rft_id=info:doi/10.1007/s00394-015-0879-0&rft_dat=%3Cproquest_hal_p%3E1768557041%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1771055170&rft_id=info:pmid/25787885&rfr_iscdi=true |