The tomato Ethylene Response Factor Sl-ERF.B3 integrates ethylene and auxin signaling via direct regulation of Sl-Aux/IAA27
Plant growth and development is coordinated by complex networks of interacting hormones, and cross-talk between ethylene and auxin signaling is essential for a wide range of plant developmental processes. Nevertheless, the molecular links underlying the interaction between the two hormones remain po...
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| Veröffentlicht in: | The New phytologist 2018-07, Vol.219 (2), p.631-640 |
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| creator | Liu, Mingchun Chen, Yao Chen, Ya Shin, Jun‐Hye Mila, Isabelle Audran, Corinne Zouine, Mohamed Pirrello, Julien Bouzayen, Mondher |
| description | Plant growth and development is coordinated by complex networks of interacting hormones, and cross-talk between ethylene and auxin signaling is essential for a wide range of plant developmental processes. Nevertheless, the molecular links underlying the interaction between the two hormones remain poorly understood.
In order to decipher the cross-talk between the Ethylene Response Factor Sl-ERF.B3 and Sl-IAA27, mediating ethylene and auxin signaling, respectively, we combined reverse genetic approaches, physiological methods, transactivation experiments and electrophoretic mobility shift assays.
Sl-ERF.B3 is responsive to both ethylene and auxin and ectopic expression of its dominant repressor version (ERF.B3-SRDX) results in impaired sensitivity to auxin with phenotypes recalling those previously reported for Sl-IAA27 downregulated tomato lines. The expression of Sl-IAA27 is dramatically reduced in the ERF.B3-SRDX lines and Sl-ERF.B3 is shown to regulate the expression of Sl-IAA27 via direct binding to its promoter.
The data support a model in which the ethylene-responsive Sl-ERF.B3 integrates ethylene and auxin signaling via regulation of the expression of the auxin signaling component Sl-IAA27. The study uncovers a molecular mechanism that links ethylene and auxin signaling in tomato. |
| doi_str_mv | 10.1111/nph.15165 |
| format | Article |
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In order to decipher the cross-talk between the Ethylene Response Factor Sl-ERF.B3 and Sl-IAA27, mediating ethylene and auxin signaling, respectively, we combined reverse genetic approaches, physiological methods, transactivation experiments and electrophoretic mobility shift assays.
Sl-ERF.B3 is responsive to both ethylene and auxin and ectopic expression of its dominant repressor version (ERF.B3-SRDX) results in impaired sensitivity to auxin with phenotypes recalling those previously reported for Sl-IAA27 downregulated tomato lines. The expression of Sl-IAA27 is dramatically reduced in the ERF.B3-SRDX lines and Sl-ERF.B3 is shown to regulate the expression of Sl-IAA27 via direct binding to its promoter.
The data support a model in which the ethylene-responsive Sl-ERF.B3 integrates ethylene and auxin signaling via regulation of the expression of the auxin signaling component Sl-IAA27. The study uncovers a molecular mechanism that links ethylene and auxin signaling in tomato.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.15165</identifier><identifier>PMID: 29701899</identifier><language>eng</language><publisher>England: New Phytologist Trust</publisher><subject>Aux/IAA ; auxin signaling ; ethylene ; Ethylene Response Factor (ERF) ; hormonal cross‐talk ; Life Sciences ; Solanum lycopersicum (tomato) ; Vegetal Biology</subject><ispartof>The New phytologist, 2018-07, Vol.219 (2), p.631-640</ispartof><rights>2018 New Phytologist Trust</rights><rights>2018 The Authors. New Phytologist © 2018 New Phytologist Trust</rights><rights>2018 The Authors. New Phytologist © 2018 New Phytologist Trust.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-4981-9389 ; 0000-0002-3083-8088 ; 0000-0001-7630-1449</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/90022596$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/90022596$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,1411,1427,27901,27902,45550,45551,46384,46808,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29701899$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02626490$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Mingchun</creatorcontrib><creatorcontrib>Chen, Yao</creatorcontrib><creatorcontrib>Chen, Ya</creatorcontrib><creatorcontrib>Shin, Jun‐Hye</creatorcontrib><creatorcontrib>Mila, Isabelle</creatorcontrib><creatorcontrib>Audran, Corinne</creatorcontrib><creatorcontrib>Zouine, Mohamed</creatorcontrib><creatorcontrib>Pirrello, Julien</creatorcontrib><creatorcontrib>Bouzayen, Mondher</creatorcontrib><title>The tomato Ethylene Response Factor Sl-ERF.B3 integrates ethylene and auxin signaling via direct regulation of Sl-Aux/IAA27</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>Plant growth and development is coordinated by complex networks of interacting hormones, and cross-talk between ethylene and auxin signaling is essential for a wide range of plant developmental processes. Nevertheless, the molecular links underlying the interaction between the two hormones remain poorly understood.
In order to decipher the cross-talk between the Ethylene Response Factor Sl-ERF.B3 and Sl-IAA27, mediating ethylene and auxin signaling, respectively, we combined reverse genetic approaches, physiological methods, transactivation experiments and electrophoretic mobility shift assays.
Sl-ERF.B3 is responsive to both ethylene and auxin and ectopic expression of its dominant repressor version (ERF.B3-SRDX) results in impaired sensitivity to auxin with phenotypes recalling those previously reported for Sl-IAA27 downregulated tomato lines. The expression of Sl-IAA27 is dramatically reduced in the ERF.B3-SRDX lines and Sl-ERF.B3 is shown to regulate the expression of Sl-IAA27 via direct binding to its promoter.
The data support a model in which the ethylene-responsive Sl-ERF.B3 integrates ethylene and auxin signaling via regulation of the expression of the auxin signaling component Sl-IAA27. The study uncovers a molecular mechanism that links ethylene and auxin signaling in tomato.</description><subject>Aux/IAA</subject><subject>auxin signaling</subject><subject>ethylene</subject><subject>Ethylene Response Factor (ERF)</subject><subject>hormonal cross‐talk</subject><subject>Life Sciences</subject><subject>Solanum lycopersicum (tomato)</subject><subject>Vegetal Biology</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNo9kctu2zAQRYmiReOmXfQDWnCZLmTzYVLiUg3sOoDRFnkA3RGUNLQYyKQrSkmM_nzpOPFsZjBz7l3MRegzJVOaauZ37ZQKKsUbNKFzqbKC8vwtmhDCikzO5Z8z9CHGe0KIEpK9R2dM5YQWSk3Qv9sW8BC2Zgh4MbT7Djzga4i74CPgpamH0OObLltcL6ffOXZ-gE1vBogYXmnjG2zGJ-dxdBtvOuc3-MEZ3Lge6gH3sBk7M7jgcbAHq3J8ml2VJcs_onfWdBE-vfRzdLdc3F6usvWvH1eX5TprOclFVnEjqlqJmtlC1TIXglorQFWqKZhVlRRybmubNwVVyppGcEg0cKGosYoLfo6-HX1b0-ld77am3-tgnF6Va33YESaZnCvyQBN7cWR3ffg7Qhz01sUaus54CGPUjHDGVU45SejXF3SsttCcnF-fm4DZEXh0HexPd0r0ITWdUtPPqemfv1fPQ1J8OSruY_r7SaFSkEwoyf8DJAqSSA</recordid><startdate>201807</startdate><enddate>201807</enddate><creator>Liu, Mingchun</creator><creator>Chen, Yao</creator><creator>Chen, Ya</creator><creator>Shin, Jun‐Hye</creator><creator>Mila, Isabelle</creator><creator>Audran, Corinne</creator><creator>Zouine, Mohamed</creator><creator>Pirrello, Julien</creator><creator>Bouzayen, Mondher</creator><general>New Phytologist Trust</general><general>Wiley</general><scope>NPM</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-4981-9389</orcidid><orcidid>https://orcid.org/0000-0002-3083-8088</orcidid><orcidid>https://orcid.org/0000-0001-7630-1449</orcidid></search><sort><creationdate>201807</creationdate><title>The tomato Ethylene Response Factor Sl-ERF.B3 integrates ethylene and auxin signaling via direct regulation of Sl-Aux/IAA27</title><author>Liu, Mingchun ; Chen, Yao ; Chen, Ya ; Shin, Jun‐Hye ; Mila, Isabelle ; Audran, Corinne ; Zouine, Mohamed ; Pirrello, Julien ; Bouzayen, Mondher</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h3075-b3a5bc95c2f89c67551ff5e9b9d82f9b6564fcf7d8199fad53ec95e3591af9353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Aux/IAA</topic><topic>auxin signaling</topic><topic>ethylene</topic><topic>Ethylene Response Factor (ERF)</topic><topic>hormonal cross‐talk</topic><topic>Life Sciences</topic><topic>Solanum lycopersicum (tomato)</topic><topic>Vegetal Biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Mingchun</creatorcontrib><creatorcontrib>Chen, Yao</creatorcontrib><creatorcontrib>Chen, Ya</creatorcontrib><creatorcontrib>Shin, Jun‐Hye</creatorcontrib><creatorcontrib>Mila, Isabelle</creatorcontrib><creatorcontrib>Audran, Corinne</creatorcontrib><creatorcontrib>Zouine, Mohamed</creatorcontrib><creatorcontrib>Pirrello, Julien</creatorcontrib><creatorcontrib>Bouzayen, Mondher</creatorcontrib><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Mingchun</au><au>Chen, Yao</au><au>Chen, Ya</au><au>Shin, Jun‐Hye</au><au>Mila, Isabelle</au><au>Audran, Corinne</au><au>Zouine, Mohamed</au><au>Pirrello, Julien</au><au>Bouzayen, Mondher</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The tomato Ethylene Response Factor Sl-ERF.B3 integrates ethylene and auxin signaling via direct regulation of Sl-Aux/IAA27</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2018-07</date><risdate>2018</risdate><volume>219</volume><issue>2</issue><spage>631</spage><epage>640</epage><pages>631-640</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Plant growth and development is coordinated by complex networks of interacting hormones, and cross-talk between ethylene and auxin signaling is essential for a wide range of plant developmental processes. Nevertheless, the molecular links underlying the interaction between the two hormones remain poorly understood.
In order to decipher the cross-talk between the Ethylene Response Factor Sl-ERF.B3 and Sl-IAA27, mediating ethylene and auxin signaling, respectively, we combined reverse genetic approaches, physiological methods, transactivation experiments and electrophoretic mobility shift assays.
Sl-ERF.B3 is responsive to both ethylene and auxin and ectopic expression of its dominant repressor version (ERF.B3-SRDX) results in impaired sensitivity to auxin with phenotypes recalling those previously reported for Sl-IAA27 downregulated tomato lines. The expression of Sl-IAA27 is dramatically reduced in the ERF.B3-SRDX lines and Sl-ERF.B3 is shown to regulate the expression of Sl-IAA27 via direct binding to its promoter.
The data support a model in which the ethylene-responsive Sl-ERF.B3 integrates ethylene and auxin signaling via regulation of the expression of the auxin signaling component Sl-IAA27. The study uncovers a molecular mechanism that links ethylene and auxin signaling in tomato.</abstract><cop>England</cop><pub>New Phytologist Trust</pub><pmid>29701899</pmid><doi>10.1111/nph.15165</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-4981-9389</orcidid><orcidid>https://orcid.org/0000-0002-3083-8088</orcidid><orcidid>https://orcid.org/0000-0001-7630-1449</orcidid></addata></record> |
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| source | Jstor Complete Legacy; Wiley Free Content; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Aux/IAA auxin signaling ethylene Ethylene Response Factor (ERF) hormonal cross‐talk Life Sciences Solanum lycopersicum (tomato) Vegetal Biology |
| title | The tomato Ethylene Response Factor Sl-ERF.B3 integrates ethylene and auxin signaling via direct regulation of Sl-Aux/IAA27 |
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