Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm
Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm f...
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| creator | Rakha, B.A. Ansari, M.S. Akhter, S. Santiago-Moreno, J. Blesbois, E. |
| description | Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red jungle fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red jungle fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 ± 2.5%), plasma membrane integrity (66.3 ± 2.4%), viability (58.8 ± 1.3%) and acrosomal integrity (60.0.8 ± 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 ± 0.7, 21.6 ± 1.2), percent fertility (55.9 ± 4.4, 46.5 ± 2.2), no. of hatched chicks (23.6 ± 1.0, 17.2 ± 1.0), percent hatch (49.5 ± 3.2, 37.1 ± 2.5%) and hatchability of the fertile eggs (89.4 ± 2.2, 79.7 ± 3.7) were recorded higher (P |
| doi_str_mv | 10.1016/j.theriogenology.2018.06.015 |
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•Effect of egg yolk levels in extender were evaluated on the freezability of Indian red jungle fowl sperm.•Egg yolk at 15% in extender improved semen quality and fertility of Indian red jungle fowl.•Egg yolk can be used as an alternate to glycerol for cryopreservation of Indian red jungle fowl sperm.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2018.06.015</identifier><identifier>PMID: 30007173</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acrosome ; Animals ; Chickens ; Computer Science ; Cryo-damage ; Cryopreservation - methods ; Cryopreservation - veterinary ; Cryoprotective Agents - chemistry ; Cryoprotective Agents - pharmacology ; Egg Yolk - chemistry ; Fertility ; Life Sciences ; Male ; Motility ; Rooster ; Semen Analysis - veterinary ; Semen Preservation - methods ; Semen Preservation - veterinary ; Sperm Motility - drug effects ; Sperm viability</subject><ispartof>Theriogenology, 2018-10, Vol.119, p.150-155</ispartof><rights>2018 Elsevier Inc.</rights><rights>Copyright © 2018 Elsevier Inc. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-57147a97b2559b936471cd47d2f9e297bcbe4678d4ba1f97b9dfcd581fa8ee6a3</citedby><cites>FETCH-LOGICAL-c420t-57147a97b2559b936471cd47d2f9e297bcbe4678d4ba1f97b9dfcd581fa8ee6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0093691X18303789$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30007173$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02622698$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Rakha, B.A.</creatorcontrib><creatorcontrib>Ansari, M.S.</creatorcontrib><creatorcontrib>Akhter, S.</creatorcontrib><creatorcontrib>Santiago-Moreno, J.</creatorcontrib><creatorcontrib>Blesbois, E.</creatorcontrib><title>Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red jungle fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red jungle fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 ± 2.5%), plasma membrane integrity (66.3 ± 2.4%), viability (58.8 ± 1.3%) and acrosomal integrity (60.0.8 ± 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 ± 0.7, 21.6 ± 1.2), percent fertility (55.9 ± 4.4, 46.5 ± 2.2), no. of hatched chicks (23.6 ± 1.0, 17.2 ± 1.0), percent hatch (49.5 ± 3.2, 37.1 ± 2.5%) and hatchability of the fertile eggs (89.4 ± 2.2, 79.7 ± 3.7) were recorded higher (P < 0.05) with semen cryopreserved with 15% egg yolk compared to control (20% glycerol). It is concluded that 15% egg yolk can be used in cryopreservation protocol of Indian red jungle fowl sperm.
•Effect of egg yolk levels in extender were evaluated on the freezability of Indian red jungle fowl sperm.•Egg yolk at 15% in extender improved semen quality and fertility of Indian red jungle fowl.•Egg yolk can be used as an alternate to glycerol for cryopreservation of Indian red jungle fowl sperm.</description><subject>Acrosome</subject><subject>Animals</subject><subject>Chickens</subject><subject>Computer Science</subject><subject>Cryo-damage</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotective Agents - chemistry</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Egg Yolk - chemistry</subject><subject>Fertility</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Motility</subject><subject>Rooster</subject><subject>Semen Analysis - veterinary</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Sperm Motility - drug effects</subject><subject>Sperm viability</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAQgC0EokvhFZAPHNpDgu0kdixxqVb0R1qJC0iIi-XE46wXJ17spNW-Dc_Ck-FlSyVunGY8-mZGng-hd5SUlFD-flfOW4guDDAFH4ZDyQhtS8JLQptnaEVbIYuKVfQ5WhEiq4JL-vUMvUppRwipOKcv0VmVU0FFtULf1vEQ9jHM0M96mjFYm7OEg8UwDPgQ_HccJnw3GacnHMHg3TINHrANDx5f3Gjvl_Tr5_An4nGJw9Zd4rSHOL5GL6z2Cd48xnP05frj5_Vtsfl0c7e-2hR9zchcNILWQkvRsaaRnax4LWhvamGYlcByve-g5qI1daepzW9pbG-allrdAnBdnaPL09yt9mof3ajjQQXt1O3VRh1rhHHGuGzvaWYvTmz-8o8F0qxGl3rwXk8QlqQYEYTVohFVRj-c0D6GlCLYp9mUqKMJtVP_mlBHE4pwlU3k9rePm5ZuBPPU_Pf0Gbg-AZBvc-8gqtQ7mHowLmYFygT3f5t-A_Igo-M</recordid><startdate>20181001</startdate><enddate>20181001</enddate><creator>Rakha, B.A.</creator><creator>Ansari, M.S.</creator><creator>Akhter, S.</creator><creator>Santiago-Moreno, J.</creator><creator>Blesbois, E.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20181001</creationdate><title>Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm</title><author>Rakha, B.A. ; Ansari, M.S. ; Akhter, S. ; Santiago-Moreno, J. ; Blesbois, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-57147a97b2559b936471cd47d2f9e297bcbe4678d4ba1f97b9dfcd581fa8ee6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acrosome</topic><topic>Animals</topic><topic>Chickens</topic><topic>Computer Science</topic><topic>Cryo-damage</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - veterinary</topic><topic>Cryoprotective Agents - chemistry</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Egg Yolk - chemistry</topic><topic>Fertility</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Motility</topic><topic>Rooster</topic><topic>Semen Analysis - veterinary</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Sperm Motility - drug effects</topic><topic>Sperm viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rakha, B.A.</creatorcontrib><creatorcontrib>Ansari, M.S.</creatorcontrib><creatorcontrib>Akhter, S.</creatorcontrib><creatorcontrib>Santiago-Moreno, J.</creatorcontrib><creatorcontrib>Blesbois, E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rakha, B.A.</au><au>Ansari, M.S.</au><au>Akhter, S.</au><au>Santiago-Moreno, J.</au><au>Blesbois, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2018-10-01</date><risdate>2018</risdate><volume>119</volume><spage>150</spage><epage>155</epage><pages>150-155</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red jungle fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red jungle fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 ± 2.5%), plasma membrane integrity (66.3 ± 2.4%), viability (58.8 ± 1.3%) and acrosomal integrity (60.0.8 ± 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 ± 0.7, 21.6 ± 1.2), percent fertility (55.9 ± 4.4, 46.5 ± 2.2), no. of hatched chicks (23.6 ± 1.0, 17.2 ± 1.0), percent hatch (49.5 ± 3.2, 37.1 ± 2.5%) and hatchability of the fertile eggs (89.4 ± 2.2, 79.7 ± 3.7) were recorded higher (P < 0.05) with semen cryopreserved with 15% egg yolk compared to control (20% glycerol). It is concluded that 15% egg yolk can be used in cryopreservation protocol of Indian red jungle fowl sperm.
•Effect of egg yolk levels in extender were evaluated on the freezability of Indian red jungle fowl sperm.•Egg yolk at 15% in extender improved semen quality and fertility of Indian red jungle fowl.•Egg yolk can be used as an alternate to glycerol for cryopreservation of Indian red jungle fowl sperm.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>30007173</pmid><doi>10.1016/j.theriogenology.2018.06.015</doi><tpages>6</tpages></addata></record> |
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| subjects | Acrosome Animals Chickens Computer Science Cryo-damage Cryopreservation - methods Cryopreservation - veterinary Cryoprotective Agents - chemistry Cryoprotective Agents - pharmacology Egg Yolk - chemistry Fertility Life Sciences Male Motility Rooster Semen Analysis - veterinary Semen Preservation - methods Semen Preservation - veterinary Sperm Motility - drug effects Sperm viability |
| title | Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm |
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