Characterization of isolates of Listeria monocytogenes from sludge using pulsed-field gel electrophoresis and virulence assays
To study the diversity and virulence of Listeria monocytogenes isolated from sludge. A total of 60 isolates of L. monocytogenes from sludge were characterized by serotyping, PFGE typing and using in vitro and in vivo virulence assays. The PFGE patterns were compared with those of food and human isol...
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| Veröffentlicht in: | Journal of applied microbiology 2010-04, Vol.108 (4), p.1380-1388 |
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| container_title | Journal of applied microbiology |
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| creator | Kerouanton, A Roche, S.M Marault, M Velge, P Pourcher, A.-M Brisabois, A Federighi, M Garrec, N |
| description | To study the diversity and virulence of Listeria monocytogenes isolated from sludge. A total of 60 isolates of L. monocytogenes from sludge were characterized by serotyping, PFGE typing and using in vitro and in vivo virulence assays. The PFGE patterns were compared with those of food and human isolates to determine whether specific group clones are associated with environmental samples. The 60 isolates gave 44 different combined ApaI/AscI PFGE patterns. The PFGE patterns of most isolates were similar or very similar to those of epidemic isolates. The majority (93%) of isolates were found to be virulent by plaque-forming assay and by mouse virulence assay. Our findings suggest that L. monocytogenes strains found in non-sanitized sludge are virulent and represent a potential health hazard. Although no case of listeriosis related to sludge spread onto agricultural land has been reported, particular attention to this pathogen is needed. This is the first study dealing with the characterization of L. monocytogenes isolates from non-sanitized sludge samples by molecular typing methods and in vitro and in vivo virulence assays. Our findings provide relevant information for evaluating the health risks associated with spreading sludge onto agricultural land. |
| doi_str_mv | 10.1111/j.1365-2672.2009.04531.x |
| format | Article |
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A total of 60 isolates of L. monocytogenes from sludge were characterized by serotyping, PFGE typing and using in vitro and in vivo virulence assays. The PFGE patterns were compared with those of food and human isolates to determine whether specific group clones are associated with environmental samples. The 60 isolates gave 44 different combined ApaI/AscI PFGE patterns. The PFGE patterns of most isolates were similar or very similar to those of epidemic isolates. The majority (93%) of isolates were found to be virulent by plaque-forming assay and by mouse virulence assay. Our findings suggest that L. monocytogenes strains found in non-sanitized sludge are virulent and represent a potential health hazard. Although no case of listeriosis related to sludge spread onto agricultural land has been reported, particular attention to this pathogen is needed. This is the first study dealing with the characterization of L. monocytogenes isolates from non-sanitized sludge samples by molecular typing methods and in vitro and in vivo virulence assays. Our findings provide relevant information for evaluating the health risks associated with spreading sludge onto agricultural land.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/j.1365-2672.2009.04531.x</identifier><identifier>PMID: 19796096</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Animals ; Biological and medical sciences ; Electrophoresis, Gel, Pulsed-Field ; Environmental Sciences ; Fundamental and applied biological sciences. Psychology ; Humans ; Listeria monocytogenes ; Listeria monocytogenes - classification ; Listeria monocytogenes - genetics ; Listeria monocytogenes - isolation & purification ; Listeria monocytogenes - pathogenicity ; Mice ; Microbiology ; Molecular Typing ; PFGE subtyping ; Serotyping ; Sewage - microbiology ; sludge ; Virulence ; virulence assay</subject><ispartof>Journal of applied microbiology, 2010-04, Vol.108 (4), p.1380-1388</ispartof><rights>Journal compilation © 2009 The Society for Applied Microbiology. No claim to original French government works</rights><rights>2015 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4881-b61e462ef6ac6434464b10b765434358ace9bf352e53425ffcbc04cced45c0183</citedby><cites>FETCH-LOGICAL-c4881-b61e462ef6ac6434464b10b765434358ace9bf352e53425ffcbc04cced45c0183</cites><orcidid>0000-0002-1108-6490 ; 0000-0001-5770-7443 ; 0000-0003-0452-2060 ; 0000-0002-8201-0194</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2672.2009.04531.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2672.2009.04531.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,781,785,886,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22537217$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19796096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02594050$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Kerouanton, A</creatorcontrib><creatorcontrib>Roche, S.M</creatorcontrib><creatorcontrib>Marault, M</creatorcontrib><creatorcontrib>Velge, P</creatorcontrib><creatorcontrib>Pourcher, A.-M</creatorcontrib><creatorcontrib>Brisabois, A</creatorcontrib><creatorcontrib>Federighi, M</creatorcontrib><creatorcontrib>Garrec, N</creatorcontrib><title>Characterization of isolates of Listeria monocytogenes from sludge using pulsed-field gel electrophoresis and virulence assays</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>To study the diversity and virulence of Listeria monocytogenes isolated from sludge. A total of 60 isolates of L. monocytogenes from sludge were characterized by serotyping, PFGE typing and using in vitro and in vivo virulence assays. The PFGE patterns were compared with those of food and human isolates to determine whether specific group clones are associated with environmental samples. The 60 isolates gave 44 different combined ApaI/AscI PFGE patterns. The PFGE patterns of most isolates were similar or very similar to those of epidemic isolates. The majority (93%) of isolates were found to be virulent by plaque-forming assay and by mouse virulence assay. Our findings suggest that L. monocytogenes strains found in non-sanitized sludge are virulent and represent a potential health hazard. Although no case of listeriosis related to sludge spread onto agricultural land has been reported, particular attention to this pathogen is needed. This is the first study dealing with the characterization of L. monocytogenes isolates from non-sanitized sludge samples by molecular typing methods and in vitro and in vivo virulence assays. Our findings provide relevant information for evaluating the health risks associated with spreading sludge onto agricultural land.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Environmental Sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - classification</subject><subject>Listeria monocytogenes - genetics</subject><subject>Listeria monocytogenes - isolation & purification</subject><subject>Listeria monocytogenes - pathogenicity</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Molecular Typing</subject><subject>PFGE subtyping</subject><subject>Serotyping</subject><subject>Sewage - microbiology</subject><subject>sludge</subject><subject>Virulence</subject><subject>virulence assay</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2P0zAQhiMEYpeFvwC-oBWHFH8nOXCoKmBBRRxgz5bjjFtXblzsZNly4LfjbKpyBF889jzvzFiviwIRvCB5vd0tCJOipLKiC4pxs8BcMLK4f1RcnhOPH2JeClzRi-JZSjuMCcNCPi0uSFM1Ejfysvi92uqozQDR_dKDCz0KFrkUvB4gTfHapSmp0T70wRyHsIE-Z2wMe5T82G0Ajcn1G3QYfYKutA58hzbgEXgwQwyHbYiQXEK679Cdi6OH3gDSKeljel48sTrrXpz2q-L2w_vvq5ty_fXjp9VyXRpe16RsJQEuKVipjeSMc8lbgttKinxgotYGmtYyQUEwToW1pjWYGwMdFwaTml0Vb-a6W-3VIbq9jkcVtFM3y7Wa7jAVDccC35HMXs_sIYYfI6RB7V0y4L3uIYxJVVwyLKnA_yYZazhv6qlmPZMmhpQi2PMQBKvJUrVTk3Nqck5NlqoHS9V9lr48NRnbPXR_hScPM_D6BOhktLdR98alM0epYBUlVebezdxP5-H43wOoz8svU5T1r2a91UHpTcw9br_R6U-R_ELcUPYH_FHG2Q</recordid><startdate>201004</startdate><enddate>201004</enddate><creator>Kerouanton, A</creator><creator>Roche, S.M</creator><creator>Marault, M</creator><creator>Velge, P</creator><creator>Pourcher, A.-M</creator><creator>Brisabois, A</creator><creator>Federighi, M</creator><creator>Garrec, N</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Wiley</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>7U1</scope><scope>7U2</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-1108-6490</orcidid><orcidid>https://orcid.org/0000-0001-5770-7443</orcidid><orcidid>https://orcid.org/0000-0003-0452-2060</orcidid><orcidid>https://orcid.org/0000-0002-8201-0194</orcidid></search><sort><creationdate>201004</creationdate><title>Characterization of isolates of Listeria monocytogenes from sludge using pulsed-field gel electrophoresis and virulence assays</title><author>Kerouanton, A ; Roche, S.M ; Marault, M ; Velge, P ; Pourcher, A.-M ; Brisabois, A ; Federighi, M ; Garrec, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4881-b61e462ef6ac6434464b10b765434358ace9bf352e53425ffcbc04cced45c0183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Environmental Sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - classification</topic><topic>Listeria monocytogenes - genetics</topic><topic>Listeria monocytogenes - isolation & purification</topic><topic>Listeria monocytogenes - pathogenicity</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Molecular Typing</topic><topic>PFGE subtyping</topic><topic>Serotyping</topic><topic>Sewage - microbiology</topic><topic>sludge</topic><topic>Virulence</topic><topic>virulence assay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kerouanton, A</creatorcontrib><creatorcontrib>Roche, S.M</creatorcontrib><creatorcontrib>Marault, M</creatorcontrib><creatorcontrib>Velge, P</creatorcontrib><creatorcontrib>Pourcher, A.-M</creatorcontrib><creatorcontrib>Brisabois, A</creatorcontrib><creatorcontrib>Federighi, M</creatorcontrib><creatorcontrib>Garrec, N</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Risk Abstracts</collection><collection>Safety Science and Risk</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kerouanton, A</au><au>Roche, S.M</au><au>Marault, M</au><au>Velge, P</au><au>Pourcher, A.-M</au><au>Brisabois, A</au><au>Federighi, M</au><au>Garrec, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of isolates of Listeria monocytogenes from sludge using pulsed-field gel electrophoresis and virulence assays</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2010-04</date><risdate>2010</risdate><volume>108</volume><issue>4</issue><spage>1380</spage><epage>1388</epage><pages>1380-1388</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>To study the diversity and virulence of Listeria monocytogenes isolated from sludge. A total of 60 isolates of L. monocytogenes from sludge were characterized by serotyping, PFGE typing and using in vitro and in vivo virulence assays. The PFGE patterns were compared with those of food and human isolates to determine whether specific group clones are associated with environmental samples. The 60 isolates gave 44 different combined ApaI/AscI PFGE patterns. The PFGE patterns of most isolates were similar or very similar to those of epidemic isolates. The majority (93%) of isolates were found to be virulent by plaque-forming assay and by mouse virulence assay. Our findings suggest that L. monocytogenes strains found in non-sanitized sludge are virulent and represent a potential health hazard. Although no case of listeriosis related to sludge spread onto agricultural land has been reported, particular attention to this pathogen is needed. This is the first study dealing with the characterization of L. monocytogenes isolates from non-sanitized sludge samples by molecular typing methods and in vitro and in vivo virulence assays. Our findings provide relevant information for evaluating the health risks associated with spreading sludge onto agricultural land.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19796096</pmid><doi>10.1111/j.1365-2672.2009.04531.x</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-1108-6490</orcidid><orcidid>https://orcid.org/0000-0001-5770-7443</orcidid><orcidid>https://orcid.org/0000-0003-0452-2060</orcidid><orcidid>https://orcid.org/0000-0002-8201-0194</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2010-04, Vol.108 (4), p.1380-1388 |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Biological and medical sciences Electrophoresis, Gel, Pulsed-Field Environmental Sciences Fundamental and applied biological sciences. Psychology Humans Listeria monocytogenes Listeria monocytogenes - classification Listeria monocytogenes - genetics Listeria monocytogenes - isolation & purification Listeria monocytogenes - pathogenicity Mice Microbiology Molecular Typing PFGE subtyping Serotyping Sewage - microbiology sludge Virulence virulence assay |
| title | Characterization of isolates of Listeria monocytogenes from sludge using pulsed-field gel electrophoresis and virulence assays |
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