Ex Vivo Uranium Decontamination Efficiency on Wounded Skin and In Vitro Skin Toxicity of a Calixarene-Loaded Nanoemulsion
The present work aims at studying the decontamination efficacy of a calixarene-loaded nanoemulsion on two ex vivo wounded skin models mimicking superficial stings or cuts contaminated with uranium, and on a third model using excoriation. The decontaminating formulation was compared with the currentl...
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| Veröffentlicht in: | Journal of pharmaceutical sciences 2015-06, Vol.104 (6), p.2008-2017 |
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| container_end_page | 2017 |
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| container_issue | 6 |
| container_start_page | 2008 |
| container_title | Journal of pharmaceutical sciences |
| container_volume | 104 |
| creator | Grives, Sophie Phan, Guillaume Morat, Guillaume Suhard, David Rebiere, Francois Fattal, Elias |
| description | The present work aims at studying the decontamination efficacy of a calixarene-loaded nanoemulsion on two ex vivo wounded skin models mimicking superficial stings or cuts contaminated with uranium, and on a third model using excoriation. The decontaminating formulation was compared with the currently used radio-decontaminating soapy water (Trait rouge®) treatment. Moreover, to assess skin damage potentially induced by the undiluted nanoemulsion, in vitro toxicity studies were conducted on an in vitro reconstructed human epidermis, coupled with three differenttoxicity tests [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide, lactate dehydrogenase, and interleukin-1-a]. This work demonstrated not only a significant decontamination activity of the calixarene nanoemulsion on wounded skin, ranging from 92% to 94% of the applied uranium solution according to the ex vivo model used, but also the absence of side effects of this promising treatment. |
| doi_str_mv | 10.1002/jps.24431 |
| format | Article |
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The decontaminating formulation was compared with the currently used radio-decontaminating soapy water (Trait rouge®) treatment. Moreover, to assess skin damage potentially induced by the undiluted nanoemulsion, in vitro toxicity studies were conducted on an in vitro reconstructed human epidermis, coupled with three differenttoxicity tests [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide, lactate dehydrogenase, and interleukin-1-a]. 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The decontaminating formulation was compared with the currently used radio-decontaminating soapy water (Trait rouge®) treatment. Moreover, to assess skin damage potentially induced by the undiluted nanoemulsion, in vitro toxicity studies were conducted on an in vitro reconstructed human epidermis, coupled with three differenttoxicity tests [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide, lactate dehydrogenase, and interleukin-1-a]. 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Phan, Guillaume ; Morat, Guillaume ; Suhard, David ; Rebiere, Francois ; Fattal, Elias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6001-9796528a7fffddf36240fc37621201aad81c2cda4eb7eac54ad7a54e88f44e663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Calixarenes - chemistry</topic><topic>Calixarenes - pharmacology</topic><topic>Calixarenes - toxicity</topic><topic>chelation</topic><topic>Decontamination - methods</topic><topic>diffusion</topic><topic>emulsion</topic><topic>Emulsions - chemistry</topic><topic>Emulsions - pharmacology</topic><topic>Emulsions - toxicity</topic><topic>formulation vehicle</topic><topic>Humans</topic><topic>in vitro models</topic><topic>Life Sciences</topic><topic>nanotechnology</topic><topic>Phenols - chemistry</topic><topic>Phenols - pharmacology</topic><topic>Phenols - toxicity</topic><topic>skin</topic><topic>Skin - drug effects</topic><topic>Skin - pathology</topic><topic>Skin - ultrastructure</topic><topic>Swine</topic><topic>toxicity</topic><topic>Uranium - isolation & purification</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grives, Sophie</creatorcontrib><creatorcontrib>Phan, Guillaume</creatorcontrib><creatorcontrib>Morat, Guillaume</creatorcontrib><creatorcontrib>Suhard, David</creatorcontrib><creatorcontrib>Rebiere, Francois</creatorcontrib><creatorcontrib>Fattal, Elias</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grives, Sophie</au><au>Phan, Guillaume</au><au>Morat, Guillaume</au><au>Suhard, David</au><au>Rebiere, Francois</au><au>Fattal, Elias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ex Vivo Uranium Decontamination Efficiency on Wounded Skin and In Vitro Skin Toxicity of a Calixarene-Loaded Nanoemulsion</atitle><jtitle>Journal of pharmaceutical sciences</jtitle><addtitle>J Pharm Sci</addtitle><date>2015-06</date><risdate>2015</risdate><volume>104</volume><issue>6</issue><spage>2008</spage><epage>2017</epage><pages>2008-2017</pages><issn>0022-3549</issn><eissn>1520-6017</eissn><coden>JPMSAE</coden><abstract>The present work aims at studying the decontamination efficacy of a calixarene-loaded nanoemulsion on two ex vivo wounded skin models mimicking superficial stings or cuts contaminated with uranium, and on a third model using excoriation. The decontaminating formulation was compared with the currently used radio-decontaminating soapy water (Trait rouge®) treatment. Moreover, to assess skin damage potentially induced by the undiluted nanoemulsion, in vitro toxicity studies were conducted on an in vitro reconstructed human epidermis, coupled with three differenttoxicity tests [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide, lactate dehydrogenase, and interleukin-1-a]. This work demonstrated not only a significant decontamination activity of the calixarene nanoemulsion on wounded skin, ranging from 92% to 94% of the applied uranium solution according to the ex vivo model used, but also the absence of side effects of this promising treatment.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25846633</pmid><doi>10.1002/jps.24431</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5225-4115</orcidid><orcidid>https://orcid.org/0000-0001-7223-0559</orcidid></addata></record> |
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| ispartof | Journal of pharmaceutical sciences, 2015-06, Vol.104 (6), p.2008-2017 |
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| language | eng |
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| source | MEDLINE; Wiley Journals; Alma/SFX Local Collection |
| subjects | Animals Calixarenes - chemistry Calixarenes - pharmacology Calixarenes - toxicity chelation Decontamination - methods diffusion emulsion Emulsions - chemistry Emulsions - pharmacology Emulsions - toxicity formulation vehicle Humans in vitro models Life Sciences nanotechnology Phenols - chemistry Phenols - pharmacology Phenols - toxicity skin Skin - drug effects Skin - pathology Skin - ultrastructure Swine toxicity Uranium - isolation & purification Wound Healing - drug effects |
| title | Ex Vivo Uranium Decontamination Efficiency on Wounded Skin and In Vitro Skin Toxicity of a Calixarene-Loaded Nanoemulsion |
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