PLGA protein nanocarriers with tailor-made fluorescence/MRI/PET imaging modalities
Designing theranostic nanocarriers with high protein payload and multimodality tracking without cross interferences between the different imaging probes and the delicate protein cargo is challenging. Here, chemical modifications of poly(lactic- co -glycolic acid) (PLGA) to produce nanocapsules (NCs)...
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creator | Zhang, Yajie García-Gabilondo, Miguel Grayston, Alba Feiner, Irene V. J Anton-Sales, Irene Loiola, Rodrigo A Llop, Jordi Ramos-Cabrer, Pedro Barba, Ignasi Garcia-Dorado, David Gosselet, Fabien Rosell, Anna Roig, Anna |
description | Designing theranostic nanocarriers with high protein payload and multimodality tracking without cross interferences between the different imaging probes and the delicate protein cargo is challenging. Here, chemical modifications of poly(lactic-
co
-glycolic acid) (PLGA) to produce nanocapsules (NCs) that incorporate several imaging moieties are reported. The biocompatible and biodegradable PLGA-NCs can be endowed with a magnetic resonance imaging (MRI) reporter, two fluorescence imaging probes (blue/NIR) and a positron emission tomography (PET) reporter. The modular integration of these imaging moieties into the shell of the NCs is successfully achieved without affecting the morphochemical properties of the nanocarrier or the protein loading capacity.
In vivo
biodistribution of the NCs is monitored by MRI, PET and NIRF and the results from different techniques are analyzed comparatively. The viabilities of two different human endothelial cells
in vitro
show no toxicity for NC concentration up to 100 μg mL
−1
. The morbidity of mice for 2 weeks after systemic administration and the hepatic/pancreatic enzymes at the plasma level indicate their
in vivo
biosafety. In summary, the new theranostic PLGA nanoplatform presented here shows versatile
in vitro
/
in vivo
multimodal imaging capabilities, excellent biosafety and over 1 wt% protein loading.
Integrating multimodal imaging modalities in PLGA protein carriers. |
doi_str_mv | 10.1039/c9nr10620k |
format | Article |
fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_02538272v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2364974383</sourcerecordid><originalsourceid>FETCH-LOGICAL-c407t-57b79cef25cb428aa665689777d189a1b785b3e4c51d1e62c1d10b1722a5c05f3</originalsourceid><addsrcrecordid>eNpFkc1PwkAQxTdGI4pevGuaeNKksh_d3fZICAKxKiF4brbbLSyWFndbjf-9i8V6msnMLy9v3gBwheADgiQayKg0CDIM34_AGYYB9Anh-LjrWdAD59ZuIGQRYeQU9AiGlEMGz8BiHk-G3s5UtdKlV4qyksIYrYz1vnS99mqhi8r4W5EpLy-ayigrVSnV4HkxG8zHS09vxUqXK29bZaLQtVb2ApzkorDq8lD74O1xvBxN_fh1MhsNY18GkNc-5SmPpMoxlWmAQyEYoyyMOOcZCiOBUh7SlKhAUpQhxbB0BaaIYyyohDQnfXDX6q5FkeyMM2K-k0roZDqMk_0MYkpCzPEncuxty7pLPxpl62RTNaZ09hLs8ol4QELiqPuWkqay1qi8k0Uw2UedjKKXxW_UTw6-OUg26VZlHfqXrQOuW8BY2W3_f0V-AAWSgO8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2364974383</pqid></control><display><type>article</type><title>PLGA protein nanocarriers with tailor-made fluorescence/MRI/PET imaging modalities</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals 2008-</source><creator>Zhang, Yajie ; García-Gabilondo, Miguel ; Grayston, Alba ; Feiner, Irene V. J ; Anton-Sales, Irene ; Loiola, Rodrigo A ; Llop, Jordi ; Ramos-Cabrer, Pedro ; Barba, Ignasi ; Garcia-Dorado, David ; Gosselet, Fabien ; Rosell, Anna ; Roig, Anna</creator><creatorcontrib>Zhang, Yajie ; García-Gabilondo, Miguel ; Grayston, Alba ; Feiner, Irene V. J ; Anton-Sales, Irene ; Loiola, Rodrigo A ; Llop, Jordi ; Ramos-Cabrer, Pedro ; Barba, Ignasi ; Garcia-Dorado, David ; Gosselet, Fabien ; Rosell, Anna ; Roig, Anna</creatorcontrib><description>Designing theranostic nanocarriers with high protein payload and multimodality tracking without cross interferences between the different imaging probes and the delicate protein cargo is challenging. Here, chemical modifications of poly(lactic-
co
-glycolic acid) (PLGA) to produce nanocapsules (NCs) that incorporate several imaging moieties are reported. The biocompatible and biodegradable PLGA-NCs can be endowed with a magnetic resonance imaging (MRI) reporter, two fluorescence imaging probes (blue/NIR) and a positron emission tomography (PET) reporter. The modular integration of these imaging moieties into the shell of the NCs is successfully achieved without affecting the morphochemical properties of the nanocarrier or the protein loading capacity.
In vivo
biodistribution of the NCs is monitored by MRI, PET and NIRF and the results from different techniques are analyzed comparatively. The viabilities of two different human endothelial cells
in vitro
show no toxicity for NC concentration up to 100 μg mL
−1
. The morbidity of mice for 2 weeks after systemic administration and the hepatic/pancreatic enzymes at the plasma level indicate their
in vivo
biosafety. In summary, the new theranostic PLGA nanoplatform presented here shows versatile
in vitro
/
in vivo
multimodal imaging capabilities, excellent biosafety and over 1 wt% protein loading.
Integrating multimodal imaging modalities in PLGA protein carriers.</description><identifier>ISSN: 2040-3364</identifier><identifier>EISSN: 2040-3372</identifier><identifier>DOI: 10.1039/c9nr10620k</identifier><identifier>PMID: 32057060</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Animals ; Biocompatibility ; Biodegradability ; Cell Line ; Contrast Media - chemistry ; Contrast Media - pharmacology ; Drug Carriers - chemistry ; Drug Carriers - pharmacology ; Endothelial cells ; Female ; Fluorescence ; Glycolic acid ; Humans ; Life Sciences ; Magnetic Resonance Imaging ; Male ; Medical imaging ; Mice ; Mice, Inbred BALB C ; Nanostructures - chemistry ; Nanostructures - therapeutic use ; Optical Imaging ; Organic chemistry ; Polylactic Acid-Polyglycolic Acid Copolymer - chemistry ; Polylactic Acid-Polyglycolic Acid Copolymer - pharmacology ; Positron emission ; Positron-Emission Tomography ; Proteins ; Tomography ; Toxicity</subject><ispartof>Nanoscale, 2020-02, Vol.12 (8), p.4988-52</ispartof><rights>Copyright Royal Society of Chemistry 2020</rights><rights>Attribution - NonCommercial</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-57b79cef25cb428aa665689777d189a1b785b3e4c51d1e62c1d10b1722a5c05f3</citedby><cites>FETCH-LOGICAL-c407t-57b79cef25cb428aa665689777d189a1b785b3e4c51d1e62c1d10b1722a5c05f3</cites><orcidid>0000-0002-0481-5026 ; 0000-0003-3511-2574 ; 0000-0002-0821-9838 ; 0000-0002-1466-0099 ; 0000-0001-6464-7573 ; 0000-0003-1566-1007 ; 0000-0001-7096-1040</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32057060$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://univ-artois.hal.science/hal-02538272$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Yajie</creatorcontrib><creatorcontrib>García-Gabilondo, Miguel</creatorcontrib><creatorcontrib>Grayston, Alba</creatorcontrib><creatorcontrib>Feiner, Irene V. J</creatorcontrib><creatorcontrib>Anton-Sales, Irene</creatorcontrib><creatorcontrib>Loiola, Rodrigo A</creatorcontrib><creatorcontrib>Llop, Jordi</creatorcontrib><creatorcontrib>Ramos-Cabrer, Pedro</creatorcontrib><creatorcontrib>Barba, Ignasi</creatorcontrib><creatorcontrib>Garcia-Dorado, David</creatorcontrib><creatorcontrib>Gosselet, Fabien</creatorcontrib><creatorcontrib>Rosell, Anna</creatorcontrib><creatorcontrib>Roig, Anna</creatorcontrib><title>PLGA protein nanocarriers with tailor-made fluorescence/MRI/PET imaging modalities</title><title>Nanoscale</title><addtitle>Nanoscale</addtitle><description>Designing theranostic nanocarriers with high protein payload and multimodality tracking without cross interferences between the different imaging probes and the delicate protein cargo is challenging. Here, chemical modifications of poly(lactic-
co
-glycolic acid) (PLGA) to produce nanocapsules (NCs) that incorporate several imaging moieties are reported. The biocompatible and biodegradable PLGA-NCs can be endowed with a magnetic resonance imaging (MRI) reporter, two fluorescence imaging probes (blue/NIR) and a positron emission tomography (PET) reporter. The modular integration of these imaging moieties into the shell of the NCs is successfully achieved without affecting the morphochemical properties of the nanocarrier or the protein loading capacity.
In vivo
biodistribution of the NCs is monitored by MRI, PET and NIRF and the results from different techniques are analyzed comparatively. The viabilities of two different human endothelial cells
in vitro
show no toxicity for NC concentration up to 100 μg mL
−1
. The morbidity of mice for 2 weeks after systemic administration and the hepatic/pancreatic enzymes at the plasma level indicate their
in vivo
biosafety. In summary, the new theranostic PLGA nanoplatform presented here shows versatile
in vitro
/
in vivo
multimodal imaging capabilities, excellent biosafety and over 1 wt% protein loading.
Integrating multimodal imaging modalities in PLGA protein carriers.</description><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biodegradability</subject><subject>Cell Line</subject><subject>Contrast Media - chemistry</subject><subject>Contrast Media - pharmacology</subject><subject>Drug Carriers - chemistry</subject><subject>Drug Carriers - pharmacology</subject><subject>Endothelial cells</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Glycolic acid</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Magnetic Resonance Imaging</subject><subject>Male</subject><subject>Medical imaging</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nanostructures - chemistry</subject><subject>Nanostructures - therapeutic use</subject><subject>Optical Imaging</subject><subject>Organic chemistry</subject><subject>Polylactic Acid-Polyglycolic Acid Copolymer - chemistry</subject><subject>Polylactic Acid-Polyglycolic Acid Copolymer - pharmacology</subject><subject>Positron emission</subject><subject>Positron-Emission Tomography</subject><subject>Proteins</subject><subject>Tomography</subject><subject>Toxicity</subject><issn>2040-3364</issn><issn>2040-3372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1PwkAQxTdGI4pevGuaeNKksh_d3fZICAKxKiF4brbbLSyWFndbjf-9i8V6msnMLy9v3gBwheADgiQayKg0CDIM34_AGYYB9Anh-LjrWdAD59ZuIGQRYeQU9AiGlEMGz8BiHk-G3s5UtdKlV4qyksIYrYz1vnS99mqhi8r4W5EpLy-ayigrVSnV4HkxG8zHS09vxUqXK29bZaLQtVb2ApzkorDq8lD74O1xvBxN_fh1MhsNY18GkNc-5SmPpMoxlWmAQyEYoyyMOOcZCiOBUh7SlKhAUpQhxbB0BaaIYyyohDQnfXDX6q5FkeyMM2K-k0roZDqMk_0MYkpCzPEncuxty7pLPxpl62RTNaZ09hLs8ol4QELiqPuWkqay1qi8k0Uw2UedjKKXxW_UTw6-OUg26VZlHfqXrQOuW8BY2W3_f0V-AAWSgO8</recordid><startdate>20200227</startdate><enddate>20200227</enddate><creator>Zhang, Yajie</creator><creator>García-Gabilondo, Miguel</creator><creator>Grayston, Alba</creator><creator>Feiner, Irene V. J</creator><creator>Anton-Sales, Irene</creator><creator>Loiola, Rodrigo A</creator><creator>Llop, Jordi</creator><creator>Ramos-Cabrer, Pedro</creator><creator>Barba, Ignasi</creator><creator>Garcia-Dorado, David</creator><creator>Gosselet, Fabien</creator><creator>Rosell, Anna</creator><creator>Roig, Anna</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-0481-5026</orcidid><orcidid>https://orcid.org/0000-0003-3511-2574</orcidid><orcidid>https://orcid.org/0000-0002-0821-9838</orcidid><orcidid>https://orcid.org/0000-0002-1466-0099</orcidid><orcidid>https://orcid.org/0000-0001-6464-7573</orcidid><orcidid>https://orcid.org/0000-0003-1566-1007</orcidid><orcidid>https://orcid.org/0000-0001-7096-1040</orcidid></search><sort><creationdate>20200227</creationdate><title>PLGA protein nanocarriers with tailor-made fluorescence/MRI/PET imaging modalities</title><author>Zhang, Yajie ; García-Gabilondo, Miguel ; Grayston, Alba ; Feiner, Irene V. J ; Anton-Sales, Irene ; Loiola, Rodrigo A ; Llop, Jordi ; Ramos-Cabrer, Pedro ; Barba, Ignasi ; Garcia-Dorado, David ; Gosselet, Fabien ; Rosell, Anna ; Roig, Anna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-57b79cef25cb428aa665689777d189a1b785b3e4c51d1e62c1d10b1722a5c05f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biodegradability</topic><topic>Cell Line</topic><topic>Contrast Media - chemistry</topic><topic>Contrast Media - pharmacology</topic><topic>Drug Carriers - chemistry</topic><topic>Drug Carriers - pharmacology</topic><topic>Endothelial cells</topic><topic>Female</topic><topic>Fluorescence</topic><topic>Glycolic acid</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Magnetic Resonance Imaging</topic><topic>Male</topic><topic>Medical imaging</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nanostructures - chemistry</topic><topic>Nanostructures - therapeutic use</topic><topic>Optical Imaging</topic><topic>Organic chemistry</topic><topic>Polylactic Acid-Polyglycolic Acid Copolymer - chemistry</topic><topic>Polylactic Acid-Polyglycolic Acid Copolymer - pharmacology</topic><topic>Positron emission</topic><topic>Positron-Emission Tomography</topic><topic>Proteins</topic><topic>Tomography</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yajie</creatorcontrib><creatorcontrib>García-Gabilondo, Miguel</creatorcontrib><creatorcontrib>Grayston, Alba</creatorcontrib><creatorcontrib>Feiner, Irene V. J</creatorcontrib><creatorcontrib>Anton-Sales, Irene</creatorcontrib><creatorcontrib>Loiola, Rodrigo A</creatorcontrib><creatorcontrib>Llop, Jordi</creatorcontrib><creatorcontrib>Ramos-Cabrer, Pedro</creatorcontrib><creatorcontrib>Barba, Ignasi</creatorcontrib><creatorcontrib>Garcia-Dorado, David</creatorcontrib><creatorcontrib>Gosselet, Fabien</creatorcontrib><creatorcontrib>Rosell, Anna</creatorcontrib><creatorcontrib>Roig, Anna</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Nanoscale</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yajie</au><au>García-Gabilondo, Miguel</au><au>Grayston, Alba</au><au>Feiner, Irene V. J</au><au>Anton-Sales, Irene</au><au>Loiola, Rodrigo A</au><au>Llop, Jordi</au><au>Ramos-Cabrer, Pedro</au><au>Barba, Ignasi</au><au>Garcia-Dorado, David</au><au>Gosselet, Fabien</au><au>Rosell, Anna</au><au>Roig, Anna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PLGA protein nanocarriers with tailor-made fluorescence/MRI/PET imaging modalities</atitle><jtitle>Nanoscale</jtitle><addtitle>Nanoscale</addtitle><date>2020-02-27</date><risdate>2020</risdate><volume>12</volume><issue>8</issue><spage>4988</spage><epage>52</epage><pages>4988-52</pages><issn>2040-3364</issn><eissn>2040-3372</eissn><abstract>Designing theranostic nanocarriers with high protein payload and multimodality tracking without cross interferences between the different imaging probes and the delicate protein cargo is challenging. Here, chemical modifications of poly(lactic-
co
-glycolic acid) (PLGA) to produce nanocapsules (NCs) that incorporate several imaging moieties are reported. The biocompatible and biodegradable PLGA-NCs can be endowed with a magnetic resonance imaging (MRI) reporter, two fluorescence imaging probes (blue/NIR) and a positron emission tomography (PET) reporter. The modular integration of these imaging moieties into the shell of the NCs is successfully achieved without affecting the morphochemical properties of the nanocarrier or the protein loading capacity.
In vivo
biodistribution of the NCs is monitored by MRI, PET and NIRF and the results from different techniques are analyzed comparatively. The viabilities of two different human endothelial cells
in vitro
show no toxicity for NC concentration up to 100 μg mL
−1
. The morbidity of mice for 2 weeks after systemic administration and the hepatic/pancreatic enzymes at the plasma level indicate their
in vivo
biosafety. In summary, the new theranostic PLGA nanoplatform presented here shows versatile
in vitro
/
in vivo
multimodal imaging capabilities, excellent biosafety and over 1 wt% protein loading.
Integrating multimodal imaging modalities in PLGA protein carriers.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>32057060</pmid><doi>10.1039/c9nr10620k</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-0481-5026</orcidid><orcidid>https://orcid.org/0000-0003-3511-2574</orcidid><orcidid>https://orcid.org/0000-0002-0821-9838</orcidid><orcidid>https://orcid.org/0000-0002-1466-0099</orcidid><orcidid>https://orcid.org/0000-0001-6464-7573</orcidid><orcidid>https://orcid.org/0000-0003-1566-1007</orcidid><orcidid>https://orcid.org/0000-0001-7096-1040</orcidid><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; Royal Society Of Chemistry Journals 2008- |
subjects | Animals Biocompatibility Biodegradability Cell Line Contrast Media - chemistry Contrast Media - pharmacology Drug Carriers - chemistry Drug Carriers - pharmacology Endothelial cells Female Fluorescence Glycolic acid Humans Life Sciences Magnetic Resonance Imaging Male Medical imaging Mice Mice, Inbred BALB C Nanostructures - chemistry Nanostructures - therapeutic use Optical Imaging Organic chemistry Polylactic Acid-Polyglycolic Acid Copolymer - chemistry Polylactic Acid-Polyglycolic Acid Copolymer - pharmacology Positron emission Positron-Emission Tomography Proteins Tomography Toxicity |
title | PLGA protein nanocarriers with tailor-made fluorescence/MRI/PET imaging modalities |
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