Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas
An expressed sequence tag program was undertaken to isolate genes involved in defense mechanisms of the Pacific oyster, Crassostrea gigas. Putative function could be assigned to 54% of the 1142 sequenced cDNAs. We built a public database where all EST information are accessible through numerous sear...
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| Veröffentlicht in: | Gene 2003-01, Vol.303 (C), p.139-145 |
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| container_title | Gene |
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| creator | Gueguen, Yannick Cadoret, Jean Paul Flament, Didier Barreau-Roumiguière, Catherine Girardot, Anne Laure Garnier, Julien Hoareau, Alixe Bachère, Evelyne Escoubas, Jean Michel |
| description | An expressed sequence tag program was undertaken to isolate genes involved in defense mechanisms of the Pacific oyster, Crassostrea gigas. Putative function could be assigned to 54% of the 1142 sequenced cDNAs. We built a public database where all EST information are accessible through numerous search profiles (http://www.ifremer.fr/GigasBase). Based on sequence similarities we identified 20 genes that may be implicated in immune function. We investigated the expression of four of these genes during bacterial challenge of oysters. Three of them were induced in response to challenge lending support to their involvement in oyster immunity. Moreover, four other genes were highly homologous to components of the NF-kappa B signaling pathway which is involved in innate immune response in Drosophila and mammals. Altogether, our results open a new way to investigate the immune response in mollusks. |
| doi_str_mv | 10.1016/S0378-1119(02)01149-6 |
| format | Article |
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Putative function could be assigned to 54% of the 1142 sequenced cDNAs. We built a public database where all EST information are accessible through numerous search profiles (http://www.ifremer.fr/GigasBase). Based on sequence similarities we identified 20 genes that may be implicated in immune function. We investigated the expression of four of these genes during bacterial challenge of oysters. Three of them were induced in response to challenge lending support to their involvement in oyster immunity. Moreover, four other genes were highly homologous to components of the NF-kappa B signaling pathway which is involved in innate immune response in Drosophila and mammals. 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Putative function could be assigned to 54% of the 1142 sequenced cDNAs. We built a public database where all EST information are accessible through numerous search profiles (http://www.ifremer.fr/GigasBase). Based on sequence similarities we identified 20 genes that may be implicated in immune function. We investigated the expression of four of these genes during bacterial challenge of oysters. Three of them were induced in response to challenge lending support to their involvement in oyster immunity. Moreover, four other genes were highly homologous to components of the NF-kappa B signaling pathway which is involved in innate immune response in Drosophila and mammals. 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Cadoret, Jean Paul ; Flament, Didier ; Barreau-Roumiguière, Catherine ; Girardot, Anne Laure ; Garnier, Julien ; Hoareau, Alixe ; Bachère, Evelyne ; Escoubas, Jean Michel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-297ab9c3b55522e9ca6e30547cf2fb90838b48a2c8e609daf6a049ac513af7003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Bacteria - immunology</topic><topic>Blood Cells - cytology</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Cell Division - genetics</topic><topic>Crassostrea gigas</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>Endopeptidases - genetics</topic><topic>Expressed Sequence Tags</topic><topic>Ferritins - genetics</topic><topic>Gene Expression Regulation - immunology</topic><topic>Hemocytes - metabolism</topic><topic>Immunity, Innate - genetics</topic><topic>Life Sciences</topic><topic>Marine</topic><topic>Metallothionein - genetics</topic><topic>Ostreidae - genetics</topic><topic>Ostreidae - immunology</topic><topic>Sequence Analysis, DNA</topic><topic>Tissue Inhibitor of Metalloproteinases - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gueguen, Yannick</creatorcontrib><creatorcontrib>Cadoret, Jean Paul</creatorcontrib><creatorcontrib>Flament, Didier</creatorcontrib><creatorcontrib>Barreau-Roumiguière, Catherine</creatorcontrib><creatorcontrib>Girardot, Anne Laure</creatorcontrib><creatorcontrib>Garnier, Julien</creatorcontrib><creatorcontrib>Hoareau, Alixe</creatorcontrib><creatorcontrib>Bachère, Evelyne</creatorcontrib><creatorcontrib>Escoubas, Jean Michel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gueguen, Yannick</au><au>Cadoret, Jean Paul</au><au>Flament, Didier</au><au>Barreau-Roumiguière, Catherine</au><au>Girardot, Anne Laure</au><au>Garnier, Julien</au><au>Hoareau, Alixe</au><au>Bachère, Evelyne</au><au>Escoubas, Jean Michel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2003-01-16</date><risdate>2003</risdate><volume>303</volume><issue>C</issue><spage>139</spage><epage>145</epage><pages>139-145</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>An expressed sequence tag program was undertaken to isolate genes involved in defense mechanisms of the Pacific oyster, Crassostrea gigas. 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| ispartof | Gene, 2003-01, Vol.303 (C), p.139-145 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Bacteria - immunology Blood Cells - cytology Cell Adhesion Molecules - genetics Cell Division - genetics Crassostrea gigas DNA, Complementary - chemistry DNA, Complementary - genetics Endopeptidases - genetics Expressed Sequence Tags Ferritins - genetics Gene Expression Regulation - immunology Hemocytes - metabolism Immunity, Innate - genetics Life Sciences Marine Metallothionein - genetics Ostreidae - genetics Ostreidae - immunology Sequence Analysis, DNA Tissue Inhibitor of Metalloproteinases - genetics |
| title | Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas |
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