Use of spin traps to detect superoxide production in living cells by electron paramagnetic resonance (EPR) spectroscopy

•Spin trapping coupled to EPR allows specific detection of O2− in living cells.•A large variety of cyclic nitrone spin traps have been proposed for this purpose.•Different experimental procedures address production by detached or adherent cells.•Here we put together all recent advances, benefits, an...

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Veröffentlicht in:Methods (San Diego, Calif.) Calif.), 2016-10, Vol.109, p.31-43
Hauptverfasser: Abbas, Kahina, Babić, Nikola, Peyrot, Fabienne
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Sprache:eng
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