Validation of a New Liquid Chromatography-Tandem Mass Spectrometry Ion-Trap Technique for the Simultaneous Determination of Thirteen Anticoagulant Rodenticides, Drugs, or Natural Products
The purpose of this study was to develop and validate a liquid chromatography-tandem mass spectrometry method for the identification and quantification of anticoagulant (anti-vitamin K or AVK) compounds, including rodenticides, drugs, and natural products because no published method could be found....
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| Veröffentlicht in: | Journal of analytical toxicology 2010-03, Vol.34 (2), p.95-102 |
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| container_title | Journal of analytical toxicology |
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| creator | Fourel, Isabelle Hugnet, Christophe Goy-Thollot, Isabelle Berny, Philippe |
| description | The purpose of this study was to develop and validate a liquid chromatography-tandem mass spectrometry method for the identification and quantification of anticoagulant (anti-vitamin K or AVK) compounds, including rodenticides, drugs, and natural products because no published method could be found. The proposed method is based on ion-trap technology with electrospray ionization (ESI) and multiple reaction monitoring (MRM) technique. Each AVK is identified by means of its retention time, precursor ion, and two product ions. Plasma samples are extracted by liquid-liquid partition on Toxi-tube B®. The method was validated on dog plasma and gave good results in terms of specificity, linearity, and percent recovery for the 14 AVK tested (warfarin, acenocoumarol, bromadiolone, brodifacoum, chlorophacinone, coumatetralyl, dicoumarol, difenacoum, difethialone, flocoumafen, fluindione, phenindione, and tioclomarol). The limits of detection ranged from 5 to 25 ng/mL. Intraday repeatability was good, but interday repeatability was more variable though still sufficient for our diagnostic purposes. The technique was successfully applied in a series of clinical investigations to demonstrate its applicability in various animal species and gave very high sensitivity and specificity results. |
| doi_str_mv | 10.1093/jat/34.2.95 |
| format | Article |
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The proposed method is based on ion-trap technology with electrospray ionization (ESI) and multiple reaction monitoring (MRM) technique. Each AVK is identified by means of its retention time, precursor ion, and two product ions. Plasma samples are extracted by liquid-liquid partition on Toxi-tube B®. The method was validated on dog plasma and gave good results in terms of specificity, linearity, and percent recovery for the 14 AVK tested (warfarin, acenocoumarol, bromadiolone, brodifacoum, chlorophacinone, coumatetralyl, dicoumarol, difenacoum, difethialone, flocoumafen, fluindione, phenindione, and tioclomarol). The limits of detection ranged from 5 to 25 ng/mL. Intraday repeatability was good, but interday repeatability was more variable though still sufficient for our diagnostic purposes. The technique was successfully applied in a series of clinical investigations to demonstrate its applicability in various animal species and gave very high sensitivity and specificity results.</description><identifier>ISSN: 0146-4760</identifier><identifier>EISSN: 1945-2403</identifier><identifier>DOI: 10.1093/jat/34.2.95</identifier><identifier>PMID: 20223102</identifier><identifier>CODEN: JATOD3</identifier><language>eng</language><publisher>Niles, IL: Oxford University Press</publisher><subject>Analysis ; Analytical chemistry ; Animals ; Anticoagulants - analysis ; Anticoagulants - blood ; Biodiversity and Ecology ; Biological and medical sciences ; Biological Products - analysis ; Biological Products - blood ; Cats ; Chemical Sciences ; Chromatography, Liquid - methods ; Dogs ; Environmental Sciences ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Horses ; Humans ; Life Sciences ; Medical sciences ; Pharmaceutical Preparations - analysis ; Pharmaceutical Preparations - blood ; Pharmacology. Drug treatments ; Phytopathology. Animal pests. Plant and forest protection ; Poisoning - blood ; Poisoning - diagnosis ; Rats ; Rodenticides - analysis ; Rodenticides - blood ; Sensitivity and Specificity ; Sheep ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry - methods ; Toxicology ; Vertebrates</subject><ispartof>Journal of analytical toxicology, 2010-03, Vol.34 (2), p.95-102</ispartof><rights>2010</rights><rights>2015 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-2f98b097da1e40f1eb38619ba0578fe164f2f8f204606afbc6a38286381a44b93</citedby><orcidid>0000-0001-5470-612X ; 0000-0001-5007-3820</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,782,786,887,1586,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22400100$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20223102$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02481569$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Fourel, Isabelle</creatorcontrib><creatorcontrib>Hugnet, Christophe</creatorcontrib><creatorcontrib>Goy-Thollot, Isabelle</creatorcontrib><creatorcontrib>Berny, Philippe</creatorcontrib><title>Validation of a New Liquid Chromatography-Tandem Mass Spectrometry Ion-Trap Technique for the Simultaneous Determination of Thirteen Anticoagulant Rodenticides, Drugs, or Natural Products</title><title>Journal of analytical toxicology</title><addtitle>Journal of Analytical Toxicology</addtitle><addtitle>Journal of Analytical Toxicology</addtitle><description>The purpose of this study was to develop and validate a liquid chromatography-tandem mass spectrometry method for the identification and quantification of anticoagulant (anti-vitamin K or AVK) compounds, including rodenticides, drugs, and natural products because no published method could be found. The proposed method is based on ion-trap technology with electrospray ionization (ESI) and multiple reaction monitoring (MRM) technique. Each AVK is identified by means of its retention time, precursor ion, and two product ions. Plasma samples are extracted by liquid-liquid partition on Toxi-tube B®. The method was validated on dog plasma and gave good results in terms of specificity, linearity, and percent recovery for the 14 AVK tested (warfarin, acenocoumarol, bromadiolone, brodifacoum, chlorophacinone, coumatetralyl, dicoumarol, difenacoum, difethialone, flocoumafen, fluindione, phenindione, and tioclomarol). The limits of detection ranged from 5 to 25 ng/mL. Intraday repeatability was good, but interday repeatability was more variable though still sufficient for our diagnostic purposes. The technique was successfully applied in a series of clinical investigations to demonstrate its applicability in various animal species and gave very high sensitivity and specificity results.</description><subject>Analysis</subject><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Anticoagulants - analysis</subject><subject>Anticoagulants - blood</subject><subject>Biodiversity and Ecology</subject><subject>Biological and medical sciences</subject><subject>Biological Products - analysis</subject><subject>Biological Products - blood</subject><subject>Cats</subject><subject>Chemical Sciences</subject><subject>Chromatography, Liquid - methods</subject><subject>Dogs</subject><subject>Environmental Sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Horses</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Medical sciences</subject><subject>Pharmaceutical Preparations - analysis</subject><subject>Pharmaceutical Preparations - blood</subject><subject>Pharmacology. Drug treatments</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Poisoning - blood</subject><subject>Poisoning - diagnosis</subject><subject>Rats</subject><subject>Rodenticides - analysis</subject><subject>Rodenticides - blood</subject><subject>Sensitivity and Specificity</subject><subject>Sheep</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Toxicology</subject><subject>Vertebrates</subject><issn>0146-4760</issn><issn>1945-2403</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0s2O0zAQAOAIgdiycOKOfAGEIF3_xUmOVQt0UVkQW2C1F2uSOI13k7jYDtBn4-VwlVKOexpp_HnGGk8UPSV4SnDOzm7AnzE-pdM8uRdNSM6TmHLM7kcTTLiIeSrwSfTIuRuMicgEexidUEwpI5hOoj_foNUVeG16ZGoE6EL9Qiv9Y9AVmjfWdODNxsK22cVr6CvVoY_gHLrcqtKHU-XtDp2bPl4Hg9aqbPpwV6HaWOQbhS51N7QeemUGhxbKK9vp_tht3WjrlerRrPe6NLAZWug9-mIqtU_oSrk3aGGHTQih4AX4wUKLPltTDaV3j6MHNbROPTnE0-jru7fr-TJefXp_Pp-t4pLn2Me0zrMC52kFRHFcE1WwTJC8AJykWa2I4DWts5piLrCAuigFsIyGQWUEOC9ydhq9Gus20Mqt1R3YnTSg5XK2kvscpjwjich_kmBfjnZrTRiE87LTrlRtO85ApjwRnAma3i0ZSzHNxb7m61GW1jhnVX18BMFyvwIyrIBkXFKZJ0E_O9Qdik5VR_vvzwN4fgDgSmhrC32p3X8XlgcTjIN7MTozbO_oGI9QO69-HynYWylSliZyeXUd2l99Z-z6g1ywvy_i1xU</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Fourel, Isabelle</creator><creator>Hugnet, Christophe</creator><creator>Goy-Thollot, Isabelle</creator><creator>Berny, Philippe</creator><general>Oxford University Press</general><general>Preston Publications</general><general>Oxford University Press (OUP): Policy F</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-5470-612X</orcidid><orcidid>https://orcid.org/0000-0001-5007-3820</orcidid></search><sort><creationdate>20100301</creationdate><title>Validation of a New Liquid Chromatography-Tandem Mass Spectrometry Ion-Trap Technique for the Simultaneous Determination of Thirteen Anticoagulant Rodenticides, Drugs, or Natural Products</title><author>Fourel, Isabelle ; Hugnet, Christophe ; Goy-Thollot, Isabelle ; Berny, Philippe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-2f98b097da1e40f1eb38619ba0578fe164f2f8f204606afbc6a38286381a44b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analysis</topic><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Anticoagulants - analysis</topic><topic>Anticoagulants - blood</topic><topic>Biodiversity and Ecology</topic><topic>Biological and medical sciences</topic><topic>Biological Products - analysis</topic><topic>Biological Products - blood</topic><topic>Cats</topic><topic>Chemical Sciences</topic><topic>Chromatography, Liquid - methods</topic><topic>Dogs</topic><topic>Environmental Sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Horses</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Medical sciences</topic><topic>Pharmaceutical Preparations - analysis</topic><topic>Pharmaceutical Preparations - blood</topic><topic>Pharmacology. Drug treatments</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Poisoning - blood</topic><topic>Poisoning - diagnosis</topic><topic>Rats</topic><topic>Rodenticides - analysis</topic><topic>Rodenticides - blood</topic><topic>Sensitivity and Specificity</topic><topic>Sheep</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Toxicology</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fourel, Isabelle</creatorcontrib><creatorcontrib>Hugnet, Christophe</creatorcontrib><creatorcontrib>Goy-Thollot, Isabelle</creatorcontrib><creatorcontrib>Berny, Philippe</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of analytical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fourel, Isabelle</au><au>Hugnet, Christophe</au><au>Goy-Thollot, Isabelle</au><au>Berny, Philippe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validation of a New Liquid Chromatography-Tandem Mass Spectrometry Ion-Trap Technique for the Simultaneous Determination of Thirteen Anticoagulant Rodenticides, Drugs, or Natural Products</atitle><jtitle>Journal of analytical toxicology</jtitle><stitle>Journal of Analytical Toxicology</stitle><addtitle>Journal of Analytical Toxicology</addtitle><date>2010-03-01</date><risdate>2010</risdate><volume>34</volume><issue>2</issue><spage>95</spage><epage>102</epage><pages>95-102</pages><issn>0146-4760</issn><eissn>1945-2403</eissn><coden>JATOD3</coden><abstract>The purpose of this study was to develop and validate a liquid chromatography-tandem mass spectrometry method for the identification and quantification of anticoagulant (anti-vitamin K or AVK) compounds, including rodenticides, drugs, and natural products because no published method could be found. The proposed method is based on ion-trap technology with electrospray ionization (ESI) and multiple reaction monitoring (MRM) technique. Each AVK is identified by means of its retention time, precursor ion, and two product ions. Plasma samples are extracted by liquid-liquid partition on Toxi-tube B®. The method was validated on dog plasma and gave good results in terms of specificity, linearity, and percent recovery for the 14 AVK tested (warfarin, acenocoumarol, bromadiolone, brodifacoum, chlorophacinone, coumatetralyl, dicoumarol, difenacoum, difethialone, flocoumafen, fluindione, phenindione, and tioclomarol). The limits of detection ranged from 5 to 25 ng/mL. Intraday repeatability was good, but interday repeatability was more variable though still sufficient for our diagnostic purposes. The technique was successfully applied in a series of clinical investigations to demonstrate its applicability in various animal species and gave very high sensitivity and specificity results.</abstract><cop>Niles, IL</cop><pub>Oxford University Press</pub><pmid>20223102</pmid><doi>10.1093/jat/34.2.95</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5470-612X</orcidid><orcidid>https://orcid.org/0000-0001-5007-3820</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of analytical toxicology, 2010-03, Vol.34 (2), p.95-102 |
| issn | 0146-4760 1945-2403 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Analysis Analytical chemistry Animals Anticoagulants - analysis Anticoagulants - blood Biodiversity and Ecology Biological and medical sciences Biological Products - analysis Biological Products - blood Cats Chemical Sciences Chromatography, Liquid - methods Dogs Environmental Sciences Fundamental and applied biological sciences. Psychology General pharmacology Horses Humans Life Sciences Medical sciences Pharmaceutical Preparations - analysis Pharmaceutical Preparations - blood Pharmacology. Drug treatments Phytopathology. Animal pests. Plant and forest protection Poisoning - blood Poisoning - diagnosis Rats Rodenticides - analysis Rodenticides - blood Sensitivity and Specificity Sheep Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry - methods Toxicology Vertebrates |
| title | Validation of a New Liquid Chromatography-Tandem Mass Spectrometry Ion-Trap Technique for the Simultaneous Determination of Thirteen Anticoagulant Rodenticides, Drugs, or Natural Products |
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