Validation of a New Liquid Chromatography-Tandem Mass Spectrometry Ion-Trap Technique for the Simultaneous Determination of Thirteen Anticoagulant Rodenticides, Drugs, or Natural Products

The purpose of this study was to develop and validate a liquid chromatography-tandem mass spectrometry method for the identification and quantification of anticoagulant (anti-vitamin K or AVK) compounds, including rodenticides, drugs, and natural products because no published method could be found....

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Veröffentlicht in:Journal of analytical toxicology 2010-03, Vol.34 (2), p.95-102
Hauptverfasser: Fourel, Isabelle, Hugnet, Christophe, Goy-Thollot, Isabelle, Berny, Philippe
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container_title Journal of analytical toxicology
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creator Fourel, Isabelle
Hugnet, Christophe
Goy-Thollot, Isabelle
Berny, Philippe
description The purpose of this study was to develop and validate a liquid chromatography-tandem mass spectrometry method for the identification and quantification of anticoagulant (anti-vitamin K or AVK) compounds, including rodenticides, drugs, and natural products because no published method could be found. The proposed method is based on ion-trap technology with electrospray ionization (ESI) and multiple reaction monitoring (MRM) technique. Each AVK is identified by means of its retention time, precursor ion, and two product ions. Plasma samples are extracted by liquid-liquid partition on Toxi-tube B®. The method was validated on dog plasma and gave good results in terms of specificity, linearity, and percent recovery for the 14 AVK tested (warfarin, acenocoumarol, bromadiolone, brodifacoum, chlorophacinone, coumatetralyl, dicoumarol, difenacoum, difethialone, flocoumafen, fluindione, phenindione, and tioclomarol). The limits of detection ranged from 5 to 25 ng/mL. Intraday repeatability was good, but interday repeatability was more variable though still sufficient for our diagnostic purposes. The technique was successfully applied in a series of clinical investigations to demonstrate its applicability in various animal species and gave very high sensitivity and specificity results.
doi_str_mv 10.1093/jat/34.2.95
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The proposed method is based on ion-trap technology with electrospray ionization (ESI) and multiple reaction monitoring (MRM) technique. Each AVK is identified by means of its retention time, precursor ion, and two product ions. Plasma samples are extracted by liquid-liquid partition on Toxi-tube B®. The method was validated on dog plasma and gave good results in terms of specificity, linearity, and percent recovery for the 14 AVK tested (warfarin, acenocoumarol, bromadiolone, brodifacoum, chlorophacinone, coumatetralyl, dicoumarol, difenacoum, difethialone, flocoumafen, fluindione, phenindione, and tioclomarol). The limits of detection ranged from 5 to 25 ng/mL. Intraday repeatability was good, but interday repeatability was more variable though still sufficient for our diagnostic purposes. 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Psychology ; General pharmacology ; Horses ; Humans ; Life Sciences ; Medical sciences ; Pharmaceutical Preparations - analysis ; Pharmaceutical Preparations - blood ; Pharmacology. Drug treatments ; Phytopathology. Animal pests. Plant and forest protection ; Poisoning - blood ; Poisoning - diagnosis ; Rats ; Rodenticides - analysis ; Rodenticides - blood ; Sensitivity and Specificity ; Sheep ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry - methods ; Toxicology ; Vertebrates</subject><ispartof>Journal of analytical toxicology, 2010-03, Vol.34 (2), p.95-102</ispartof><rights>2010</rights><rights>2015 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-2f98b097da1e40f1eb38619ba0578fe164f2f8f204606afbc6a38286381a44b93</citedby><orcidid>0000-0001-5470-612X ; 0000-0001-5007-3820</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,782,786,887,1586,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=22400100$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20223102$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02481569$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Fourel, Isabelle</creatorcontrib><creatorcontrib>Hugnet, Christophe</creatorcontrib><creatorcontrib>Goy-Thollot, Isabelle</creatorcontrib><creatorcontrib>Berny, Philippe</creatorcontrib><title>Validation of a New Liquid Chromatography-Tandem Mass Spectrometry Ion-Trap Technique for the Simultaneous Determination of Thirteen Anticoagulant Rodenticides, Drugs, or Natural Products</title><title>Journal of analytical toxicology</title><addtitle>Journal of Analytical Toxicology</addtitle><addtitle>Journal of Analytical Toxicology</addtitle><description>The purpose of this study was to develop and validate a liquid chromatography-tandem mass spectrometry method for the identification and quantification of anticoagulant (anti-vitamin K or AVK) compounds, including rodenticides, drugs, and natural products because no published method could be found. 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Psychology</subject><subject>General pharmacology</subject><subject>Horses</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Medical sciences</subject><subject>Pharmaceutical Preparations - analysis</subject><subject>Pharmaceutical Preparations - blood</subject><subject>Pharmacology. Drug treatments</subject><subject>Phytopathology. Animal pests. 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source MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Analysis
Analytical chemistry
Animals
Anticoagulants - analysis
Anticoagulants - blood
Biodiversity and Ecology
Biological and medical sciences
Biological Products - analysis
Biological Products - blood
Cats
Chemical Sciences
Chromatography, Liquid - methods
Dogs
Environmental Sciences
Fundamental and applied biological sciences. Psychology
General pharmacology
Horses
Humans
Life Sciences
Medical sciences
Pharmaceutical Preparations - analysis
Pharmaceutical Preparations - blood
Pharmacology. Drug treatments
Phytopathology. Animal pests. Plant and forest protection
Poisoning - blood
Poisoning - diagnosis
Rats
Rodenticides - analysis
Rodenticides - blood
Sensitivity and Specificity
Sheep
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry - methods
Toxicology
Vertebrates
title Validation of a New Liquid Chromatography-Tandem Mass Spectrometry Ion-Trap Technique for the Simultaneous Determination of Thirteen Anticoagulant Rodenticides, Drugs, or Natural Products
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