Parallel acoustic detection of biological warfare agents surrogates by means of piezoelectric immunochips
This paper focuses on flow functionalization of piezoelectric immunochips with antibodies against four different biological warfare agents (BWA) surrogates. To perform parallel detection of all BWA surrogates at once, the E4 Quartz Crystal Microbalance with Dissipation monitoring system (QCM-D) is u...
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| Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2009-05, Vol.138 (2), p.532-538 |
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| container_title | Sensors and actuators. B, Chemical |
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| creator | Alava, Thomas Berthet-Duroure, Nathalie Ayela, Cédric Trévisiol, Emmanuelle Pugnière, Martine Morel, Yannick Rameil, Pascal Nicu, Liviu |
| description | This paper focuses on flow functionalization of piezoelectric immunochips with antibodies against four different biological warfare agents (BWA) surrogates. To perform parallel detection of all BWA surrogates at once, the E4 Quartz Crystal Microbalance with Dissipation monitoring system (QCM-D) is used. Assessment of antibodies immobilization, parallel detection of related BWA surrogates diluted in buffer solutions and regeneration of the complex antibodies/BWA surrogates are first discussed. Minimal detection thresholds for Escherichia coli MRE 162, Bacillus atrophaeus, Cydia pomonella granulosis virus (CpGV) and ovalbumin are respectively equal to 2.4 × 107 CFU/mL, 1.4 × 106 spores/mL, 1.1 × 108 granules/mL and 1 μg/mL.Detection experiments for three of the four BWA surrogates (E. coli MRE 162, B. atrophaeus and ovalbumin) immersed in real liquid matrices from air sampler are successfully performed. |
| doi_str_mv | 10.1016/j.snb.2009.02.060 |
| format | Article |
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| ispartof | Sensors and actuators. B, Chemical, 2009-05, Vol.138 (2), p.532-538 |
| issn | 0925-4005 1873-3077 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Engineering Sciences Micro and nanotechnologies Microelectronics |
| title | Parallel acoustic detection of biological warfare agents surrogates by means of piezoelectric immunochips |
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