Genome-wide RNAi screen of Ca2+ influx identifies genes that regulate Ca2+ release-activated Ca2+ channel activity
Recent studies by our group and others demonstrated a required and conserved role of Stim in store-operated Ca 2+ influx and Ca 2+ release-activated Ca 2+ (CRAC) channel activity. By using an unbiased genome-wide RNA interference screen in Drosophila S2 cells, we now identify 75 hits that strongly i...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2006-06, Vol.103 (24), p.9357-9362 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 9362 |
|---|---|
| container_issue | 24 |
| container_start_page | 9357 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 103 |
| creator | Zhang, S. Yeromin, A. Zhang, X. H.-F. Yu, Y. Safrina, O. Penna, A. Roos, J. Stauderman, K. Cahalan, M. |
| description | Recent studies by our group and others demonstrated a required and conserved role of Stim in store-operated Ca 2+ influx and Ca 2+ release-activated Ca 2+ (CRAC) channel activity. By using an unbiased genome-wide RNA interference screen in Drosophila S2 cells, we now identify 75 hits that strongly inhibited Ca 2+ influx upon store emptying by thapsigargin. Among these hits are 11 predicted transmembrane proteins, including Stim , and one, olf186-F , that upon RNA interference-mediated knockdown exhibited a profound reduction of thapsigargin-evoked Ca 2+ entry and CRAC current, and upon overexpression a 3-fold augmentation of CRAC current. CRAC currents were further increased to 8-fold higher than control and developed more rapidly when olf186-F was cotransfected with Stim . olf186-F is a member of a highly conserved family of four-transmembrane spanning proteins with homologs from Caenorhabditis elegans to human. The endoplasmic reticulum (ER) Ca 2+ pump sarco-/ER calcium ATPase (SERCA) and the single transmembrane-soluble N -ethylmaleimide-sensitive (NSF) attachment receptor (SNARE) protein Syntaxin5 also were required for CRAC channel activity, consistent with a signaling pathway in which Stim senses Ca 2+ depletion within the ER, translocates to the plasma membrane, and interacts with olf186-F to trigger CRAC channel activity. capacitative calcium entry (CCE) genome-wide screen CRAC channel RNA interference store-operated calcium (SOC) influx |
| doi_str_mv | 10.1073/pnas.0603161103 |
| format | Article |
| fullrecord | <record><control><sourceid>hal_pnas_</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_02438063v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>oai_HAL_hal_02438063v1</sourcerecordid><originalsourceid>FETCH-LOGICAL-h231t-5905bfdb709c80c8be3aa9006f03d9a89192ba3bab9698ed9dfdc7813f232ad33</originalsourceid><addsrcrecordid>eNp9kdFrFDEQxoNY7Fl99tE8KmXrJNnNJi_CcdhWOBREn8PsZnIb2csdu7lr-983ekXQB19m4Pt-8w3DMPZGwJWAVn3YJ5yvQIMSWghQz9hCgBWVri08ZwsA2VamlvU5eznPPwHANgZesHOh20ZIbRdsuqG021J1Fz3xb1-Wkc_9RJT4LvAVykseUxgP97zYKccQaeYbSqXmATOfaHMYMdMJnWgknKnCPsdjUf1J7gdMiUb-W4754RU7CzjO9PqpX7Af15--r26r9debz6vluhqkErlqLDRd8F0LtjfQm44UogXQAZS3aKywskPVYWe1NeStD75vjVBBKoleqQv28ZS7P3Rb8n05YMLR7ae4xenB7TC6v50UB7fZHZ2ojdSiLgHvTwHDP2O3y7X7pYGslQGtjqKwb5-WlY_8gctLnKydVU1biHf_J1w4jGOm-6weASu-j4E</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Genome-wide RNAi screen of Ca2+ influx identifies genes that regulate Ca2+ release-activated Ca2+ channel activity</title><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Zhang, S. ; Yeromin, A. ; Zhang, X. H.-F. ; Yu, Y. ; Safrina, O. ; Penna, A. ; Roos, J. ; Stauderman, K. ; Cahalan, M.</creator><creatorcontrib>Zhang, S. ; Yeromin, A. ; Zhang, X. H.-F. ; Yu, Y. ; Safrina, O. ; Penna, A. ; Roos, J. ; Stauderman, K. ; Cahalan, M.</creatorcontrib><description>Recent studies by our group and others demonstrated a required and conserved role of Stim in store-operated Ca 2+ influx and Ca 2+ release-activated Ca 2+ (CRAC) channel activity. By using an unbiased genome-wide RNA interference screen in Drosophila S2 cells, we now identify 75 hits that strongly inhibited Ca 2+ influx upon store emptying by thapsigargin. Among these hits are 11 predicted transmembrane proteins, including Stim , and one, olf186-F , that upon RNA interference-mediated knockdown exhibited a profound reduction of thapsigargin-evoked Ca 2+ entry and CRAC current, and upon overexpression a 3-fold augmentation of CRAC current. CRAC currents were further increased to 8-fold higher than control and developed more rapidly when olf186-F was cotransfected with Stim . olf186-F is a member of a highly conserved family of four-transmembrane spanning proteins with homologs from Caenorhabditis elegans to human. The endoplasmic reticulum (ER) Ca 2+ pump sarco-/ER calcium ATPase (SERCA) and the single transmembrane-soluble N -ethylmaleimide-sensitive (NSF) attachment receptor (SNARE) protein Syntaxin5 also were required for CRAC channel activity, consistent with a signaling pathway in which Stim senses Ca 2+ depletion within the ER, translocates to the plasma membrane, and interacts with olf186-F to trigger CRAC channel activity. capacitative calcium entry (CCE) genome-wide screen CRAC channel RNA interference store-operated calcium (SOC) influx</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0603161103</identifier><identifier>PMID: 16751269</identifier><language>eng</language><publisher>National Acad Sciences</publisher><subject>Biological Sciences ; Cellular Biology ; Life Sciences ; Subcellular Processes</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2006-06, Vol.103 (24), p.9357-9362</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2006 by The National Academy of Sciences of the USA 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-1070-5990 ; 0000-0002-2967-790X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/103/24.cover.gif</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1482614/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1482614/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://hal.science/hal-02438063$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, S.</creatorcontrib><creatorcontrib>Yeromin, A.</creatorcontrib><creatorcontrib>Zhang, X. H.-F.</creatorcontrib><creatorcontrib>Yu, Y.</creatorcontrib><creatorcontrib>Safrina, O.</creatorcontrib><creatorcontrib>Penna, A.</creatorcontrib><creatorcontrib>Roos, J.</creatorcontrib><creatorcontrib>Stauderman, K.</creatorcontrib><creatorcontrib>Cahalan, M.</creatorcontrib><title>Genome-wide RNAi screen of Ca2+ influx identifies genes that regulate Ca2+ release-activated Ca2+ channel activity</title><title>Proceedings of the National Academy of Sciences - PNAS</title><description>Recent studies by our group and others demonstrated a required and conserved role of Stim in store-operated Ca 2+ influx and Ca 2+ release-activated Ca 2+ (CRAC) channel activity. By using an unbiased genome-wide RNA interference screen in Drosophila S2 cells, we now identify 75 hits that strongly inhibited Ca 2+ influx upon store emptying by thapsigargin. Among these hits are 11 predicted transmembrane proteins, including Stim , and one, olf186-F , that upon RNA interference-mediated knockdown exhibited a profound reduction of thapsigargin-evoked Ca 2+ entry and CRAC current, and upon overexpression a 3-fold augmentation of CRAC current. CRAC currents were further increased to 8-fold higher than control and developed more rapidly when olf186-F was cotransfected with Stim . olf186-F is a member of a highly conserved family of four-transmembrane spanning proteins with homologs from Caenorhabditis elegans to human. The endoplasmic reticulum (ER) Ca 2+ pump sarco-/ER calcium ATPase (SERCA) and the single transmembrane-soluble N -ethylmaleimide-sensitive (NSF) attachment receptor (SNARE) protein Syntaxin5 also were required for CRAC channel activity, consistent with a signaling pathway in which Stim senses Ca 2+ depletion within the ER, translocates to the plasma membrane, and interacts with olf186-F to trigger CRAC channel activity. capacitative calcium entry (CCE) genome-wide screen CRAC channel RNA interference store-operated calcium (SOC) influx</description><subject>Biological Sciences</subject><subject>Cellular Biology</subject><subject>Life Sciences</subject><subject>Subcellular Processes</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kdFrFDEQxoNY7Fl99tE8KmXrJNnNJi_CcdhWOBREn8PsZnIb2csdu7lr-983ekXQB19m4Pt-8w3DMPZGwJWAVn3YJ5yvQIMSWghQz9hCgBWVri08ZwsA2VamlvU5eznPPwHANgZesHOh20ZIbRdsuqG021J1Fz3xb1-Wkc_9RJT4LvAVykseUxgP97zYKccQaeYbSqXmATOfaHMYMdMJnWgknKnCPsdjUf1J7gdMiUb-W4754RU7CzjO9PqpX7Af15--r26r9debz6vluhqkErlqLDRd8F0LtjfQm44UogXQAZS3aKywskPVYWe1NeStD75vjVBBKoleqQv28ZS7P3Rb8n05YMLR7ae4xenB7TC6v50UB7fZHZ2ojdSiLgHvTwHDP2O3y7X7pYGslQGtjqKwb5-WlY_8gctLnKydVU1biHf_J1w4jGOm-6weASu-j4E</recordid><startdate>20060613</startdate><enddate>20060613</enddate><creator>Zhang, S.</creator><creator>Yeromin, A.</creator><creator>Zhang, X. H.-F.</creator><creator>Yu, Y.</creator><creator>Safrina, O.</creator><creator>Penna, A.</creator><creator>Roos, J.</creator><creator>Stauderman, K.</creator><creator>Cahalan, M.</creator><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1070-5990</orcidid><orcidid>https://orcid.org/0000-0002-2967-790X</orcidid></search><sort><creationdate>20060613</creationdate><title>Genome-wide RNAi screen of Ca2+ influx identifies genes that regulate Ca2+ release-activated Ca2+ channel activity</title><author>Zhang, S. ; Yeromin, A. ; Zhang, X. H.-F. ; Yu, Y. ; Safrina, O. ; Penna, A. ; Roos, J. ; Stauderman, K. ; Cahalan, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h231t-5905bfdb709c80c8be3aa9006f03d9a89192ba3bab9698ed9dfdc7813f232ad33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological Sciences</topic><topic>Cellular Biology</topic><topic>Life Sciences</topic><topic>Subcellular Processes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, S.</creatorcontrib><creatorcontrib>Yeromin, A.</creatorcontrib><creatorcontrib>Zhang, X. H.-F.</creatorcontrib><creatorcontrib>Yu, Y.</creatorcontrib><creatorcontrib>Safrina, O.</creatorcontrib><creatorcontrib>Penna, A.</creatorcontrib><creatorcontrib>Roos, J.</creatorcontrib><creatorcontrib>Stauderman, K.</creatorcontrib><creatorcontrib>Cahalan, M.</creatorcontrib><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, S.</au><au>Yeromin, A.</au><au>Zhang, X. H.-F.</au><au>Yu, Y.</au><au>Safrina, O.</au><au>Penna, A.</au><au>Roos, J.</au><au>Stauderman, K.</au><au>Cahalan, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-wide RNAi screen of Ca2+ influx identifies genes that regulate Ca2+ release-activated Ca2+ channel activity</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><date>2006-06-13</date><risdate>2006</risdate><volume>103</volume><issue>24</issue><spage>9357</spage><epage>9362</epage><pages>9357-9362</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Recent studies by our group and others demonstrated a required and conserved role of Stim in store-operated Ca 2+ influx and Ca 2+ release-activated Ca 2+ (CRAC) channel activity. By using an unbiased genome-wide RNA interference screen in Drosophila S2 cells, we now identify 75 hits that strongly inhibited Ca 2+ influx upon store emptying by thapsigargin. Among these hits are 11 predicted transmembrane proteins, including Stim , and one, olf186-F , that upon RNA interference-mediated knockdown exhibited a profound reduction of thapsigargin-evoked Ca 2+ entry and CRAC current, and upon overexpression a 3-fold augmentation of CRAC current. CRAC currents were further increased to 8-fold higher than control and developed more rapidly when olf186-F was cotransfected with Stim . olf186-F is a member of a highly conserved family of four-transmembrane spanning proteins with homologs from Caenorhabditis elegans to human. The endoplasmic reticulum (ER) Ca 2+ pump sarco-/ER calcium ATPase (SERCA) and the single transmembrane-soluble N -ethylmaleimide-sensitive (NSF) attachment receptor (SNARE) protein Syntaxin5 also were required for CRAC channel activity, consistent with a signaling pathway in which Stim senses Ca 2+ depletion within the ER, translocates to the plasma membrane, and interacts with olf186-F to trigger CRAC channel activity. capacitative calcium entry (CCE) genome-wide screen CRAC channel RNA interference store-operated calcium (SOC) influx</abstract><pub>National Acad Sciences</pub><pmid>16751269</pmid><doi>10.1073/pnas.0603161103</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-1070-5990</orcidid><orcidid>https://orcid.org/0000-0002-2967-790X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2006-06, Vol.103 (24), p.9357-9362 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02438063v1 |
| source | JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Biological Sciences Cellular Biology Life Sciences Subcellular Processes |
| title | Genome-wide RNAi screen of Ca2+ influx identifies genes that regulate Ca2+ release-activated Ca2+ channel activity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T13%3A28%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-hal_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genome-wide%20RNAi%20screen%20of%20Ca2+%20influx%20identifies%20genes%20that%20regulate%20Ca2+%20release-activated%20Ca2+%20channel%20activity&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Zhang,%20S.&rft.date=2006-06-13&rft.volume=103&rft.issue=24&rft.spage=9357&rft.epage=9362&rft.pages=9357-9362&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.0603161103&rft_dat=%3Chal_pnas_%3Eoai_HAL_hal_02438063v1%3C/hal_pnas_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/16751269&rfr_iscdi=true |