Evaluation of ovarian POMC mRNA through quantitative RT-PCR analysis in Rana esculenta
1 Dipartimento di Scienze Morfologiche e Biochimiche Comparate, Universita' degli Studi di Camerino, 62032 Camerino, Italia; and 2 Laboratory of Cellular and Molecular Endocrinology, European Institute for Peptide Research, Institut National de la Santé et de la Recherche Médicale U413, UA CN...
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| Veröffentlicht in: | American Journal of Physiology: Cell Physiology 2001-05, Vol.280 (5), p.C1038-C1044 |
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| container_title | American Journal of Physiology: Cell Physiology |
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| creator | Nabissi, M Soverchia, L Lihrmann, I Vaudry, H Mosconi, G Polzonetti-Magni, A. M |
| description | 1 Dipartimento di Scienze Morfologiche e Biochimiche
Comparate, Universita' degli Studi di Camerino, 62032 Camerino,
Italia; and 2 Laboratory of Cellular and Molecular
Endocrinology, European Institute for Peptide Research, Institut
National de la Santé et de la Recherche Médicale
U413, UA CNRS, University of Rouen, France
The evaluation of changes
in the expression of specific genes requires accurate measurement of
the corresponding mRNA concentration, especially when the gene is
expressed at a very low level. We previously showed that the
proopiomelanocortin (POMC) gene is expressed in the ovary of the frog
Rana esculenta , and, to evaluate its mRNA content in frog
ovary, we have now developed a sensitive quantitative RT-PCR method.
This study provides evidence for the validation of this method and for
the effects of captivity and hypophysectomy on POMC gene expression in
the ovary of this anuran. Our data indicate that ovarian POMC gene is
involved in short-term captivity stress response and seems not
influenced by pituitary. These results are discussed taking into
account the knowledge of the role played by opioids in stress response;
moreover, a local control of POMC gene expression is also suggested.
frog; ovary; proopiomelanocortin gene expression; captivity; hypophysectomy |
| doi_str_mv | 10.1152/ajpcell.2001.280.5.c1038 |
| format | Article |
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Comparate, Universita' degli Studi di Camerino, 62032 Camerino,
Italia; and 2 Laboratory of Cellular and Molecular
Endocrinology, European Institute for Peptide Research, Institut
National de la Santé et de la Recherche Médicale
U413, UA CNRS, University of Rouen, France
The evaluation of changes
in the expression of specific genes requires accurate measurement of
the corresponding mRNA concentration, especially when the gene is
expressed at a very low level. We previously showed that the
proopiomelanocortin (POMC) gene is expressed in the ovary of the frog
Rana esculenta , and, to evaluate its mRNA content in frog
ovary, we have now developed a sensitive quantitative RT-PCR method.
This study provides evidence for the validation of this method and for
the effects of captivity and hypophysectomy on POMC gene expression in
the ovary of this anuran. Our data indicate that ovarian POMC gene is
involved in short-term captivity stress response and seems not
influenced by pituitary. These results are discussed taking into
account the knowledge of the role played by opioids in stress response;
moreover, a local control of POMC gene expression is also suggested.
frog; ovary; proopiomelanocortin gene expression; captivity; hypophysectomy</description><identifier>ISSN: 0363-6143</identifier><identifier>EISSN: 1522-1563</identifier><identifier>DOI: 10.1152/ajpcell.2001.280.5.c1038</identifier><identifier>PMID: 11287315</identifier><language>eng</language><publisher>United States: American Physiological Society</publisher><subject>Animals ; Base Sequence ; Biochemistry, Molecular Biology ; Cellular Biology ; Female ; Follicle Stimulating Hormone ; Follicle Stimulating Hormone - pharmacology ; In Vitro Techniques ; Life Sciences ; Luteinizing Hormone ; Luteinizing Hormone - pharmacology ; Molecular Sequence Data ; Ovary ; Ovary - metabolism ; Pituitary Gland ; Polymerase Chain Reaction ; Polymerase Chain Reaction - methods ; Pro-Opiomelanocortin ; Pro-Opiomelanocortin - genetics ; Rana catesbeiana ; Rana esculenta ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Sensitivity and Specificity ; Subcellular Processes ; Tissue Extracts ; Tissue Extracts - pharmacology ; Transcription, Genetic ; Transcription, Genetic - drug effects</subject><ispartof>American Journal of Physiology: Cell Physiology, 2001-05, Vol.280 (5), p.C1038-C1044</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-7b7bbae8938d0aa99263dfc9552e3b32d76ad537c11bc5efc9af1f4eca0680043</citedby><cites>FETCH-LOGICAL-c435t-7b7bbae8938d0aa99263dfc9552e3b32d76ad537c11bc5efc9af1f4eca0680043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11287315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://normandie-univ.hal.science/hal-02427184$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Nabissi, M</creatorcontrib><creatorcontrib>Soverchia, L</creatorcontrib><creatorcontrib>Lihrmann, I</creatorcontrib><creatorcontrib>Vaudry, H</creatorcontrib><creatorcontrib>Mosconi, G</creatorcontrib><creatorcontrib>Polzonetti-Magni, A. M</creatorcontrib><title>Evaluation of ovarian POMC mRNA through quantitative RT-PCR analysis in Rana esculenta</title><title>American Journal of Physiology: Cell Physiology</title><addtitle>Am J Physiol Cell Physiol</addtitle><description>1 Dipartimento di Scienze Morfologiche e Biochimiche
Comparate, Universita' degli Studi di Camerino, 62032 Camerino,
Italia; and 2 Laboratory of Cellular and Molecular
Endocrinology, European Institute for Peptide Research, Institut
National de la Santé et de la Recherche Médicale
U413, UA CNRS, University of Rouen, France
The evaluation of changes
in the expression of specific genes requires accurate measurement of
the corresponding mRNA concentration, especially when the gene is
expressed at a very low level. We previously showed that the
proopiomelanocortin (POMC) gene is expressed in the ovary of the frog
Rana esculenta , and, to evaluate its mRNA content in frog
ovary, we have now developed a sensitive quantitative RT-PCR method.
This study provides evidence for the validation of this method and for
the effects of captivity and hypophysectomy on POMC gene expression in
the ovary of this anuran. Our data indicate that ovarian POMC gene is
involved in short-term captivity stress response and seems not
influenced by pituitary. These results are discussed taking into
account the knowledge of the role played by opioids in stress response;
moreover, a local control of POMC gene expression is also suggested.
frog; ovary; proopiomelanocortin gene expression; captivity; hypophysectomy</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biochemistry, Molecular Biology</subject><subject>Cellular Biology</subject><subject>Female</subject><subject>Follicle Stimulating Hormone</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Life Sciences</subject><subject>Luteinizing Hormone</subject><subject>Luteinizing Hormone - pharmacology</subject><subject>Molecular Sequence Data</subject><subject>Ovary</subject><subject>Ovary - metabolism</subject><subject>Pituitary Gland</subject><subject>Polymerase Chain Reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Pro-Opiomelanocortin</subject><subject>Pro-Opiomelanocortin - genetics</subject><subject>Rana catesbeiana</subject><subject>Rana esculenta</subject><subject>Reproducibility of Results</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Sensitivity and Specificity</subject><subject>Subcellular Processes</subject><subject>Tissue Extracts</subject><subject>Tissue Extracts - pharmacology</subject><subject>Transcription, Genetic</subject><subject>Transcription, Genetic - drug effects</subject><issn>0363-6143</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1u1DAUhS0EokPhFZBXSCyS-ieOE3ajqKVIU1qNBrbWTeJMXHniNE4G5u1xNKF0w8qy7znnHn8IYUpiSgW7gse-0tbGjBAas4zEIq4o4dkrtApjFlGR8tdoRXjKo5Qm_AK98_6REJKwNH-LLihlmeRUrNDP6yPYCUbjOuwa7I4wGOjww_1dgQ_b72s8toOb9i1-mqAbzRiUR423u-ih2GLowJ688dh0eBsuWPtqsrob4T1604D1-sNyXqIfN9e74jba3H_9Vqw3UZVwMUaylGUJOst5VhOAPGcpr5sqF4JpXnJWyxRqwWVFaVkJHSbQ0CbRFZA0C7_hl-jzObcFq_rBHGA4KQdG3a43an4jLGGSZsmRBu2ns7Yf3NOk_agOxs8YodNu8kpKwnLJZmF2FlaD837QzXMyJWrmrxb-auavAn8lVDHzD9aPy46pPOj6n3EBHgRflsJm3_4yg1Z9GxA66_YndTNZu9O_x7_5L5JVXzfBHP_f_NzpRZ0_9daoKA</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Nabissi, M</creator><creator>Soverchia, L</creator><creator>Lihrmann, I</creator><creator>Vaudry, H</creator><creator>Mosconi, G</creator><creator>Polzonetti-Magni, A. M</creator><general>American Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20010501</creationdate><title>Evaluation of ovarian POMC mRNA through quantitative RT-PCR analysis in Rana esculenta</title><author>Nabissi, M ; Soverchia, L ; Lihrmann, I ; Vaudry, H ; Mosconi, G ; Polzonetti-Magni, A. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-7b7bbae8938d0aa99263dfc9552e3b32d76ad537c11bc5efc9af1f4eca0680043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biochemistry, Molecular Biology</topic><topic>Cellular Biology</topic><topic>Female</topic><topic>Follicle Stimulating Hormone</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Life Sciences</topic><topic>Luteinizing Hormone</topic><topic>Luteinizing Hormone - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>Ovary</topic><topic>Ovary - metabolism</topic><topic>Pituitary Gland</topic><topic>Polymerase Chain Reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Pro-Opiomelanocortin</topic><topic>Pro-Opiomelanocortin - genetics</topic><topic>Rana catesbeiana</topic><topic>Rana esculenta</topic><topic>Reproducibility of Results</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>Sensitivity and Specificity</topic><topic>Subcellular Processes</topic><topic>Tissue Extracts</topic><topic>Tissue Extracts - pharmacology</topic><topic>Transcription, Genetic</topic><topic>Transcription, Genetic - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nabissi, M</creatorcontrib><creatorcontrib>Soverchia, L</creatorcontrib><creatorcontrib>Lihrmann, I</creatorcontrib><creatorcontrib>Vaudry, H</creatorcontrib><creatorcontrib>Mosconi, G</creatorcontrib><creatorcontrib>Polzonetti-Magni, A. M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nabissi, M</au><au>Soverchia, L</au><au>Lihrmann, I</au><au>Vaudry, H</au><au>Mosconi, G</au><au>Polzonetti-Magni, A. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of ovarian POMC mRNA through quantitative RT-PCR analysis in Rana esculenta</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><addtitle>Am J Physiol Cell Physiol</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>280</volume><issue>5</issue><spage>C1038</spage><epage>C1044</epage><pages>C1038-C1044</pages><issn>0363-6143</issn><eissn>1522-1563</eissn><abstract>1 Dipartimento di Scienze Morfologiche e Biochimiche
Comparate, Universita' degli Studi di Camerino, 62032 Camerino,
Italia; and 2 Laboratory of Cellular and Molecular
Endocrinology, European Institute for Peptide Research, Institut
National de la Santé et de la Recherche Médicale
U413, UA CNRS, University of Rouen, France
The evaluation of changes
in the expression of specific genes requires accurate measurement of
the corresponding mRNA concentration, especially when the gene is
expressed at a very low level. We previously showed that the
proopiomelanocortin (POMC) gene is expressed in the ovary of the frog
Rana esculenta , and, to evaluate its mRNA content in frog
ovary, we have now developed a sensitive quantitative RT-PCR method.
This study provides evidence for the validation of this method and for
the effects of captivity and hypophysectomy on POMC gene expression in
the ovary of this anuran. Our data indicate that ovarian POMC gene is
involved in short-term captivity stress response and seems not
influenced by pituitary. These results are discussed taking into
account the knowledge of the role played by opioids in stress response;
moreover, a local control of POMC gene expression is also suggested.
frog; ovary; proopiomelanocortin gene expression; captivity; hypophysectomy</abstract><cop>United States</cop><pub>American Physiological Society</pub><pmid>11287315</pmid><doi>10.1152/ajpcell.2001.280.5.c1038</doi></addata></record> |
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| identifier | ISSN: 0363-6143 |
| ispartof | American Journal of Physiology: Cell Physiology, 2001-05, Vol.280 (5), p.C1038-C1044 |
| issn | 0363-6143 1522-1563 |
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| recordid | cdi_hal_primary_oai_HAL_hal_02427184v1 |
| source | MEDLINE; American Physiological Society Paid; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Base Sequence Biochemistry, Molecular Biology Cellular Biology Female Follicle Stimulating Hormone Follicle Stimulating Hormone - pharmacology In Vitro Techniques Life Sciences Luteinizing Hormone Luteinizing Hormone - pharmacology Molecular Sequence Data Ovary Ovary - metabolism Pituitary Gland Polymerase Chain Reaction Polymerase Chain Reaction - methods Pro-Opiomelanocortin Pro-Opiomelanocortin - genetics Rana catesbeiana Rana esculenta Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger RNA, Messenger - analysis RNA, Messenger - genetics Sensitivity and Specificity Subcellular Processes Tissue Extracts Tissue Extracts - pharmacology Transcription, Genetic Transcription, Genetic - drug effects |
| title | Evaluation of ovarian POMC mRNA through quantitative RT-PCR analysis in Rana esculenta |
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