One-Atom Substitution Enables Direct and Continuous Monitoring of Histone Deacylase Activity

We developed a one-step direct assay for the determination of histone deacylase (HDAC) activity by substituting the carbonyl oxygen of the acyl moiety with sulfur, resulting in thioacylated lysine side chains. This modification is recognized by class I HDACs with different efficiencies ranging from...

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Veröffentlicht in:	Biochemistry (Easton) 2019-12, Vol.58 (48), p.4777-4789
Hauptverfasser:	Zessin, Matthes, Kutil, Zsófia, Meleshin, Marat, Nováková, Zora, Ghazy, Ehab, Kalbas, Diana, Marek, Martin, Romier, Christophe, Sippl, Wolfgang, Bařinka, Cyril, Schutkowski, Mike
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biochemistry, Molecular Biology Life Sciences
Online-Zugang:	Volltext
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