Isolation of Daudi Cells with Reduced Sensitivity to Interferon. II. On The Mechanisms of Resistance
Laboratoire d'Oncologie Virale, Institut de Recherches Scientifiques sur le Cancer, 94802 Villejuif Cedex and 1 Laboratorie de Biochimie des Protéines, Université de Montpellier, France The mechanism of interferon resistance was studied in two clones of Daudi cells, DIF 2 and DIF 3 , which exhi...
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| Veröffentlicht in: | Journal of general virology 1983-12, Vol.64 (12), p.2649-2653 |
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| creator | Tovey, Michael G Dron, Michel Mogensen, Knud E Lebleu, Bernard Mechti, Nadir Begon-Lours-Guymarho, Jacqueline |
| description | Laboratoire d'Oncologie Virale, Institut de Recherches Scientifiques sur le Cancer, 94802 Villejuif Cedex
and 1 Laboratorie de Biochimie des Protéines, Université de Montpellier, France
The mechanism of interferon resistance was studied in two clones of Daudi cells, DIF 2 and DIF 3 , which exhibit respectively moderate and pronounced resistance to both the antiviral and antiproliferative actions of human interferons- and - . Clones DIF 2 and DIF 3 were found to possess specific high affinity interferon receptors similar to those of parental Daudi cells. However, DIF 2 cells, which have a tetraploid karyotype, had approximately twice as many interferon-binding sites as either DIF 3 or parental Daudi cells. One of the first detectable changes in Daudi cells following interferon treatment is a rapid increase in the intracellular concentration of cyclic GMP. No increase in cyclic GMP was observed in DIF 2 or DIF 3 cells treated with interferon- . However, neither DIF 2 nor DIF 3 cells respond to sodium azide, a non-physiological inducer of cyclic GMP. Interferon treatment was found to induce the production of 2'-5'-oligo-isoadenylate synthetase in DIF 2 and DIF 3 cells in a manner similar to parental Daudi cells, indicating that these cells possess functional interferon receptors. The levels of 2'-5'-oligo-isoadenylate synthetase and 2'-5' A phosphodiesterase activity were similar in all three cell lines, suggesting that the interferon resistance of clones DIF 2 and DIF 3 was not due to a deficiency of pp(A2' p) n A.
Keywords: IFN resistance, IFN receptors, cGMP, 2'-5' A synthetase, Daudi cells
Received 7 April 1983;
accepted 25 August 1983. |
| doi_str_mv | 10.1099/0022-1317-64-12-2649 |
| format | Article |
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and 1 Laboratorie de Biochimie des Protéines, Université de Montpellier, France
The mechanism of interferon resistance was studied in two clones of Daudi cells, DIF 2 and DIF 3 , which exhibit respectively moderate and pronounced resistance to both the antiviral and antiproliferative actions of human interferons- and - . Clones DIF 2 and DIF 3 were found to possess specific high affinity interferon receptors similar to those of parental Daudi cells. However, DIF 2 cells, which have a tetraploid karyotype, had approximately twice as many interferon-binding sites as either DIF 3 or parental Daudi cells. One of the first detectable changes in Daudi cells following interferon treatment is a rapid increase in the intracellular concentration of cyclic GMP. No increase in cyclic GMP was observed in DIF 2 or DIF 3 cells treated with interferon- . However, neither DIF 2 nor DIF 3 cells respond to sodium azide, a non-physiological inducer of cyclic GMP. Interferon treatment was found to induce the production of 2'-5'-oligo-isoadenylate synthetase in DIF 2 and DIF 3 cells in a manner similar to parental Daudi cells, indicating that these cells possess functional interferon receptors. The levels of 2'-5'-oligo-isoadenylate synthetase and 2'-5' A phosphodiesterase activity were similar in all three cell lines, suggesting that the interferon resistance of clones DIF 2 and DIF 3 was not due to a deficiency of pp(A2' p) n A.
Keywords: IFN resistance, IFN receptors, cGMP, 2'-5' A synthetase, Daudi cells
Received 7 April 1983;
accepted 25 August 1983.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-64-12-2649</identifier><identifier>PMID: 6319552</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Azides - pharmacology ; Biological and medical sciences ; Cell Division ; Cell Line ; Cell Separation ; Clone Cells - physiology ; Cyclic GMP - biosynthesis ; Drug Resistance ; Fundamental and applied biological sciences. Psychology ; Humans ; Interferon Type I - metabolism ; Interferon Type I - pharmacology ; Life Sciences ; Microbiology ; Polynucleotide Ligases - metabolism ; Receptors, Cell Surface - metabolism ; Receptors, Interferon ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Sodium Azide ; Virology</subject><ispartof>Journal of general virology, 1983-12, Vol.64 (12), p.2649-2653</ispartof><rights>1984 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3919-c7c8ac9c13f40d0523ede8aced52244d7de1b0b74f6d286858f787509489f3ed3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9540340$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6319552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02361165$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Tovey, Michael G</creatorcontrib><creatorcontrib>Dron, Michel</creatorcontrib><creatorcontrib>Mogensen, Knud E</creatorcontrib><creatorcontrib>Lebleu, Bernard</creatorcontrib><creatorcontrib>Mechti, Nadir</creatorcontrib><creatorcontrib>Begon-Lours-Guymarho, Jacqueline</creatorcontrib><title>Isolation of Daudi Cells with Reduced Sensitivity to Interferon. II. On The Mechanisms of Resistance</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Laboratoire d'Oncologie Virale, Institut de Recherches Scientifiques sur le Cancer, 94802 Villejuif Cedex
and 1 Laboratorie de Biochimie des Protéines, Université de Montpellier, France
The mechanism of interferon resistance was studied in two clones of Daudi cells, DIF 2 and DIF 3 , which exhibit respectively moderate and pronounced resistance to both the antiviral and antiproliferative actions of human interferons- and - . Clones DIF 2 and DIF 3 were found to possess specific high affinity interferon receptors similar to those of parental Daudi cells. However, DIF 2 cells, which have a tetraploid karyotype, had approximately twice as many interferon-binding sites as either DIF 3 or parental Daudi cells. One of the first detectable changes in Daudi cells following interferon treatment is a rapid increase in the intracellular concentration of cyclic GMP. No increase in cyclic GMP was observed in DIF 2 or DIF 3 cells treated with interferon- . However, neither DIF 2 nor DIF 3 cells respond to sodium azide, a non-physiological inducer of cyclic GMP. Interferon treatment was found to induce the production of 2'-5'-oligo-isoadenylate synthetase in DIF 2 and DIF 3 cells in a manner similar to parental Daudi cells, indicating that these cells possess functional interferon receptors. The levels of 2'-5'-oligo-isoadenylate synthetase and 2'-5' A phosphodiesterase activity were similar in all three cell lines, suggesting that the interferon resistance of clones DIF 2 and DIF 3 was not due to a deficiency of pp(A2' p) n A.
Keywords: IFN resistance, IFN receptors, cGMP, 2'-5' A synthetase, Daudi cells
Received 7 April 1983;
accepted 25 August 1983.</description><subject>Azides - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Cell Separation</subject><subject>Clone Cells - physiology</subject><subject>Cyclic GMP - biosynthesis</subject><subject>Drug Resistance</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Interferon Type I - metabolism</subject><subject>Interferon Type I - pharmacology</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Polynucleotide Ligases - metabolism</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Receptors, Interferon</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Sodium Azide</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkcFq3DAQhkVpSLdp3qAFHUqhB28lWbKtY9i0jWFLIE3OQiuNYhXbSiV7Q94-MrtsCwLBzDf_zPyD0EdK1pRI-Y0Qxgpa0rqoeEFZwSou36AV5ZUoWAbeotUJeYfep_SHEMq5qM_ReVVSKQRbIdum0OvJhxEHh6_1bD3eQN8n_OynDt-BnQ1Y_BvG5Ce_99MLngJuxwmigxjGNW7bNb4d8X0H-BeYTo8-DWkRu4Pk06RHAx_QmdN9gsvjf4Eefny_39wU29uf7eZqW5hSUlmY2jTaSENLx4klgpVgIUfACsY4t7UFuiO7mrvKsqZqROPqphZE8ka6zJYX6OtBt9O9eop-0PFFBe3VzdVWLTHCyorSSuxpZr8c2KcY_s6QJjX4ZPLmeoQwJ0XLhtDcN4P8AJoYUorgTsqUqOUQanFZLS6riivK1HKIXPbpqD_vBrCnoqPzOf_5mNfJ6N7FbJRPJ0wKTsr8_q3kH7tnH0E9wjj4PMvOB7X38b-Wr1NUnMQ</recordid><startdate>198312</startdate><enddate>198312</enddate><creator>Tovey, Michael G</creator><creator>Dron, Michel</creator><creator>Mogensen, Knud E</creator><creator>Lebleu, Bernard</creator><creator>Mechti, Nadir</creator><creator>Begon-Lours-Guymarho, Jacqueline</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><general>Microbiology Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>1XC</scope></search><sort><creationdate>198312</creationdate><title>Isolation of Daudi Cells with Reduced Sensitivity to Interferon. II. On The Mechanisms of Resistance</title><author>Tovey, Michael G ; Dron, Michel ; Mogensen, Knud E ; Lebleu, Bernard ; Mechti, Nadir ; Begon-Lours-Guymarho, Jacqueline</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3919-c7c8ac9c13f40d0523ede8aced52244d7de1b0b74f6d286858f787509489f3ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Azides - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>Cell Line</topic><topic>Cell Separation</topic><topic>Clone Cells - physiology</topic><topic>Cyclic GMP - biosynthesis</topic><topic>Drug Resistance</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Interferon Type I - metabolism</topic><topic>Interferon Type I - pharmacology</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Polynucleotide Ligases - metabolism</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Receptors, Interferon</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Sodium Azide</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tovey, Michael G</creatorcontrib><creatorcontrib>Dron, Michel</creatorcontrib><creatorcontrib>Mogensen, Knud E</creatorcontrib><creatorcontrib>Lebleu, Bernard</creatorcontrib><creatorcontrib>Mechti, Nadir</creatorcontrib><creatorcontrib>Begon-Lours-Guymarho, Jacqueline</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tovey, Michael G</au><au>Dron, Michel</au><au>Mogensen, Knud E</au><au>Lebleu, Bernard</au><au>Mechti, Nadir</au><au>Begon-Lours-Guymarho, Jacqueline</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Daudi Cells with Reduced Sensitivity to Interferon. II. On The Mechanisms of Resistance</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1983-12</date><risdate>1983</risdate><volume>64</volume><issue>12</issue><spage>2649</spage><epage>2653</epage><pages>2649-2653</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Laboratoire d'Oncologie Virale, Institut de Recherches Scientifiques sur le Cancer, 94802 Villejuif Cedex
and 1 Laboratorie de Biochimie des Protéines, Université de Montpellier, France
The mechanism of interferon resistance was studied in two clones of Daudi cells, DIF 2 and DIF 3 , which exhibit respectively moderate and pronounced resistance to both the antiviral and antiproliferative actions of human interferons- and - . Clones DIF 2 and DIF 3 were found to possess specific high affinity interferon receptors similar to those of parental Daudi cells. However, DIF 2 cells, which have a tetraploid karyotype, had approximately twice as many interferon-binding sites as either DIF 3 or parental Daudi cells. One of the first detectable changes in Daudi cells following interferon treatment is a rapid increase in the intracellular concentration of cyclic GMP. No increase in cyclic GMP was observed in DIF 2 or DIF 3 cells treated with interferon- . However, neither DIF 2 nor DIF 3 cells respond to sodium azide, a non-physiological inducer of cyclic GMP. Interferon treatment was found to induce the production of 2'-5'-oligo-isoadenylate synthetase in DIF 2 and DIF 3 cells in a manner similar to parental Daudi cells, indicating that these cells possess functional interferon receptors. The levels of 2'-5'-oligo-isoadenylate synthetase and 2'-5' A phosphodiesterase activity were similar in all three cell lines, suggesting that the interferon resistance of clones DIF 2 and DIF 3 was not due to a deficiency of pp(A2' p) n A.
Keywords: IFN resistance, IFN receptors, cGMP, 2'-5' A synthetase, Daudi cells
Received 7 April 1983;
accepted 25 August 1983.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>6319552</pmid><doi>10.1099/0022-1317-64-12-2649</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1983-12, Vol.64 (12), p.2649-2653 |
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| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Azides - pharmacology Biological and medical sciences Cell Division Cell Line Cell Separation Clone Cells - physiology Cyclic GMP - biosynthesis Drug Resistance Fundamental and applied biological sciences. Psychology Humans Interferon Type I - metabolism Interferon Type I - pharmacology Life Sciences Microbiology Polynucleotide Ligases - metabolism Receptors, Cell Surface - metabolism Receptors, Interferon Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Sodium Azide Virology |
| title | Isolation of Daudi Cells with Reduced Sensitivity to Interferon. II. On The Mechanisms of Resistance |
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