Secretagogue and bacteriostatic active fractions derived from a peptic hydro- lysate of alfalfa RuBisCO small purified subunit
For the first time a purification process for small RuBisCO (ribulose‐1,5‐bisphosphate carboxylase/oxygenase) subunit (SRS) was developed from an industrial by‐product of alfalfa, taking advantage of its solubility at low pH. Only one protein strip (14 kDa) was clearly detected in the sodium dodecyl...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2007-02, Vol.87 (3), p.534-540 |
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| container_title | Journal of the science of food and agriculture |
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| creator | Trovaslet, Marie Kapel, Romain Ravallec-Plé, Rozenn Mouni, Fadoua Clarisse, Martine Faille, Christine Dhulster, Pascal Guillochon, Didier Vercaigne-Marko, Dominique |
| description | For the first time a purification process for small RuBisCO (ribulose‐1,5‐bisphosphate carboxylase/oxygenase) subunit (SRS) was developed from an industrial by‐product of alfalfa, taking advantage of its solubility at low pH. Only one protein strip (14 kDa) was clearly detected in the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) profile of the supernatant at pH 2. The recovery of SRS was 48% by this method, with a purity estimated as 98% by densitometry and reverse phase high‐performance liquid chromatography (RP‐HPLC). Moreover, most polyphenolic compounds were discarded, as confirmed by spectrophotometry and RP‐HPLC. SRS hydrolysis was performed for 20 h at 37 °C using pepsin in ammonia/formic acid buffer at pH 3. The hydrolysate was fractionated on a Sephadex G25 column equilibrated with ethanolamine/HCl buffer. Biological activities were found in two fractions. The first fraction showed slight bacteriostatic properties against two pathogenic bacteria, Salmonella arizonae and Shigella sonnei. The second fraction, tested by radioimmunoassay (RIA), presented a secretagogue activity comparable to that of gastrin. Copyright © 2006 Society of Chemical Industry |
| doi_str_mv | 10.1002/jsfa.2754 |
| format | Article |
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Only one protein strip (14 kDa) was clearly detected in the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) profile of the supernatant at pH 2. The recovery of SRS was 48% by this method, with a purity estimated as 98% by densitometry and reverse phase high‐performance liquid chromatography (RP‐HPLC). Moreover, most polyphenolic compounds were discarded, as confirmed by spectrophotometry and RP‐HPLC. SRS hydrolysis was performed for 20 h at 37 °C using pepsin in ammonia/formic acid buffer at pH 3. The hydrolysate was fractionated on a Sephadex G25 column equilibrated with ethanolamine/HCl buffer. Biological activities were found in two fractions. The first fraction showed slight bacteriostatic properties against two pathogenic bacteria, Salmonella arizonae and Shigella sonnei. The second fraction, tested by radioimmunoassay (RIA), presented a secretagogue activity comparable to that of gastrin. Copyright © 2006 Society of Chemical Industry</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.2754</identifier><identifier>CODEN: JSFAAE</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>alfalfa ; Bacteria ; bacteriostatic activity ; Biological and medical sciences ; Chemical and Process Engineering ; Engineering Sciences ; enzymatic hydrolysis ; Food industries ; Fundamental and applied biological sciences. Psychology ; pathogenic bacteria ; Salmonella arizonae ; secretagogue ; Shigella sonnei ; small RuBisCO subunit</subject><ispartof>Journal of the science of food and agriculture, 2007-02, Vol.87 (3), p.534-540</ispartof><rights>Copyright © 2006 Society of Chemical Industry</rights><rights>2007 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3944-9a5232139efb8da762e092a280ad38a258ef349a03de35d9812bf02c21d431ea3</citedby><cites>FETCH-LOGICAL-c3944-9a5232139efb8da762e092a280ad38a258ef349a03de35d9812bf02c21d431ea3</cites><orcidid>0000-0002-8697-5225 ; 0000-0003-0137-8518 ; 0000-0002-7446-5391</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.2754$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.2754$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18466974$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.univ-lorraine.fr/hal-02343248$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Trovaslet, Marie</creatorcontrib><creatorcontrib>Kapel, Romain</creatorcontrib><creatorcontrib>Ravallec-Plé, Rozenn</creatorcontrib><creatorcontrib>Mouni, Fadoua</creatorcontrib><creatorcontrib>Clarisse, Martine</creatorcontrib><creatorcontrib>Faille, Christine</creatorcontrib><creatorcontrib>Dhulster, Pascal</creatorcontrib><creatorcontrib>Guillochon, Didier</creatorcontrib><creatorcontrib>Vercaigne-Marko, Dominique</creatorcontrib><title>Secretagogue and bacteriostatic active fractions derived from a peptic hydro- lysate of alfalfa RuBisCO small purified subunit</title><title>Journal of the science of food and agriculture</title><addtitle>J. Sci. Food Agric</addtitle><description>For the first time a purification process for small RuBisCO (ribulose‐1,5‐bisphosphate carboxylase/oxygenase) subunit (SRS) was developed from an industrial by‐product of alfalfa, taking advantage of its solubility at low pH. Only one protein strip (14 kDa) was clearly detected in the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) profile of the supernatant at pH 2. The recovery of SRS was 48% by this method, with a purity estimated as 98% by densitometry and reverse phase high‐performance liquid chromatography (RP‐HPLC). Moreover, most polyphenolic compounds were discarded, as confirmed by spectrophotometry and RP‐HPLC. SRS hydrolysis was performed for 20 h at 37 °C using pepsin in ammonia/formic acid buffer at pH 3. The hydrolysate was fractionated on a Sephadex G25 column equilibrated with ethanolamine/HCl buffer. Biological activities were found in two fractions. The first fraction showed slight bacteriostatic properties against two pathogenic bacteria, Salmonella arizonae and Shigella sonnei. The second fraction, tested by radioimmunoassay (RIA), presented a secretagogue activity comparable to that of gastrin. Copyright © 2006 Society of Chemical Industry</description><subject>alfalfa</subject><subject>Bacteria</subject><subject>bacteriostatic activity</subject><subject>Biological and medical sciences</subject><subject>Chemical and Process Engineering</subject><subject>Engineering Sciences</subject><subject>enzymatic hydrolysis</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>pathogenic bacteria</subject><subject>Salmonella arizonae</subject><subject>secretagogue</subject><subject>Shigella sonnei</subject><subject>small RuBisCO subunit</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkU9vEzEQxVcIJELhwDfwBSQO247t_edjSElaFLWIBsHNmuyOW5fNerF30-bSz86uErWnqpIle2Z-72nkF0UfORxzAHFyGwweizxNXkUTDiqPATi8jibDTMQpT8Tb6F0ItwCgVJZNoocrKj11eO2ue2LYVGyNZUfeutBhZ0s2VHZLzPjx4ZrAqmG4pWrouA1D1lI7Yje7yruY1buAHTFnGNZmPOxn_9WG2SULG6xr1vbeGjuoQ7_uG9u9j94MWKAPh_so-jX_tpqdxcvLxflsuoxLqZIkVpgKKbhUZNZFhXkmCJRAUQBWskCRFmRkohBkRTKtVMHF2oAoBa8SyQnlUfRl73uDtW693aDfaYdWn02XeuyBkIkUSbHlA_t5z7be_espdHpjQ0l1jQ25PmihUgUiUy-DkPG0UPmLIFeKS8jTpzVL70LwZB535aDHfPWYrx7zHdhPB1MM5fDVHpvShidBkWSZykfuZM_d2Zp2zxvq71fz6cE53its6Oj-UYH-r85ymaf698VCL07nP-D0z0qv5H8B2sPX</recordid><startdate>200702</startdate><enddate>200702</enddate><creator>Trovaslet, Marie</creator><creator>Kapel, Romain</creator><creator>Ravallec-Plé, Rozenn</creator><creator>Mouni, Fadoua</creator><creator>Clarisse, Martine</creator><creator>Faille, Christine</creator><creator>Dhulster, Pascal</creator><creator>Guillochon, Didier</creator><creator>Vercaigne-Marko, Dominique</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>F28</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-8697-5225</orcidid><orcidid>https://orcid.org/0000-0003-0137-8518</orcidid><orcidid>https://orcid.org/0000-0002-7446-5391</orcidid></search><sort><creationdate>200702</creationdate><title>Secretagogue and bacteriostatic active fractions derived from a peptic hydro- lysate of alfalfa RuBisCO small purified subunit</title><author>Trovaslet, Marie ; Kapel, Romain ; Ravallec-Plé, Rozenn ; Mouni, Fadoua ; Clarisse, Martine ; Faille, Christine ; Dhulster, Pascal ; Guillochon, Didier ; Vercaigne-Marko, Dominique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3944-9a5232139efb8da762e092a280ad38a258ef349a03de35d9812bf02c21d431ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>alfalfa</topic><topic>Bacteria</topic><topic>bacteriostatic activity</topic><topic>Biological and medical sciences</topic><topic>Chemical and Process Engineering</topic><topic>Engineering Sciences</topic><topic>enzymatic hydrolysis</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>pathogenic bacteria</topic><topic>Salmonella arizonae</topic><topic>secretagogue</topic><topic>Shigella sonnei</topic><topic>small RuBisCO subunit</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Trovaslet, Marie</creatorcontrib><creatorcontrib>Kapel, Romain</creatorcontrib><creatorcontrib>Ravallec-Plé, Rozenn</creatorcontrib><creatorcontrib>Mouni, Fadoua</creatorcontrib><creatorcontrib>Clarisse, Martine</creatorcontrib><creatorcontrib>Faille, Christine</creatorcontrib><creatorcontrib>Dhulster, Pascal</creatorcontrib><creatorcontrib>Guillochon, Didier</creatorcontrib><creatorcontrib>Vercaigne-Marko, Dominique</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Trovaslet, Marie</au><au>Kapel, Romain</au><au>Ravallec-Plé, Rozenn</au><au>Mouni, Fadoua</au><au>Clarisse, Martine</au><au>Faille, Christine</au><au>Dhulster, Pascal</au><au>Guillochon, Didier</au><au>Vercaigne-Marko, Dominique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Secretagogue and bacteriostatic active fractions derived from a peptic hydro- lysate of alfalfa RuBisCO small purified subunit</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J. Sci. Food Agric</addtitle><date>2007-02</date><risdate>2007</risdate><volume>87</volume><issue>3</issue><spage>534</spage><epage>540</epage><pages>534-540</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><coden>JSFAAE</coden><abstract>For the first time a purification process for small RuBisCO (ribulose‐1,5‐bisphosphate carboxylase/oxygenase) subunit (SRS) was developed from an industrial by‐product of alfalfa, taking advantage of its solubility at low pH. Only one protein strip (14 kDa) was clearly detected in the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) profile of the supernatant at pH 2. The recovery of SRS was 48% by this method, with a purity estimated as 98% by densitometry and reverse phase high‐performance liquid chromatography (RP‐HPLC). Moreover, most polyphenolic compounds were discarded, as confirmed by spectrophotometry and RP‐HPLC. SRS hydrolysis was performed for 20 h at 37 °C using pepsin in ammonia/formic acid buffer at pH 3. 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| ispartof | Journal of the science of food and agriculture, 2007-02, Vol.87 (3), p.534-540 |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | alfalfa Bacteria bacteriostatic activity Biological and medical sciences Chemical and Process Engineering Engineering Sciences enzymatic hydrolysis Food industries Fundamental and applied biological sciences. Psychology pathogenic bacteria Salmonella arizonae secretagogue Shigella sonnei small RuBisCO subunit |
| title | Secretagogue and bacteriostatic active fractions derived from a peptic hydro- lysate of alfalfa RuBisCO small purified subunit |
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