A simple, versatile and efficient method to genetically modify human monocyte-derived dendritic cells with HIV-1–derived lentiviral vectors

Lentiviral vectors derived from the human immunodeficiency type 1 virus (HIV-1 LV) are among the finest tools available today for the genetic modification of human monocyte-derived dendritic cells (MDDCs). However, this process is largely inefficient because MDDCs show a strong resistance to HIV-1 t...

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Veröffentlicht in:	Nature protocols 2011-06, Vol.6 (6), p.806-816
Hauptverfasser:	Berger, Grégory, Durand, Stéphanie, Goujon, Caroline, Nguyen, Xuan-Nhi, Cordeil, Stéphanie, Darlix, Jean-Luc, Cimarelli, Andrea
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2300/1851 631/250/2504/133 631/326/596/2561 Analytical Chemistry Antigens Biological Techniques Biomedical and Life Sciences Cell Culture Techniques Cell Differentiation Computational Biology/Bioinformatics Dendritic cells Dendritic Cells - cytology Dendritic Cells - virology Gene expression Genetic aspects Genetic Engineering - methods Genetic Vectors Genetically modified organisms HIV-1 - genetics Human immunodeficiency virus 1 Humans Immune system Infections Lentivirus Lentivirus - genetics Life Sciences Microarrays Microbiology and Parasitology Monocytes - cytology Organic Chemistry Physiological aspects Polypeptides Proteins Protocol Simian immunodeficiency virus Transduction, Genetic - methods Vectors (Biology) Viral Regulatory and Accessory Proteins - genetics Virion - genetics Virology Viruses
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