Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves
Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis are emerging pathogen parasites in the food domain. However, without standardized methods for their detection in food matrices, parasitic foodborne outbreaks remain neglected. In this study, a new immunomagnetic separation assay (IMS T...
Gespeichert in:
| Veröffentlicht in: | Food microbiology 2016-08, Vol.57, p.36-44 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 44 |
|---|---|
| container_issue | |
| container_start_page | 36 |
| container_title | Food microbiology |
| container_volume | 57 |
| creator | Hohweyer, Jeanne Cazeaux, Catherine Travaillé, Emmanuelle Languet, Emilie Dumètre, Aurélien Aubert, Dominique Terryn, Christine Dubey, Jitender P. Azas, Nadine Houssin, Maryline Loïc, Favennec Villena, Isabelle La Carbona, Stéphanie |
| description | Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis are emerging pathogen parasites in the food domain. However, without standardized methods for their detection in food matrices, parasitic foodborne outbreaks remain neglected. In this study, a new immunomagnetic separation assay (IMS Toxo) targeting the oocyst's wall of T. gondii was developed using a specific purified monoclonal antibody. Performance of this IMS Toxo coupled to microscopic and qPCR analyses was evaluated in terms of limit of detection (LOD) and recovery rate (RR) on: i) simple matrix (LOD = 5 oocysts; RR between 5 and 56%); ii) raspberries and basil (LOD = 33 oocysts/g; RR between 0.2 and 35%). Finally, to simultaneously extract the three protozoa from these food matrices, T. gondii oocysts were directly concentrated (without IMS Toxo) from the supernatant of the IMS of Cryptosporidium and Giardia (oo)cysts. This strategy associated to qPCR detection led to LOD |
| doi_str_mv | 10.1016/j.fm.2016.01.002 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_02317870v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0740002016000034</els_id><sourcerecordid>1779881411</sourcerecordid><originalsourceid>FETCH-LOGICAL-c450t-dce9e732814c96d856cf0f167766aa9dad8f17e234dfda3a6fda5aaa7f0552da3</originalsourceid><addsrcrecordid>eNqNkk1v1DAQhiMEokvhzgn5CBIJ43w54VatoEVaiQPlbE3tsepVEgfbWbX8Cn4yjlJ6QyDLHxo_83o8M1n2mkPBgbcfjoUZizKdCuAFQPkk23Hom7zv--5ptgNRQ57McJa9COEIwHlT9c-zs1JAkybssl_f7LgMESdyS2CaIqlo3cScYfGW2OxddD8dTmxGj8FGCuza3bl5wDDie7b393N0YXbearuMDCfNLi16bZHZiRnnNBsxequS43qZRK1nM_lgQ6RJEUuP3STlgQ2EJwovs2cGh0CvHvbz7PvnT9f7q_zw9fLL_uKQq7qBmGtFPYmq7Hit-lZ3TasMGN4K0baIvUbdGS6orGptNFbYprVBRGGgacpkOc_ebbq3OMjZ2xH9vXRo5dXFQa42KCsuOgEnnti3G5vS8WOhEOVog6Jh2NImeQdptBVv_42KrinrSpTVf6Ci79L_-BoAbKjyLgRP5jFiDnLtBHmUZpRrJ0jgMpU8ubx5UF9uRtKPDn9Kn4CPG0ApyydLXgZl14po61MTSO3s39V_A7L_xRQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1779881411</pqid></control><display><type>article</type><title>Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Hohweyer, Jeanne ; Cazeaux, Catherine ; Travaillé, Emmanuelle ; Languet, Emilie ; Dumètre, Aurélien ; Aubert, Dominique ; Terryn, Christine ; Dubey, Jitender P. ; Azas, Nadine ; Houssin, Maryline ; Loïc, Favennec ; Villena, Isabelle ; La Carbona, Stéphanie</creator><creatorcontrib>Hohweyer, Jeanne ; Cazeaux, Catherine ; Travaillé, Emmanuelle ; Languet, Emilie ; Dumètre, Aurélien ; Aubert, Dominique ; Terryn, Christine ; Dubey, Jitender P. ; Azas, Nadine ; Houssin, Maryline ; Loïc, Favennec ; Villena, Isabelle ; La Carbona, Stéphanie</creatorcontrib><description>Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis are emerging pathogen parasites in the food domain. However, without standardized methods for their detection in food matrices, parasitic foodborne outbreaks remain neglected. In this study, a new immunomagnetic separation assay (IMS Toxo) targeting the oocyst's wall of T. gondii was developed using a specific purified monoclonal antibody. Performance of this IMS Toxo coupled to microscopic and qPCR analyses was evaluated in terms of limit of detection (LOD) and recovery rate (RR) on: i) simple matrix (LOD = 5 oocysts; RR between 5 and 56%); ii) raspberries and basil (LOD = 33 oocysts/g; RR between 0.2 and 35%). Finally, to simultaneously extract the three protozoa from these food matrices, T. gondii oocysts were directly concentrated (without IMS Toxo) from the supernatant of the IMS of Cryptosporidium and Giardia (oo)cysts. This strategy associated to qPCR detection led to LOD <1 to 3 (oo)cysts/g and RR between 2 and 35%. This procedure was coupled to RT-qPCR analyses and showed that the three protozoa persisted on the leaves of basil and remained viable following storage at 4 °C for 8 days. These data strengthen the need to consider these protozoa in food safety.
•A novel direct IMS capture of T. gondii oocysts is presented.•A new method to extract T. gondii, C. parvum, G. duodenalis (oo)cysts from food is proposed.•Recovery rates (2–35%) and limits of detection (≤3 (oo)cysts/g) are defined by qPCR.•Protozoa remain viable on the surface of basil for 8 days at 4 °C.</description><identifier>ISSN: 0740-0020</identifier><identifier>EISSN: 1095-9998</identifier><identifier>DOI: 10.1016/j.fm.2016.01.002</identifier><identifier>PMID: 27052700</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject><![CDATA[Cryptosporidium ; Cryptosporidium - genetics ; Cryptosporidium - growth & development ; Cryptosporidium - isolation & purification ; Food Contamination - analysis ; Foods ; Fruits ; Giardia ; Giardia - genetics ; Giardia - growth & development ; Giardia - isolation & purification ; Giardia intestinalis ; Immunomagnetic separation ; Joining ; Life Sciences ; Microscopy ; Ocimum basilicum ; Ocimum basilicum - parasitology ; Oocysts - growth & development ; Parasites ; Persistence ; Plant Leaves - parasitology ; Protozoa ; Real-time qPCR ; Rubus - parasitology ; Strategy ; Toxoplasma - genetics ; Toxoplasma - growth & development ; Toxoplasma - isolation & purification ; Toxoplasma gondii ; Vegetables ; Viability ; Walls]]></subject><ispartof>Food microbiology, 2016-08, Vol.57, p.36-44</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-dce9e732814c96d856cf0f167766aa9dad8f17e234dfda3a6fda5aaa7f0552da3</citedby><cites>FETCH-LOGICAL-c450t-dce9e732814c96d856cf0f167766aa9dad8f17e234dfda3a6fda5aaa7f0552da3</cites><orcidid>0000-0002-7415-4198</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fm.2016.01.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27052700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://normandie-univ.hal.science/hal-02317870$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Hohweyer, Jeanne</creatorcontrib><creatorcontrib>Cazeaux, Catherine</creatorcontrib><creatorcontrib>Travaillé, Emmanuelle</creatorcontrib><creatorcontrib>Languet, Emilie</creatorcontrib><creatorcontrib>Dumètre, Aurélien</creatorcontrib><creatorcontrib>Aubert, Dominique</creatorcontrib><creatorcontrib>Terryn, Christine</creatorcontrib><creatorcontrib>Dubey, Jitender P.</creatorcontrib><creatorcontrib>Azas, Nadine</creatorcontrib><creatorcontrib>Houssin, Maryline</creatorcontrib><creatorcontrib>Loïc, Favennec</creatorcontrib><creatorcontrib>Villena, Isabelle</creatorcontrib><creatorcontrib>La Carbona, Stéphanie</creatorcontrib><title>Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves</title><title>Food microbiology</title><addtitle>Food Microbiol</addtitle><description>Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis are emerging pathogen parasites in the food domain. However, without standardized methods for their detection in food matrices, parasitic foodborne outbreaks remain neglected. In this study, a new immunomagnetic separation assay (IMS Toxo) targeting the oocyst's wall of T. gondii was developed using a specific purified monoclonal antibody. Performance of this IMS Toxo coupled to microscopic and qPCR analyses was evaluated in terms of limit of detection (LOD) and recovery rate (RR) on: i) simple matrix (LOD = 5 oocysts; RR between 5 and 56%); ii) raspberries and basil (LOD = 33 oocysts/g; RR between 0.2 and 35%). Finally, to simultaneously extract the three protozoa from these food matrices, T. gondii oocysts were directly concentrated (without IMS Toxo) from the supernatant of the IMS of Cryptosporidium and Giardia (oo)cysts. This strategy associated to qPCR detection led to LOD <1 to 3 (oo)cysts/g and RR between 2 and 35%. This procedure was coupled to RT-qPCR analyses and showed that the three protozoa persisted on the leaves of basil and remained viable following storage at 4 °C for 8 days. These data strengthen the need to consider these protozoa in food safety.
•A novel direct IMS capture of T. gondii oocysts is presented.•A new method to extract T. gondii, C. parvum, G. duodenalis (oo)cysts from food is proposed.•Recovery rates (2–35%) and limits of detection (≤3 (oo)cysts/g) are defined by qPCR.•Protozoa remain viable on the surface of basil for 8 days at 4 °C.</description><subject>Cryptosporidium</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium - growth & development</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Food Contamination - analysis</subject><subject>Foods</subject><subject>Fruits</subject><subject>Giardia</subject><subject>Giardia - genetics</subject><subject>Giardia - growth & development</subject><subject>Giardia - isolation & purification</subject><subject>Giardia intestinalis</subject><subject>Immunomagnetic separation</subject><subject>Joining</subject><subject>Life Sciences</subject><subject>Microscopy</subject><subject>Ocimum basilicum</subject><subject>Ocimum basilicum - parasitology</subject><subject>Oocysts - growth & development</subject><subject>Parasites</subject><subject>Persistence</subject><subject>Plant Leaves - parasitology</subject><subject>Protozoa</subject><subject>Real-time qPCR</subject><subject>Rubus - parasitology</subject><subject>Strategy</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma - growth & development</subject><subject>Toxoplasma - isolation & purification</subject><subject>Toxoplasma gondii</subject><subject>Vegetables</subject><subject>Viability</subject><subject>Walls</subject><issn>0740-0020</issn><issn>1095-9998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhiMEokvhzgn5CBIJ43w54VatoEVaiQPlbE3tsepVEgfbWbX8Cn4yjlJ6QyDLHxo_83o8M1n2mkPBgbcfjoUZizKdCuAFQPkk23Hom7zv--5ptgNRQ57McJa9COEIwHlT9c-zs1JAkybssl_f7LgMESdyS2CaIqlo3cScYfGW2OxddD8dTmxGj8FGCuza3bl5wDDie7b393N0YXbearuMDCfNLi16bZHZiRnnNBsxequS43qZRK1nM_lgQ6RJEUuP3STlgQ2EJwovs2cGh0CvHvbz7PvnT9f7q_zw9fLL_uKQq7qBmGtFPYmq7Hit-lZ3TasMGN4K0baIvUbdGS6orGptNFbYprVBRGGgacpkOc_ebbq3OMjZ2xH9vXRo5dXFQa42KCsuOgEnnti3G5vS8WOhEOVog6Jh2NImeQdptBVv_42KrinrSpTVf6Ci79L_-BoAbKjyLgRP5jFiDnLtBHmUZpRrJ0jgMpU8ubx5UF9uRtKPDn9Kn4CPG0ApyydLXgZl14po61MTSO3s39V_A7L_xRQ</recordid><startdate>201608</startdate><enddate>201608</enddate><creator>Hohweyer, Jeanne</creator><creator>Cazeaux, Catherine</creator><creator>Travaillé, Emmanuelle</creator><creator>Languet, Emilie</creator><creator>Dumètre, Aurélien</creator><creator>Aubert, Dominique</creator><creator>Terryn, Christine</creator><creator>Dubey, Jitender P.</creator><creator>Azas, Nadine</creator><creator>Houssin, Maryline</creator><creator>Loïc, Favennec</creator><creator>Villena, Isabelle</creator><creator>La Carbona, Stéphanie</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T2</scope><scope>7T7</scope><scope>7U2</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>F28</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-7415-4198</orcidid></search><sort><creationdate>201608</creationdate><title>Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves</title><author>Hohweyer, Jeanne ; Cazeaux, Catherine ; Travaillé, Emmanuelle ; Languet, Emilie ; Dumètre, Aurélien ; Aubert, Dominique ; Terryn, Christine ; Dubey, Jitender P. ; Azas, Nadine ; Houssin, Maryline ; Loïc, Favennec ; Villena, Isabelle ; La Carbona, Stéphanie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-dce9e732814c96d856cf0f167766aa9dad8f17e234dfda3a6fda5aaa7f0552da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Cryptosporidium</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium - growth & development</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Food Contamination - analysis</topic><topic>Foods</topic><topic>Fruits</topic><topic>Giardia</topic><topic>Giardia - genetics</topic><topic>Giardia - growth & development</topic><topic>Giardia - isolation & purification</topic><topic>Giardia intestinalis</topic><topic>Immunomagnetic separation</topic><topic>Joining</topic><topic>Life Sciences</topic><topic>Microscopy</topic><topic>Ocimum basilicum</topic><topic>Ocimum basilicum - parasitology</topic><topic>Oocysts - growth & development</topic><topic>Parasites</topic><topic>Persistence</topic><topic>Plant Leaves - parasitology</topic><topic>Protozoa</topic><topic>Real-time qPCR</topic><topic>Rubus - parasitology</topic><topic>Strategy</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma - growth & development</topic><topic>Toxoplasma - isolation & purification</topic><topic>Toxoplasma gondii</topic><topic>Vegetables</topic><topic>Viability</topic><topic>Walls</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hohweyer, Jeanne</creatorcontrib><creatorcontrib>Cazeaux, Catherine</creatorcontrib><creatorcontrib>Travaillé, Emmanuelle</creatorcontrib><creatorcontrib>Languet, Emilie</creatorcontrib><creatorcontrib>Dumètre, Aurélien</creatorcontrib><creatorcontrib>Aubert, Dominique</creatorcontrib><creatorcontrib>Terryn, Christine</creatorcontrib><creatorcontrib>Dubey, Jitender P.</creatorcontrib><creatorcontrib>Azas, Nadine</creatorcontrib><creatorcontrib>Houssin, Maryline</creatorcontrib><creatorcontrib>Loïc, Favennec</creatorcontrib><creatorcontrib>Villena, Isabelle</creatorcontrib><creatorcontrib>La Carbona, Stéphanie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Safety Science and Risk</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hohweyer, Jeanne</au><au>Cazeaux, Catherine</au><au>Travaillé, Emmanuelle</au><au>Languet, Emilie</au><au>Dumètre, Aurélien</au><au>Aubert, Dominique</au><au>Terryn, Christine</au><au>Dubey, Jitender P.</au><au>Azas, Nadine</au><au>Houssin, Maryline</au><au>Loïc, Favennec</au><au>Villena, Isabelle</au><au>La Carbona, Stéphanie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves</atitle><jtitle>Food microbiology</jtitle><addtitle>Food Microbiol</addtitle><date>2016-08</date><risdate>2016</risdate><volume>57</volume><spage>36</spage><epage>44</epage><pages>36-44</pages><issn>0740-0020</issn><eissn>1095-9998</eissn><abstract>Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis are emerging pathogen parasites in the food domain. However, without standardized methods for their detection in food matrices, parasitic foodborne outbreaks remain neglected. In this study, a new immunomagnetic separation assay (IMS Toxo) targeting the oocyst's wall of T. gondii was developed using a specific purified monoclonal antibody. Performance of this IMS Toxo coupled to microscopic and qPCR analyses was evaluated in terms of limit of detection (LOD) and recovery rate (RR) on: i) simple matrix (LOD = 5 oocysts; RR between 5 and 56%); ii) raspberries and basil (LOD = 33 oocysts/g; RR between 0.2 and 35%). Finally, to simultaneously extract the three protozoa from these food matrices, T. gondii oocysts were directly concentrated (without IMS Toxo) from the supernatant of the IMS of Cryptosporidium and Giardia (oo)cysts. This strategy associated to qPCR detection led to LOD <1 to 3 (oo)cysts/g and RR between 2 and 35%. This procedure was coupled to RT-qPCR analyses and showed that the three protozoa persisted on the leaves of basil and remained viable following storage at 4 °C for 8 days. These data strengthen the need to consider these protozoa in food safety.
•A novel direct IMS capture of T. gondii oocysts is presented.•A new method to extract T. gondii, C. parvum, G. duodenalis (oo)cysts from food is proposed.•Recovery rates (2–35%) and limits of detection (≤3 (oo)cysts/g) are defined by qPCR.•Protozoa remain viable on the surface of basil for 8 days at 4 °C.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>27052700</pmid><doi>10.1016/j.fm.2016.01.002</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-7415-4198</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0740-0020 |
| ispartof | Food microbiology, 2016-08, Vol.57, p.36-44 |
| issn | 0740-0020 1095-9998 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02317870v1 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Cryptosporidium Cryptosporidium - genetics Cryptosporidium - growth & development Cryptosporidium - isolation & purification Food Contamination - analysis Foods Fruits Giardia Giardia - genetics Giardia - growth & development Giardia - isolation & purification Giardia intestinalis Immunomagnetic separation Joining Life Sciences Microscopy Ocimum basilicum Ocimum basilicum - parasitology Oocysts - growth & development Parasites Persistence Plant Leaves - parasitology Protozoa Real-time qPCR Rubus - parasitology Strategy Toxoplasma - genetics Toxoplasma - growth & development Toxoplasma - isolation & purification Toxoplasma gondii Vegetables Viability Walls |
| title | Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T13%3A56%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Simultaneous%20detection%20of%20the%20protozoan%20parasites%20Toxoplasma,%20Cryptosporidium%20and%20Giardia%20in%20food%20matrices%20and%20their%20persistence%20on%20basil%20leaves&rft.jtitle=Food%20microbiology&rft.au=Hohweyer,%20Jeanne&rft.date=2016-08&rft.volume=57&rft.spage=36&rft.epage=44&rft.pages=36-44&rft.issn=0740-0020&rft.eissn=1095-9998&rft_id=info:doi/10.1016/j.fm.2016.01.002&rft_dat=%3Cproquest_hal_p%3E1779881411%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1779881411&rft_id=info:pmid/27052700&rft_els_id=S0740002016000034&rfr_iscdi=true |