Comparative proteomic study of the venom of the piscivorous cone snail Conus consors
In the context of an exhaustive study of the piscivorous cone snail Conus consors, we performed an in-depth analysis of the intact molecular masses that can be detected in the animal's venom, using MALDI and ESI mass spectrometry. We clearly demonstrated that, for the venom of this species at l...
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| Veröffentlicht in: | Journal of proteomics 2009-03, Vol.72 (2), p.210-218 |
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| creator | Biass, Daniel Dutertre, Sébastien Gerbault, Alain Menou, Jean-Louis Offord, Robin Favreau, Philippe Stöcklin, Reto |
| description | In the context of an exhaustive study of the piscivorous cone snail Conus consors, we performed an in-depth analysis of the intact molecular masses that can be detected in the animal's venom, using MALDI and ESI mass spectrometry. We clearly demonstrated that, for the venom of this species at least, it is essential to use both techniques in order to obtain the broadest data set of molecular masses. Only 20% of the total number of molecules detected were found in both mass lists. The two data sets were also compared in terms of mass range and relative hydrophobicity of the components detected in each. With a view to an extensive analysis of this venom's proteome, we further performed a comparative study by ESI-MS between venom obtained after classical dissection of the venom duct versus venom obtained by milking live animals. Surprisingly, although many fewer components were found in the milked venom than in the dissected venom, ∼50% of those found had not been seen in the dissected venom. Several questions raised by these observations are discussed. With regards to the current knowledge of the cone snail venom composition, our results emphasize the complementary nature of the mass spectrometry methods and of the two techniques used in venom collection. |
| doi_str_mv | 10.1016/j.jprot.2009.01.019 |
| format | Article |
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We clearly demonstrated that, for the venom of this species at least, it is essential to use both techniques in order to obtain the broadest data set of molecular masses. Only 20% of the total number of molecules detected were found in both mass lists. The two data sets were also compared in terms of mass range and relative hydrophobicity of the components detected in each. With a view to an extensive analysis of this venom's proteome, we further performed a comparative study by ESI-MS between venom obtained after classical dissection of the venom duct versus venom obtained by milking live animals. Surprisingly, although many fewer components were found in the milked venom than in the dissected venom, ∼50% of those found had not been seen in the dissected venom. Several questions raised by these observations are discussed. With regards to the current knowledge of the cone snail venom composition, our results emphasize the complementary nature of the mass spectrometry methods and of the two techniques used in venom collection.</description><identifier>ISSN: 1874-3919</identifier><identifier>EISSN: 1876-7737</identifier><identifier>DOI: 10.1016/j.jprot.2009.01.019</identifier><identifier>PMID: 19457347</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Chromatography, High Pressure Liquid - methods ; Computational Biology - methods ; Cone snail ; Conopeptide ; Conotoxin ; Conotoxins - analysis ; Conotoxins - chemistry ; Conus consor ; Conus consors ; Conus Snail ; ESI-MS ; HPLC ; Hydrophobicity ; Life Sciences ; MALDI-TOF-MS ; Marine ; Mass fingerprint ; Mass range ; Mass spectrometry ; Molecular Weight ; Peptide ; Peptides - chemistry ; Peptidomics ; Pharmaceutical sciences ; Proteomics ; Proteomics - methods ; Spectrometry, Mass, Electrospray Ionization - methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Time Factors ; Toxins ; Venom ; Venomics ; Venoms - analysis ; Venoms - chemistry</subject><ispartof>Journal of proteomics, 2009-03, Vol.72 (2), p.210-218</ispartof><rights>2009 Elsevier B.V.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-3aecef3000cfa9cc89a9303c574bd05d20f6150d6b0f6377c72d06681cbb8cc63</citedby><cites>FETCH-LOGICAL-c423t-3aecef3000cfa9cc89a9303c574bd05d20f6150d6b0f6377c72d06681cbb8cc63</cites><orcidid>0000-0002-2945-1484</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1874391909000451$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19457347$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02306883$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Biass, Daniel</creatorcontrib><creatorcontrib>Dutertre, Sébastien</creatorcontrib><creatorcontrib>Gerbault, Alain</creatorcontrib><creatorcontrib>Menou, Jean-Louis</creatorcontrib><creatorcontrib>Offord, Robin</creatorcontrib><creatorcontrib>Favreau, Philippe</creatorcontrib><creatorcontrib>Stöcklin, Reto</creatorcontrib><title>Comparative proteomic study of the venom of the piscivorous cone snail Conus consors</title><title>Journal of proteomics</title><addtitle>J Proteomics</addtitle><description>In the context of an exhaustive study of the piscivorous cone snail Conus consors, we performed an in-depth analysis of the intact molecular masses that can be detected in the animal's venom, using MALDI and ESI mass spectrometry. We clearly demonstrated that, for the venom of this species at least, it is essential to use both techniques in order to obtain the broadest data set of molecular masses. Only 20% of the total number of molecules detected were found in both mass lists. The two data sets were also compared in terms of mass range and relative hydrophobicity of the components detected in each. With a view to an extensive analysis of this venom's proteome, we further performed a comparative study by ESI-MS between venom obtained after classical dissection of the venom duct versus venom obtained by milking live animals. Surprisingly, although many fewer components were found in the milked venom than in the dissected venom, ∼50% of those found had not been seen in the dissected venom. Several questions raised by these observations are discussed. With regards to the current knowledge of the cone snail venom composition, our results emphasize the complementary nature of the mass spectrometry methods and of the two techniques used in venom collection.</description><subject>Animals</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Computational Biology - methods</subject><subject>Cone snail</subject><subject>Conopeptide</subject><subject>Conotoxin</subject><subject>Conotoxins - analysis</subject><subject>Conotoxins - chemistry</subject><subject>Conus consor</subject><subject>Conus consors</subject><subject>Conus Snail</subject><subject>ESI-MS</subject><subject>HPLC</subject><subject>Hydrophobicity</subject><subject>Life Sciences</subject><subject>MALDI-TOF-MS</subject><subject>Marine</subject><subject>Mass fingerprint</subject><subject>Mass range</subject><subject>Mass spectrometry</subject><subject>Molecular Weight</subject><subject>Peptide</subject><subject>Peptides - chemistry</subject><subject>Peptidomics</subject><subject>Pharmaceutical sciences</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Time Factors</subject><subject>Toxins</subject><subject>Venom</subject><subject>Venomics</subject><subject>Venoms - analysis</subject><subject>Venoms - chemistry</subject><issn>1874-3919</issn><issn>1876-7737</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMoun78AkF6Ew9dJ003aQ4eZPELFrys55CmUzZL26xJt7D_3qxd9SYMZDK88w7vQ8g1hSkFyu_X0_XGu36aAcgp0FjyiExoIXgqBBPH332eMknlGTkPYQ3AqZDilJxRmc8Ey8WELOeu3WiveztgsrdD11qThH5b7RJXJ_0KkwE71_58NjYYOzjvtiExrsMkdNo2ydx14yA4Hy7JSa2bgFeH94J8PD8t56_p4v3lbf64SE2esT5lGg3WDABMraUxhdSSATMzkZcVzKoMak5nUPEyNkwII7IKOC-oKcvCGM4uyN3ou9KN2njbar9TTlv1-rhQ-xlkDHhRsIFG7e2ojSE_txh61cYk2DS6w5hFSRCUZ5IXUclGpfEuBI_1rzUFtSev1uqbvNqTV0Bjybh1c_Dfli1WfzsH1FHwMAowEhksehVBYmewsh5Nrypn_z3wBTe-liM</recordid><startdate>20090306</startdate><enddate>20090306</enddate><creator>Biass, Daniel</creator><creator>Dutertre, Sébastien</creator><creator>Gerbault, Alain</creator><creator>Menou, Jean-Louis</creator><creator>Offord, Robin</creator><creator>Favreau, Philippe</creator><creator>Stöcklin, Reto</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-2945-1484</orcidid></search><sort><creationdate>20090306</creationdate><title>Comparative proteomic study of the venom of the piscivorous cone snail Conus consors</title><author>Biass, Daniel ; Dutertre, Sébastien ; Gerbault, Alain ; Menou, Jean-Louis ; Offord, Robin ; Favreau, Philippe ; Stöcklin, Reto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-3aecef3000cfa9cc89a9303c574bd05d20f6150d6b0f6377c72d06681cbb8cc63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Computational Biology - methods</topic><topic>Cone snail</topic><topic>Conopeptide</topic><topic>Conotoxin</topic><topic>Conotoxins - analysis</topic><topic>Conotoxins - chemistry</topic><topic>Conus consor</topic><topic>Conus consors</topic><topic>Conus Snail</topic><topic>ESI-MS</topic><topic>HPLC</topic><topic>Hydrophobicity</topic><topic>Life Sciences</topic><topic>MALDI-TOF-MS</topic><topic>Marine</topic><topic>Mass fingerprint</topic><topic>Mass range</topic><topic>Mass spectrometry</topic><topic>Molecular Weight</topic><topic>Peptide</topic><topic>Peptides - chemistry</topic><topic>Peptidomics</topic><topic>Pharmaceutical sciences</topic><topic>Proteomics</topic><topic>Proteomics - methods</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Time Factors</topic><topic>Toxins</topic><topic>Venom</topic><topic>Venomics</topic><topic>Venoms - analysis</topic><topic>Venoms - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Biass, Daniel</creatorcontrib><creatorcontrib>Dutertre, Sébastien</creatorcontrib><creatorcontrib>Gerbault, Alain</creatorcontrib><creatorcontrib>Menou, Jean-Louis</creatorcontrib><creatorcontrib>Offord, Robin</creatorcontrib><creatorcontrib>Favreau, Philippe</creatorcontrib><creatorcontrib>Stöcklin, Reto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of proteomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Biass, Daniel</au><au>Dutertre, Sébastien</au><au>Gerbault, Alain</au><au>Menou, Jean-Louis</au><au>Offord, Robin</au><au>Favreau, Philippe</au><au>Stöcklin, Reto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative proteomic study of the venom of the piscivorous cone snail Conus consors</atitle><jtitle>Journal of proteomics</jtitle><addtitle>J Proteomics</addtitle><date>2009-03-06</date><risdate>2009</risdate><volume>72</volume><issue>2</issue><spage>210</spage><epage>218</epage><pages>210-218</pages><issn>1874-3919</issn><eissn>1876-7737</eissn><abstract>In the context of an exhaustive study of the piscivorous cone snail Conus consors, we performed an in-depth analysis of the intact molecular masses that can be detected in the animal's venom, using MALDI and ESI mass spectrometry. We clearly demonstrated that, for the venom of this species at least, it is essential to use both techniques in order to obtain the broadest data set of molecular masses. Only 20% of the total number of molecules detected were found in both mass lists. The two data sets were also compared in terms of mass range and relative hydrophobicity of the components detected in each. With a view to an extensive analysis of this venom's proteome, we further performed a comparative study by ESI-MS between venom obtained after classical dissection of the venom duct versus venom obtained by milking live animals. Surprisingly, although many fewer components were found in the milked venom than in the dissected venom, ∼50% of those found had not been seen in the dissected venom. Several questions raised by these observations are discussed. 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| ispartof | Journal of proteomics, 2009-03, Vol.72 (2), p.210-218 |
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| subjects | Animals Chromatography, High Pressure Liquid - methods Computational Biology - methods Cone snail Conopeptide Conotoxin Conotoxins - analysis Conotoxins - chemistry Conus consor Conus consors Conus Snail ESI-MS HPLC Hydrophobicity Life Sciences MALDI-TOF-MS Marine Mass fingerprint Mass range Mass spectrometry Molecular Weight Peptide Peptides - chemistry Peptidomics Pharmaceutical sciences Proteomics Proteomics - methods Spectrometry, Mass, Electrospray Ionization - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Time Factors Toxins Venom Venomics Venoms - analysis Venoms - chemistry |
| title | Comparative proteomic study of the venom of the piscivorous cone snail Conus consors |
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