Aerosol deposition in the lungs of spontaneously breathing rats using Gd-DOTA-based contrast agents and ultra-short echo time MRI at 1.5 Tesla
Purpose Aerosol toxicology and drug delivery through the lungs, which depend on various parameters, require methods to quantify particle deposition. Intrapulmonary‐administered MRI contrast agent combined with lung‐specific imaging sequences has been proposed as a high performance technique for aero...
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| Veröffentlicht in: | Magnetic resonance in medicine 2016-02, Vol.75 (2), p.594-605 |
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| creator | Wang, Hongchen Sebrié, Catherine Ruaud, Jean-Pierre Guillot, Geneviève Bouazizi-Verdier, Khaoula Willoquet, Georges Maître, Xavier Darrasse, Luc de Rochefort, Ludovic |
| description | Purpose
Aerosol toxicology and drug delivery through the lungs, which depend on various parameters, require methods to quantify particle deposition. Intrapulmonary‐administered MRI contrast agent combined with lung‐specific imaging sequences has been proposed as a high performance technique for aerosol research. Here, aerosol deposition is assessed using ultra‐short echo (UTE) sequences.
Methods
Before and after administration of Gd‐DOTA‐based aerosol delivered nose‐only in free‐breathing healthy rats, a T1‐weighted 3D UTE sequence was applied in a clinical 1.5 Tesla scanner. Administration lasted 14 min, and the experiment was performed on six rats. A contrast‐enhanced quantitative analysis was done.
Results
Fifty percent signal enhancement was obtained in the lung parenchyma. Lung clearance of the contrast agent was evaluated to be 14% per h (corresponding to a characteristic clearance time of 3.6 h) and aerosol deposition was shown to be homogeneous throughout the lung in healthy rats. The total deposited dose was estimated to be 1.05 µmol/kg body weight, and the concentration precision was 0.02 mM.
Conclusion
The UTE protocol with nebulized Gd‐DOTA is replicable to significantly enhance the lung parenchyma and to map aerosol deposition. This functional strategy, applied in a clinical system with a clinical nebulization setup and a low inhaled dose, suggests a feasible translation to human. Magn Reson Med 75:594–605, 2016. © 2015 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/mrm.25617 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_02299786v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3923832051</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5877-6b0dc38ccfec28b8010f067daf7c5a5261a7ecc0fbe7799d0d55d138af16c5a73</originalsourceid><addsrcrecordid>eNp90UFv0zAUB_AIgVg3OPAFkCUu7JDOduI4PnYD2kHLpKkIbpbjvLQZTlxsB-iX4DPjkq0HJDjZsn7v6fn9k-QFwVOCMb3oXDelrCD8UTIhjNKUMpE_TiaY5zjNiMhPklPv7zDGQvD8aXJCWYlFnueT5NcMnPXWoBp21rehtT1qexS2gMzQbzyyDfI72wfVgx282aPKgQrbtt8gp4JHgz9c53X65mY9SyvloUY6eqd8QGoDfTSqr9Fg4lPqt9YFBHprUWg7QKvba6QCIlOG1uCNepY8aZTx8Pz-PEs-vXu7vlqky5v59dVsmWpWcp4WFa51VmrdgKZlVWKCG1zwWjVcM8VoQRQHrXFTAedC1LhmrCZZqRpSRMCzs-R87LtVRu5c2ym3l1a1cjFbysMbpjTuqiy-k2hfj3bn7LcBfJBd6zUYM65EEs6LgpWU40hf_UXv7OD6-BNJGRaUZoRk_1OEM85EcWDHEXUMyDtojnMSLA-xyxi7_BN7tC_vOw5VB_VRPuQcwcUIfrQG9v_uJFe3q4eW6VjR-gA_jxXKfZUFzziTnz_O5eXqC38vyEJ-yH4DD9rEsw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1757596311</pqid></control><display><type>article</type><title>Aerosol deposition in the lungs of spontaneously breathing rats using Gd-DOTA-based contrast agents and ultra-short echo time MRI at 1.5 Tesla</title><source>MEDLINE</source><source>Wiley Online Library Free Content</source><source>Access via Wiley Online Library</source><creator>Wang, Hongchen ; Sebrié, Catherine ; Ruaud, Jean-Pierre ; Guillot, Geneviève ; Bouazizi-Verdier, Khaoula ; Willoquet, Georges ; Maître, Xavier ; Darrasse, Luc ; de Rochefort, Ludovic</creator><creatorcontrib>Wang, Hongchen ; Sebrié, Catherine ; Ruaud, Jean-Pierre ; Guillot, Geneviève ; Bouazizi-Verdier, Khaoula ; Willoquet, Georges ; Maître, Xavier ; Darrasse, Luc ; de Rochefort, Ludovic</creatorcontrib><description>Purpose
Aerosol toxicology and drug delivery through the lungs, which depend on various parameters, require methods to quantify particle deposition. Intrapulmonary‐administered MRI contrast agent combined with lung‐specific imaging sequences has been proposed as a high performance technique for aerosol research. Here, aerosol deposition is assessed using ultra‐short echo (UTE) sequences.
Methods
Before and after administration of Gd‐DOTA‐based aerosol delivered nose‐only in free‐breathing healthy rats, a T1‐weighted 3D UTE sequence was applied in a clinical 1.5 Tesla scanner. Administration lasted 14 min, and the experiment was performed on six rats. A contrast‐enhanced quantitative analysis was done.
Results
Fifty percent signal enhancement was obtained in the lung parenchyma. Lung clearance of the contrast agent was evaluated to be 14% per h (corresponding to a characteristic clearance time of 3.6 h) and aerosol deposition was shown to be homogeneous throughout the lung in healthy rats. The total deposited dose was estimated to be 1.05 µmol/kg body weight, and the concentration precision was 0.02 mM.
Conclusion
The UTE protocol with nebulized Gd‐DOTA is replicable to significantly enhance the lung parenchyma and to map aerosol deposition. This functional strategy, applied in a clinical system with a clinical nebulization setup and a low inhaled dose, suggests a feasible translation to human. Magn Reson Med 75:594–605, 2016. © 2015 Wiley Periodicals, Inc.</description><identifier>ISSN: 0740-3194</identifier><identifier>EISSN: 1522-2594</identifier><identifier>DOI: 10.1002/mrm.25617</identifier><identifier>PMID: 25809444</identifier><identifier>CODEN: MRMEEN</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Administration, Inhalation ; Administration, Intranasal ; aerosol deposition ; Aerosol research ; Aerosols ; Animals ; Body weight ; Breathing ; Contrast agents ; contrast enhancement ; Contrast media ; Contrast Media - administration & dosage ; Drug delivery ; Feasibility Studies ; Gadolinium ; Gd-DOTA ; Heterocyclic Compounds - administration & dosage ; Image Processing, Computer-Assisted - methods ; Life Sciences ; Lung - anatomy & histology ; lung MRI ; Lungs ; Magnetic resonance imaging ; Magnetic Resonance Imaging, Cine - methods ; Male ; nebulization ; Organometallic Compounds - administration & dosage ; Parenchyma ; Particle deposition ; Physics ; Rats ; Rats, Wistar ; Respiration ; Toxicology ; ultra-short echo</subject><ispartof>Magnetic resonance in medicine, 2016-02, Vol.75 (2), p.594-605</ispartof><rights>2015 Wiley Periodicals, Inc.</rights><rights>2016 Wiley Periodicals, Inc.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5877-6b0dc38ccfec28b8010f067daf7c5a5261a7ecc0fbe7799d0d55d138af16c5a73</citedby><cites>FETCH-LOGICAL-c5877-6b0dc38ccfec28b8010f067daf7c5a5261a7ecc0fbe7799d0d55d138af16c5a73</cites><orcidid>0000-0001-7466-6452</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrm.25617$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrm.25617$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25809444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02299786$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Hongchen</creatorcontrib><creatorcontrib>Sebrié, Catherine</creatorcontrib><creatorcontrib>Ruaud, Jean-Pierre</creatorcontrib><creatorcontrib>Guillot, Geneviève</creatorcontrib><creatorcontrib>Bouazizi-Verdier, Khaoula</creatorcontrib><creatorcontrib>Willoquet, Georges</creatorcontrib><creatorcontrib>Maître, Xavier</creatorcontrib><creatorcontrib>Darrasse, Luc</creatorcontrib><creatorcontrib>de Rochefort, Ludovic</creatorcontrib><title>Aerosol deposition in the lungs of spontaneously breathing rats using Gd-DOTA-based contrast agents and ultra-short echo time MRI at 1.5 Tesla</title><title>Magnetic resonance in medicine</title><addtitle>Magn. Reson. Med</addtitle><description>Purpose
Aerosol toxicology and drug delivery through the lungs, which depend on various parameters, require methods to quantify particle deposition. Intrapulmonary‐administered MRI contrast agent combined with lung‐specific imaging sequences has been proposed as a high performance technique for aerosol research. Here, aerosol deposition is assessed using ultra‐short echo (UTE) sequences.
Methods
Before and after administration of Gd‐DOTA‐based aerosol delivered nose‐only in free‐breathing healthy rats, a T1‐weighted 3D UTE sequence was applied in a clinical 1.5 Tesla scanner. Administration lasted 14 min, and the experiment was performed on six rats. A contrast‐enhanced quantitative analysis was done.
Results
Fifty percent signal enhancement was obtained in the lung parenchyma. Lung clearance of the contrast agent was evaluated to be 14% per h (corresponding to a characteristic clearance time of 3.6 h) and aerosol deposition was shown to be homogeneous throughout the lung in healthy rats. The total deposited dose was estimated to be 1.05 µmol/kg body weight, and the concentration precision was 0.02 mM.
Conclusion
The UTE protocol with nebulized Gd‐DOTA is replicable to significantly enhance the lung parenchyma and to map aerosol deposition. This functional strategy, applied in a clinical system with a clinical nebulization setup and a low inhaled dose, suggests a feasible translation to human. Magn Reson Med 75:594–605, 2016. © 2015 Wiley Periodicals, Inc.</description><subject>Administration, Inhalation</subject><subject>Administration, Intranasal</subject><subject>aerosol deposition</subject><subject>Aerosol research</subject><subject>Aerosols</subject><subject>Animals</subject><subject>Body weight</subject><subject>Breathing</subject><subject>Contrast agents</subject><subject>contrast enhancement</subject><subject>Contrast media</subject><subject>Contrast Media - administration & dosage</subject><subject>Drug delivery</subject><subject>Feasibility Studies</subject><subject>Gadolinium</subject><subject>Gd-DOTA</subject><subject>Heterocyclic Compounds - administration & dosage</subject><subject>Image Processing, Computer-Assisted - methods</subject><subject>Life Sciences</subject><subject>Lung - anatomy & histology</subject><subject>lung MRI</subject><subject>Lungs</subject><subject>Magnetic resonance imaging</subject><subject>Magnetic Resonance Imaging, Cine - methods</subject><subject>Male</subject><subject>nebulization</subject><subject>Organometallic Compounds - administration & dosage</subject><subject>Parenchyma</subject><subject>Particle deposition</subject><subject>Physics</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Respiration</subject><subject>Toxicology</subject><subject>ultra-short echo</subject><issn>0740-3194</issn><issn>1522-2594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90UFv0zAUB_AIgVg3OPAFkCUu7JDOduI4PnYD2kHLpKkIbpbjvLQZTlxsB-iX4DPjkq0HJDjZsn7v6fn9k-QFwVOCMb3oXDelrCD8UTIhjNKUMpE_TiaY5zjNiMhPklPv7zDGQvD8aXJCWYlFnueT5NcMnPXWoBp21rehtT1qexS2gMzQbzyyDfI72wfVgx282aPKgQrbtt8gp4JHgz9c53X65mY9SyvloUY6eqd8QGoDfTSqr9Fg4lPqt9YFBHprUWg7QKvba6QCIlOG1uCNepY8aZTx8Pz-PEs-vXu7vlqky5v59dVsmWpWcp4WFa51VmrdgKZlVWKCG1zwWjVcM8VoQRQHrXFTAedC1LhmrCZZqRpSRMCzs-R87LtVRu5c2ym3l1a1cjFbysMbpjTuqiy-k2hfj3bn7LcBfJBd6zUYM65EEs6LgpWU40hf_UXv7OD6-BNJGRaUZoRk_1OEM85EcWDHEXUMyDtojnMSLA-xyxi7_BN7tC_vOw5VB_VRPuQcwcUIfrQG9v_uJFe3q4eW6VjR-gA_jxXKfZUFzziTnz_O5eXqC38vyEJ-yH4DD9rEsw</recordid><startdate>201602</startdate><enddate>201602</enddate><creator>Wang, Hongchen</creator><creator>Sebrié, Catherine</creator><creator>Ruaud, Jean-Pierre</creator><creator>Guillot, Geneviève</creator><creator>Bouazizi-Verdier, Khaoula</creator><creator>Willoquet, Georges</creator><creator>Maître, Xavier</creator><creator>Darrasse, Luc</creator><creator>de Rochefort, Ludovic</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><general>Wiley</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>P64</scope><scope>7QO</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-7466-6452</orcidid></search><sort><creationdate>201602</creationdate><title>Aerosol deposition in the lungs of spontaneously breathing rats using Gd-DOTA-based contrast agents and ultra-short echo time MRI at 1.5 Tesla</title><author>Wang, Hongchen ; Sebrié, Catherine ; Ruaud, Jean-Pierre ; Guillot, Geneviève ; Bouazizi-Verdier, Khaoula ; Willoquet, Georges ; Maître, Xavier ; Darrasse, Luc ; de Rochefort, Ludovic</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5877-6b0dc38ccfec28b8010f067daf7c5a5261a7ecc0fbe7799d0d55d138af16c5a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Administration, Inhalation</topic><topic>Administration, Intranasal</topic><topic>aerosol deposition</topic><topic>Aerosol research</topic><topic>Aerosols</topic><topic>Animals</topic><topic>Body weight</topic><topic>Breathing</topic><topic>Contrast agents</topic><topic>contrast enhancement</topic><topic>Contrast media</topic><topic>Contrast Media - administration & dosage</topic><topic>Drug delivery</topic><topic>Feasibility Studies</topic><topic>Gadolinium</topic><topic>Gd-DOTA</topic><topic>Heterocyclic Compounds - administration & dosage</topic><topic>Image Processing, Computer-Assisted - methods</topic><topic>Life Sciences</topic><topic>Lung - anatomy & histology</topic><topic>lung MRI</topic><topic>Lungs</topic><topic>Magnetic resonance imaging</topic><topic>Magnetic Resonance Imaging, Cine - methods</topic><topic>Male</topic><topic>nebulization</topic><topic>Organometallic Compounds - administration & dosage</topic><topic>Parenchyma</topic><topic>Particle deposition</topic><topic>Physics</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Respiration</topic><topic>Toxicology</topic><topic>ultra-short echo</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Hongchen</creatorcontrib><creatorcontrib>Sebrié, Catherine</creatorcontrib><creatorcontrib>Ruaud, Jean-Pierre</creatorcontrib><creatorcontrib>Guillot, Geneviève</creatorcontrib><creatorcontrib>Bouazizi-Verdier, Khaoula</creatorcontrib><creatorcontrib>Willoquet, Georges</creatorcontrib><creatorcontrib>Maître, Xavier</creatorcontrib><creatorcontrib>Darrasse, Luc</creatorcontrib><creatorcontrib>de Rochefort, Ludovic</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Magnetic resonance in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Hongchen</au><au>Sebrié, Catherine</au><au>Ruaud, Jean-Pierre</au><au>Guillot, Geneviève</au><au>Bouazizi-Verdier, Khaoula</au><au>Willoquet, Georges</au><au>Maître, Xavier</au><au>Darrasse, Luc</au><au>de Rochefort, Ludovic</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aerosol deposition in the lungs of spontaneously breathing rats using Gd-DOTA-based contrast agents and ultra-short echo time MRI at 1.5 Tesla</atitle><jtitle>Magnetic resonance in medicine</jtitle><addtitle>Magn. Reson. Med</addtitle><date>2016-02</date><risdate>2016</risdate><volume>75</volume><issue>2</issue><spage>594</spage><epage>605</epage><pages>594-605</pages><issn>0740-3194</issn><eissn>1522-2594</eissn><coden>MRMEEN</coden><abstract>Purpose
Aerosol toxicology and drug delivery through the lungs, which depend on various parameters, require methods to quantify particle deposition. Intrapulmonary‐administered MRI contrast agent combined with lung‐specific imaging sequences has been proposed as a high performance technique for aerosol research. Here, aerosol deposition is assessed using ultra‐short echo (UTE) sequences.
Methods
Before and after administration of Gd‐DOTA‐based aerosol delivered nose‐only in free‐breathing healthy rats, a T1‐weighted 3D UTE sequence was applied in a clinical 1.5 Tesla scanner. Administration lasted 14 min, and the experiment was performed on six rats. A contrast‐enhanced quantitative analysis was done.
Results
Fifty percent signal enhancement was obtained in the lung parenchyma. Lung clearance of the contrast agent was evaluated to be 14% per h (corresponding to a characteristic clearance time of 3.6 h) and aerosol deposition was shown to be homogeneous throughout the lung in healthy rats. The total deposited dose was estimated to be 1.05 µmol/kg body weight, and the concentration precision was 0.02 mM.
Conclusion
The UTE protocol with nebulized Gd‐DOTA is replicable to significantly enhance the lung parenchyma and to map aerosol deposition. This functional strategy, applied in a clinical system with a clinical nebulization setup and a low inhaled dose, suggests a feasible translation to human. Magn Reson Med 75:594–605, 2016. © 2015 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>25809444</pmid><doi>10.1002/mrm.25617</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-7466-6452</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Administration, Inhalation Administration, Intranasal aerosol deposition Aerosol research Aerosols Animals Body weight Breathing Contrast agents contrast enhancement Contrast media Contrast Media - administration & dosage Drug delivery Feasibility Studies Gadolinium Gd-DOTA Heterocyclic Compounds - administration & dosage Image Processing, Computer-Assisted - methods Life Sciences Lung - anatomy & histology lung MRI Lungs Magnetic resonance imaging Magnetic Resonance Imaging, Cine - methods Male nebulization Organometallic Compounds - administration & dosage Parenchyma Particle deposition Physics Rats Rats, Wistar Respiration Toxicology ultra-short echo |
| title | Aerosol deposition in the lungs of spontaneously breathing rats using Gd-DOTA-based contrast agents and ultra-short echo time MRI at 1.5 Tesla |
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