Protection of living yeast cells by micro-organized shells of natural polyelectrolytes
•Natural polyelectrolytes were used to encapsulate the yeast cells using LbL method.•Structure of thin layers was investigated by electron microscopy.•Electrostatic interactions of the system were characterized by infrared spectroscopy.•Encapsulated yeast cells preserved their viability and function...
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Veröffentlicht in: | Process biochemistry (1991) 2015-10, Vol.50 (10), p.1528-1536 |
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creator | Nguyen, Thanh Dat Guyot, Stéphane Lherminier, Jeannine Wache, Yves Saurel, Rémi Husson, Florence |
description | •Natural polyelectrolytes were used to encapsulate the yeast cells using LbL method.•Structure of thin layers was investigated by electron microscopy.•Electrostatic interactions of the system were characterized by infrared spectroscopy.•Encapsulated yeast cells preserved their viability and functionality.•Enhanced viability of yeast subjected to combined freezing and oxidative treatment.
Saccharomyces cerevisiae, a eukaryotic model organism, plays a key role in the oxidative stability of fermented products. In order to protect cells against environmental stresses, we report a method of modifying the cell surface architecture while maintaining the internal working properties of the system. The objective was to encapsulate living yeast cells in micro-organized polyelectrolyte shells using layer-by-layer (LbL) assembly. For the first time, the natural polyelectrolytes, β-lactoglobulin and sodium alginate, were alternately deposited on the surface of S. cerevisiae. Transmission electron microscopy coupled with immune-cytochemistry and scanning electron microscopy provided evidence of the polyelectrolyte layers around yeast cells. The electrostatic interactions between the yeast, β-lactoglobulin and alginate were assessed using Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR). We observed the preservation of yeast functionality and membrane integrity after encapsulation. Finally, we showed that the LbL method does not influence the yeast growth and improves the yeast viability after freezing treatments in combination or not with chemical (H2O2 or ethanol) stresses. |
doi_str_mv | 10.1016/j.procbio.2015.06.003 |
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Saccharomyces cerevisiae, a eukaryotic model organism, plays a key role in the oxidative stability of fermented products. In order to protect cells against environmental stresses, we report a method of modifying the cell surface architecture while maintaining the internal working properties of the system. The objective was to encapsulate living yeast cells in micro-organized polyelectrolyte shells using layer-by-layer (LbL) assembly. For the first time, the natural polyelectrolytes, β-lactoglobulin and sodium alginate, were alternately deposited on the surface of S. cerevisiae. Transmission electron microscopy coupled with immune-cytochemistry and scanning electron microscopy provided evidence of the polyelectrolyte layers around yeast cells. The electrostatic interactions between the yeast, β-lactoglobulin and alginate were assessed using Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR). We observed the preservation of yeast functionality and membrane integrity after encapsulation. Finally, we showed that the LbL method does not influence the yeast growth and improves the yeast viability after freezing treatments in combination or not with chemical (H2O2 or ethanol) stresses.</description><identifier>ISSN: 1359-5113</identifier><identifier>EISSN: 1873-3298</identifier><identifier>DOI: 10.1016/j.procbio.2015.06.003</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Architecture ; Chemical and Process Engineering ; Encapsulation ; Engineering Sciences ; Ethanol ; Ethyl alcohol ; Food engineering ; Freezing ; FTIR ; Immuno-electron microscopy ; Layer-by-layer ; Life Sciences ; Polyelectrolytes ; Saccharomyces cerevisiae ; Scanning electron microscopy ; Stresses ; Yeast ; β-Lactoglobulin</subject><ispartof>Process biochemistry (1991), 2015-10, Vol.50 (10), p.1528-1536</ispartof><rights>2015 Elsevier Ltd</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-4d02dd777f562ee27d85bbb7909e3ce8a0d6df2576e5b2da5d18cfa78825c7953</citedby><cites>FETCH-LOGICAL-c446t-4d02dd777f562ee27d85bbb7909e3ce8a0d6df2576e5b2da5d18cfa78825c7953</cites><orcidid>0000-0002-5337-3420 ; 0000-0002-5023-7247 ; 0000-0002-1832-8990 ; 0000-0002-2316-234X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S135951131530012X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://institut-agro-dijon.hal.science/hal-02290612$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Nguyen, Thanh Dat</creatorcontrib><creatorcontrib>Guyot, Stéphane</creatorcontrib><creatorcontrib>Lherminier, Jeannine</creatorcontrib><creatorcontrib>Wache, Yves</creatorcontrib><creatorcontrib>Saurel, Rémi</creatorcontrib><creatorcontrib>Husson, Florence</creatorcontrib><title>Protection of living yeast cells by micro-organized shells of natural polyelectrolytes</title><title>Process biochemistry (1991)</title><description>•Natural polyelectrolytes were used to encapsulate the yeast cells using LbL method.•Structure of thin layers was investigated by electron microscopy.•Electrostatic interactions of the system were characterized by infrared spectroscopy.•Encapsulated yeast cells preserved their viability and functionality.•Enhanced viability of yeast subjected to combined freezing and oxidative treatment.
Saccharomyces cerevisiae, a eukaryotic model organism, plays a key role in the oxidative stability of fermented products. In order to protect cells against environmental stresses, we report a method of modifying the cell surface architecture while maintaining the internal working properties of the system. The objective was to encapsulate living yeast cells in micro-organized polyelectrolyte shells using layer-by-layer (LbL) assembly. For the first time, the natural polyelectrolytes, β-lactoglobulin and sodium alginate, were alternately deposited on the surface of S. cerevisiae. Transmission electron microscopy coupled with immune-cytochemistry and scanning electron microscopy provided evidence of the polyelectrolyte layers around yeast cells. The electrostatic interactions between the yeast, β-lactoglobulin and alginate were assessed using Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR). We observed the preservation of yeast functionality and membrane integrity after encapsulation. Finally, we showed that the LbL method does not influence the yeast growth and improves the yeast viability after freezing treatments in combination or not with chemical (H2O2 or ethanol) stresses.</description><subject>Architecture</subject><subject>Chemical and Process Engineering</subject><subject>Encapsulation</subject><subject>Engineering Sciences</subject><subject>Ethanol</subject><subject>Ethyl alcohol</subject><subject>Food engineering</subject><subject>Freezing</subject><subject>FTIR</subject><subject>Immuno-electron microscopy</subject><subject>Layer-by-layer</subject><subject>Life Sciences</subject><subject>Polyelectrolytes</subject><subject>Saccharomyces cerevisiae</subject><subject>Scanning electron microscopy</subject><subject>Stresses</subject><subject>Yeast</subject><subject>β-Lactoglobulin</subject><issn>1359-5113</issn><issn>1873-3298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkU1LAzEQhhdR8PMnCHvUw66ZpEl2TyLFLyjoQb2GbDLbpmw3NdkW6q83tcWrnmaYPPMyed8suwRSAgFxMy-XwZvG-ZIS4CURJSHsIDuBSrKC0bo6TD3jdcEB2HF2GuM8AQBATrKP1-AHNIPzfe7bvHNr10_zDeo45Aa7LubNJl84E3zhw1T37gttHmc_L4nv9bAKusuXvttgl3RCagaM59lRq7uIF_t6lr0_3L-Nn4rJy-Pz-G5SmNFIDMXIEmqtlLLlgiJSaSveNI2sSY3MYKWJFbalXArkDbWaW6hMq2VVUW5kzdlZdr3TnelOLYNb6LBRXjv1dDdR2xmhtCYC6BoSe7Vjk1ufK4yDWri4_aPu0a-iAlkJEMBA_AMVlPERESShfIcmi2IM2P6eAURt41FztY9HbeNRRKjkftq73e1hsmftMKhoHPYGrQvJR2W9-0PhGzaXm9w</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Nguyen, Thanh Dat</creator><creator>Guyot, Stéphane</creator><creator>Lherminier, Jeannine</creator><creator>Wache, Yves</creator><creator>Saurel, Rémi</creator><creator>Husson, Florence</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7U5</scope><scope>F28</scope><scope>L7M</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-5337-3420</orcidid><orcidid>https://orcid.org/0000-0002-5023-7247</orcidid><orcidid>https://orcid.org/0000-0002-1832-8990</orcidid><orcidid>https://orcid.org/0000-0002-2316-234X</orcidid></search><sort><creationdate>20151001</creationdate><title>Protection of living yeast cells by micro-organized shells of natural polyelectrolytes</title><author>Nguyen, Thanh Dat ; 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Saccharomyces cerevisiae, a eukaryotic model organism, plays a key role in the oxidative stability of fermented products. In order to protect cells against environmental stresses, we report a method of modifying the cell surface architecture while maintaining the internal working properties of the system. The objective was to encapsulate living yeast cells in micro-organized polyelectrolyte shells using layer-by-layer (LbL) assembly. For the first time, the natural polyelectrolytes, β-lactoglobulin and sodium alginate, were alternately deposited on the surface of S. cerevisiae. Transmission electron microscopy coupled with immune-cytochemistry and scanning electron microscopy provided evidence of the polyelectrolyte layers around yeast cells. The electrostatic interactions between the yeast, β-lactoglobulin and alginate were assessed using Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR). We observed the preservation of yeast functionality and membrane integrity after encapsulation. Finally, we showed that the LbL method does not influence the yeast growth and improves the yeast viability after freezing treatments in combination or not with chemical (H2O2 or ethanol) stresses.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.procbio.2015.06.003</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5337-3420</orcidid><orcidid>https://orcid.org/0000-0002-5023-7247</orcidid><orcidid>https://orcid.org/0000-0002-1832-8990</orcidid><orcidid>https://orcid.org/0000-0002-2316-234X</orcidid></addata></record> |
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subjects | Architecture Chemical and Process Engineering Encapsulation Engineering Sciences Ethanol Ethyl alcohol Food engineering Freezing FTIR Immuno-electron microscopy Layer-by-layer Life Sciences Polyelectrolytes Saccharomyces cerevisiae Scanning electron microscopy Stresses Yeast β-Lactoglobulin |
title | Protection of living yeast cells by micro-organized shells of natural polyelectrolytes |
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