Improvement of nonviral p53 gene transfer in human carcinoma cells using glucosylated polyethylenimine derivatives

Polyethylenimine (PEI) derivatives are potent polycationic nonviral vectors for gene transfer. The gene transfer efficiency of glucosylated and galactosylated PEI derivatives was optimized using green fluorescent protein gene as reporter gene in FaDu and PANC3 human carcinoma cell lines. Glucosylate...

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Veröffentlicht in:	Cancer gene therapy 2001-03, Vol.8 (3), p.203-210
Hauptverfasser:	Merlin, J L, Dolivet, G, Dubessy, C, Festor, E, Parache, R M, Verneuil, L, Erbacher, P, Behr, J P, Guillemin, F
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis Apoptosis - genetics Blotting, Western Breast Neoplasms - genetics Breast Neoplasms - pathology Cancer Carcinoma - genetics Carcinoma - pathology Cell number Cytotoxicity deletion Deoxyribonucleic acid DNA Efficiency Endocytosis - physiology Expression vectors Female Formazans - analysis Gene deletion Gene Expression Gene therapy Gene Transfer Techniques Genes, p53 - physiology Genetic Vectors Glycosylation Green fluorescent protein Green Fluorescent Proteins Humans Indicators and Reagents Life Sciences Luciferases - analysis Luciferases - genetics Luminescent Proteins - analysis Luminescent Proteins - genetics Microscopy, Fluorescence p53 Protein Pancreatic Neoplasms - genetics Pancreatic Neoplasms - pathology Pharyngeal Neoplasms - genetics Pharyngeal Neoplasms - pathology polyethyleneimine Polyethyleneimine - analogs & derivatives Proteins Reporter gene Reverse Transcriptase Polymerase Chain Reaction Tetrazolium Salts - metabolism Transfection Tumor cell lines Tumor Cells, Cultured
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