Protein complexes associated with β‐catenin differentially influence the differentiation profile of neonatal and adult CD8+ T cells
The canonical Wnt signaling pathway is a master cell regulator involved in CD8+ T cell proliferation and differentiation. In human CD8+ T cells, this pathway induces differentiation into memory cells or a “stem cell memory like” population, which is preferentially present in cord blood. To better un...
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| Veröffentlicht in: | Journal of cellular physiology 2019-10, Vol.234 (10), p.18639-18652 |
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| creator | Hernández‐Acevedo, Gerson N. López‐Portales, Oscar H. Gutiérrez‐Reyna, Darely Y. Cuevas‐Fernández, Erick Kempis‐Calanis, Linda A. Labastida‐Conde, Rosario G. Aguilar‐Luviano, Oscar B. Ramírez‐Pliego, Oscar Spicuglia, Salvatore Lino‐Alfaro, Bárbara Chagolla‐López, Alicia González‐de la Vara, Luis E. Santana, María Angélica |
| description | The canonical Wnt signaling pathway is a master cell regulator involved in CD8+ T cell proliferation and differentiation. In human CD8+ T cells, this pathway induces differentiation into memory cells or a “stem cell memory like” population, which is preferentially present in cord blood. To better understand the role of canonical Wnt signals in neonatal or adult blood, we compared the proteins associated with β‐catenin, in nonstimulated and Wnt3a‐stimulated human neonatal and adult naive CD8+ T cells. Differentially recruited proteins established different complexes in adult and neonatal cells. In the former, β‐catenin‐associated proteins were linked to cell signaling and immunological functions, whereas those of neonates were linked to proliferation and metabolism. Wnt3a stimulation led to the recruitment and overexpression of Wnt11 in adult cells and Wnt5a in neonatal cells, suggesting a differential connexion with planar polarity and Wnt/Ca2+ noncanonical pathways, respectively. The chromatin immunoprecipitation polymerase chain reaction β‐catenin was recruited to a higher level on the promoters of cell renewal genes in neonatal cells and of differentiation genes in those of adults. We found a preferential association of β‐catenin with CBP in neonatal cells and with p300 in the adult samples, which could be involved in a higher self‐renewal capacity of the neonatal cells and memory commitment in those of adults. Altogether, our results show that different proteins associated with β‐catenin during Wnt3a activation mediate a differential response of neonatal and adult human CD8+ T cells.
Specific proteins were found associated with β‐catenin in nonstimulated and Wnt3a‐stimulated human neonatal cells as compared with those of adults. Differentially recruited proteins established different complexes in adult and neonatal cells. These differential proteins could be responsible for a differential response of neonatal and adult human CD8+T cells. |
| doi_str_mv | 10.1002/jcp.28502 |
| format | Article |
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Specific proteins were found associated with β‐catenin in nonstimulated and Wnt3a‐stimulated human neonatal cells as compared with those of adults. Differentially recruited proteins established different complexes in adult and neonatal cells. These differential proteins could be responsible for a differential response of neonatal and adult human CD8+T cells.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.28502</identifier><identifier>PMID: 30924167</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adult ; Adults ; beta Catenin - metabolism ; Blood ; Calcium ; Calcium ions ; Catenin ; CBP/p300 ; CD8 antigen ; CD8+ T cells ; CD8-Positive T-Lymphocytes - cytology ; CD8-Positive T-Lymphocytes - metabolism ; Cell activation ; Cell Differentiation ; Cell proliferation ; Cell self-renewal ; Chromatin ; Computer memory ; Cord blood ; Differentiation ; Event-related potentials ; Gene Expression Regulation ; Genes ; Humans ; Immunology ; Immunoprecipitation ; Infant, Newborn ; Life Sciences ; Lymphocytes ; Lymphocytes T ; Memory cells ; Metabolism ; Multiprotein Complexes - metabolism ; neonate immunity ; Neonates ; Polarity ; Polymerase chain reaction ; Promoter Regions, Genetic - genetics ; Protein Binding ; Protein Interaction Mapping ; Proteins ; proteomics ; Signal transduction ; Signaling ; Stem cells ; Wnt protein ; Wnt Signaling Pathway ; β‐catenin</subject><ispartof>Journal of cellular physiology, 2019-10, Vol.234 (10), p.18639-18652</ispartof><rights>2019 Wiley Periodicals, Inc.</rights><rights>Attribution</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4222-6c725d465dc79ee5fca217a01268b320870da91c1287fde0832614e44c270dbb3</citedby><cites>FETCH-LOGICAL-c4222-6c725d465dc79ee5fca217a01268b320870da91c1287fde0832614e44c270dbb3</cites><orcidid>0000-0001-8373-7873 ; 0000-0002-6998-0647 ; 0000-0002-8101-7108</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.28502$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.28502$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30924167$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://amu.hal.science/hal-02154920$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Hernández‐Acevedo, Gerson N.</creatorcontrib><creatorcontrib>López‐Portales, Oscar H.</creatorcontrib><creatorcontrib>Gutiérrez‐Reyna, Darely Y.</creatorcontrib><creatorcontrib>Cuevas‐Fernández, Erick</creatorcontrib><creatorcontrib>Kempis‐Calanis, Linda A.</creatorcontrib><creatorcontrib>Labastida‐Conde, Rosario G.</creatorcontrib><creatorcontrib>Aguilar‐Luviano, Oscar B.</creatorcontrib><creatorcontrib>Ramírez‐Pliego, Oscar</creatorcontrib><creatorcontrib>Spicuglia, Salvatore</creatorcontrib><creatorcontrib>Lino‐Alfaro, Bárbara</creatorcontrib><creatorcontrib>Chagolla‐López, Alicia</creatorcontrib><creatorcontrib>González‐de la Vara, Luis E.</creatorcontrib><creatorcontrib>Santana, María Angélica</creatorcontrib><title>Protein complexes associated with β‐catenin differentially influence the differentiation profile of neonatal and adult CD8+ T cells</title><title>Journal of cellular physiology</title><addtitle>J Cell Physiol</addtitle><description>The canonical Wnt signaling pathway is a master cell regulator involved in CD8+ T cell proliferation and differentiation. In human CD8+ T cells, this pathway induces differentiation into memory cells or a “stem cell memory like” population, which is preferentially present in cord blood. To better understand the role of canonical Wnt signals in neonatal or adult blood, we compared the proteins associated with β‐catenin, in nonstimulated and Wnt3a‐stimulated human neonatal and adult naive CD8+ T cells. Differentially recruited proteins established different complexes in adult and neonatal cells. In the former, β‐catenin‐associated proteins were linked to cell signaling and immunological functions, whereas those of neonates were linked to proliferation and metabolism. Wnt3a stimulation led to the recruitment and overexpression of Wnt11 in adult cells and Wnt5a in neonatal cells, suggesting a differential connexion with planar polarity and Wnt/Ca2+ noncanonical pathways, respectively. The chromatin immunoprecipitation polymerase chain reaction β‐catenin was recruited to a higher level on the promoters of cell renewal genes in neonatal cells and of differentiation genes in those of adults. We found a preferential association of β‐catenin with CBP in neonatal cells and with p300 in the adult samples, which could be involved in a higher self‐renewal capacity of the neonatal cells and memory commitment in those of adults. Altogether, our results show that different proteins associated with β‐catenin during Wnt3a activation mediate a differential response of neonatal and adult human CD8+ T cells.
Specific proteins were found associated with β‐catenin in nonstimulated and Wnt3a‐stimulated human neonatal cells as compared with those of adults. Differentially recruited proteins established different complexes in adult and neonatal cells. These differential proteins could be responsible for a differential response of neonatal and adult human CD8+T cells.</description><subject>Adult</subject><subject>Adults</subject><subject>beta Catenin - metabolism</subject><subject>Blood</subject><subject>Calcium</subject><subject>Calcium ions</subject><subject>Catenin</subject><subject>CBP/p300</subject><subject>CD8 antigen</subject><subject>CD8+ T cells</subject><subject>CD8-Positive T-Lymphocytes - cytology</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>Cell activation</subject><subject>Cell Differentiation</subject><subject>Cell proliferation</subject><subject>Cell self-renewal</subject><subject>Chromatin</subject><subject>Computer memory</subject><subject>Cord blood</subject><subject>Differentiation</subject><subject>Event-related potentials</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Humans</subject><subject>Immunology</subject><subject>Immunoprecipitation</subject><subject>Infant, Newborn</subject><subject>Life Sciences</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Memory cells</subject><subject>Metabolism</subject><subject>Multiprotein Complexes - metabolism</subject><subject>neonate immunity</subject><subject>Neonates</subject><subject>Polarity</subject><subject>Polymerase chain reaction</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein Binding</subject><subject>Protein Interaction Mapping</subject><subject>Proteins</subject><subject>proteomics</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>Stem cells</subject><subject>Wnt protein</subject><subject>Wnt Signaling Pathway</subject><subject>β‐catenin</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1uEzEUhS0EoqGw4AWQJTYgNO31Hc-Pl1X4KSgSXZS15XjuKI4cO4xnWrJjxZpn4UF4CJ4Eh5SCkFhZ9vl0fM89jD0WcCIA8HRttyfYVoB32EyAagpZV3iXzbImClVJccQepLQGAKXK8j47KkGhFHUzY18uhjiSC9zGzdbTJ0rcpBStMyN1_NqNK_7924_PX22-h4x1ru9poDA64_2Ou9D7iYIlPq7ob3F0MfDtEHvniceeB4rBjMZzEzpuusmPfP6yfcEvuSXv00N2rzc-0aOb85h9eP3qcn5eLN6_eTs_WxRWImJR2warLqfrbKOIqt4aFI0BgXW7LBHaBjqjhBXYNn1H0JZYC0lSWszKclkes-cH35Xxeju4jRl2Ohqnz88Wev-WN1ZJhXAlMvvswOYYHydKo964tJ_W5DBT0ogATVMKwIw-_Qddx2kIOUmmpJJQVkr8-dwOMaWB-tsJBOh9kToXqX8VmdknN47TckPdLfm7uQycHoDrvOHd_530u_nFwfInrAqoWw</recordid><startdate>201910</startdate><enddate>201910</enddate><creator>Hernández‐Acevedo, Gerson N.</creator><creator>López‐Portales, Oscar H.</creator><creator>Gutiérrez‐Reyna, Darely Y.</creator><creator>Cuevas‐Fernández, Erick</creator><creator>Kempis‐Calanis, Linda A.</creator><creator>Labastida‐Conde, Rosario G.</creator><creator>Aguilar‐Luviano, Oscar B.</creator><creator>Ramírez‐Pliego, Oscar</creator><creator>Spicuglia, Salvatore</creator><creator>Lino‐Alfaro, Bárbara</creator><creator>Chagolla‐López, Alicia</creator><creator>González‐de la Vara, Luis E.</creator><creator>Santana, María Angélica</creator><general>Wiley Subscription Services, Inc</general><general>Wiley</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0001-8373-7873</orcidid><orcidid>https://orcid.org/0000-0002-6998-0647</orcidid><orcidid>https://orcid.org/0000-0002-8101-7108</orcidid></search><sort><creationdate>201910</creationdate><title>Protein complexes associated with β‐catenin differentially influence the differentiation profile of neonatal and adult CD8+ T cells</title><author>Hernández‐Acevedo, Gerson N. ; López‐Portales, Oscar H. ; Gutiérrez‐Reyna, Darely Y. ; Cuevas‐Fernández, Erick ; Kempis‐Calanis, Linda A. ; Labastida‐Conde, Rosario G. ; Aguilar‐Luviano, Oscar B. ; Ramírez‐Pliego, Oscar ; Spicuglia, Salvatore ; Lino‐Alfaro, Bárbara ; Chagolla‐López, Alicia ; González‐de la Vara, Luis E. ; Santana, María Angélica</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4222-6c725d465dc79ee5fca217a01268b320870da91c1287fde0832614e44c270dbb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Adults</topic><topic>beta Catenin - metabolism</topic><topic>Blood</topic><topic>Calcium</topic><topic>Calcium ions</topic><topic>Catenin</topic><topic>CBP/p300</topic><topic>CD8 antigen</topic><topic>CD8+ T cells</topic><topic>CD8-Positive T-Lymphocytes - cytology</topic><topic>CD8-Positive T-Lymphocytes - metabolism</topic><topic>Cell activation</topic><topic>Cell Differentiation</topic><topic>Cell proliferation</topic><topic>Cell self-renewal</topic><topic>Chromatin</topic><topic>Computer memory</topic><topic>Cord blood</topic><topic>Differentiation</topic><topic>Event-related potentials</topic><topic>Gene Expression Regulation</topic><topic>Genes</topic><topic>Humans</topic><topic>Immunology</topic><topic>Immunoprecipitation</topic><topic>Infant, Newborn</topic><topic>Life Sciences</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Memory cells</topic><topic>Metabolism</topic><topic>Multiprotein Complexes - metabolism</topic><topic>neonate immunity</topic><topic>Neonates</topic><topic>Polarity</topic><topic>Polymerase chain reaction</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein Binding</topic><topic>Protein Interaction Mapping</topic><topic>Proteins</topic><topic>proteomics</topic><topic>Signal transduction</topic><topic>Signaling</topic><topic>Stem cells</topic><topic>Wnt protein</topic><topic>Wnt Signaling Pathway</topic><topic>β‐catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hernández‐Acevedo, Gerson N.</creatorcontrib><creatorcontrib>López‐Portales, Oscar H.</creatorcontrib><creatorcontrib>Gutiérrez‐Reyna, Darely Y.</creatorcontrib><creatorcontrib>Cuevas‐Fernández, Erick</creatorcontrib><creatorcontrib>Kempis‐Calanis, Linda A.</creatorcontrib><creatorcontrib>Labastida‐Conde, Rosario G.</creatorcontrib><creatorcontrib>Aguilar‐Luviano, Oscar B.</creatorcontrib><creatorcontrib>Ramírez‐Pliego, Oscar</creatorcontrib><creatorcontrib>Spicuglia, Salvatore</creatorcontrib><creatorcontrib>Lino‐Alfaro, Bárbara</creatorcontrib><creatorcontrib>Chagolla‐López, Alicia</creatorcontrib><creatorcontrib>González‐de la Vara, Luis E.</creatorcontrib><creatorcontrib>Santana, María Angélica</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hernández‐Acevedo, Gerson N.</au><au>López‐Portales, Oscar H.</au><au>Gutiérrez‐Reyna, Darely Y.</au><au>Cuevas‐Fernández, Erick</au><au>Kempis‐Calanis, Linda A.</au><au>Labastida‐Conde, Rosario G.</au><au>Aguilar‐Luviano, Oscar B.</au><au>Ramírez‐Pliego, Oscar</au><au>Spicuglia, Salvatore</au><au>Lino‐Alfaro, Bárbara</au><au>Chagolla‐López, Alicia</au><au>González‐de la Vara, Luis E.</au><au>Santana, María Angélica</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein complexes associated with β‐catenin differentially influence the differentiation profile of neonatal and adult CD8+ T cells</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J Cell Physiol</addtitle><date>2019-10</date><risdate>2019</risdate><volume>234</volume><issue>10</issue><spage>18639</spage><epage>18652</epage><pages>18639-18652</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>The canonical Wnt signaling pathway is a master cell regulator involved in CD8+ T cell proliferation and differentiation. In human CD8+ T cells, this pathway induces differentiation into memory cells or a “stem cell memory like” population, which is preferentially present in cord blood. To better understand the role of canonical Wnt signals in neonatal or adult blood, we compared the proteins associated with β‐catenin, in nonstimulated and Wnt3a‐stimulated human neonatal and adult naive CD8+ T cells. Differentially recruited proteins established different complexes in adult and neonatal cells. In the former, β‐catenin‐associated proteins were linked to cell signaling and immunological functions, whereas those of neonates were linked to proliferation and metabolism. Wnt3a stimulation led to the recruitment and overexpression of Wnt11 in adult cells and Wnt5a in neonatal cells, suggesting a differential connexion with planar polarity and Wnt/Ca2+ noncanonical pathways, respectively. The chromatin immunoprecipitation polymerase chain reaction β‐catenin was recruited to a higher level on the promoters of cell renewal genes in neonatal cells and of differentiation genes in those of adults. We found a preferential association of β‐catenin with CBP in neonatal cells and with p300 in the adult samples, which could be involved in a higher self‐renewal capacity of the neonatal cells and memory commitment in those of adults. Altogether, our results show that different proteins associated with β‐catenin during Wnt3a activation mediate a differential response of neonatal and adult human CD8+ T cells.
Specific proteins were found associated with β‐catenin in nonstimulated and Wnt3a‐stimulated human neonatal cells as compared with those of adults. Differentially recruited proteins established different complexes in adult and neonatal cells. These differential proteins could be responsible for a differential response of neonatal and adult human CD8+T cells.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30924167</pmid><doi>10.1002/jcp.28502</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-8373-7873</orcidid><orcidid>https://orcid.org/0000-0002-6998-0647</orcidid><orcidid>https://orcid.org/0000-0002-8101-7108</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Adults beta Catenin - metabolism Blood Calcium Calcium ions Catenin CBP/p300 CD8 antigen CD8+ T cells CD8-Positive T-Lymphocytes - cytology CD8-Positive T-Lymphocytes - metabolism Cell activation Cell Differentiation Cell proliferation Cell self-renewal Chromatin Computer memory Cord blood Differentiation Event-related potentials Gene Expression Regulation Genes Humans Immunology Immunoprecipitation Infant, Newborn Life Sciences Lymphocytes Lymphocytes T Memory cells Metabolism Multiprotein Complexes - metabolism neonate immunity Neonates Polarity Polymerase chain reaction Promoter Regions, Genetic - genetics Protein Binding Protein Interaction Mapping Proteins proteomics Signal transduction Signaling Stem cells Wnt protein Wnt Signaling Pathway β‐catenin |
| title | Protein complexes associated with β‐catenin differentially influence the differentiation profile of neonatal and adult CD8+ T cells |
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