Imaging of dense cell cultures by multiwavelength lens‐free video microscopy
They present results for lens‐free microscopy for the imaging of dense cell culture. With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction algorithm. This allows for a fast and efficie...
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| Veröffentlicht in: | Cytometry. Part A 2017-05, Vol.91 (5), p.433-442 |
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| container_title | Cytometry. Part A |
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| creator | Allier, C. Morel, S. Vincent, R. Ghenim, L. Navarro, F. Menneteau, M. Bordy, T. Hervé, L. Cioni, O. Gidrol, X. Usson, Y. Dinten, J.‐M. |
| description | They present results for lens‐free microscopy for the imaging of dense cell culture. With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction algorithm. This allows for a fast and efficient reconstruction of the phase image of densely packed cells (up to 700 cells/mm2) over a large field of view of 29.4 mm2. Combined with the compactness of the system which fits altogether inside an incubator, lens‐free microscopy becomes a unique tool to monitor cell cultures over several days. The high contrast phase shift images provide robust cell segmentation and tracking, and enable high throughput monitoring of individual cell dimensions, dry mass, and motility. They tested the multiwavelength lens‐free video‐microscope over a broad range of cell lines, including mesenchymal, endothelial, and epithelial cells. © 2017 International Society for Advancement of Cytometry |
| doi_str_mv | 10.1002/cyto.a.23079 |
| format | Article |
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With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction algorithm. This allows for a fast and efficient reconstruction of the phase image of densely packed cells (up to 700 cells/mm2) over a large field of view of 29.4 mm2. Combined with the compactness of the system which fits altogether inside an incubator, lens‐free microscopy becomes a unique tool to monitor cell cultures over several days. The high contrast phase shift images provide robust cell segmentation and tracking, and enable high throughput monitoring of individual cell dimensions, dry mass, and motility. They tested the multiwavelength lens‐free video‐microscope over a broad range of cell lines, including mesenchymal, endothelial, and epithelial cells. © 2017 International Society for Advancement of Cytometry</description><identifier>ISSN: 1552-4922</identifier><identifier>EISSN: 1552-4930</identifier><identifier>DOI: 10.1002/cyto.a.23079</identifier><identifier>PMID: 28240818</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Biotechnology ; Cell Count - methods ; Cell culture ; Cell Culture Techniques ; Cell lines ; Cell Movement - genetics ; Cytometry ; dense cell cultures ; Epithelial cells ; Epithelial Cells - cytology ; Field of view ; Holography - methods ; Humans ; Illumination ; Image contrast ; Image processing ; Image reconstruction ; Image segmentation ; Lenses ; lens‐free microscopy ; Life Sciences ; Mesenchyme ; Microscopy ; Microscopy, Video - methods ; Phase shift ; quantitative microscopy ; video cytometry ; Wavelengths</subject><ispartof>Cytometry. 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Part A</title><addtitle>Cytometry A</addtitle><description>They present results for lens‐free microscopy for the imaging of dense cell culture. With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction algorithm. This allows for a fast and efficient reconstruction of the phase image of densely packed cells (up to 700 cells/mm2) over a large field of view of 29.4 mm2. Combined with the compactness of the system which fits altogether inside an incubator, lens‐free microscopy becomes a unique tool to monitor cell cultures over several days. The high contrast phase shift images provide robust cell segmentation and tracking, and enable high throughput monitoring of individual cell dimensions, dry mass, and motility. They tested the multiwavelength lens‐free video‐microscope over a broad range of cell lines, including mesenchymal, endothelial, and epithelial cells. © 2017 International Society for Advancement of Cytometry</description><subject>Biotechnology</subject><subject>Cell Count - methods</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell lines</subject><subject>Cell Movement - genetics</subject><subject>Cytometry</subject><subject>dense cell cultures</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - cytology</subject><subject>Field of view</subject><subject>Holography - methods</subject><subject>Humans</subject><subject>Illumination</subject><subject>Image contrast</subject><subject>Image processing</subject><subject>Image reconstruction</subject><subject>Image segmentation</subject><subject>Lenses</subject><subject>lens‐free microscopy</subject><subject>Life Sciences</subject><subject>Mesenchyme</subject><subject>Microscopy</subject><subject>Microscopy, Video - methods</subject><subject>Phase shift</subject><subject>quantitative microscopy</subject><subject>video cytometry</subject><subject>Wavelengths</subject><issn>1552-4922</issn><issn>1552-4930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90btOwzAUBmALgbhvzMgSC0i0HF-S2GNVcZMqWGBgspzkpAQlcYmbomw8As_Ik5AQ6MDAdI6sT7989BNyxGDMAPhF0i7d2I65gEhvkF0WBHwktYDN9c75Dtnz_gVABCD4NtnhiktQTO2Su9vSzvNqTl1GU6w80gSLgiZNsWxq9DRuadnt-ZtdYYHVfPlMu-E_3z-yGpGu8hQdLfOkdj5xi_aAbGW28Hj4M_fJ49Xlw_RmNLu_vp1OZqNEilCPUEEQijhlNsVMcGQxxHGMlmeWhQJDRC01ZJIFivNIhRYBWaogUaAjaa3YJ2dD7rMtzKLOS1u3xtnc3Exmpn8DDjoQWq1YZ08Hu6jda4N-acrc91faCl3jDVMRDxQwITp68oe-uKauuksM0xxkGGkuO3U-qP5qX2O2_gED03di-k6MNd-ddPz4J7SJS0zX-LeEDsgBvOUFtv-GmenTw_1kyP0CIJmYoA</recordid><startdate>201705</startdate><enddate>201705</enddate><creator>Allier, C.</creator><creator>Morel, S.</creator><creator>Vincent, R.</creator><creator>Ghenim, L.</creator><creator>Navarro, F.</creator><creator>Menneteau, M.</creator><creator>Bordy, T.</creator><creator>Hervé, L.</creator><creator>Cioni, O.</creator><creator>Gidrol, X.</creator><creator>Usson, Y.</creator><creator>Dinten, J.‐M.</creator><general>Wiley Subscription Services, Inc</general><general>Wiley</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-0242-191X</orcidid><orcidid>https://orcid.org/0000-0003-1482-3115</orcidid></search><sort><creationdate>201705</creationdate><title>Imaging of dense cell cultures by multiwavelength lens‐free video microscopy</title><author>Allier, C. ; Morel, S. ; Vincent, R. ; Ghenim, L. ; Navarro, F. ; Menneteau, M. ; Bordy, T. ; Hervé, L. ; Cioni, O. ; Gidrol, X. ; Usson, Y. ; Dinten, J.‐M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4369-e80563bd1adef32e1b0bbbea2fa163e6ee9490f415822786ae0e1d80c80974aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Biotechnology</topic><topic>Cell Count - methods</topic><topic>Cell culture</topic><topic>Cell Culture Techniques</topic><topic>Cell lines</topic><topic>Cell Movement - genetics</topic><topic>Cytometry</topic><topic>dense cell cultures</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - cytology</topic><topic>Field of view</topic><topic>Holography - methods</topic><topic>Humans</topic><topic>Illumination</topic><topic>Image contrast</topic><topic>Image processing</topic><topic>Image reconstruction</topic><topic>Image segmentation</topic><topic>Lenses</topic><topic>lens‐free microscopy</topic><topic>Life Sciences</topic><topic>Mesenchyme</topic><topic>Microscopy</topic><topic>Microscopy, Video - methods</topic><topic>Phase shift</topic><topic>quantitative microscopy</topic><topic>video cytometry</topic><topic>Wavelengths</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Allier, C.</creatorcontrib><creatorcontrib>Morel, S.</creatorcontrib><creatorcontrib>Vincent, R.</creatorcontrib><creatorcontrib>Ghenim, L.</creatorcontrib><creatorcontrib>Navarro, F.</creatorcontrib><creatorcontrib>Menneteau, M.</creatorcontrib><creatorcontrib>Bordy, T.</creatorcontrib><creatorcontrib>Hervé, L.</creatorcontrib><creatorcontrib>Cioni, O.</creatorcontrib><creatorcontrib>Gidrol, X.</creatorcontrib><creatorcontrib>Usson, Y.</creatorcontrib><creatorcontrib>Dinten, J.‐M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Cytometry. 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With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction algorithm. This allows for a fast and efficient reconstruction of the phase image of densely packed cells (up to 700 cells/mm2) over a large field of view of 29.4 mm2. Combined with the compactness of the system which fits altogether inside an incubator, lens‐free microscopy becomes a unique tool to monitor cell cultures over several days. The high contrast phase shift images provide robust cell segmentation and tracking, and enable high throughput monitoring of individual cell dimensions, dry mass, and motility. They tested the multiwavelength lens‐free video‐microscope over a broad range of cell lines, including mesenchymal, endothelial, and epithelial cells. © 2017 International Society for Advancement of Cytometry</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28240818</pmid><doi>10.1002/cyto.a.23079</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-0242-191X</orcidid><orcidid>https://orcid.org/0000-0003-1482-3115</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Cytometry. Part A, 2017-05, Vol.91 (5), p.433-442 |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Biotechnology Cell Count - methods Cell culture Cell Culture Techniques Cell lines Cell Movement - genetics Cytometry dense cell cultures Epithelial cells Epithelial Cells - cytology Field of view Holography - methods Humans Illumination Image contrast Image processing Image reconstruction Image segmentation Lenses lens‐free microscopy Life Sciences Mesenchyme Microscopy Microscopy, Video - methods Phase shift quantitative microscopy video cytometry Wavelengths |
| title | Imaging of dense cell cultures by multiwavelength lens‐free video microscopy |
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