drd2-cre:ribotag mouse line unravels the possible diversity of dopamine d2 receptor-expressing cells of the dorsal mouse hippocampus
ABSTRACT Increasing evidences suggest that dopamine facilitates the encoding of novel memories by the hippocampus. However, the role of dopamine D2 receptors (D2R) in such regulations remains elusive due to the lack of the precise identification of hippocampal D2R‐expressing cells. To address this i...
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| Veröffentlicht in: | Hippocampus 2015-07, Vol.25 (7), p.858-875 |
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| creator | Puighermanal, Emma Biever, Anne Espallergues, Julie Gangarossa, Giuseppe De Bundel, Dimitri Valjent, Emmanuel |
| description | ABSTRACT
Increasing evidences suggest that dopamine facilitates the encoding of novel memories by the hippocampus. However, the role of dopamine D2 receptors (D2R) in such regulations remains elusive due to the lack of the precise identification of hippocampal D2R‐expressing cells. To address this issue, mice expressing the ribosomal protein Rpl22 tagged with the hemagglutinin (HA) epitope were crossed with Drd2‐Cre mice allowing the selective expression of HA in D2R‐containing cells (Drd2‐Cre:RiboTag mice). This new transgenic model revealed a more widespread pattern of D2R‐expressing cells identified by HA immunoreactivity than the one initially reported in Drd2‐EGFP mice, in which the hilar mossy cells were the main neuronal population detectable. In Drd2‐Cre:RiboTag mice, scattered HA/GAD67‐positive neurons were detected throughout the CA1/CA3 subfields, being preferentially localized in stratum oriens and stratum lacunosum‐moleculare. At the cellular level, HA‐labeled cells located in CA1/CA3 subfields co‐localized with calcium‐binding proteins (parvalbumin, calbindin, and calretinin), neuropeptides (neuropeptide Y, somatostatin), and other markers (neuronal nitric oxide synthase, mGluR1α, reelin, coupTFII, and potassium channel‐interacting protein 1). These results suggest that in addition to the glutamatergic hilar mossy cells, D2R‐expressing cells constitute a subpopulation of GABAergic hippocampal interneurons. © 2014 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/hipo.22408 |
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Increasing evidences suggest that dopamine facilitates the encoding of novel memories by the hippocampus. However, the role of dopamine D2 receptors (D2R) in such regulations remains elusive due to the lack of the precise identification of hippocampal D2R‐expressing cells. To address this issue, mice expressing the ribosomal protein Rpl22 tagged with the hemagglutinin (HA) epitope were crossed with Drd2‐Cre mice allowing the selective expression of HA in D2R‐containing cells (Drd2‐Cre:RiboTag mice). This new transgenic model revealed a more widespread pattern of D2R‐expressing cells identified by HA immunoreactivity than the one initially reported in Drd2‐EGFP mice, in which the hilar mossy cells were the main neuronal population detectable. In Drd2‐Cre:RiboTag mice, scattered HA/GAD67‐positive neurons were detected throughout the CA1/CA3 subfields, being preferentially localized in stratum oriens and stratum lacunosum‐moleculare. At the cellular level, HA‐labeled cells located in CA1/CA3 subfields co‐localized with calcium‐binding proteins (parvalbumin, calbindin, and calretinin), neuropeptides (neuropeptide Y, somatostatin), and other markers (neuronal nitric oxide synthase, mGluR1α, reelin, coupTFII, and potassium channel‐interacting protein 1). These results suggest that in addition to the glutamatergic hilar mossy cells, D2R‐expressing cells constitute a subpopulation of GABAergic hippocampal interneurons. © 2014 Wiley Periodicals, Inc.</description><identifier>ISSN: 1050-9631</identifier><identifier>EISSN: 1098-1063</identifier><identifier>DOI: 10.1002/hipo.22408</identifier><identifier>PMID: 25545461</identifier><identifier>CODEN: HIPPEL</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Animals ; Calbindin 2 - metabolism ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism ; Channelrhodopsins ; dopamine D2 receptor ; Gene Expression Regulation - genetics ; Glutamate Decarboxylase - metabolism ; Green Fluorescent Proteins - genetics ; Green Fluorescent Proteins - metabolism ; hilar mossy cells ; Hippocampus - cytology ; interneurons ; KChIP1 ; Life Sciences ; Male ; Membrane Proteins - metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Microscopy, Confocal ; Nerve Tissue Proteins - metabolism ; Neurons - metabolism ; Receptors, Dopamine D2 - genetics ; Receptors, Dopamine D2 - metabolism ; Ribosomal Proteins - genetics ; Ribosomal Proteins - metabolism ; RiboTag mice ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism</subject><ispartof>Hippocampus, 2015-07, Vol.25 (7), p.858-875</ispartof><rights>2014 Wiley Periodicals, Inc.</rights><rights>2015 Wiley Periodicals, Inc.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5288-d24c17a55aeaed8bb04f68e5c211f9806cb559373f226d31482589800da2e7553</citedby><cites>FETCH-LOGICAL-c5288-d24c17a55aeaed8bb04f68e5c211f9806cb559373f226d31482589800da2e7553</cites><orcidid>0000-0002-1034-606X ; 0000-0001-9045-2139 ; 0000-0003-4271-9104</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fhipo.22408$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fhipo.22408$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25545461$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02064373$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Puighermanal, Emma</creatorcontrib><creatorcontrib>Biever, Anne</creatorcontrib><creatorcontrib>Espallergues, Julie</creatorcontrib><creatorcontrib>Gangarossa, Giuseppe</creatorcontrib><creatorcontrib>De Bundel, Dimitri</creatorcontrib><creatorcontrib>Valjent, Emmanuel</creatorcontrib><title>drd2-cre:ribotag mouse line unravels the possible diversity of dopamine d2 receptor-expressing cells of the dorsal mouse hippocampus</title><title>Hippocampus</title><addtitle>Hippocampus</addtitle><description>ABSTRACT
Increasing evidences suggest that dopamine facilitates the encoding of novel memories by the hippocampus. However, the role of dopamine D2 receptors (D2R) in such regulations remains elusive due to the lack of the precise identification of hippocampal D2R‐expressing cells. To address this issue, mice expressing the ribosomal protein Rpl22 tagged with the hemagglutinin (HA) epitope were crossed with Drd2‐Cre mice allowing the selective expression of HA in D2R‐containing cells (Drd2‐Cre:RiboTag mice). This new transgenic model revealed a more widespread pattern of D2R‐expressing cells identified by HA immunoreactivity than the one initially reported in Drd2‐EGFP mice, in which the hilar mossy cells were the main neuronal population detectable. In Drd2‐Cre:RiboTag mice, scattered HA/GAD67‐positive neurons were detected throughout the CA1/CA3 subfields, being preferentially localized in stratum oriens and stratum lacunosum‐moleculare. At the cellular level, HA‐labeled cells located in CA1/CA3 subfields co‐localized with calcium‐binding proteins (parvalbumin, calbindin, and calretinin), neuropeptides (neuropeptide Y, somatostatin), and other markers (neuronal nitric oxide synthase, mGluR1α, reelin, coupTFII, and potassium channel‐interacting protein 1). These results suggest that in addition to the glutamatergic hilar mossy cells, D2R‐expressing cells constitute a subpopulation of GABAergic hippocampal interneurons. © 2014 Wiley Periodicals, Inc.</description><subject>Animals</subject><subject>Calbindin 2 - metabolism</subject><subject>Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism</subject><subject>Channelrhodopsins</subject><subject>dopamine D2 receptor</subject><subject>Gene Expression Regulation - genetics</subject><subject>Glutamate Decarboxylase - metabolism</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>hilar mossy cells</subject><subject>Hippocampus - cytology</subject><subject>interneurons</subject><subject>KChIP1</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Microscopy, Confocal</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurons - metabolism</subject><subject>Receptors, Dopamine D2 - genetics</subject><subject>Receptors, Dopamine D2 - metabolism</subject><subject>Ribosomal Proteins - genetics</subject><subject>Ribosomal Proteins - metabolism</subject><subject>RiboTag mice</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><issn>1050-9631</issn><issn>1098-1063</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxSMEoqVw4QMgS1wAKWXs2E6WW1XRbqWlRfwREhfLiSddlyQOdrJ073xwnGa7Bw5w8mj0m-c385LkOYVjCsDerm3vjhnjUDxIDiksipSCzB5OtYB0ITN6kDwJ4QaAUgHwODlgQnDBJT1MfhtvWFp5fOdt6QZ9TVo3BiSN7ZCMndcbbAIZ1kh6F4ItGyTGbtAHO2yJq4lxvW4n1jDiscJ-cD7F295jpLtrUmET5yM4SRjng252P0TXvat024_hafKo1k3AZ7v3KPl69v7L6TJdXZ1fnJ6s0kqwokgN4xXNtRAaNZqiLIHXskBRMUrrRQGyKoVYZHlWMyZNRnnBRBH7YDTDXIjsKHk96651o3pvW-23ymmrlicrNfWAgeRRYEMj-2pme-9-jhgG1dowbaM7jP4VzYHyPOcc_o_KBUgB8eQRffkXeuNG38WlIxW9SsnvqDczVfl4dI_13iwFNUWupsjVXeQRfrGTHMsWzR69zzgCdAZ-2Qa3_5BSy4uPV_ei6Txjw4C3-xntfyiZZ7lQ3y7PleAfvn9mny7VWfYHE9rFBA</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Puighermanal, Emma</creator><creator>Biever, Anne</creator><creator>Espallergues, Julie</creator><creator>Gangarossa, Giuseppe</creator><creator>De Bundel, Dimitri</creator><creator>Valjent, Emmanuel</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><general>Wiley</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7TK</scope><scope>K9.</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-1034-606X</orcidid><orcidid>https://orcid.org/0000-0001-9045-2139</orcidid><orcidid>https://orcid.org/0000-0003-4271-9104</orcidid></search><sort><creationdate>201507</creationdate><title>drd2-cre:ribotag mouse line unravels the possible diversity of dopamine d2 receptor-expressing cells of the dorsal mouse hippocampus</title><author>Puighermanal, Emma ; Biever, Anne ; Espallergues, Julie ; Gangarossa, Giuseppe ; De Bundel, Dimitri ; Valjent, Emmanuel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5288-d24c17a55aeaed8bb04f68e5c211f9806cb559373f226d31482589800da2e7553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Calbindin 2 - metabolism</topic><topic>Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism</topic><topic>Channelrhodopsins</topic><topic>dopamine D2 receptor</topic><topic>Gene Expression Regulation - genetics</topic><topic>Glutamate Decarboxylase - metabolism</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>hilar mossy cells</topic><topic>Hippocampus - cytology</topic><topic>interneurons</topic><topic>KChIP1</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Microscopy, Confocal</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neurons - metabolism</topic><topic>Receptors, Dopamine D2 - genetics</topic><topic>Receptors, Dopamine D2 - metabolism</topic><topic>Ribosomal Proteins - genetics</topic><topic>Ribosomal Proteins - metabolism</topic><topic>RiboTag mice</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Puighermanal, Emma</creatorcontrib><creatorcontrib>Biever, Anne</creatorcontrib><creatorcontrib>Espallergues, Julie</creatorcontrib><creatorcontrib>Gangarossa, Giuseppe</creatorcontrib><creatorcontrib>De Bundel, Dimitri</creatorcontrib><creatorcontrib>Valjent, Emmanuel</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Hippocampus</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Puighermanal, Emma</au><au>Biever, Anne</au><au>Espallergues, Julie</au><au>Gangarossa, Giuseppe</au><au>De Bundel, Dimitri</au><au>Valjent, Emmanuel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>drd2-cre:ribotag mouse line unravels the possible diversity of dopamine d2 receptor-expressing cells of the dorsal mouse hippocampus</atitle><jtitle>Hippocampus</jtitle><addtitle>Hippocampus</addtitle><date>2015-07</date><risdate>2015</risdate><volume>25</volume><issue>7</issue><spage>858</spage><epage>875</epage><pages>858-875</pages><issn>1050-9631</issn><eissn>1098-1063</eissn><coden>HIPPEL</coden><abstract>ABSTRACT
Increasing evidences suggest that dopamine facilitates the encoding of novel memories by the hippocampus. However, the role of dopamine D2 receptors (D2R) in such regulations remains elusive due to the lack of the precise identification of hippocampal D2R‐expressing cells. To address this issue, mice expressing the ribosomal protein Rpl22 tagged with the hemagglutinin (HA) epitope were crossed with Drd2‐Cre mice allowing the selective expression of HA in D2R‐containing cells (Drd2‐Cre:RiboTag mice). This new transgenic model revealed a more widespread pattern of D2R‐expressing cells identified by HA immunoreactivity than the one initially reported in Drd2‐EGFP mice, in which the hilar mossy cells were the main neuronal population detectable. In Drd2‐Cre:RiboTag mice, scattered HA/GAD67‐positive neurons were detected throughout the CA1/CA3 subfields, being preferentially localized in stratum oriens and stratum lacunosum‐moleculare. At the cellular level, HA‐labeled cells located in CA1/CA3 subfields co‐localized with calcium‐binding proteins (parvalbumin, calbindin, and calretinin), neuropeptides (neuropeptide Y, somatostatin), and other markers (neuronal nitric oxide synthase, mGluR1α, reelin, coupTFII, and potassium channel‐interacting protein 1). These results suggest that in addition to the glutamatergic hilar mossy cells, D2R‐expressing cells constitute a subpopulation of GABAergic hippocampal interneurons. © 2014 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>25545461</pmid><doi>10.1002/hipo.22408</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0002-1034-606X</orcidid><orcidid>https://orcid.org/0000-0001-9045-2139</orcidid><orcidid>https://orcid.org/0000-0003-4271-9104</orcidid></addata></record> |
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| subjects | Animals Calbindin 2 - metabolism Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism Channelrhodopsins dopamine D2 receptor Gene Expression Regulation - genetics Glutamate Decarboxylase - metabolism Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism hilar mossy cells Hippocampus - cytology interneurons KChIP1 Life Sciences Male Membrane Proteins - metabolism Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Confocal Nerve Tissue Proteins - metabolism Neurons - metabolism Receptors, Dopamine D2 - genetics Receptors, Dopamine D2 - metabolism Ribosomal Proteins - genetics Ribosomal Proteins - metabolism RiboTag mice RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism |
| title | drd2-cre:ribotag mouse line unravels the possible diversity of dopamine d2 receptor-expressing cells of the dorsal mouse hippocampus |
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