Comparison between measurements of elasticity and free amino group content of ovalbumin microcapsule membranes: Discrimination of the cross-linking degree

The inverse correlation found between the shear modulus ( G s ) and free amino group (NH 2) content proves that the mechanical properties of ovalbumin microcapsules are governed by the reticulation process. [Display omitted] ► Comparison of mechanical and chemical characterization of ovalbumin capsules. ► Mechanical properties independent of pH for small reticulation times (pH ⩽ 7.4). ► The shear modulus increases with the time of reticulation. ► Inverse method for mechanical characterization discriminates degrees of reticulation. An inverse method is used to characterize the membrane mechanical behavior of liquid filled microcapsules. Cross-linked ovalbumin microcapsules are flowed and deformed into a cylindrical microchannel of comparable size. The deformed shape is compared to predictions obtained numerically when modeling a capsule under the same flow conditions. The unknown shear modulus value corresponds to the best fit. The degree of reticulation is estimated in parallel by determining the free amino groups remaining on the microcapsules after the cross-linking reaction. We characterize microcapsule populations fabricated at different reaction pH (5–8) and times (5–30 min) to study different cross-linking degrees. The capsule shear modulus and the amino groups are nearly constant with the reaction pH for the capsules fabricated after 5 min of reticulation. The shear modulus increases with the reaction time, while the NH 2 content decreases with it. A global increase in shear modulus with pH is also observed, together with an unexpected increase in NH 2 content. The study shows that the inverse method is capable of discriminating between various cross-linking degrees of microcapsules. Moreover, for this type of microcapsules, the mechanical method appears more reliable than the chemical one to obtain an estimation of their cross-linking degree.