The effect of gamma-ray irradiation on the Mn(ii) speciation in Deinococcus radiodurans and the potential role of Mn(ii)-orthophosphates
D. radiodurans accumulates large quantities of Mn( ii ), which is believed to form low molecular weight complexes with phosphate and metabolites that protect D. radiodurans from radiation damage. The concentration of Mn( ii ) species in D. radiodurans during the exponential and stationary phase was...
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| creator | Bruch, E. M de Groot, A Un, S Tabares, L. C |
| description | D. radiodurans
accumulates large quantities of Mn(
ii
), which is believed to form low molecular weight complexes with phosphate and metabolites that protect
D. radiodurans
from radiation damage. The concentration of Mn(
ii
) species in
D. radiodurans
during the exponential and stationary phase was determined using high-field EPR and biochemical techniques. In the exponential growth phase cells a large fraction of the manganese was in the form of Mn(
ii
)-orthophosphate complexes. By contrast, the intracellular concentration of these compounds in stationary phase cells was less than 16 μM, while that of Mn superoxide dismutase was 320 μM and that of another, yet unidentified, Mn(
ii
) protein was 250 μM. Stationary cells were found to be equally resistant to irradiation as the exponential cells in spite of having significant lower Mn(
ii
)-orthophosphate concentrations. Gamma irradiation induced no changes in the Mn(
ii
) speciation. During stationary growth phase
D. radiodurans
favours the production of the two Mn-proteins over low molecular weight complexes suggesting that the latter were not essential for radio-resistance at this stage of growth.
The intracellular concentration of Mn-phosphates does not correlate with radioresistance. |
| doi_str_mv | 10.1039/c5mt00009b |
| format | Article |
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accumulates large quantities of Mn(
ii
), which is believed to form low molecular weight complexes with phosphate and metabolites that protect
D. radiodurans
from radiation damage. The concentration of Mn(
ii
) species in
D. radiodurans
during the exponential and stationary phase was determined using high-field EPR and biochemical techniques. In the exponential growth phase cells a large fraction of the manganese was in the form of Mn(
ii
)-orthophosphate complexes. By contrast, the intracellular concentration of these compounds in stationary phase cells was less than 16 μM, while that of Mn superoxide dismutase was 320 μM and that of another, yet unidentified, Mn(
ii
) protein was 250 μM. Stationary cells were found to be equally resistant to irradiation as the exponential cells in spite of having significant lower Mn(
ii
)-orthophosphate concentrations. Gamma irradiation induced no changes in the Mn(
ii
) speciation. During stationary growth phase
D. radiodurans
favours the production of the two Mn-proteins over low molecular weight complexes suggesting that the latter were not essential for radio-resistance at this stage of growth.
The intracellular concentration of Mn-phosphates does not correlate with radioresistance.</description><identifier>ISSN: 1756-5901</identifier><identifier>EISSN: 1756-591X</identifier><identifier>DOI: 10.1039/c5mt00009b</identifier><identifier>PMID: 25811292</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Deinococcus - chemistry ; Deinococcus - growth & development ; Deinococcus - metabolism ; Deinococcus - radiation effects ; Electron Spin Resonance Spectroscopy ; Gamma Rays ; Life Sciences ; Manganese - analysis ; Manganese - metabolism ; Phosphates - analysis ; Phosphates - metabolism</subject><ispartof>Metallomics, 2015-05, Vol.7 (5), p.98-916</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-811a6192a47ddce96b55a13ded94501d3eab52d144b63a44d7c7722da83013083</citedby><cites>FETCH-LOGICAL-c405t-811a6192a47ddce96b55a13ded94501d3eab52d144b63a44d7c7722da83013083</cites><orcidid>0000-0003-2202-5279</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25811292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02014055$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Bruch, E. M</creatorcontrib><creatorcontrib>de Groot, A</creatorcontrib><creatorcontrib>Un, S</creatorcontrib><creatorcontrib>Tabares, L. C</creatorcontrib><title>The effect of gamma-ray irradiation on the Mn(ii) speciation in Deinococcus radiodurans and the potential role of Mn(ii)-orthophosphates</title><title>Metallomics</title><addtitle>Metallomics</addtitle><description>D. radiodurans
accumulates large quantities of Mn(
ii
), which is believed to form low molecular weight complexes with phosphate and metabolites that protect
D. radiodurans
from radiation damage. The concentration of Mn(
ii
) species in
D. radiodurans
during the exponential and stationary phase was determined using high-field EPR and biochemical techniques. In the exponential growth phase cells a large fraction of the manganese was in the form of Mn(
ii
)-orthophosphate complexes. By contrast, the intracellular concentration of these compounds in stationary phase cells was less than 16 μM, while that of Mn superoxide dismutase was 320 μM and that of another, yet unidentified, Mn(
ii
) protein was 250 μM. Stationary cells were found to be equally resistant to irradiation as the exponential cells in spite of having significant lower Mn(
ii
)-orthophosphate concentrations. Gamma irradiation induced no changes in the Mn(
ii
) speciation. During stationary growth phase
D. radiodurans
favours the production of the two Mn-proteins over low molecular weight complexes suggesting that the latter were not essential for radio-resistance at this stage of growth.
The intracellular concentration of Mn-phosphates does not correlate with radioresistance.</description><subject>Deinococcus - chemistry</subject><subject>Deinococcus - growth & development</subject><subject>Deinococcus - metabolism</subject><subject>Deinococcus - radiation effects</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Gamma Rays</subject><subject>Life Sciences</subject><subject>Manganese - analysis</subject><subject>Manganese - metabolism</subject><subject>Phosphates - analysis</subject><subject>Phosphates - metabolism</subject><issn>1756-5901</issn><issn>1756-591X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxS0EoqVw4Q4ytxYp4LHjZHMsy58ibcVlkbhZE9shRkkcbAep34CPjbdZlhuWJY88v_dmpEfIc2BvgInmrZZjYvk07QNyDrWsCtnAt4enmsEZeRLjD8aqkjH5mJxxuQHgDT8nv_e9pbbrrE7Ud_Q7jiMWAe-oCwGNw-T8RPNNGbudLp27onG2-thwE31v3eS113qJ9KDwZgk4RYqTuRfNPtkpORxo8IM9zFhtCh9S7-fex7nHZONT8qjDIdpnx_eCfP34Yb-9KXZfPn3eXu8KXTKZirw3VtBwLGtjtG2qVkoEYaxpSsnACIut5AbKsq0ElqWpdV1zbnAjGAi2ERfkavXtcVBzcCOGO-XRqZvrnTr8Mc4gj5K_ILOXKzsH_3OxManRRW2HASfrl6ig2gCvhGA8o69XVAcfY7DdyRuYOqSktvJ2f5_Suwy_PPou7WjNCf0bSwZerUCI-tT9F7OaTZeZF_9jxB940KIa</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Bruch, E. M</creator><creator>de Groot, A</creator><creator>Un, S</creator><creator>Tabares, L. C</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-2202-5279</orcidid></search><sort><creationdate>20150501</creationdate><title>The effect of gamma-ray irradiation on the Mn(ii) speciation in Deinococcus radiodurans and the potential role of Mn(ii)-orthophosphates</title><author>Bruch, E. M ; de Groot, A ; Un, S ; Tabares, L. C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-811a6192a47ddce96b55a13ded94501d3eab52d144b63a44d7c7722da83013083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Deinococcus - chemistry</topic><topic>Deinococcus - growth & development</topic><topic>Deinococcus - metabolism</topic><topic>Deinococcus - radiation effects</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>Gamma Rays</topic><topic>Life Sciences</topic><topic>Manganese - analysis</topic><topic>Manganese - metabolism</topic><topic>Phosphates - analysis</topic><topic>Phosphates - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bruch, E. M</creatorcontrib><creatorcontrib>de Groot, A</creatorcontrib><creatorcontrib>Un, S</creatorcontrib><creatorcontrib>Tabares, L. C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Metallomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bruch, E. M</au><au>de Groot, A</au><au>Un, S</au><au>Tabares, L. C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of gamma-ray irradiation on the Mn(ii) speciation in Deinococcus radiodurans and the potential role of Mn(ii)-orthophosphates</atitle><jtitle>Metallomics</jtitle><addtitle>Metallomics</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>7</volume><issue>5</issue><spage>98</spage><epage>916</epage><pages>98-916</pages><issn>1756-5901</issn><eissn>1756-591X</eissn><abstract>D. radiodurans
accumulates large quantities of Mn(
ii
), which is believed to form low molecular weight complexes with phosphate and metabolites that protect
D. radiodurans
from radiation damage. The concentration of Mn(
ii
) species in
D. radiodurans
during the exponential and stationary phase was determined using high-field EPR and biochemical techniques. In the exponential growth phase cells a large fraction of the manganese was in the form of Mn(
ii
)-orthophosphate complexes. By contrast, the intracellular concentration of these compounds in stationary phase cells was less than 16 μM, while that of Mn superoxide dismutase was 320 μM and that of another, yet unidentified, Mn(
ii
) protein was 250 μM. Stationary cells were found to be equally resistant to irradiation as the exponential cells in spite of having significant lower Mn(
ii
)-orthophosphate concentrations. Gamma irradiation induced no changes in the Mn(
ii
) speciation. During stationary growth phase
D. radiodurans
favours the production of the two Mn-proteins over low molecular weight complexes suggesting that the latter were not essential for radio-resistance at this stage of growth.
The intracellular concentration of Mn-phosphates does not correlate with radioresistance.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>25811292</pmid><doi>10.1039/c5mt00009b</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-2202-5279</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1756-5901 |
| ispartof | Metallomics, 2015-05, Vol.7 (5), p.98-916 |
| issn | 1756-5901 1756-591X |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02014055v1 |
| source | MEDLINE; Royal Society Of Chemistry Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Deinococcus - chemistry Deinococcus - growth & development Deinococcus - metabolism Deinococcus - radiation effects Electron Spin Resonance Spectroscopy Gamma Rays Life Sciences Manganese - analysis Manganese - metabolism Phosphates - analysis Phosphates - metabolism |
| title | The effect of gamma-ray irradiation on the Mn(ii) speciation in Deinococcus radiodurans and the potential role of Mn(ii)-orthophosphates |
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