Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix

We developed a competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines in fish samples. MIPs synthesized by precipitation polymerization using histamine as template were used in a batch binding assay analogous to competitive fluoroimmunoassays. Introducing a compl...

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Veröffentlicht in:Talanta (Oxford) 2018-05, Vol.181, p.190-196
Hauptverfasser: Mattsson, Leena, Xu, Jingjing, Preininger, Claudia, Tse Sum Bui, Bernadette, Haupt, Karsten
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container_start_page 190
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creator Mattsson, Leena
Xu, Jingjing
Preininger, Claudia
Tse Sum Bui, Bernadette
Haupt, Karsten
description We developed a competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines in fish samples. MIPs synthesized by precipitation polymerization using histamine as template were used in a batch binding assay analogous to competitive fluoroimmunoassays. Introducing a complex sample matrix, such as fish extract, into the assay changes the environment and the binding conditions, therefore the importance of the sample preparation is extensively discussed. Several extraction and purification methods for fish were comprehensively studied, and an optimal clean-up procedure for fish samples using liquid-liquid extraction was developed. The feasibility of the competitive MIP assay was shown in the purified fish extract over a broad histamine range (1 − 430µM). The MIP had the highest affinity towards histamine, but recognized also the structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine, providing simultaneous analysis and assessment of the total amount of biogenic amines. [Display omitted] •A competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines was developed.•Various sample preparation methods for fish were assessed for their effect in assay performance.•Liquid-liquid extraction method was developed to enable the binding of histamine to the molecularly imprinted polymer in the sample matrix.•The MIP had the highest affinity towards histamine, but recognized also structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine.
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MIPs synthesized by precipitation polymerization using histamine as template were used in a batch binding assay analogous to competitive fluoroimmunoassays. Introducing a complex sample matrix, such as fish extract, into the assay changes the environment and the binding conditions, therefore the importance of the sample preparation is extensively discussed. Several extraction and purification methods for fish were comprehensively studied, and an optimal clean-up procedure for fish samples using liquid-liquid extraction was developed. The feasibility of the competitive MIP assay was shown in the purified fish extract over a broad histamine range (1 − 430µM). The MIP had the highest affinity towards histamine, but recognized also the structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine, providing simultaneous analysis and assessment of the total amount of biogenic amines. [Display omitted] •A competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines was developed.•Various sample preparation methods for fish were assessed for their effect in assay performance.•Liquid-liquid extraction method was developed to enable the binding of histamine to the molecularly imprinted polymer in the sample matrix.•The MIP had the highest affinity towards histamine, but recognized also structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2018.01.010</identifier><identifier>PMID: 29426500</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Biogenic amines ; Chemical Sciences ; Environmental Sciences ; Fluorescence detection ; Histamine ; Liquid – liquid extraction ; Molecularly imprinted polymer ; Polymers ; Sample preparation</subject><ispartof>Talanta (Oxford), 2018-05, Vol.181, p.190-196</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. 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subjects Biogenic amines
Chemical Sciences
Environmental Sciences
Fluorescence detection
Histamine
Liquid – liquid extraction
Molecularly imprinted polymer
Polymers
Sample preparation
title Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix
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