Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix
We developed a competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines in fish samples. MIPs synthesized by precipitation polymerization using histamine as template were used in a batch binding assay analogous to competitive fluoroimmunoassays. Introducing a compl...
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Veröffentlicht in: | Talanta (Oxford) 2018-05, Vol.181, p.190-196 |
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creator | Mattsson, Leena Xu, Jingjing Preininger, Claudia Tse Sum Bui, Bernadette Haupt, Karsten |
description | We developed a competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines in fish samples. MIPs synthesized by precipitation polymerization using histamine as template were used in a batch binding assay analogous to competitive fluoroimmunoassays. Introducing a complex sample matrix, such as fish extract, into the assay changes the environment and the binding conditions, therefore the importance of the sample preparation is extensively discussed. Several extraction and purification methods for fish were comprehensively studied, and an optimal clean-up procedure for fish samples using liquid-liquid extraction was developed. The feasibility of the competitive MIP assay was shown in the purified fish extract over a broad histamine range (1 − 430µM). The MIP had the highest affinity towards histamine, but recognized also the structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine, providing simultaneous analysis and assessment of the total amount of biogenic amines.
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•A competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines was developed.•Various sample preparation methods for fish were assessed for their effect in assay performance.•Liquid-liquid extraction method was developed to enable the binding of histamine to the molecularly imprinted polymer in the sample matrix.•The MIP had the highest affinity towards histamine, but recognized also structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine. |
doi_str_mv | 10.1016/j.talanta.2018.01.010 |
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[Display omitted]
•A competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines was developed.•Various sample preparation methods for fish were assessed for their effect in assay performance.•Liquid-liquid extraction method was developed to enable the binding of histamine to the molecularly imprinted polymer in the sample matrix.•The MIP had the highest affinity towards histamine, but recognized also structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2018.01.010</identifier><identifier>PMID: 29426500</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Biogenic amines ; Chemical Sciences ; Environmental Sciences ; Fluorescence detection ; Histamine ; Liquid – liquid extraction ; Molecularly imprinted polymer ; Polymers ; Sample preparation</subject><ispartof>Talanta (Oxford), 2018-05, Vol.181, p.190-196</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-c06489523086e0dd944f3d747fc0fff97fcd88c9333c779b757e7d27d740b9113</citedby><cites>FETCH-LOGICAL-c399t-c06489523086e0dd944f3d747fc0fff97fcd88c9333c779b757e7d27d740b9113</cites><orcidid>0000-0002-4170-2303 ; 0000-0001-6777-2919 ; 0000-0001-6743-5066</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2018.01.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29426500$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://utc.hal.science/hal-01988852$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Mattsson, Leena</creatorcontrib><creatorcontrib>Xu, Jingjing</creatorcontrib><creatorcontrib>Preininger, Claudia</creatorcontrib><creatorcontrib>Tse Sum Bui, Bernadette</creatorcontrib><creatorcontrib>Haupt, Karsten</creatorcontrib><title>Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>We developed a competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines in fish samples. MIPs synthesized by precipitation polymerization using histamine as template were used in a batch binding assay analogous to competitive fluoroimmunoassays. Introducing a complex sample matrix, such as fish extract, into the assay changes the environment and the binding conditions, therefore the importance of the sample preparation is extensively discussed. Several extraction and purification methods for fish were comprehensively studied, and an optimal clean-up procedure for fish samples using liquid-liquid extraction was developed. The feasibility of the competitive MIP assay was shown in the purified fish extract over a broad histamine range (1 − 430µM). The MIP had the highest affinity towards histamine, but recognized also the structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine, providing simultaneous analysis and assessment of the total amount of biogenic amines.
[Display omitted]
•A competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines was developed.•Various sample preparation methods for fish were assessed for their effect in assay performance.•Liquid-liquid extraction method was developed to enable the binding of histamine to the molecularly imprinted polymer in the sample matrix.•The MIP had the highest affinity towards histamine, but recognized also structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine.</description><subject>Biogenic amines</subject><subject>Chemical Sciences</subject><subject>Environmental Sciences</subject><subject>Fluorescence detection</subject><subject>Histamine</subject><subject>Liquid – liquid extraction</subject><subject>Molecularly imprinted polymer</subject><subject>Polymers</subject><subject>Sample preparation</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqFkcGO0zAQhiMEYsvCI4B8hEPKOE6a-IRW1cIiVeICZ8u1x1tXdhxsp9q-AY-No5a9Io00kvXN_OP_r6r3FNYU6ObzcZ2lk2OW6wbosAZaCl5UKzr0rGZdz15WKwDGa05buKnepHQEgIYBe13dNLxtNh3AqvqzDX7CbLM9ITFuDhGTwjGTKeGsQ229n8cgU5Jngk-TC4UcH4kPDtXsZHRnYv0U7ZhRkym4s8eYiAmR5AMSjRlVtmEkwZC9DY84WkWktyMmYkdibDoQL3O0T2-rV0a6hO-u_bb69fX-5_ah3v349n17t6sV4zzXCjbtwLvyj2GDoDVvW8N03_ZGgTGGl66HQXHGmOp7vu-7Hnvd9AWBPaeU3VafLnsP0olyuJfxLIK04uFuJ5Y3oHwYhq45LezHCzvF8HvGlIW3xR1XfMcwJ9FA8XxT6AXtLqiKIaWI5nk3BbEEJo7iGphYAisypaDMfbhKzHuP-nnqX0IF-HIBsJhyshhFUhZHhdrG4q3Qwf5H4i8Fpqxo</recordid><startdate>20180501</startdate><enddate>20180501</enddate><creator>Mattsson, Leena</creator><creator>Xu, Jingjing</creator><creator>Preininger, Claudia</creator><creator>Tse Sum Bui, Bernadette</creator><creator>Haupt, Karsten</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-4170-2303</orcidid><orcidid>https://orcid.org/0000-0001-6777-2919</orcidid><orcidid>https://orcid.org/0000-0001-6743-5066</orcidid></search><sort><creationdate>20180501</creationdate><title>Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix</title><author>Mattsson, Leena ; Xu, Jingjing ; Preininger, Claudia ; Tse Sum Bui, Bernadette ; Haupt, Karsten</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-c06489523086e0dd944f3d747fc0fff97fcd88c9333c779b757e7d27d740b9113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biogenic amines</topic><topic>Chemical Sciences</topic><topic>Environmental Sciences</topic><topic>Fluorescence detection</topic><topic>Histamine</topic><topic>Liquid – liquid extraction</topic><topic>Molecularly imprinted polymer</topic><topic>Polymers</topic><topic>Sample preparation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mattsson, Leena</creatorcontrib><creatorcontrib>Xu, Jingjing</creatorcontrib><creatorcontrib>Preininger, Claudia</creatorcontrib><creatorcontrib>Tse Sum Bui, Bernadette</creatorcontrib><creatorcontrib>Haupt, Karsten</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mattsson, Leena</au><au>Xu, Jingjing</au><au>Preininger, Claudia</au><au>Tse Sum Bui, Bernadette</au><au>Haupt, Karsten</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2018-05-01</date><risdate>2018</risdate><volume>181</volume><spage>190</spage><epage>196</epage><pages>190-196</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><abstract>We developed a competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines in fish samples. MIPs synthesized by precipitation polymerization using histamine as template were used in a batch binding assay analogous to competitive fluoroimmunoassays. Introducing a complex sample matrix, such as fish extract, into the assay changes the environment and the binding conditions, therefore the importance of the sample preparation is extensively discussed. Several extraction and purification methods for fish were comprehensively studied, and an optimal clean-up procedure for fish samples using liquid-liquid extraction was developed. The feasibility of the competitive MIP assay was shown in the purified fish extract over a broad histamine range (1 − 430µM). The MIP had the highest affinity towards histamine, but recognized also the structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine, providing simultaneous analysis and assessment of the total amount of biogenic amines.
[Display omitted]
•A competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines was developed.•Various sample preparation methods for fish were assessed for their effect in assay performance.•Liquid-liquid extraction method was developed to enable the binding of histamine to the molecularly imprinted polymer in the sample matrix.•The MIP had the highest affinity towards histamine, but recognized also structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>29426500</pmid><doi>10.1016/j.talanta.2018.01.010</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-4170-2303</orcidid><orcidid>https://orcid.org/0000-0001-6777-2919</orcidid><orcidid>https://orcid.org/0000-0001-6743-5066</orcidid></addata></record> |
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subjects | Biogenic amines Chemical Sciences Environmental Sciences Fluorescence detection Histamine Liquid – liquid extraction Molecularly imprinted polymer Polymers Sample preparation |
title | Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix |
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