Receptors are affected by selection with each Bacillus thuringiensis israelensis Cry toxin but not with the full Bti mixture in Aedes aegypti
Bacillus thuringiensis israelensis (Bti) toxins are increasingly used for mosquito control, but little is known about the precise mode of action of each of these toxins, and how they interact to kill mosquito larvae. By using RNA sequencing, we investigated change in gene transcription level and pol...
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| Veröffentlicht in: | Infection, genetics and evolution genetics and evolution, 2016-10, Vol.44, p.218-227 |
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| creator | Stalinski, Renaud Laporte, Frederic Tetreau, Guillaume Després, Laurence |
| description | Bacillus thuringiensis israelensis (Bti) toxins are increasingly used for mosquito control, but little is known about the precise mode of action of each of these toxins, and how they interact to kill mosquito larvae. By using RNA sequencing, we investigated change in gene transcription level and polymorphism variations associated with resistance to each Bti Cry toxin and to the full Bti toxin mixture in the dengue vector Aedes aegypti. The up-regulation of genes related to chitin metabolism in all selected strain suggests a generalist, non-toxin-specific response to Bti selection in Aedes aegypti. Changes in the transcription level and/or protein sequences of several putative Cry toxin receptors (APNs, ALPs, α-amylases, glucoside hydrolases, ABC transporters) were specific to each Cry toxin. Selective sweeps associated with Cry4Aa resistance were detected in 2 ALP and 1 APN genes. The lack of selection of toxin-specific receptors in the Bti-selected strain supports the hypothesis that Cyt toxin acts as a receptor for Cry toxins in mosquitoes.
[Display omitted]
•Genes encoding for chitin metabolism are upregulated in all selected strains.•Protoxin processing is not altered in Cry-selected mosquito larvae.•Toxin-specific modifications in the transcriptional profiles and in polymorphisms of receptors are selected by Bti Cry toxins.•Selective sweeps associated with Cry4Aa resistance were detected in 2 ALP and 1 APN genes. |
| doi_str_mv | 10.1016/j.meegid.2016.07.009 |
| format | Article |
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[Display omitted]
•Genes encoding for chitin metabolism are upregulated in all selected strains.•Protoxin processing is not altered in Cry-selected mosquito larvae.•Toxin-specific modifications in the transcriptional profiles and in polymorphisms of receptors are selected by Bti Cry toxins.•Selective sweeps associated with Cry4Aa resistance were detected in 2 ALP and 1 APN genes.</description><identifier>ISSN: 1567-1348</identifier><identifier>EISSN: 1567-7257</identifier><identifier>DOI: 10.1016/j.meegid.2016.07.009</identifier><identifier>PMID: 27418233</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aedes - drug effects ; Aedes - genetics ; Alkaline Phosphatase - genetics ; Animals ; Bacillus thuringiensis israelensis toxins ; Bacterial Proteins - pharmacology ; Bio-insecticide resistance ; Biochemistry, Molecular Biology ; CD13 Antigens - genetics ; Chitin - genetics ; Chitin - metabolism ; Ecology, environment ; Endotoxins - pharmacology ; Gene Expression Regulation - drug effects ; Genomics ; Hemolysin Proteins - pharmacology ; Insect Proteins - genetics ; Insecticide Resistance - drug effects ; Insecticide Resistance - genetics ; Larva - drug effects ; Larva - genetics ; Life Sciences ; Mosquito ; Mosquito Vectors - genetics ; Polymorphism, Single Nucleotide ; Receptors, Cell Surface ; RNA-seq ; Single Nucleotide Polymorphism ; Specific receptors ; Symbiosis</subject><ispartof>Infection, genetics and evolution, 2016-10, Vol.44, p.218-227</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-1c1c9916f19f6d0c9a15c8633b73b1978450c5f05ee6d8ef599cb031880713f83</citedby><cites>FETCH-LOGICAL-c396t-1c1c9916f19f6d0c9a15c8633b73b1978450c5f05ee6d8ef599cb031880713f83</cites><orcidid>0000-0003-3360-4786</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.meegid.2016.07.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27418233$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01988449$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Stalinski, Renaud</creatorcontrib><creatorcontrib>Laporte, Frederic</creatorcontrib><creatorcontrib>Tetreau, Guillaume</creatorcontrib><creatorcontrib>Després, Laurence</creatorcontrib><title>Receptors are affected by selection with each Bacillus thuringiensis israelensis Cry toxin but not with the full Bti mixture in Aedes aegypti</title><title>Infection, genetics and evolution</title><addtitle>Infect Genet Evol</addtitle><description>Bacillus thuringiensis israelensis (Bti) toxins are increasingly used for mosquito control, but little is known about the precise mode of action of each of these toxins, and how they interact to kill mosquito larvae. By using RNA sequencing, we investigated change in gene transcription level and polymorphism variations associated with resistance to each Bti Cry toxin and to the full Bti toxin mixture in the dengue vector Aedes aegypti. The up-regulation of genes related to chitin metabolism in all selected strain suggests a generalist, non-toxin-specific response to Bti selection in Aedes aegypti. Changes in the transcription level and/or protein sequences of several putative Cry toxin receptors (APNs, ALPs, α-amylases, glucoside hydrolases, ABC transporters) were specific to each Cry toxin. Selective sweeps associated with Cry4Aa resistance were detected in 2 ALP and 1 APN genes. The lack of selection of toxin-specific receptors in the Bti-selected strain supports the hypothesis that Cyt toxin acts as a receptor for Cry toxins in mosquitoes.
[Display omitted]
•Genes encoding for chitin metabolism are upregulated in all selected strains.•Protoxin processing is not altered in Cry-selected mosquito larvae.•Toxin-specific modifications in the transcriptional profiles and in polymorphisms of receptors are selected by Bti Cry toxins.•Selective sweeps associated with Cry4Aa resistance were detected in 2 ALP and 1 APN genes.</description><subject>Aedes - drug effects</subject><subject>Aedes - genetics</subject><subject>Alkaline Phosphatase - genetics</subject><subject>Animals</subject><subject>Bacillus thuringiensis israelensis toxins</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Bio-insecticide resistance</subject><subject>Biochemistry, Molecular Biology</subject><subject>CD13 Antigens - genetics</subject><subject>Chitin - genetics</subject><subject>Chitin - metabolism</subject><subject>Ecology, environment</subject><subject>Endotoxins - pharmacology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genomics</subject><subject>Hemolysin Proteins - pharmacology</subject><subject>Insect Proteins - genetics</subject><subject>Insecticide Resistance - drug effects</subject><subject>Insecticide Resistance - genetics</subject><subject>Larva - drug effects</subject><subject>Larva - genetics</subject><subject>Life Sciences</subject><subject>Mosquito</subject><subject>Mosquito Vectors - genetics</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Receptors, Cell Surface</subject><subject>RNA-seq</subject><subject>Single Nucleotide Polymorphism</subject><subject>Specific receptors</subject><subject>Symbiosis</subject><issn>1567-1348</issn><issn>1567-7257</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAURS0EoqXwBwh5CYsJdpzE9gZpOioUaSSkqqwtx3meeOQkg-2Uzkfwz_WQoUtWvn46913pXYTeU1JQQpvP-2IA2LmuKPOvILwgRL5Al7Ru-IqXNX951pRV4gK9iXFPCOWkFK_RRckrKkrGLtGfOzBwSFOIWAfA2lowCTrcHnEEn7WbRvzbpR6DNj2-1sZ5P0ec-jm4cedgjC5iF4PO9F-9CUecpkc34nZOeJzSYk89YDt7j6-Tw4N7THOOy9AaOsjZsDseknuLXlntI7w7v1fo59eb-83tavvj2_fNersyTDZpRQ01UtLGUmmbjhipaW1Ew1jLWUslF1VNTG1JDdB0AmwtpWkJo0IQTpkV7Ap9Wvb22qtDcIMORzVpp27XW3WaESqFqCr5QDP7cWEPYfo1Q0xqcNGA93qEaY4qH5LUshSyyWi1oCZMMQawz7spUafS1F4tpalTaYpwlUvLtg_nhLkdoHs2_WspA18WAPJNHhwEFU2-vIHOhVyR6ib3_4QnKdaq7A</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Stalinski, Renaud</creator><creator>Laporte, Frederic</creator><creator>Tetreau, Guillaume</creator><creator>Després, Laurence</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-3360-4786</orcidid></search><sort><creationdate>201610</creationdate><title>Receptors are affected by selection with each Bacillus thuringiensis israelensis Cry toxin but not with the full Bti mixture in Aedes aegypti</title><author>Stalinski, Renaud ; Laporte, Frederic ; Tetreau, Guillaume ; Després, Laurence</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-1c1c9916f19f6d0c9a15c8633b73b1978450c5f05ee6d8ef599cb031880713f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aedes - drug effects</topic><topic>Aedes - genetics</topic><topic>Alkaline Phosphatase - genetics</topic><topic>Animals</topic><topic>Bacillus thuringiensis israelensis toxins</topic><topic>Bacterial Proteins - pharmacology</topic><topic>Bio-insecticide resistance</topic><topic>Biochemistry, Molecular Biology</topic><topic>CD13 Antigens - genetics</topic><topic>Chitin - genetics</topic><topic>Chitin - metabolism</topic><topic>Ecology, environment</topic><topic>Endotoxins - pharmacology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Genomics</topic><topic>Hemolysin Proteins - pharmacology</topic><topic>Insect Proteins - genetics</topic><topic>Insecticide Resistance - drug effects</topic><topic>Insecticide Resistance - genetics</topic><topic>Larva - drug effects</topic><topic>Larva - genetics</topic><topic>Life Sciences</topic><topic>Mosquito</topic><topic>Mosquito Vectors - genetics</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Receptors, Cell Surface</topic><topic>RNA-seq</topic><topic>Single Nucleotide Polymorphism</topic><topic>Specific receptors</topic><topic>Symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stalinski, Renaud</creatorcontrib><creatorcontrib>Laporte, Frederic</creatorcontrib><creatorcontrib>Tetreau, Guillaume</creatorcontrib><creatorcontrib>Després, Laurence</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Infection, genetics and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stalinski, Renaud</au><au>Laporte, Frederic</au><au>Tetreau, Guillaume</au><au>Després, Laurence</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Receptors are affected by selection with each Bacillus thuringiensis israelensis Cry toxin but not with the full Bti mixture in Aedes aegypti</atitle><jtitle>Infection, genetics and evolution</jtitle><addtitle>Infect Genet Evol</addtitle><date>2016-10</date><risdate>2016</risdate><volume>44</volume><spage>218</spage><epage>227</epage><pages>218-227</pages><issn>1567-1348</issn><eissn>1567-7257</eissn><abstract>Bacillus thuringiensis israelensis (Bti) toxins are increasingly used for mosquito control, but little is known about the precise mode of action of each of these toxins, and how they interact to kill mosquito larvae. By using RNA sequencing, we investigated change in gene transcription level and polymorphism variations associated with resistance to each Bti Cry toxin and to the full Bti toxin mixture in the dengue vector Aedes aegypti. The up-regulation of genes related to chitin metabolism in all selected strain suggests a generalist, non-toxin-specific response to Bti selection in Aedes aegypti. Changes in the transcription level and/or protein sequences of several putative Cry toxin receptors (APNs, ALPs, α-amylases, glucoside hydrolases, ABC transporters) were specific to each Cry toxin. Selective sweeps associated with Cry4Aa resistance were detected in 2 ALP and 1 APN genes. The lack of selection of toxin-specific receptors in the Bti-selected strain supports the hypothesis that Cyt toxin acts as a receptor for Cry toxins in mosquitoes.
[Display omitted]
•Genes encoding for chitin metabolism are upregulated in all selected strains.•Protoxin processing is not altered in Cry-selected mosquito larvae.•Toxin-specific modifications in the transcriptional profiles and in polymorphisms of receptors are selected by Bti Cry toxins.•Selective sweeps associated with Cry4Aa resistance were detected in 2 ALP and 1 APN genes.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27418233</pmid><doi>10.1016/j.meegid.2016.07.009</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-3360-4786</orcidid></addata></record> |
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| subjects | Aedes - drug effects Aedes - genetics Alkaline Phosphatase - genetics Animals Bacillus thuringiensis israelensis toxins Bacterial Proteins - pharmacology Bio-insecticide resistance Biochemistry, Molecular Biology CD13 Antigens - genetics Chitin - genetics Chitin - metabolism Ecology, environment Endotoxins - pharmacology Gene Expression Regulation - drug effects Genomics Hemolysin Proteins - pharmacology Insect Proteins - genetics Insecticide Resistance - drug effects Insecticide Resistance - genetics Larva - drug effects Larva - genetics Life Sciences Mosquito Mosquito Vectors - genetics Polymorphism, Single Nucleotide Receptors, Cell Surface RNA-seq Single Nucleotide Polymorphism Specific receptors Symbiosis |
| title | Receptors are affected by selection with each Bacillus thuringiensis israelensis Cry toxin but not with the full Bti mixture in Aedes aegypti |
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