Phylogenetic diversity of Bradyrhizobium strains nodulating Calicotome spinosa in the Northeast of Algeria
Fifty-two slow-growing strains were isolated from root nodules of Calicotome spinosa grown in the Northeast of Algeria and grouped in 24 rep-PCR clusters. One representative strain for each profile was further phylogenetically characterized. The nearly complete 16S rRNA gene sequence indicated that...
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| Veröffentlicht in: | Systematic and applied microbiology 2018-09, Vol.41 (5), p.452-459 |
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| container_title | Systematic and applied microbiology |
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| creator | Salmi, Adouda Boulila, Farida Bourebaba, Yasmina Le Roux, Christine Belhadi, Djellali de Lajudie, Philippe |
| description | Fifty-two slow-growing strains were isolated from root nodules of Calicotome spinosa grown in the Northeast of Algeria and grouped in 24 rep-PCR clusters. One representative strain for each profile was further phylogenetically characterized. The nearly complete 16S rRNA gene sequence indicated that all strains were affiliated to Bradyrhizobium. Multi-Locus Sequence Analysis (MLSA) of the atpD, glnII and recA genes and of the 16S-23S rRNA internal transcribed spacer (ITS) showed that these strains formed four divergent clusters: one close to Bradyrhizobium canariense and Bradyrhizobium lupini and three others separate from all the described species, representing three putative new Bradyrhizobium species. A phylogenetic analysis based on the nodC gene sequence affiliated the strains to either of the two symbiovars, genistearum or retamae. |
| doi_str_mv | 10.1016/j.syapm.2018.05.005 |
| format | Article |
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One representative strain for each profile was further phylogenetically characterized. The nearly complete 16S rRNA gene sequence indicated that all strains were affiliated to Bradyrhizobium. Multi-Locus Sequence Analysis (MLSA) of the atpD, glnII and recA genes and of the 16S-23S rRNA internal transcribed spacer (ITS) showed that these strains formed four divergent clusters: one close to Bradyrhizobium canariense and Bradyrhizobium lupini and three others separate from all the described species, representing three putative new Bradyrhizobium species. 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All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-1be480a8dc001a7d1e2de3210cf32f574dfde37b4407be46e630820cdd3f03053</citedby><cites>FETCH-LOGICAL-c393t-1be480a8dc001a7d1e2de3210cf32f574dfde37b4407be46e630820cdd3f03053</cites><orcidid>0000-0002-2196-6352 ; 0000-0003-0668-3589</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.syapm.2018.05.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29891104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01956111$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Salmi, Adouda</creatorcontrib><creatorcontrib>Boulila, Farida</creatorcontrib><creatorcontrib>Bourebaba, Yasmina</creatorcontrib><creatorcontrib>Le Roux, Christine</creatorcontrib><creatorcontrib>Belhadi, Djellali</creatorcontrib><creatorcontrib>de Lajudie, Philippe</creatorcontrib><title>Phylogenetic diversity of Bradyrhizobium strains nodulating Calicotome spinosa in the Northeast of Algeria</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>Fifty-two slow-growing strains were isolated from root nodules of Calicotome spinosa grown in the Northeast of Algeria and grouped in 24 rep-PCR clusters. One representative strain for each profile was further phylogenetically characterized. The nearly complete 16S rRNA gene sequence indicated that all strains were affiliated to Bradyrhizobium. Multi-Locus Sequence Analysis (MLSA) of the atpD, glnII and recA genes and of the 16S-23S rRNA internal transcribed spacer (ITS) showed that these strains formed four divergent clusters: one close to Bradyrhizobium canariense and Bradyrhizobium lupini and three others separate from all the described species, representing three putative new Bradyrhizobium species. A phylogenetic analysis based on the nodC gene sequence affiliated the strains to either of the two symbiovars, genistearum or retamae.</description><subject>Biotechnology</subject><subject>Bradyrhizobium</subject><subject>Calicotome spinosa</subject><subject>Life Sciences</subject><subject>Microbiology and Parasitology</subject><subject>MLSA</subject><subject>Root nodules</subject><subject>Symbiosis</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kU-P0zAQxS0EYsvCJ0BCPsIhYcZx_h04lApYpAo4wNly7UnrKomL7VQKn550u-yR02hGv3nzNI-x1wg5Albvj3mc9WnIBWCTQ5kDlE_YCitsMmgb-ZStoBZFJkDADXsR4xEAZVvhc3Yj2qZFBLlixx-Hufd7Gik5w607U4guzdx3_GPQdg4H98fv3DTwmIJ2Y-Sjt1Ovkxv3fKN7Z3zyA_F4cqOPmruRpwPxbz4sRcd0EVr3ewpOv2TPOt1HevVQb9mvz59-bu6y7fcvXzfrbWaKtkgZ7kg2oBtrFr-6tkjCUiEQTFeIrqyl7Za-3kkJ9YJWVBXQCDDWFh0UUBa37N1V96B7dQpu0GFWXjt1t96qywywLStEPOPCvr2yp-B_TxSTGlw01Pd6JD9FJaCUrZCykgtaXFETfIyBukdtBHUJRB3VfSDqEoiCUsG9mTcPB6bdQPZx518CC_DhCtDykrOjoKJxNBqyLpBJynr33wN_AUCAnmo</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Salmi, Adouda</creator><creator>Boulila, Farida</creator><creator>Bourebaba, Yasmina</creator><creator>Le Roux, Christine</creator><creator>Belhadi, Djellali</creator><creator>de Lajudie, Philippe</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-2196-6352</orcidid><orcidid>https://orcid.org/0000-0003-0668-3589</orcidid></search><sort><creationdate>20180901</creationdate><title>Phylogenetic diversity of Bradyrhizobium strains nodulating Calicotome spinosa in the Northeast of Algeria</title><author>Salmi, Adouda ; Boulila, Farida ; Bourebaba, Yasmina ; Le Roux, Christine ; Belhadi, Djellali ; de Lajudie, Philippe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-1be480a8dc001a7d1e2de3210cf32f574dfde37b4407be46e630820cdd3f03053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biotechnology</topic><topic>Bradyrhizobium</topic><topic>Calicotome spinosa</topic><topic>Life Sciences</topic><topic>Microbiology and Parasitology</topic><topic>MLSA</topic><topic>Root nodules</topic><topic>Symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salmi, Adouda</creatorcontrib><creatorcontrib>Boulila, Farida</creatorcontrib><creatorcontrib>Bourebaba, Yasmina</creatorcontrib><creatorcontrib>Le Roux, Christine</creatorcontrib><creatorcontrib>Belhadi, Djellali</creatorcontrib><creatorcontrib>de Lajudie, Philippe</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salmi, Adouda</au><au>Boulila, Farida</au><au>Bourebaba, Yasmina</au><au>Le Roux, Christine</au><au>Belhadi, Djellali</au><au>de Lajudie, Philippe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic diversity of Bradyrhizobium strains nodulating Calicotome spinosa in the Northeast of Algeria</atitle><jtitle>Systematic and applied microbiology</jtitle><addtitle>Syst Appl Microbiol</addtitle><date>2018-09-01</date><risdate>2018</risdate><volume>41</volume><issue>5</issue><spage>452</spage><epage>459</epage><pages>452-459</pages><issn>0723-2020</issn><eissn>1618-0984</eissn><abstract>Fifty-two slow-growing strains were isolated from root nodules of Calicotome spinosa grown in the Northeast of Algeria and grouped in 24 rep-PCR clusters. One representative strain for each profile was further phylogenetically characterized. The nearly complete 16S rRNA gene sequence indicated that all strains were affiliated to Bradyrhizobium. Multi-Locus Sequence Analysis (MLSA) of the atpD, glnII and recA genes and of the 16S-23S rRNA internal transcribed spacer (ITS) showed that these strains formed four divergent clusters: one close to Bradyrhizobium canariense and Bradyrhizobium lupini and three others separate from all the described species, representing three putative new Bradyrhizobium species. A phylogenetic analysis based on the nodC gene sequence affiliated the strains to either of the two symbiovars, genistearum or retamae.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>29891104</pmid><doi>10.1016/j.syapm.2018.05.005</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-2196-6352</orcidid><orcidid>https://orcid.org/0000-0003-0668-3589</orcidid></addata></record> |
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| ispartof | Systematic and applied microbiology, 2018-09, Vol.41 (5), p.452-459 |
| issn | 0723-2020 1618-0984 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Biotechnology Bradyrhizobium Calicotome spinosa Life Sciences Microbiology and Parasitology MLSA Root nodules Symbiosis |
| title | Phylogenetic diversity of Bradyrhizobium strains nodulating Calicotome spinosa in the Northeast of Algeria |
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