Pseudomonas fluorescens alters the intestinal barrier function by modulating IL-1β expression through hematopoietic NOD2 signaling
Ileal Crohn's disease is related to NOD2 mutations and to a gut barrier dysfunction. Pseudomonas fluorescens has also been associated with ileal Crohn's disease. The aim of this study was to determine the impact of P. fluorescens on the paracellular permeability in ileum and Peyer's p...
Gespeichert in:
| Veröffentlicht in: | Inflammatory bowel diseases 2015-03, Vol.21 (3), p.543-555 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 555 |
|---|---|
| container_issue | 3 |
| container_start_page | 543 |
| container_title | Inflammatory bowel diseases |
| container_volume | 21 |
| creator | Alnabhani, Ziad Montcuquet, Nicolas Biaggini, Kelly Dussaillant, Monique Roy, Maryline Ogier-Denis, Eric Madi, Amar Jallane, Abdelhak Feuilloley, Marc Hugot, Jean-Pierre Connil, Nathalie Barreau, Fréderick |
| description | Ileal Crohn's disease is related to NOD2 mutations and to a gut barrier dysfunction. Pseudomonas fluorescens has also been associated with ileal Crohn's disease. The aim of this study was to determine the impact of P. fluorescens on the paracellular permeability in ileum and Peyer's patches.
To explore this question, in vivo and ex vivo experiments were performed in wild-type, Nod2, Nod2, and IL-1R mice together with in vitro analyses using the Caco-2 (epithelial) and the THP-1 (monocyte) human cell lines.
Pseudomonas fluorescens increased the paracellular permeability of the intestinal mucosa through the secretion of IL-1β by the immune cell populations and the activation of myosin light chain kinase in the epithelial cells. Induction of the IL-1β pathway required the expression of Nod2 in the hematopoietic compartment, and muramyl dipeptide (a Nod2 ligand) had an inhibitory effect.
Pseudomonas fluorescens thus alters the homeostasis of the epithelial barrier function by a mechanism similar to that previously observed for Yersinia pseudotuberculosis. This work further documents a putative role of psychrotrophic bacteria in Crohn's disease. |
| doi_str_mv | 10.1097/MIB.0000000000000291 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_01938122v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1655520613</sourcerecordid><originalsourceid>FETCH-LOGICAL-c368t-4d8aafb2ffdad8b028f3ca9b59c7995a902dbd033f36de44050d403047615b343</originalsourceid><addsrcrecordid>eNpdkc9OHSEUxkmjqdb2DRrDsl2M8ncGltbaepOrdqFrwgxwBzMz3AJjdO0b-SA-k9xcaxphATnn933k8AHwFaMjjGRzfLH4cYT-X0TiD2Afc1pXTDC2U-6oERWSUuyBTyndFqRs-RHsEV5ziUSzDx7_JDubMIZJJ-iGOUSbOjslqIdsY4K5t9BP2absJz3AVsfobYRunrrswwTbBzgGMw-69Fdwsazw8xO09-tikzb93Mcwr3rY21HnsA7eZt_By6ufBCa_KpZF9hnsOj0k--X1PAA3v86uT8-r5dXvxenJsupoLXLFjNDatcQ5o41oERGOdlq2XHaNlFxLRExrEKWO1sYyhjgyDFHEmhrzljJ6AL5vfXs9qHX0o44PKmivzk-WalNDWFKBCbnDhf22Zdcx_J3L-Gr05WOGQU82zEnhmnNOUI1pQdkW7WJIKVr35o2R2kSlSlTqfVRFdvj6wtyO1ryJ_mVDXwDXB5E8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1655520613</pqid></control><display><type>article</type><title>Pseudomonas fluorescens alters the intestinal barrier function by modulating IL-1β expression through hematopoietic NOD2 signaling</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Alnabhani, Ziad ; Montcuquet, Nicolas ; Biaggini, Kelly ; Dussaillant, Monique ; Roy, Maryline ; Ogier-Denis, Eric ; Madi, Amar ; Jallane, Abdelhak ; Feuilloley, Marc ; Hugot, Jean-Pierre ; Connil, Nathalie ; Barreau, Fréderick</creator><creatorcontrib>Alnabhani, Ziad ; Montcuquet, Nicolas ; Biaggini, Kelly ; Dussaillant, Monique ; Roy, Maryline ; Ogier-Denis, Eric ; Madi, Amar ; Jallane, Abdelhak ; Feuilloley, Marc ; Hugot, Jean-Pierre ; Connil, Nathalie ; Barreau, Fréderick</creatorcontrib><description>Ileal Crohn's disease is related to NOD2 mutations and to a gut barrier dysfunction. Pseudomonas fluorescens has also been associated with ileal Crohn's disease. The aim of this study was to determine the impact of P. fluorescens on the paracellular permeability in ileum and Peyer's patches.
To explore this question, in vivo and ex vivo experiments were performed in wild-type, Nod2, Nod2, and IL-1R mice together with in vitro analyses using the Caco-2 (epithelial) and the THP-1 (monocyte) human cell lines.
Pseudomonas fluorescens increased the paracellular permeability of the intestinal mucosa through the secretion of IL-1β by the immune cell populations and the activation of myosin light chain kinase in the epithelial cells. Induction of the IL-1β pathway required the expression of Nod2 in the hematopoietic compartment, and muramyl dipeptide (a Nod2 ligand) had an inhibitory effect.
Pseudomonas fluorescens thus alters the homeostasis of the epithelial barrier function by a mechanism similar to that previously observed for Yersinia pseudotuberculosis. This work further documents a putative role of psychrotrophic bacteria in Crohn's disease.</description><identifier>ISSN: 1078-0998</identifier><identifier>EISSN: 1536-4844</identifier><identifier>DOI: 10.1097/MIB.0000000000000291</identifier><identifier>PMID: 25659087</identifier><language>eng</language><publisher>England: Lippincott, Williams & Wilkins</publisher><subject>Animals ; Bacteriology ; Biochemistry, Molecular Biology ; Blotting, Western ; Caco-2 Cells ; Cell Behavior ; Cell Membrane Permeability ; Cells, Cultured ; Cellular Biology ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - metabolism ; Hematopoietic Stem Cells - microbiology ; Humans ; Interleukin-1beta - genetics ; Interleukin-1beta - metabolism ; Intestinal Mucosa - cytology ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - microbiology ; Life Sciences ; Macrophages - cytology ; Macrophages - metabolism ; Macrophages - microbiology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Microbiology and Parasitology ; Molecular biology ; Nod2 Signaling Adaptor Protein - genetics ; Nod2 Signaling Adaptor Protein - metabolism ; Nod2 Signaling Adaptor Protein - physiology ; Pseudomonas fluorescens - physiology ; Real-Time Polymerase Chain Reaction ; Receptors, Interleukin-1 - physiology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Signal Transduction</subject><ispartof>Inflammatory bowel diseases, 2015-03, Vol.21 (3), p.543-555</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-4d8aafb2ffdad8b028f3ca9b59c7995a902dbd033f36de44050d403047615b343</citedby><cites>FETCH-LOGICAL-c368t-4d8aafb2ffdad8b028f3ca9b59c7995a902dbd033f36de44050d403047615b343</cites><orcidid>0000-0001-6467-4336</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25659087$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://normandie-univ.hal.science/hal-01938122$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Alnabhani, Ziad</creatorcontrib><creatorcontrib>Montcuquet, Nicolas</creatorcontrib><creatorcontrib>Biaggini, Kelly</creatorcontrib><creatorcontrib>Dussaillant, Monique</creatorcontrib><creatorcontrib>Roy, Maryline</creatorcontrib><creatorcontrib>Ogier-Denis, Eric</creatorcontrib><creatorcontrib>Madi, Amar</creatorcontrib><creatorcontrib>Jallane, Abdelhak</creatorcontrib><creatorcontrib>Feuilloley, Marc</creatorcontrib><creatorcontrib>Hugot, Jean-Pierre</creatorcontrib><creatorcontrib>Connil, Nathalie</creatorcontrib><creatorcontrib>Barreau, Fréderick</creatorcontrib><title>Pseudomonas fluorescens alters the intestinal barrier function by modulating IL-1β expression through hematopoietic NOD2 signaling</title><title>Inflammatory bowel diseases</title><addtitle>Inflamm Bowel Dis</addtitle><description>Ileal Crohn's disease is related to NOD2 mutations and to a gut barrier dysfunction. Pseudomonas fluorescens has also been associated with ileal Crohn's disease. The aim of this study was to determine the impact of P. fluorescens on the paracellular permeability in ileum and Peyer's patches.
To explore this question, in vivo and ex vivo experiments were performed in wild-type, Nod2, Nod2, and IL-1R mice together with in vitro analyses using the Caco-2 (epithelial) and the THP-1 (monocyte) human cell lines.
Pseudomonas fluorescens increased the paracellular permeability of the intestinal mucosa through the secretion of IL-1β by the immune cell populations and the activation of myosin light chain kinase in the epithelial cells. Induction of the IL-1β pathway required the expression of Nod2 in the hematopoietic compartment, and muramyl dipeptide (a Nod2 ligand) had an inhibitory effect.
Pseudomonas fluorescens thus alters the homeostasis of the epithelial barrier function by a mechanism similar to that previously observed for Yersinia pseudotuberculosis. This work further documents a putative role of psychrotrophic bacteria in Crohn's disease.</description><subject>Animals</subject><subject>Bacteriology</subject><subject>Biochemistry, Molecular Biology</subject><subject>Blotting, Western</subject><subject>Caco-2 Cells</subject><subject>Cell Behavior</subject><subject>Cell Membrane Permeability</subject><subject>Cells, Cultured</subject><subject>Cellular Biology</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Hematopoietic Stem Cells - microbiology</subject><subject>Humans</subject><subject>Interleukin-1beta - genetics</subject><subject>Interleukin-1beta - metabolism</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - microbiology</subject><subject>Life Sciences</subject><subject>Macrophages - cytology</subject><subject>Macrophages - metabolism</subject><subject>Macrophages - microbiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Microbiology and Parasitology</subject><subject>Molecular biology</subject><subject>Nod2 Signaling Adaptor Protein - genetics</subject><subject>Nod2 Signaling Adaptor Protein - metabolism</subject><subject>Nod2 Signaling Adaptor Protein - physiology</subject><subject>Pseudomonas fluorescens - physiology</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptors, Interleukin-1 - physiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Signal Transduction</subject><issn>1078-0998</issn><issn>1536-4844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc9OHSEUxkmjqdb2DRrDsl2M8ncGltbaepOrdqFrwgxwBzMz3AJjdO0b-SA-k9xcaxphATnn933k8AHwFaMjjGRzfLH4cYT-X0TiD2Afc1pXTDC2U-6oERWSUuyBTyndFqRs-RHsEV5ziUSzDx7_JDubMIZJJ-iGOUSbOjslqIdsY4K5t9BP2absJz3AVsfobYRunrrswwTbBzgGMw-69Fdwsazw8xO09-tikzb93Mcwr3rY21HnsA7eZt_By6ufBCa_KpZF9hnsOj0k--X1PAA3v86uT8-r5dXvxenJsupoLXLFjNDatcQ5o41oERGOdlq2XHaNlFxLRExrEKWO1sYyhjgyDFHEmhrzljJ6AL5vfXs9qHX0o44PKmivzk-WalNDWFKBCbnDhf22Zdcx_J3L-Gr05WOGQU82zEnhmnNOUI1pQdkW7WJIKVr35o2R2kSlSlTqfVRFdvj6wtyO1ryJ_mVDXwDXB5E8</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Alnabhani, Ziad</creator><creator>Montcuquet, Nicolas</creator><creator>Biaggini, Kelly</creator><creator>Dussaillant, Monique</creator><creator>Roy, Maryline</creator><creator>Ogier-Denis, Eric</creator><creator>Madi, Amar</creator><creator>Jallane, Abdelhak</creator><creator>Feuilloley, Marc</creator><creator>Hugot, Jean-Pierre</creator><creator>Connil, Nathalie</creator><creator>Barreau, Fréderick</creator><general>Lippincott, Williams & Wilkins</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-6467-4336</orcidid></search><sort><creationdate>20150301</creationdate><title>Pseudomonas fluorescens alters the intestinal barrier function by modulating IL-1β expression through hematopoietic NOD2 signaling</title><author>Alnabhani, Ziad ; Montcuquet, Nicolas ; Biaggini, Kelly ; Dussaillant, Monique ; Roy, Maryline ; Ogier-Denis, Eric ; Madi, Amar ; Jallane, Abdelhak ; Feuilloley, Marc ; Hugot, Jean-Pierre ; Connil, Nathalie ; Barreau, Fréderick</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-4d8aafb2ffdad8b028f3ca9b59c7995a902dbd033f36de44050d403047615b343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Bacteriology</topic><topic>Biochemistry, Molecular Biology</topic><topic>Blotting, Western</topic><topic>Caco-2 Cells</topic><topic>Cell Behavior</topic><topic>Cell Membrane Permeability</topic><topic>Cells, Cultured</topic><topic>Cellular Biology</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Hematopoietic Stem Cells - microbiology</topic><topic>Humans</topic><topic>Interleukin-1beta - genetics</topic><topic>Interleukin-1beta - metabolism</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - microbiology</topic><topic>Life Sciences</topic><topic>Macrophages - cytology</topic><topic>Macrophages - metabolism</topic><topic>Macrophages - microbiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Microbiology and Parasitology</topic><topic>Molecular biology</topic><topic>Nod2 Signaling Adaptor Protein - genetics</topic><topic>Nod2 Signaling Adaptor Protein - metabolism</topic><topic>Nod2 Signaling Adaptor Protein - physiology</topic><topic>Pseudomonas fluorescens - physiology</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptors, Interleukin-1 - physiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alnabhani, Ziad</creatorcontrib><creatorcontrib>Montcuquet, Nicolas</creatorcontrib><creatorcontrib>Biaggini, Kelly</creatorcontrib><creatorcontrib>Dussaillant, Monique</creatorcontrib><creatorcontrib>Roy, Maryline</creatorcontrib><creatorcontrib>Ogier-Denis, Eric</creatorcontrib><creatorcontrib>Madi, Amar</creatorcontrib><creatorcontrib>Jallane, Abdelhak</creatorcontrib><creatorcontrib>Feuilloley, Marc</creatorcontrib><creatorcontrib>Hugot, Jean-Pierre</creatorcontrib><creatorcontrib>Connil, Nathalie</creatorcontrib><creatorcontrib>Barreau, Fréderick</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Inflammatory bowel diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alnabhani, Ziad</au><au>Montcuquet, Nicolas</au><au>Biaggini, Kelly</au><au>Dussaillant, Monique</au><au>Roy, Maryline</au><au>Ogier-Denis, Eric</au><au>Madi, Amar</au><au>Jallane, Abdelhak</au><au>Feuilloley, Marc</au><au>Hugot, Jean-Pierre</au><au>Connil, Nathalie</au><au>Barreau, Fréderick</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pseudomonas fluorescens alters the intestinal barrier function by modulating IL-1β expression through hematopoietic NOD2 signaling</atitle><jtitle>Inflammatory bowel diseases</jtitle><addtitle>Inflamm Bowel Dis</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>21</volume><issue>3</issue><spage>543</spage><epage>555</epage><pages>543-555</pages><issn>1078-0998</issn><eissn>1536-4844</eissn><abstract>Ileal Crohn's disease is related to NOD2 mutations and to a gut barrier dysfunction. Pseudomonas fluorescens has also been associated with ileal Crohn's disease. The aim of this study was to determine the impact of P. fluorescens on the paracellular permeability in ileum and Peyer's patches.
To explore this question, in vivo and ex vivo experiments were performed in wild-type, Nod2, Nod2, and IL-1R mice together with in vitro analyses using the Caco-2 (epithelial) and the THP-1 (monocyte) human cell lines.
Pseudomonas fluorescens increased the paracellular permeability of the intestinal mucosa through the secretion of IL-1β by the immune cell populations and the activation of myosin light chain kinase in the epithelial cells. Induction of the IL-1β pathway required the expression of Nod2 in the hematopoietic compartment, and muramyl dipeptide (a Nod2 ligand) had an inhibitory effect.
Pseudomonas fluorescens thus alters the homeostasis of the epithelial barrier function by a mechanism similar to that previously observed for Yersinia pseudotuberculosis. This work further documents a putative role of psychrotrophic bacteria in Crohn's disease.</abstract><cop>England</cop><pub>Lippincott, Williams & Wilkins</pub><pmid>25659087</pmid><doi>10.1097/MIB.0000000000000291</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-6467-4336</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1078-0998 |
| ispartof | Inflammatory bowel diseases, 2015-03, Vol.21 (3), p.543-555 |
| issn | 1078-0998 1536-4844 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_01938122v1 |
| source | MEDLINE; Journals@Ovid Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Bacteriology Biochemistry, Molecular Biology Blotting, Western Caco-2 Cells Cell Behavior Cell Membrane Permeability Cells, Cultured Cellular Biology Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - metabolism Hematopoietic Stem Cells - microbiology Humans Interleukin-1beta - genetics Interleukin-1beta - metabolism Intestinal Mucosa - cytology Intestinal Mucosa - metabolism Intestinal Mucosa - microbiology Life Sciences Macrophages - cytology Macrophages - metabolism Macrophages - microbiology Mice Mice, Inbred C57BL Mice, Knockout Microbiology and Parasitology Molecular biology Nod2 Signaling Adaptor Protein - genetics Nod2 Signaling Adaptor Protein - metabolism Nod2 Signaling Adaptor Protein - physiology Pseudomonas fluorescens - physiology Real-Time Polymerase Chain Reaction Receptors, Interleukin-1 - physiology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Signal Transduction |
| title | Pseudomonas fluorescens alters the intestinal barrier function by modulating IL-1β expression through hematopoietic NOD2 signaling |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T20%3A38%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pseudomonas%20fluorescens%20alters%20the%20intestinal%20barrier%20function%20by%20modulating%20IL-1%CE%B2%20expression%20through%20hematopoietic%20NOD2%20signaling&rft.jtitle=Inflammatory%20bowel%20diseases&rft.au=Alnabhani,%20Ziad&rft.date=2015-03-01&rft.volume=21&rft.issue=3&rft.spage=543&rft.epage=555&rft.pages=543-555&rft.issn=1078-0998&rft.eissn=1536-4844&rft_id=info:doi/10.1097/MIB.0000000000000291&rft_dat=%3Cproquest_hal_p%3E1655520613%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1655520613&rft_id=info:pmid/25659087&rfr_iscdi=true |