Extraction and fractionation of polysaccharides and B-phycoerythrin from the microalga Porphyridium cruentum by membrane technology
Porphyridium species are exploited for the production of exopolysaccharides and B-phycoerythrin, two high-value compounds. Extraction and purification of these two substances often involve antagonistic processes (precipitation with ethanol, isoelectric precipitation, aqueous two-phase systems, chrom...
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| Veröffentlicht in: | Algal research (Amsterdam) 2014-07, Vol.5, p.258-263 |
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| container_title | Algal research (Amsterdam) |
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| creator | Marcati, Alain Ursu, Alina Violeta Laroche, Céline Soanen, Nastasia Marchal, Luc Jubeau, Sebastien Djelveh, Gholamreza Michaud, Philippe |
| description | Porphyridium species are exploited for the production of exopolysaccharides and B-phycoerythrin, two high-value compounds. Extraction and purification of these two substances often involve antagonistic processes (precipitation with ethanol, isoelectric precipitation, aqueous two-phase systems, chromatography and ultrafiltration) that work better for one than for the other. We developed a two-step membrane process using polyethersulfone flat membranes with a filtration surface of 0.1m2 to recover both B-phycoerythrin and soluble polysaccharides from Porphyridium biomass with high polysaccharide content. A first ultrafiltration at 300,000 yielded high molecular weight polysaccharides. The resulting 300,000 permeate was then run through a second, 10,000 membrane to recover BPE. This procedure enabled us to extract 48% of B-phycoerythrin free of polysaccharide with a purity index of 2.3. |
| doi_str_mv | 10.1016/j.algal.2014.03.006 |
| format | Article |
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| ispartof | Algal research (Amsterdam), 2014-07, Vol.5, p.258-263 |
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| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | B-phycoerythrin Chemical and Process Engineering Engineering Sciences Microalgae Polysaccharide Porphyridium |
| title | Extraction and fractionation of polysaccharides and B-phycoerythrin from the microalga Porphyridium cruentum by membrane technology |
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