Self-interference 3D super-resolution microscopy for deep tissue investigations
Fluorescence localization microscopy has achieved near-molecular resolution capable of revealing ultra-structures, with a broad range of applications, especially in cellular biology. However, it remains challenging to attain such resolution in three dimensions and inside biological tissues beyond th...
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| Veröffentlicht in: | Nature methods 2018-06, Vol.15 (6), p.449-454 |
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| creator | Bon, Pierre Linarès-Loyez, Jeanne Feyeux, Maxime Alessandri, Kevin Lounis, Brahim Nassoy, Pierre Cognet, Laurent |
| description | Fluorescence localization microscopy has achieved near-molecular resolution capable of revealing ultra-structures, with a broad range of applications, especially in cellular biology. However, it remains challenging to attain such resolution in three dimensions and inside biological tissues beyond the first cell layer. Here we introduce SELFI, a framework for 3D single-molecule localization within multicellular specimens and tissues. The approach relies on self-interference generated within the microscope's point spread function (PSF) to simultaneously encode equiphase and intensity fluorescence signals, which together provide the 3D position of an emitter. We combined SELFI with conventional localization microscopy to visualize F-actin 3D filament networks and reveal the spatial distribution of the transcription factor OCT4 in human induced pluripotent stem cells at depths up to 50 µm inside uncleared tissue spheroids. SELFI paves the way to nanoscale investigations of native cellular processes in intact tissues.
Several methods make it possible to obtain depth information in 3D super-resolution microscopy. Here the authors exploit a self-interference phenomenon to allow deep imaging within tissue samples, as well as isotropic imaging. |
| doi_str_mv | 10.1038/s41592-018-0005-3 |
| format | Article |
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Several methods make it possible to obtain depth information in 3D super-resolution microscopy. Here the authors exploit a self-interference phenomenon to allow deep imaging within tissue samples, as well as isotropic imaging.</description><identifier>ISSN: 1548-7091</identifier><identifier>EISSN: 1548-7105</identifier><identifier>DOI: 10.1038/s41592-018-0005-3</identifier><identifier>PMID: 29713082</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>631/1647/328/1650 ; 631/1647/328/2238 ; Actin ; Bioinformatics ; Biological Microscopy ; Biological Physics ; Biological specimens ; Biological Techniques ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Cellular structure ; Emitters ; Fluorescence ; Fluorescence microscopy ; Interference ; Life Sciences ; Localization ; Methods ; Microscopy ; Molecular chains ; Muscle proteins ; Observations ; Oct-4 protein ; Physics ; Pluripotency ; Point spread functions ; Position (location) ; Proteomics ; Resolution (Optics) ; Spatial distribution ; Spheroids ; Stem cells ; Three-dimensional imaging ; Tissues ; Tissues (Anatomy)</subject><ispartof>Nature methods, 2018-06, Vol.15 (6), p.449-454</ispartof><rights>The Author(s) 2018</rights><rights>COPYRIGHT 2018 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jun 2018</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-d72a8af4427201e297ace3547353940fdcc913a83e5f65ab5d885296f19776413</citedby><cites>FETCH-LOGICAL-c473t-d72a8af4427201e297ace3547353940fdcc913a83e5f65ab5d885296f19776413</cites><orcidid>0000-0002-3573-5387 ; 0000-0002-7820-5327 ; 0000-0002-1986-3493 ; 0000-0003-2216-2839</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/s41592-018-0005-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/s41592-018-0005-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29713082$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01914540$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Bon, Pierre</creatorcontrib><creatorcontrib>Linarès-Loyez, Jeanne</creatorcontrib><creatorcontrib>Feyeux, Maxime</creatorcontrib><creatorcontrib>Alessandri, Kevin</creatorcontrib><creatorcontrib>Lounis, Brahim</creatorcontrib><creatorcontrib>Nassoy, Pierre</creatorcontrib><creatorcontrib>Cognet, Laurent</creatorcontrib><title>Self-interference 3D super-resolution microscopy for deep tissue investigations</title><title>Nature methods</title><addtitle>Nat Methods</addtitle><addtitle>Nat Methods</addtitle><description>Fluorescence localization microscopy has achieved near-molecular resolution capable of revealing ultra-structures, with a broad range of applications, especially in cellular biology. However, it remains challenging to attain such resolution in three dimensions and inside biological tissues beyond the first cell layer. Here we introduce SELFI, a framework for 3D single-molecule localization within multicellular specimens and tissues. The approach relies on self-interference generated within the microscope's point spread function (PSF) to simultaneously encode equiphase and intensity fluorescence signals, which together provide the 3D position of an emitter. We combined SELFI with conventional localization microscopy to visualize F-actin 3D filament networks and reveal the spatial distribution of the transcription factor OCT4 in human induced pluripotent stem cells at depths up to 50 µm inside uncleared tissue spheroids. SELFI paves the way to nanoscale investigations of native cellular processes in intact tissues.
Several methods make it possible to obtain depth information in 3D super-resolution microscopy. Here the authors exploit a self-interference phenomenon to allow deep imaging within tissue samples, as well as isotropic imaging.</description><subject>631/1647/328/1650</subject><subject>631/1647/328/2238</subject><subject>Actin</subject><subject>Bioinformatics</subject><subject>Biological Microscopy</subject><subject>Biological Physics</subject><subject>Biological specimens</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Cellular structure</subject><subject>Emitters</subject><subject>Fluorescence</subject><subject>Fluorescence microscopy</subject><subject>Interference</subject><subject>Life Sciences</subject><subject>Localization</subject><subject>Methods</subject><subject>Microscopy</subject><subject>Molecular chains</subject><subject>Muscle proteins</subject><subject>Observations</subject><subject>Oct-4 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methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bon, Pierre</au><au>Linarès-Loyez, Jeanne</au><au>Feyeux, Maxime</au><au>Alessandri, Kevin</au><au>Lounis, Brahim</au><au>Nassoy, Pierre</au><au>Cognet, Laurent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Self-interference 3D super-resolution microscopy for deep tissue investigations</atitle><jtitle>Nature methods</jtitle><stitle>Nat Methods</stitle><addtitle>Nat Methods</addtitle><date>2018-06-01</date><risdate>2018</risdate><volume>15</volume><issue>6</issue><spage>449</spage><epage>454</epage><pages>449-454</pages><issn>1548-7091</issn><eissn>1548-7105</eissn><abstract>Fluorescence localization microscopy has achieved near-molecular resolution capable of revealing ultra-structures, with a broad range of applications, especially in cellular biology. However, it remains challenging to attain such resolution in three dimensions and inside biological tissues beyond the first cell layer. Here we introduce SELFI, a framework for 3D single-molecule localization within multicellular specimens and tissues. The approach relies on self-interference generated within the microscope's point spread function (PSF) to simultaneously encode equiphase and intensity fluorescence signals, which together provide the 3D position of an emitter. We combined SELFI with conventional localization microscopy to visualize F-actin 3D filament networks and reveal the spatial distribution of the transcription factor OCT4 in human induced pluripotent stem cells at depths up to 50 µm inside uncleared tissue spheroids. SELFI paves the way to nanoscale investigations of native cellular processes in intact tissues.
Several methods make it possible to obtain depth information in 3D super-resolution microscopy. Here the authors exploit a self-interference phenomenon to allow deep imaging within tissue samples, as well as isotropic imaging.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>29713082</pmid><doi>10.1038/s41592-018-0005-3</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-3573-5387</orcidid><orcidid>https://orcid.org/0000-0002-7820-5327</orcidid><orcidid>https://orcid.org/0000-0002-1986-3493</orcidid><orcidid>https://orcid.org/0000-0003-2216-2839</orcidid></addata></record> |
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| ispartof | Nature methods, 2018-06, Vol.15 (6), p.449-454 |
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| subjects | 631/1647/328/1650 631/1647/328/2238 Actin Bioinformatics Biological Microscopy Biological Physics Biological specimens Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Cellular structure Emitters Fluorescence Fluorescence microscopy Interference Life Sciences Localization Methods Microscopy Molecular chains Muscle proteins Observations Oct-4 protein Physics Pluripotency Point spread functions Position (location) Proteomics Resolution (Optics) Spatial distribution Spheroids Stem cells Three-dimensional imaging Tissues Tissues (Anatomy) |
| title | Self-interference 3D super-resolution microscopy for deep tissue investigations |
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