Staphylococcus aureus infective endocarditis versus bacteremia strains: Subtle genetic differences at stake
Infective endocarditis (IE)(1) is a severe condition complicating 10–25% of Staphylococcus aureus bacteremia. Although host-related IE risk factors have been identified, the involvement of bacterial features in IE complication is still unclear. We characterized strictly defined IE and bacteremia iso...
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| Veröffentlicht in: | Infection, genetics and evolution genetics and evolution, 2015-12, Vol.36, p.524-530 |
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| creator | Bouchiat, Coralie Moreau, Karen Devillard, Sébastien Rasigade, Jean-Philippe Mosnier, Amandine Geissmann, Tom Bes, Michèle Tristan, Anne Lina, Gérard Laurent, Frédéric Piroth, Lionel Aissa, Nejla Duval, Xavier Le Moing, Vincent Vandenesch, François Chirouze, Catherine Curlier, Elodie Descottes-Genon, Cécile Hoen, Bruno Patry, Isabelle Vettoretti, Lucie Chavanet, Pascal Eicher, Jean-Christophe Gohier-Treuvelot, Sandrine Greusard, Marie-Christine Neuwirth, Catherine Péchinot, André Piroth, Lionel Célard, Marie Cornu, Catherine Delahaye, François Hadid, Malika Rausch, Pascale Coma, Audrey Galtier, Florence Géraud, Philippe Jean-Pierre, Hélène Le Moing, Vincent Sportouch, Catherine Reynes, Jacques Aissa, Nejla Doco-Lecompte, Thanh Goehringer, François Keil, Nathalie Letranchant, Lorraine Malela, Hepher May, Thierry Selton-Suty, Christine Bedos, Nathalie Lavigne, Jean-Philippe Lechiche, Catherine Sotto, Albert Duval, Xavier Habensus, Emila Ilic Iung, Bernard Leport, Catherine Longuet, Pascale Ruimy, Raymond Bellissant, Eric Donnio, Pierre-Yves Le Gac, Fabienne Michelet, Christian Revest, Matthieu Tattevin, Pierre Thebault, Elise Alla, François Braquet, Pierre Erpelding, Marie-Line Minary, Laetitia Tubiana, Sarah Bès, Michèle Etienne, Jérôme Tristan, Anne Vandenesch, François Barbas, Sandrine Delonca, Christine Sussmuth, Virginie Verchère, Anne |
| description | Infective endocarditis (IE)(1) is a severe condition complicating 10–25% of Staphylococcus aureus bacteremia. Although host-related IE risk factors have been identified, the involvement of bacterial features in IE complication is still unclear. We characterized strictly defined IE and bacteremia isolates and searched for discriminant features. S. aureus isolates causing community-acquired, definite native-valve IE (n=72) and bacteremia (n=54) were collected prospectively as part of a French multicenter cohort. Phenotypic traits previously reported or hypothesized to be involved in staphylococcal IE pathogenesis were tested. In parallel, the genotypic profiles of all isolates, obtained by microarray, were analyzed by discriminant analysis of principal components (DAPC)(2). No significant difference was observed between IE and bacteremia strains, regarding either phenotypic or genotypic univariate analyses. However, the multivariate statistical tool DAPC, applied on microarray data, segregated IE and bacteremia isolates: IE isolates were correctly reassigned as such in 80.6% of the cases (C-statistic 0.83, P |
| doi_str_mv | 10.1016/j.meegid.2015.08.029 |
| format | Article |
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Although host-related IE risk factors have been identified, the involvement of bacterial features in IE complication is still unclear. We characterized strictly defined IE and bacteremia isolates and searched for discriminant features. S. aureus isolates causing community-acquired, definite native-valve IE (n=72) and bacteremia (n=54) were collected prospectively as part of a French multicenter cohort. Phenotypic traits previously reported or hypothesized to be involved in staphylococcal IE pathogenesis were tested. In parallel, the genotypic profiles of all isolates, obtained by microarray, were analyzed by discriminant analysis of principal components (DAPC)(2). No significant difference was observed between IE and bacteremia strains, regarding either phenotypic or genotypic univariate analyses. However, the multivariate statistical tool DAPC, applied on microarray data, segregated IE and bacteremia isolates: IE isolates were correctly reassigned as such in 80.6% of the cases (C-statistic 0.83, P<0.001). The performance of this model was confirmed with an independent French collection IE and bacteremia isolates (78.8% reassignment, C-statistic 0.65, P<0.01). Finally, a simple linear discriminant function based on a subset of 8 genetic markers retained valuable performance both in study collection (86.1%, P<0.001) and in the independent validation collection (81.8%, P<0.01). We here show that community-acquired IE and bacteremia S. aureus isolates are genetically distinct based on subtle combinations of genetic markers. This finding provides the proof of concept that bacterial characteristics may contribute to the occurrence of IE in patients with S. aureus bacteremia.
•Infective endocarditis (IE) and bacteremia (Ba) S. aureus isolates are collected.•We search for any features discriminating IE and Ba isolates.•No single phenotypic or genotypic trait discriminates the two S. aureus groups.•Multivariate analysis shows subtle genetic differences between IE and Ba isolates.•Results are confirmed on an independent collection of S. aureus isolates.</description><identifier>ISSN: 1567-1348</identifier><identifier>EISSN: 1567-7257</identifier><identifier>DOI: 10.1016/j.meegid.2015.08.029</identifier><identifier>PMID: 26318542</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Bacteremia ; Bacteremia - microbiology ; Community-Acquired Infections ; DAPC ; Endocarditis, Bacterial - microbiology ; Genes, Bacterial ; Genetic distinction ; Genetic Markers ; Genetic Variation ; Genotype ; Human health and pathology ; Humans ; Infectious diseases ; Infective endocarditis ; Life Sciences ; Phenotype ; Reproducibility of Results ; Staphylococcal Infections - microbiology ; Staphylococcus aureus ; Staphylococcus aureus - classification ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification</subject><ispartof>Infection, genetics and evolution, 2015-12, Vol.36, p.524-530</ispartof><rights>2015</rights><rights>Copyright © 2015. 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Elodie</creatorcontrib><creatorcontrib>Descottes-Genon, Cécile</creatorcontrib><creatorcontrib>Hoen, Bruno</creatorcontrib><creatorcontrib>Patry, Isabelle</creatorcontrib><creatorcontrib>Vettoretti, Lucie</creatorcontrib><creatorcontrib>Chavanet, Pascal</creatorcontrib><creatorcontrib>Eicher, Jean-Christophe</creatorcontrib><creatorcontrib>Gohier-Treuvelot, Sandrine</creatorcontrib><creatorcontrib>Greusard, Marie-Christine</creatorcontrib><creatorcontrib>Neuwirth, Catherine</creatorcontrib><creatorcontrib>Péchinot, André</creatorcontrib><creatorcontrib>Piroth, Lionel</creatorcontrib><creatorcontrib>Célard, Marie</creatorcontrib><creatorcontrib>Cornu, Catherine</creatorcontrib><creatorcontrib>Delahaye, François</creatorcontrib><creatorcontrib>Hadid, Malika</creatorcontrib><creatorcontrib>Rausch, Pascale</creatorcontrib><creatorcontrib>Coma, Audrey</creatorcontrib><creatorcontrib>Galtier, Florence</creatorcontrib><creatorcontrib>Géraud, Philippe</creatorcontrib><creatorcontrib>Jean-Pierre, Hélène</creatorcontrib><creatorcontrib>Le Moing, Vincent</creatorcontrib><creatorcontrib>Sportouch, Catherine</creatorcontrib><creatorcontrib>Reynes, Jacques</creatorcontrib><creatorcontrib>Aissa, Nejla</creatorcontrib><creatorcontrib>Doco-Lecompte, Thanh</creatorcontrib><creatorcontrib>Goehringer, François</creatorcontrib><creatorcontrib>Keil, Nathalie</creatorcontrib><creatorcontrib>Letranchant, Lorraine</creatorcontrib><creatorcontrib>Malela, Hepher</creatorcontrib><creatorcontrib>May, Thierry</creatorcontrib><creatorcontrib>Selton-Suty, Christine</creatorcontrib><creatorcontrib>Bedos, Nathalie</creatorcontrib><creatorcontrib>Lavigne, Jean-Philippe</creatorcontrib><creatorcontrib>Lechiche, Catherine</creatorcontrib><creatorcontrib>Sotto, Albert</creatorcontrib><creatorcontrib>Duval, Xavier</creatorcontrib><creatorcontrib>Habensus, Emila Ilic</creatorcontrib><creatorcontrib>Iung, Bernard</creatorcontrib><creatorcontrib>Leport, Catherine</creatorcontrib><creatorcontrib>Longuet, Pascale</creatorcontrib><creatorcontrib>Ruimy, Raymond</creatorcontrib><creatorcontrib>Bellissant, Eric</creatorcontrib><creatorcontrib>Donnio, Pierre-Yves</creatorcontrib><creatorcontrib>Le Gac, Fabienne</creatorcontrib><creatorcontrib>Michelet, Christian</creatorcontrib><creatorcontrib>Revest, Matthieu</creatorcontrib><creatorcontrib>Tattevin, Pierre</creatorcontrib><creatorcontrib>Thebault, Elise</creatorcontrib><creatorcontrib>Alla, François</creatorcontrib><creatorcontrib>Braquet, Pierre</creatorcontrib><creatorcontrib>Erpelding, Marie-Line</creatorcontrib><creatorcontrib>Minary, Laetitia</creatorcontrib><creatorcontrib>Tubiana, Sarah</creatorcontrib><creatorcontrib>Bès, Michèle</creatorcontrib><creatorcontrib>Etienne, Jérôme</creatorcontrib><creatorcontrib>Tristan, Anne</creatorcontrib><creatorcontrib>Vandenesch, François</creatorcontrib><creatorcontrib>Barbas, Sandrine</creatorcontrib><creatorcontrib>Delonca, Christine</creatorcontrib><creatorcontrib>Sussmuth, Virginie</creatorcontrib><creatorcontrib>Verchère, Anne</creatorcontrib><creatorcontrib>The VIRSTA Study Group</creatorcontrib><creatorcontrib>the French VIRSTA Study Group</creatorcontrib><creatorcontrib>French VIRSTA Study Group</creatorcontrib><title>Staphylococcus aureus infective endocarditis versus bacteremia strains: Subtle genetic differences at stake</title><title>Infection, genetics and evolution</title><addtitle>Infect Genet Evol</addtitle><description>Infective endocarditis (IE)(1) is a severe condition complicating 10–25% of Staphylococcus aureus bacteremia. Although host-related IE risk factors have been identified, the involvement of bacterial features in IE complication is still unclear. We characterized strictly defined IE and bacteremia isolates and searched for discriminant features. S. aureus isolates causing community-acquired, definite native-valve IE (n=72) and bacteremia (n=54) were collected prospectively as part of a French multicenter cohort. Phenotypic traits previously reported or hypothesized to be involved in staphylococcal IE pathogenesis were tested. In parallel, the genotypic profiles of all isolates, obtained by microarray, were analyzed by discriminant analysis of principal components (DAPC)(2). No significant difference was observed between IE and bacteremia strains, regarding either phenotypic or genotypic univariate analyses. However, the multivariate statistical tool DAPC, applied on microarray data, segregated IE and bacteremia isolates: IE isolates were correctly reassigned as such in 80.6% of the cases (C-statistic 0.83, P<0.001). The performance of this model was confirmed with an independent French collection IE and bacteremia isolates (78.8% reassignment, C-statistic 0.65, P<0.01). Finally, a simple linear discriminant function based on a subset of 8 genetic markers retained valuable performance both in study collection (86.1%, P<0.001) and in the independent validation collection (81.8%, P<0.01). We here show that community-acquired IE and bacteremia S. aureus isolates are genetically distinct based on subtle combinations of genetic markers. This finding provides the proof of concept that bacterial characteristics may contribute to the occurrence of IE in patients with S. aureus bacteremia.
•Infective endocarditis (IE) and bacteremia (Ba) S. aureus isolates are collected.•We search for any features discriminating IE and Ba isolates.•No single phenotypic or genotypic trait discriminates the two S. aureus groups.•Multivariate analysis shows subtle genetic differences between IE and Ba isolates.•Results are confirmed on an independent collection of S. aureus isolates.</description><subject>Bacteremia</subject><subject>Bacteremia - microbiology</subject><subject>Community-Acquired Infections</subject><subject>DAPC</subject><subject>Endocarditis, Bacterial - microbiology</subject><subject>Genes, Bacterial</subject><subject>Genetic distinction</subject><subject>Genetic Markers</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Human health and pathology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Infective endocarditis</subject><subject>Life Sciences</subject><subject>Phenotype</subject><subject>Reproducibility 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B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-9412-7106</orcidid><orcidid>https://orcid.org/0000-0001-6911-4362</orcidid><orcidid>https://orcid.org/0000-0001-6297-3543</orcidid><orcidid>https://orcid.org/0000-0002-9236-7998</orcidid></search><sort><creationdate>20151201</creationdate><title>Staphylococcus aureus infective endocarditis versus bacteremia strains: Subtle genetic differences at stake</title><author>Bouchiat, Coralie ; Moreau, Karen ; Devillard, Sébastien ; Rasigade, Jean-Philippe ; Mosnier, Amandine ; Geissmann, Tom ; Bes, Michèle ; Tristan, Anne ; Lina, Gérard ; Laurent, Frédéric ; Piroth, Lionel ; Aissa, Nejla ; Duval, Xavier ; Le Moing, Vincent ; Vandenesch, François ; Chirouze, Catherine ; Curlier, Elodie ; Descottes-Genon, Cécile ; Hoen, Bruno ; Patry, Isabelle ; Vettoretti, Lucie ; Chavanet, Pascal ; Eicher, Jean-Christophe ; Gohier-Treuvelot, Sandrine ; Greusard, Marie-Christine ; Neuwirth, Catherine ; Péchinot, André ; Piroth, Lionel ; Célard, Marie ; Cornu, Catherine ; Delahaye, François ; Hadid, Malika ; Rausch, Pascale ; Coma, Audrey ; Galtier, Florence ; Géraud, Philippe ; Jean-Pierre, Hélène ; Le Moing, Vincent ; Sportouch, Catherine ; Reynes, Jacques ; Aissa, Nejla ; Doco-Lecompte, Thanh ; Goehringer, François ; Keil, Nathalie ; Letranchant, Lorraine ; Malela, Hepher ; May, Thierry ; Selton-Suty, Christine ; Bedos, Nathalie ; Lavigne, Jean-Philippe ; Lechiche, Catherine ; Sotto, Albert ; Duval, Xavier ; Habensus, Emila Ilic ; Iung, Bernard ; Leport, Catherine ; Longuet, Pascale ; Ruimy, Raymond ; Bellissant, Eric ; Donnio, Pierre-Yves ; Le Gac, Fabienne ; Michelet, Christian ; Revest, Matthieu ; Tattevin, Pierre ; Thebault, Elise ; Alla, François ; Braquet, Pierre ; Erpelding, Marie-Line ; Minary, Laetitia ; Tubiana, Sarah ; Bès, Michèle ; Etienne, Jérôme ; Tristan, Anne ; Vandenesch, François ; Barbas, Sandrine ; Delonca, Christine ; Sussmuth, Virginie ; Verchère, Anne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-cc9033947a2dcaec339f20782eb87b3b8abc7b8c220709670af42222073901fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bacteremia</topic><topic>Bacteremia - microbiology</topic><topic>Community-Acquired Infections</topic><topic>DAPC</topic><topic>Endocarditis, Bacterial - microbiology</topic><topic>Genes, Bacterial</topic><topic>Genetic distinction</topic><topic>Genetic Markers</topic><topic>Genetic Variation</topic><topic>Genotype</topic><topic>Human health and pathology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Infective endocarditis</topic><topic>Life Sciences</topic><topic>Phenotype</topic><topic>Reproducibility of Results</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - classification</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bouchiat, Coralie</creatorcontrib><creatorcontrib>Moreau, Karen</creatorcontrib><creatorcontrib>Devillard, Sébastien</creatorcontrib><creatorcontrib>Rasigade, Jean-Philippe</creatorcontrib><creatorcontrib>Mosnier, Amandine</creatorcontrib><creatorcontrib>Geissmann, Tom</creatorcontrib><creatorcontrib>Bes, Michèle</creatorcontrib><creatorcontrib>Tristan, Anne</creatorcontrib><creatorcontrib>Lina, Gérard</creatorcontrib><creatorcontrib>Laurent, Frédéric</creatorcontrib><creatorcontrib>Piroth, Lionel</creatorcontrib><creatorcontrib>Aissa, Nejla</creatorcontrib><creatorcontrib>Duval, Xavier</creatorcontrib><creatorcontrib>Le Moing, Vincent</creatorcontrib><creatorcontrib>Vandenesch, François</creatorcontrib><creatorcontrib>Chirouze, Catherine</creatorcontrib><creatorcontrib>Curlier, Elodie</creatorcontrib><creatorcontrib>Descottes-Genon, Cécile</creatorcontrib><creatorcontrib>Hoen, Bruno</creatorcontrib><creatorcontrib>Patry, Isabelle</creatorcontrib><creatorcontrib>Vettoretti, Lucie</creatorcontrib><creatorcontrib>Chavanet, Pascal</creatorcontrib><creatorcontrib>Eicher, Jean-Christophe</creatorcontrib><creatorcontrib>Gohier-Treuvelot, Sandrine</creatorcontrib><creatorcontrib>Greusard, Marie-Christine</creatorcontrib><creatorcontrib>Neuwirth, Catherine</creatorcontrib><creatorcontrib>Péchinot, André</creatorcontrib><creatorcontrib>Piroth, Lionel</creatorcontrib><creatorcontrib>Célard, Marie</creatorcontrib><creatorcontrib>Cornu, Catherine</creatorcontrib><creatorcontrib>Delahaye, François</creatorcontrib><creatorcontrib>Hadid, Malika</creatorcontrib><creatorcontrib>Rausch, Pascale</creatorcontrib><creatorcontrib>Coma, Audrey</creatorcontrib><creatorcontrib>Galtier, Florence</creatorcontrib><creatorcontrib>Géraud, Philippe</creatorcontrib><creatorcontrib>Jean-Pierre, Hélène</creatorcontrib><creatorcontrib>Le Moing, Vincent</creatorcontrib><creatorcontrib>Sportouch, Catherine</creatorcontrib><creatorcontrib>Reynes, Jacques</creatorcontrib><creatorcontrib>Aissa, Nejla</creatorcontrib><creatorcontrib>Doco-Lecompte, Thanh</creatorcontrib><creatorcontrib>Goehringer, François</creatorcontrib><creatorcontrib>Keil, Nathalie</creatorcontrib><creatorcontrib>Letranchant, Lorraine</creatorcontrib><creatorcontrib>Malela, Hepher</creatorcontrib><creatorcontrib>May, Thierry</creatorcontrib><creatorcontrib>Selton-Suty, Christine</creatorcontrib><creatorcontrib>Bedos, Nathalie</creatorcontrib><creatorcontrib>Lavigne, Jean-Philippe</creatorcontrib><creatorcontrib>Lechiche, Catherine</creatorcontrib><creatorcontrib>Sotto, Albert</creatorcontrib><creatorcontrib>Duval, Xavier</creatorcontrib><creatorcontrib>Habensus, Emila Ilic</creatorcontrib><creatorcontrib>Iung, Bernard</creatorcontrib><creatorcontrib>Leport, Catherine</creatorcontrib><creatorcontrib>Longuet, Pascale</creatorcontrib><creatorcontrib>Ruimy, Raymond</creatorcontrib><creatorcontrib>Bellissant, Eric</creatorcontrib><creatorcontrib>Donnio, Pierre-Yves</creatorcontrib><creatorcontrib>Le Gac, Fabienne</creatorcontrib><creatorcontrib>Michelet, Christian</creatorcontrib><creatorcontrib>Revest, Matthieu</creatorcontrib><creatorcontrib>Tattevin, Pierre</creatorcontrib><creatorcontrib>Thebault, Elise</creatorcontrib><creatorcontrib>Alla, François</creatorcontrib><creatorcontrib>Braquet, Pierre</creatorcontrib><creatorcontrib>Erpelding, Marie-Line</creatorcontrib><creatorcontrib>Minary, Laetitia</creatorcontrib><creatorcontrib>Tubiana, Sarah</creatorcontrib><creatorcontrib>Bès, Michèle</creatorcontrib><creatorcontrib>Etienne, Jérôme</creatorcontrib><creatorcontrib>Tristan, Anne</creatorcontrib><creatorcontrib>Vandenesch, François</creatorcontrib><creatorcontrib>Barbas, Sandrine</creatorcontrib><creatorcontrib>Delonca, Christine</creatorcontrib><creatorcontrib>Sussmuth, Virginie</creatorcontrib><creatorcontrib>Verchère, Anne</creatorcontrib><creatorcontrib>The VIRSTA Study Group</creatorcontrib><creatorcontrib>the French VIRSTA Study Group</creatorcontrib><creatorcontrib>French VIRSTA Study Group</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Infection, genetics and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bouchiat, Coralie</au><au>Moreau, Karen</au><au>Devillard, Sébastien</au><au>Rasigade, Jean-Philippe</au><au>Mosnier, Amandine</au><au>Geissmann, Tom</au><au>Bes, Michèle</au><au>Tristan, Anne</au><au>Lina, Gérard</au><au>Laurent, Frédéric</au><au>Piroth, Lionel</au><au>Aissa, Nejla</au><au>Duval, Xavier</au><au>Le Moing, Vincent</au><au>Vandenesch, François</au><au>Chirouze, Catherine</au><au>Curlier, Elodie</au><au>Descottes-Genon, Cécile</au><au>Hoen, Bruno</au><au>Patry, Isabelle</au><au>Vettoretti, Lucie</au><au>Chavanet, Pascal</au><au>Eicher, Jean-Christophe</au><au>Gohier-Treuvelot, Sandrine</au><au>Greusard, Marie-Christine</au><au>Neuwirth, Catherine</au><au>Péchinot, André</au><au>Piroth, Lionel</au><au>Célard, Marie</au><au>Cornu, Catherine</au><au>Delahaye, François</au><au>Hadid, Malika</au><au>Rausch, Pascale</au><au>Coma, Audrey</au><au>Galtier, Florence</au><au>Géraud, Philippe</au><au>Jean-Pierre, Hélène</au><au>Le Moing, Vincent</au><au>Sportouch, Catherine</au><au>Reynes, Jacques</au><au>Aissa, Nejla</au><au>Doco-Lecompte, Thanh</au><au>Goehringer, François</au><au>Keil, Nathalie</au><au>Letranchant, Lorraine</au><au>Malela, Hepher</au><au>May, Thierry</au><au>Selton-Suty, Christine</au><au>Bedos, Nathalie</au><au>Lavigne, Jean-Philippe</au><au>Lechiche, Catherine</au><au>Sotto, Albert</au><au>Duval, Xavier</au><au>Habensus, Emila Ilic</au><au>Iung, Bernard</au><au>Leport, Catherine</au><au>Longuet, Pascale</au><au>Ruimy, Raymond</au><au>Bellissant, Eric</au><au>Donnio, Pierre-Yves</au><au>Le Gac, Fabienne</au><au>Michelet, Christian</au><au>Revest, Matthieu</au><au>Tattevin, Pierre</au><au>Thebault, Elise</au><au>Alla, François</au><au>Braquet, Pierre</au><au>Erpelding, Marie-Line</au><au>Minary, Laetitia</au><au>Tubiana, Sarah</au><au>Bès, Michèle</au><au>Etienne, Jérôme</au><au>Tristan, Anne</au><au>Vandenesch, François</au><au>Barbas, Sandrine</au><au>Delonca, Christine</au><au>Sussmuth, Virginie</au><au>Verchère, Anne</au><aucorp>The VIRSTA Study Group</aucorp><aucorp>the French VIRSTA Study Group</aucorp><aucorp>French VIRSTA Study Group</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Staphylococcus aureus infective endocarditis versus bacteremia strains: Subtle genetic differences at stake</atitle><jtitle>Infection, genetics and evolution</jtitle><addtitle>Infect Genet Evol</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>36</volume><spage>524</spage><epage>530</epage><pages>524-530</pages><issn>1567-1348</issn><eissn>1567-7257</eissn><abstract>Infective endocarditis (IE)(1) is a severe condition complicating 10–25% of Staphylococcus aureus bacteremia. Although host-related IE risk factors have been identified, the involvement of bacterial features in IE complication is still unclear. We characterized strictly defined IE and bacteremia isolates and searched for discriminant features. S. aureus isolates causing community-acquired, definite native-valve IE (n=72) and bacteremia (n=54) were collected prospectively as part of a French multicenter cohort. Phenotypic traits previously reported or hypothesized to be involved in staphylococcal IE pathogenesis were tested. In parallel, the genotypic profiles of all isolates, obtained by microarray, were analyzed by discriminant analysis of principal components (DAPC)(2). No significant difference was observed between IE and bacteremia strains, regarding either phenotypic or genotypic univariate analyses. However, the multivariate statistical tool DAPC, applied on microarray data, segregated IE and bacteremia isolates: IE isolates were correctly reassigned as such in 80.6% of the cases (C-statistic 0.83, P<0.001). The performance of this model was confirmed with an independent French collection IE and bacteremia isolates (78.8% reassignment, C-statistic 0.65, P<0.01). Finally, a simple linear discriminant function based on a subset of 8 genetic markers retained valuable performance both in study collection (86.1%, P<0.001) and in the independent validation collection (81.8%, P<0.01). We here show that community-acquired IE and bacteremia S. aureus isolates are genetically distinct based on subtle combinations of genetic markers. This finding provides the proof of concept that bacterial characteristics may contribute to the occurrence of IE in patients with S. aureus bacteremia.
•Infective endocarditis (IE) and bacteremia (Ba) S. aureus isolates are collected.•We search for any features discriminating IE and Ba isolates.•No single phenotypic or genotypic trait discriminates the two S. aureus groups.•Multivariate analysis shows subtle genetic differences between IE and Ba isolates.•Results are confirmed on an independent collection of S. aureus isolates.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26318542</pmid><doi>10.1016/j.meegid.2015.08.029</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-9412-7106</orcidid><orcidid>https://orcid.org/0000-0001-6911-4362</orcidid><orcidid>https://orcid.org/0000-0001-6297-3543</orcidid><orcidid>https://orcid.org/0000-0002-9236-7998</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1567-1348 |
| ispartof | Infection, genetics and evolution, 2015-12, Vol.36, p.524-530 |
| issn | 1567-1348 1567-7257 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_01913333v1 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Bacteremia Bacteremia - microbiology Community-Acquired Infections DAPC Endocarditis, Bacterial - microbiology Genes, Bacterial Genetic distinction Genetic Markers Genetic Variation Genotype Human health and pathology Humans Infectious diseases Infective endocarditis Life Sciences Phenotype Reproducibility of Results Staphylococcal Infections - microbiology Staphylococcus aureus Staphylococcus aureus - classification Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification |
| title | Staphylococcus aureus infective endocarditis versus bacteremia strains: Subtle genetic differences at stake |
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