Natural infection of Ctenodactylus gundi by Leishmania major in Tunisia
[Display omitted] •Confirmed Leishmania infection in Ctenodactylus gundi.•We detect Leishmania with clinical, parasitological and molecular tools.•The molecular methods used allow identifying the species involved as Leishmania major and Leishmania tropica.•Ctenodactylus gundi is a potential reservoi...
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| Veröffentlicht in: | Acta tropica 2018-01, Vol.177, p.89-93 |
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| creator | Ghawar, Wissem Bettaieb, Jihène Salem, Sadok Snoussi, Mohammed-Ali Jaouadi, Kaouther Yazidi, Rihab Ben-Salah, Afif |
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•Confirmed Leishmania infection in Ctenodactylus gundi.•We detect Leishmania with clinical, parasitological and molecular tools.•The molecular methods used allow identifying the species involved as Leishmania major and Leishmania tropica.•Ctenodactylus gundi is a potential reservoir of Leishmania major in Tunisia.
Incriminating new rodent species, as reservoir hosts of Leishmania parasites is crucial for understanding the transmission cycle of cutaneous leishmaniasis in Tunisia. Ctenodactylus (C.) gundi was previously described as extremely abundant in all Tunisian Leishmania (L.) tropica foci in south Tunisia besides its presence in L. major endemic area. The aim of this study was to detect Leishmania species parasites among C. gundi in two endemic regions in Tunisia: Sidi Bouzid and Tataouine.
Total DNA was isolated from the spleens and the livers of 92C. gundi. Leishmaniasis clinical manifestations were detected among 11 rodents (12%). Leishmania parasites were detected in 30 (32.6%) rodents using direct exam method. Leishmania DNA was detected in 40 (43.5%) C. gundi by combining results among spleens and livers using ITS1-PCR. Positive samples were confirmed to be L. major except for only one specimen which was L. tropica.
These results demonstrated, for the first time, the high natural infection rate of C. gundi with L. major parasites in Tunisia. Hence, C. gundi should be considered as potential reservoir host of Leishmania parasites causing cutaneous leishmaniasis in Tunisia. |
| doi_str_mv | 10.1016/j.actatropica.2017.09.022 |
| format | Article |
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•Confirmed Leishmania infection in Ctenodactylus gundi.•We detect Leishmania with clinical, parasitological and molecular tools.•The molecular methods used allow identifying the species involved as Leishmania major and Leishmania tropica.•Ctenodactylus gundi is a potential reservoir of Leishmania major in Tunisia.
Incriminating new rodent species, as reservoir hosts of Leishmania parasites is crucial for understanding the transmission cycle of cutaneous leishmaniasis in Tunisia. Ctenodactylus (C.) gundi was previously described as extremely abundant in all Tunisian Leishmania (L.) tropica foci in south Tunisia besides its presence in L. major endemic area. The aim of this study was to detect Leishmania species parasites among C. gundi in two endemic regions in Tunisia: Sidi Bouzid and Tataouine.
Total DNA was isolated from the spleens and the livers of 92C. gundi. Leishmaniasis clinical manifestations were detected among 11 rodents (12%). Leishmania parasites were detected in 30 (32.6%) rodents using direct exam method. Leishmania DNA was detected in 40 (43.5%) C. gundi by combining results among spleens and livers using ITS1-PCR. Positive samples were confirmed to be L. major except for only one specimen which was L. tropica.
These results demonstrated, for the first time, the high natural infection rate of C. gundi with L. major parasites in Tunisia. Hence, C. gundi should be considered as potential reservoir host of Leishmania parasites causing cutaneous leishmaniasis in Tunisia.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/j.actatropica.2017.09.022</identifier><identifier>PMID: 28963065</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Ctenodactylus gundi ; Cutaneous leishmaniasis ; Disease Reservoirs - parasitology ; Flea Infestations - parasitology ; Human health and pathology ; Humans ; Leishmania major ; Leishmania major - genetics ; Leishmania major - isolation & purification ; Leishmania tropica ; Leishmania tropica - genetics ; Leishmania tropica - isolation & purification ; Leishmaniasis, Cutaneous - epidemiology ; Leishmaniasis, Cutaneous - parasitology ; Life Sciences ; Rodent reservoir ; Rodentia - parasitology ; Tunisia ; Tunisia - epidemiology ; Zoonoses - parasitology</subject><ispartof>Acta tropica, 2018-01, Vol.177, p.89-93</ispartof><rights>2017</rights><rights>Copyright © 2017. Published by Elsevier B.V.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-85e6a4d152d4baec6e85b72889afa47e906741a5977f98530f89ef601e0e98513</citedby><cites>FETCH-LOGICAL-c411t-85e6a4d152d4baec6e85b72889afa47e906741a5977f98530f89ef601e0e98513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0001706X17300153$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28963065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01876289$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ghawar, Wissem</creatorcontrib><creatorcontrib>Bettaieb, Jihène</creatorcontrib><creatorcontrib>Salem, Sadok</creatorcontrib><creatorcontrib>Snoussi, Mohammed-Ali</creatorcontrib><creatorcontrib>Jaouadi, Kaouther</creatorcontrib><creatorcontrib>Yazidi, Rihab</creatorcontrib><creatorcontrib>Ben-Salah, Afif</creatorcontrib><title>Natural infection of Ctenodactylus gundi by Leishmania major in Tunisia</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>[Display omitted]
•Confirmed Leishmania infection in Ctenodactylus gundi.•We detect Leishmania with clinical, parasitological and molecular tools.•The molecular methods used allow identifying the species involved as Leishmania major and Leishmania tropica.•Ctenodactylus gundi is a potential reservoir of Leishmania major in Tunisia.
Incriminating new rodent species, as reservoir hosts of Leishmania parasites is crucial for understanding the transmission cycle of cutaneous leishmaniasis in Tunisia. Ctenodactylus (C.) gundi was previously described as extremely abundant in all Tunisian Leishmania (L.) tropica foci in south Tunisia besides its presence in L. major endemic area. The aim of this study was to detect Leishmania species parasites among C. gundi in two endemic regions in Tunisia: Sidi Bouzid and Tataouine.
Total DNA was isolated from the spleens and the livers of 92C. gundi. Leishmaniasis clinical manifestations were detected among 11 rodents (12%). Leishmania parasites were detected in 30 (32.6%) rodents using direct exam method. Leishmania DNA was detected in 40 (43.5%) C. gundi by combining results among spleens and livers using ITS1-PCR. Positive samples were confirmed to be L. major except for only one specimen which was L. tropica.
These results demonstrated, for the first time, the high natural infection rate of C. gundi with L. major parasites in Tunisia. Hence, C. gundi should be considered as potential reservoir host of Leishmania parasites causing cutaneous leishmaniasis in Tunisia.</description><subject>Animals</subject><subject>Ctenodactylus gundi</subject><subject>Cutaneous leishmaniasis</subject><subject>Disease Reservoirs - parasitology</subject><subject>Flea Infestations - parasitology</subject><subject>Human health and pathology</subject><subject>Humans</subject><subject>Leishmania major</subject><subject>Leishmania major - genetics</subject><subject>Leishmania major - isolation & purification</subject><subject>Leishmania tropica</subject><subject>Leishmania tropica - genetics</subject><subject>Leishmania tropica - isolation & purification</subject><subject>Leishmaniasis, Cutaneous - epidemiology</subject><subject>Leishmaniasis, Cutaneous - parasitology</subject><subject>Life Sciences</subject><subject>Rodent reservoir</subject><subject>Rodentia - parasitology</subject><subject>Tunisia</subject><subject>Tunisia - epidemiology</subject><subject>Zoonoses - parasitology</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMFq3DAQhkVJabZpXyE4t_Rgd6S1JOsYljQJLO0lhd7ErDxutNjWRrIX9u2rZdOQY05ixPfPz3yMXXGoOHD1fVuhm3CKYecdVgK4rsBUIMQHtuCNXpZKyPqMLQCAlxrUn3P2OaVtnoSW4hM7F41RS1Bywe5-4jRH7As_duQmH8YidMVqojG0ueTQz6n4O4-tLzaHYk0-PQ04eiwG3IaYQ8XjPPrk8Qv72GGf6OvLe8F-_7h9XN2X6193D6ubdelqzqeykaSwbrkUbb1BcooaudGiaQx2WGsyoHTNURqtO9PIJXSNoU4BJ6A88-UF-3ba-4S93UU_YDzYgN7e36zt8Q-yAJXv2x_Z6xO7i-F5pjTZwSdHfY8jhTlZbmopuMrlGTUn1MWQUqTudTcHe3Rut_aNc3t0bsHY7DxnL19q5s1A7Wvyv-QMrE4AZTF7T9Em52l01PqYnds2-HfU_ANl95d9</recordid><startdate>201801</startdate><enddate>201801</enddate><creator>Ghawar, Wissem</creator><creator>Bettaieb, Jihène</creator><creator>Salem, Sadok</creator><creator>Snoussi, Mohammed-Ali</creator><creator>Jaouadi, Kaouther</creator><creator>Yazidi, Rihab</creator><creator>Ben-Salah, Afif</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>201801</creationdate><title>Natural infection of Ctenodactylus gundi by Leishmania major in Tunisia</title><author>Ghawar, Wissem ; Bettaieb, Jihène ; Salem, Sadok ; Snoussi, Mohammed-Ali ; Jaouadi, Kaouther ; Yazidi, Rihab ; Ben-Salah, Afif</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-85e6a4d152d4baec6e85b72889afa47e906741a5977f98530f89ef601e0e98513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Ctenodactylus gundi</topic><topic>Cutaneous leishmaniasis</topic><topic>Disease Reservoirs - parasitology</topic><topic>Flea Infestations - parasitology</topic><topic>Human health and pathology</topic><topic>Humans</topic><topic>Leishmania major</topic><topic>Leishmania major - genetics</topic><topic>Leishmania major - isolation & purification</topic><topic>Leishmania tropica</topic><topic>Leishmania tropica - genetics</topic><topic>Leishmania tropica - isolation & purification</topic><topic>Leishmaniasis, Cutaneous - epidemiology</topic><topic>Leishmaniasis, Cutaneous - parasitology</topic><topic>Life Sciences</topic><topic>Rodent reservoir</topic><topic>Rodentia - parasitology</topic><topic>Tunisia</topic><topic>Tunisia - epidemiology</topic><topic>Zoonoses - parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghawar, Wissem</creatorcontrib><creatorcontrib>Bettaieb, Jihène</creatorcontrib><creatorcontrib>Salem, Sadok</creatorcontrib><creatorcontrib>Snoussi, Mohammed-Ali</creatorcontrib><creatorcontrib>Jaouadi, Kaouther</creatorcontrib><creatorcontrib>Yazidi, Rihab</creatorcontrib><creatorcontrib>Ben-Salah, Afif</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghawar, Wissem</au><au>Bettaieb, Jihène</au><au>Salem, Sadok</au><au>Snoussi, Mohammed-Ali</au><au>Jaouadi, Kaouther</au><au>Yazidi, Rihab</au><au>Ben-Salah, Afif</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Natural infection of Ctenodactylus gundi by Leishmania major in Tunisia</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>2018-01</date><risdate>2018</risdate><volume>177</volume><spage>89</spage><epage>93</epage><pages>89-93</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><abstract>[Display omitted]
•Confirmed Leishmania infection in Ctenodactylus gundi.•We detect Leishmania with clinical, parasitological and molecular tools.•The molecular methods used allow identifying the species involved as Leishmania major and Leishmania tropica.•Ctenodactylus gundi is a potential reservoir of Leishmania major in Tunisia.
Incriminating new rodent species, as reservoir hosts of Leishmania parasites is crucial for understanding the transmission cycle of cutaneous leishmaniasis in Tunisia. Ctenodactylus (C.) gundi was previously described as extremely abundant in all Tunisian Leishmania (L.) tropica foci in south Tunisia besides its presence in L. major endemic area. The aim of this study was to detect Leishmania species parasites among C. gundi in two endemic regions in Tunisia: Sidi Bouzid and Tataouine.
Total DNA was isolated from the spleens and the livers of 92C. gundi. Leishmaniasis clinical manifestations were detected among 11 rodents (12%). Leishmania parasites were detected in 30 (32.6%) rodents using direct exam method. Leishmania DNA was detected in 40 (43.5%) C. gundi by combining results among spleens and livers using ITS1-PCR. Positive samples were confirmed to be L. major except for only one specimen which was L. tropica.
These results demonstrated, for the first time, the high natural infection rate of C. gundi with L. major parasites in Tunisia. Hence, C. gundi should be considered as potential reservoir host of Leishmania parasites causing cutaneous leishmaniasis in Tunisia.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28963065</pmid><doi>10.1016/j.actatropica.2017.09.022</doi><tpages>5</tpages></addata></record> |
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| ispartof | Acta tropica, 2018-01, Vol.177, p.89-93 |
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| subjects | Animals Ctenodactylus gundi Cutaneous leishmaniasis Disease Reservoirs - parasitology Flea Infestations - parasitology Human health and pathology Humans Leishmania major Leishmania major - genetics Leishmania major - isolation & purification Leishmania tropica Leishmania tropica - genetics Leishmania tropica - isolation & purification Leishmaniasis, Cutaneous - epidemiology Leishmaniasis, Cutaneous - parasitology Life Sciences Rodent reservoir Rodentia - parasitology Tunisia Tunisia - epidemiology Zoonoses - parasitology |
| title | Natural infection of Ctenodactylus gundi by Leishmania major in Tunisia |
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