In vitro translation in a hybrid cell free lysate with exogenous cellular ribosomes
Cell free protein synthesis systems (CFPS) have been widely used to express proteins and to explore the pathways of gene expression. In the present manuscript, we describe the design of a novel adaptable hybrid in vitro translation system which is assembled with ribosomes isolated from many differen...
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| Veröffentlicht in: | Biochemical journal 2015-05, Vol.467 (3), p.387-398 |
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| container_end_page | 398 |
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| container_issue | 3 |
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| container_title | Biochemical journal |
| container_volume | 467 |
| creator | Panthu, Baptiste Décimo, Didier Balvay, Laurent Ohlmann, Théophile |
| description | Cell free protein synthesis systems (CFPS) have been widely used to express proteins and to explore the pathways of gene expression. In the present manuscript, we describe the design of a novel adaptable hybrid in vitro translation system which is assembled with ribosomes isolated from many different origins. We first show that this hybrid system exhibits all important features such as efficiency, sensitivity, reproducibility and the ability to translate specialized mRNAs in less than 1 h. In addition, the unique design of this cell free assay makes it highly adaptable to utilize ribosomes isolated from many different organs, tissues or cell types. |
| doi_str_mv | 10.1042/BJ20141498 |
| format | Article |
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In addition, the unique design of this cell free assay makes it highly adaptable to utilize ribosomes isolated from many different organs, tissues or cell types.</description><subject>5' Untranslated Regions</subject><subject>Animals</subject><subject>beta-Globins - biosynthesis</subject><subject>beta-Globins - genetics</subject><subject>Cell Line</subject><subject>Cell-Free System</subject><subject>Cells, Cultured</subject><subject>Cellular Biology</subject><subject>Cricetinae</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Jurkat Cells</subject><subject>Life Sciences</subject><subject>Luciferases, Renilla - biosynthesis</subject><subject>Luciferases, Renilla - genetics</subject><subject>Mice</subject><subject>Poliovirus - genetics</subject><subject>Protein Biosynthesis - genetics</subject><subject>Rabbits</subject><subject>Reticulocytes - metabolism</subject><subject>Ribosomes - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>Subcellular Processes</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFOwzAQRC0EoqVw4QOQj4AU8Dp27B5LBbSoEpdyjjaNQ43SuNhJoX_Dt_BlpLSU00qzT6OZIeQc2A0wwW_vnjgDAaKvD0gXhGKRVlwfki7jiYgSxqFDTkJ4Yy3FBDsmHS4TrhnoLpmOq--vla29o7XHKpRYW1dRW1Gk83XmbU5npixp4Y2h5TpgbeiHrefUfLpXU7km_P6bEj31NnPBLUw4JUcFlsGc7W6PvDzcT4ejaPL8OB4OJtEs5lBHhikps7zfL4QChaIokBUGY51AoXVfixhAM9EKKJQySqu8DQ6co9SAMcY9crX1nWOZLr1doF-nDm06GkzSjdZWVCClXEHLXm7ZpXfvjQl1urBhEx0r07ZIIVGCMyk1b9HrLTrzLgRvir03sHSzePq_eAtf7HybbGHyPfo3cfwDuDp6CQ</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Panthu, Baptiste</creator><creator>Décimo, Didier</creator><creator>Balvay, Laurent</creator><creator>Ohlmann, Théophile</creator><general>Portland Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-9430-4061</orcidid><orcidid>https://orcid.org/0000-0002-2001-8916</orcidid><orcidid>https://orcid.org/0000-0002-0390-1205</orcidid></search><sort><creationdate>20150501</creationdate><title>In vitro translation in a hybrid cell free lysate with exogenous cellular ribosomes</title><author>Panthu, Baptiste ; Décimo, Didier ; Balvay, Laurent ; Ohlmann, Théophile</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c321t-e0755bd99f4717a4ffa0fea3861f88984311804a38a477e787d256122a581a3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>5' Untranslated Regions</topic><topic>Animals</topic><topic>beta-Globins - biosynthesis</topic><topic>beta-Globins - genetics</topic><topic>Cell Line</topic><topic>Cell-Free System</topic><topic>Cells, Cultured</topic><topic>Cellular Biology</topic><topic>Cricetinae</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Jurkat Cells</topic><topic>Life Sciences</topic><topic>Luciferases, Renilla - biosynthesis</topic><topic>Luciferases, Renilla - genetics</topic><topic>Mice</topic><topic>Poliovirus - genetics</topic><topic>Protein Biosynthesis - genetics</topic><topic>Rabbits</topic><topic>Reticulocytes - metabolism</topic><topic>Ribosomes - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>Subcellular Processes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Panthu, Baptiste</creatorcontrib><creatorcontrib>Décimo, Didier</creatorcontrib><creatorcontrib>Balvay, Laurent</creatorcontrib><creatorcontrib>Ohlmann, Théophile</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Panthu, Baptiste</au><au>Décimo, Didier</au><au>Balvay, Laurent</au><au>Ohlmann, Théophile</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro translation in a hybrid cell free lysate with exogenous cellular ribosomes</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>467</volume><issue>3</issue><spage>387</spage><epage>398</epage><pages>387-398</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>Cell free protein synthesis systems (CFPS) have been widely used to express proteins and to explore the pathways of gene expression. 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| ispartof | Biochemical journal, 2015-05, Vol.467 (3), p.387-398 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | 5' Untranslated Regions Animals beta-Globins - biosynthesis beta-Globins - genetics Cell Line Cell-Free System Cells, Cultured Cellular Biology Cricetinae HeLa Cells Humans In Vitro Techniques Jurkat Cells Life Sciences Luciferases, Renilla - biosynthesis Luciferases, Renilla - genetics Mice Poliovirus - genetics Protein Biosynthesis - genetics Rabbits Reticulocytes - metabolism Ribosomes - metabolism RNA, Messenger - genetics Subcellular Processes |
| title | In vitro translation in a hybrid cell free lysate with exogenous cellular ribosomes |
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