Formation of polyelectrolyte complexes with diethylaminoethyl dextran: Charge ratio and molar mass effect
•Anionic and cationic polyelectrolyte based on polysaccharide complexes are studied.•Zeta potential and ITC measurements show an atypical complexe stoichiometry (0.6).•Deviation from the 1:1 ratio is due to inaccessibility of some cationic charges.•PECs aggregates are evidenced by AsF4/MALS/DRI anal...
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| Veröffentlicht in: | Carbohydrate polymers 2014-11, Vol.113, p.217-224 |
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| creator | Le Cerf, Didier Pepin, Anne Sophie Niang, Pape Momar Cristea, Mariana Karakasyan-Dia, Carole Picton, Luc |
| description | •Anionic and cationic polyelectrolyte based on polysaccharide complexes are studied.•Zeta potential and ITC measurements show an atypical complexe stoichiometry (0.6).•Deviation from the 1:1 ratio is due to inaccessibility of some cationic charges.•PECs aggregates are evidenced by AsF4/MALS/DRI analysis.
The formation of polyelectrolyte complexes (PECs) between carboxymethyl pullulan and DEAE Dextran, was investigated, in dilute solution, with emphasis on the effect of charge density (molar ratio or pH) and molar masses. Electrophoretic mobility measurements have evidenced that insoluble PECs (neutral electrophoretic mobility) occurs for charge ratio between 0.6 (excess of polycation) and 1 (stoichiometry usual value) according to the pH. This atypical result is explained by the inaccessibility of some permanent cationic charge when screened by pH dependant cationic ones (due to the Hoffman alkylation). Isothermal titration calorimetry (ITC) indicates an endothermic formation of PEC with a binding constant around 105Lmol−1. Finally asymmetrical flow field flow fractionation coupled on line with static multi angle light scattering (AF4/MALS) evidences soluble PECs with very large average molar masses and size around 100nm, in agreement with scrambled eggs multi-association between various polyelectrolyte chains. |
| doi_str_mv | 10.1016/j.carbpol.2014.07.015 |
| format | Article |
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The formation of polyelectrolyte complexes (PECs) between carboxymethyl pullulan and DEAE Dextran, was investigated, in dilute solution, with emphasis on the effect of charge density (molar ratio or pH) and molar masses. Electrophoretic mobility measurements have evidenced that insoluble PECs (neutral electrophoretic mobility) occurs for charge ratio between 0.6 (excess of polycation) and 1 (stoichiometry usual value) according to the pH. This atypical result is explained by the inaccessibility of some permanent cationic charge when screened by pH dependant cationic ones (due to the Hoffman alkylation). Isothermal titration calorimetry (ITC) indicates an endothermic formation of PEC with a binding constant around 105Lmol−1. Finally asymmetrical flow field flow fractionation coupled on line with static multi angle light scattering (AF4/MALS) evidences soluble PECs with very large average molar masses and size around 100nm, in agreement with scrambled eggs multi-association between various polyelectrolyte chains.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2014.07.015</identifier><identifier>PMID: 25256478</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Applied sciences ; Calorimetry - methods ; Chemical Sciences ; DEAE Dextran ; DEAE-Dextran - chemistry ; DEAE-Dextran - metabolism ; Electrolytes - chemistry ; Electrolytes - metabolism ; Electrophoresis - methods ; Exact sciences and technology ; ITC ; Natural polymers ; Physicochemistry of polymers ; Polyelectrolyte complexes ; Polymers ; Polymers - chemistry ; Polymers - metabolism ; Polysaccharide ; Starch and polysaccharides</subject><ispartof>Carbohydrate polymers, 2014-11, Vol.113, p.217-224</ispartof><rights>2014 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-f5c300c61694355fd0327c79ece2f3ad96f2aebf24ffb8e4f361b0ed8e1fb5ec3</citedby><cites>FETCH-LOGICAL-c429t-f5c300c61694355fd0327c79ece2f3ad96f2aebf24ffb8e4f361b0ed8e1fb5ec3</cites><orcidid>0000-0002-5853-9274 ; 0000-0001-9537-8003</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0144861714006882$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28819080$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25256478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://normandie-univ.hal.science/hal-01866729$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Le Cerf, Didier</creatorcontrib><creatorcontrib>Pepin, Anne Sophie</creatorcontrib><creatorcontrib>Niang, Pape Momar</creatorcontrib><creatorcontrib>Cristea, Mariana</creatorcontrib><creatorcontrib>Karakasyan-Dia, Carole</creatorcontrib><creatorcontrib>Picton, Luc</creatorcontrib><title>Formation of polyelectrolyte complexes with diethylaminoethyl dextran: Charge ratio and molar mass effect</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>•Anionic and cationic polyelectrolyte based on polysaccharide complexes are studied.•Zeta potential and ITC measurements show an atypical complexe stoichiometry (0.6).•Deviation from the 1:1 ratio is due to inaccessibility of some cationic charges.•PECs aggregates are evidenced by AsF4/MALS/DRI analysis.
The formation of polyelectrolyte complexes (PECs) between carboxymethyl pullulan and DEAE Dextran, was investigated, in dilute solution, with emphasis on the effect of charge density (molar ratio or pH) and molar masses. Electrophoretic mobility measurements have evidenced that insoluble PECs (neutral electrophoretic mobility) occurs for charge ratio between 0.6 (excess of polycation) and 1 (stoichiometry usual value) according to the pH. This atypical result is explained by the inaccessibility of some permanent cationic charge when screened by pH dependant cationic ones (due to the Hoffman alkylation). Isothermal titration calorimetry (ITC) indicates an endothermic formation of PEC with a binding constant around 105Lmol−1. Finally asymmetrical flow field flow fractionation coupled on line with static multi angle light scattering (AF4/MALS) evidences soluble PECs with very large average molar masses and size around 100nm, in agreement with scrambled eggs multi-association between various polyelectrolyte chains.</description><subject>Applied sciences</subject><subject>Calorimetry - methods</subject><subject>Chemical Sciences</subject><subject>DEAE Dextran</subject><subject>DEAE-Dextran - chemistry</subject><subject>DEAE-Dextran - metabolism</subject><subject>Electrolytes - chemistry</subject><subject>Electrolytes - metabolism</subject><subject>Electrophoresis - methods</subject><subject>Exact sciences and technology</subject><subject>ITC</subject><subject>Natural polymers</subject><subject>Physicochemistry of polymers</subject><subject>Polyelectrolyte complexes</subject><subject>Polymers</subject><subject>Polymers - chemistry</subject><subject>Polymers - metabolism</subject><subject>Polysaccharide</subject><subject>Starch and polysaccharides</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGPFCEQhYnRuOPqT9BwMdFDt0ADTXvZbCa7rskkXvRMaLpwmNDNCD3rzr9f2hnXo1yoFF-9Iu8h9JaSmhIqP-1qa1K_j6FmhPKatDWh4hlaUdV2FW04f45W5YFXStL2Ar3KeUfKkZS8RBdMMCF5q1bI38Y0mtnHCUeHi9wRAtg5lWIGbOO4D_AAGf_28xYPHubtMZjRT_FPhQd4mJOZPuP11qSfgNMihc004DEGk_BocsbgXJF8jV44EzK8Od-X6Mftzff1XbX59uXr-npTWc66uXLCNoRYSWXHGyHcQBrW2rYDC8w1ZuikYwZ6x7hzvQLuGkl7AoMC6noBtrlEH0-6WxP0PvnRpKOOxuu7641eeoQqKVvW3dPCfjix-xR_HSDPevTZQghmgnjImgopOaFtt6DihNoUc07gnrQp0UsieqfPieglEU3askmUuXfnFYd-hOFp6m8EBXh_Bky2Jrhip_X5H6cU7Ygihbs6cVDMu_eQdLYeJguDT8VePUT_n688Av8Lrjw</recordid><startdate>20141126</startdate><enddate>20141126</enddate><creator>Le Cerf, Didier</creator><creator>Pepin, Anne Sophie</creator><creator>Niang, Pape Momar</creator><creator>Cristea, Mariana</creator><creator>Karakasyan-Dia, Carole</creator><creator>Picton, Luc</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-5853-9274</orcidid><orcidid>https://orcid.org/0000-0001-9537-8003</orcidid></search><sort><creationdate>20141126</creationdate><title>Formation of polyelectrolyte complexes with diethylaminoethyl dextran: Charge ratio and molar mass effect</title><author>Le Cerf, Didier ; Pepin, Anne Sophie ; Niang, Pape Momar ; Cristea, Mariana ; Karakasyan-Dia, Carole ; Picton, Luc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-f5c300c61694355fd0327c79ece2f3ad96f2aebf24ffb8e4f361b0ed8e1fb5ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Applied sciences</topic><topic>Calorimetry - methods</topic><topic>Chemical Sciences</topic><topic>DEAE Dextran</topic><topic>DEAE-Dextran - chemistry</topic><topic>DEAE-Dextran - metabolism</topic><topic>Electrolytes - chemistry</topic><topic>Electrolytes - metabolism</topic><topic>Electrophoresis - methods</topic><topic>Exact sciences and technology</topic><topic>ITC</topic><topic>Natural polymers</topic><topic>Physicochemistry of polymers</topic><topic>Polyelectrolyte complexes</topic><topic>Polymers</topic><topic>Polymers - chemistry</topic><topic>Polymers - metabolism</topic><topic>Polysaccharide</topic><topic>Starch and polysaccharides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Le Cerf, Didier</creatorcontrib><creatorcontrib>Pepin, Anne Sophie</creatorcontrib><creatorcontrib>Niang, Pape Momar</creatorcontrib><creatorcontrib>Cristea, Mariana</creatorcontrib><creatorcontrib>Karakasyan-Dia, Carole</creatorcontrib><creatorcontrib>Picton, Luc</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Le Cerf, Didier</au><au>Pepin, Anne Sophie</au><au>Niang, Pape Momar</au><au>Cristea, Mariana</au><au>Karakasyan-Dia, Carole</au><au>Picton, Luc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Formation of polyelectrolyte complexes with diethylaminoethyl dextran: Charge ratio and molar mass effect</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2014-11-26</date><risdate>2014</risdate><volume>113</volume><spage>217</spage><epage>224</epage><pages>217-224</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>•Anionic and cationic polyelectrolyte based on polysaccharide complexes are studied.•Zeta potential and ITC measurements show an atypical complexe stoichiometry (0.6).•Deviation from the 1:1 ratio is due to inaccessibility of some cationic charges.•PECs aggregates are evidenced by AsF4/MALS/DRI analysis.
The formation of polyelectrolyte complexes (PECs) between carboxymethyl pullulan and DEAE Dextran, was investigated, in dilute solution, with emphasis on the effect of charge density (molar ratio or pH) and molar masses. Electrophoretic mobility measurements have evidenced that insoluble PECs (neutral electrophoretic mobility) occurs for charge ratio between 0.6 (excess of polycation) and 1 (stoichiometry usual value) according to the pH. This atypical result is explained by the inaccessibility of some permanent cationic charge when screened by pH dependant cationic ones (due to the Hoffman alkylation). Isothermal titration calorimetry (ITC) indicates an endothermic formation of PEC with a binding constant around 105Lmol−1. Finally asymmetrical flow field flow fractionation coupled on line with static multi angle light scattering (AF4/MALS) evidences soluble PECs with very large average molar masses and size around 100nm, in agreement with scrambled eggs multi-association between various polyelectrolyte chains.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>25256478</pmid><doi>10.1016/j.carbpol.2014.07.015</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-5853-9274</orcidid><orcidid>https://orcid.org/0000-0001-9537-8003</orcidid></addata></record> |
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| ispartof | Carbohydrate polymers, 2014-11, Vol.113, p.217-224 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Applied sciences Calorimetry - methods Chemical Sciences DEAE Dextran DEAE-Dextran - chemistry DEAE-Dextran - metabolism Electrolytes - chemistry Electrolytes - metabolism Electrophoresis - methods Exact sciences and technology ITC Natural polymers Physicochemistry of polymers Polyelectrolyte complexes Polymers Polymers - chemistry Polymers - metabolism Polysaccharide Starch and polysaccharides |
| title | Formation of polyelectrolyte complexes with diethylaminoethyl dextran: Charge ratio and molar mass effect |
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