Selective recovery of RNAs from bacterial pathogens after their internalization by human host cells

•Method for bacterial RNA recovery from host cells.•Easy and inexpensive procedure to selectively recover bacterial RNAs from composite cell mix.•Key influence on beads size and composition during extraction. Selective RNA extractions are required when studying bacterial gene expression within compl...

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2018-07, Vol.143, p.4-11
Hauptverfasser:	Raynaud, Simon, Le Pabic, Hélène, Felden, Brice
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial & prokaryotic RNAs Bacterial internalization Bacteriology Biochemistry, Molecular Biology Biological Assay - instrumentation Biological Assay - methods Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cell Line Gene Expression Regulation, Bacterial Genomics Host-pathogen interactions Host-Pathogen Interactions - genetics Humans Life Sciences Macrophages Microbiology and Parasitology Osteoblasts RNA expression RNA, Bacterial - genetics RNA, Bacterial - isolation & purification Salmonella typhimurium - genetics Selective RNA purification Staphylococcus aureus - genetics Zirconium - chemistry zirconium beads
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