Detection of minority variants within bovine respiratory syncytial virus populations using oligonucleotide-based microarrays
Microarray technology, originally developed for highly parallel examination of gene expression is regarded as a potential tool in prognosis and diagnosis. With respect to a discrimination analysis, difference as small as one nucleotide base can be distinguished using oligonucleotide-based microarray...
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| Veröffentlicht in: | Journal of virological methods 2008-03, Vol.148 (1), p.271-276 |
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| creator | Leberre, Véronique Baranowski, Eric Deplanche, Martine Trouilh, Lidwine François, Jean Marie |
| description | Microarray technology, originally developed for highly parallel examination of gene expression is regarded as a potential tool in prognosis and diagnosis. With respect to a discrimination analysis, difference as small as one nucleotide base can be distinguished using oligonucleotide-based microarrays. However, this degree of specificity is dependent on several parameters, including the size of the oligoprobes and the sequence context of the probes (
e.g. local melting temperature), hybridization conditions and to some extent the chemistry of the glass slides onto which the probes are deposited. Using bovine respiratory syncytial virus (BRSV) as a model study, an oligonucleotide-based microarray approach was developed to measure the relative abundance of a particular single nucleotide variant within mixed BRSV populations. Using this technology, we show that it is possible to discriminate at a rate of 1%, minority variants in a BRSV population. |
| doi_str_mv | 10.1016/j.jviromet.2007.10.026 |
| format | Article |
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| ispartof | Journal of virological methods, 2008-03, Vol.148 (1), p.271-276 |
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| subjects | Animals Biochemistry, Molecular Biology Biological and medical sciences Bovine respiratory syncytial virus BRSV Cell Line Cricetinae Discrimination Fundamental and applied biological sciences. Psychology Genetic Variation Genomics Humans Life Sciences Mesocricetus Microarray Analysis Microbiology Oligo-microarrays Oligonucleotide Array Sequence Analysis - methods Respiratory Syncytial Virus, Bovine - genetics Respiratory Syncytial Virus, Bovine - isolation & purification Techniques used in virology Virology |
| title | Detection of minority variants within bovine respiratory syncytial virus populations using oligonucleotide-based microarrays |
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