A method for the quantitative extraction of gold nanoparticles from human bronchoalveolar lavage fluids through a glycerol gradient
Bronchoalveolar lavage (BAL) is a diagnostic procedure which samples the cellular and non-cellular components of the pulmonary epithelial surface. The inherent biological noise of BAL fluids inhibits their direct mineralogical analysis while currently available particle retrieval protocols are suspe...
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| Veröffentlicht in: | Nanoscale 2018-01, Vol.10 (6), p.2955-2969 |
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| creator | Bitounis, Dimitrios Barnier, Vincent Guibert, Cyril Pourchez, Jérémie Forest, Valérie Boudard, Delphine Hochepied, Jean-François Chelle, Pierre Vergnon, Jean-Michel Cottier, Michèle |
| description | Bronchoalveolar lavage (BAL) is a diagnostic procedure which samples the cellular and non-cellular components of the pulmonary epithelial surface. The inherent biological noise of BAL fluids inhibits their direct mineralogical analysis while currently available particle retrieval protocols are suspected to impose quantitative and qualitative bias on the studied particle load. This study presents a simple method for the near-lossless extraction of citrate-capped gold nanoparticles from human BAL fluids at sub-ppm levels which enables their quantitation and surface characterization. This procedure was modeled according to fundamental principles of particle sedimentation and liquid-liquid interdiffusion and was evaluated by a battery of analytical techniques. The extraction yield of gold nanoparticles ranged from 61 to 86%, with a quantitation limit at 0.5 μg ml
, as measured by inductively-coupled optical emission spectroscopy. Dynamic light scattering could resolve the hydrodynamic size distribution of extracted particles which returned significantly different photon count rates at various concentrations. Their shape and primary size were easily observable by electron microscopy while atomic force microscopy, Auger electron spectroscopy and X-ray photoelectron spectroscopy could respectively probe the particles' biomolecular corona, detect surface-adsorbed S- and N- species, and identify carbon-based covalent bonds. |
| doi_str_mv | 10.1039/c7nr04484d |
| format | Article |
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, as measured by inductively-coupled optical emission spectroscopy. Dynamic light scattering could resolve the hydrodynamic size distribution of extracted particles which returned significantly different photon count rates at various concentrations. Their shape and primary size were easily observable by electron microscopy while atomic force microscopy, Auger electron spectroscopy and X-ray photoelectron spectroscopy could respectively probe the particles' biomolecular corona, detect surface-adsorbed S- and N- species, and identify carbon-based covalent bonds.</description><identifier>ISSN: 2040-3364</identifier><identifier>EISSN: 2040-3372</identifier><identifier>DOI: 10.1039/c7nr04484d</identifier><identifier>PMID: 29372197</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Atomic force microscopy ; Bioengineering ; Bronchoalveolar Lavage Fluid - chemistry ; Computational fluid dynamics ; Condensed Matter ; Covalent bonds ; Diagnostic systems ; Electron microscopy ; Fluids ; Glycerol ; Gold ; Humans ; Interdiffusion ; Life Sciences ; Materials Science ; Metal Nanoparticles ; Microscopy ; Nanoparticles ; Optical emission spectroscopy ; Particle Size ; Particle size distribution ; Photon correlation spectroscopy ; Physics ; Sedimentation ; Spectrum analysis ; Surface properties</subject><ispartof>Nanoscale, 2018-01, Vol.10 (6), p.2955-2969</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-5e5bc18401f548bbdb66ba36fbd6b1ecd07ab47cc5055bebb3a2db078f25a453</citedby><cites>FETCH-LOGICAL-c385t-5e5bc18401f548bbdb66ba36fbd6b1ecd07ab47cc5055bebb3a2db078f25a453</cites><orcidid>0000-0003-3124-0413 ; 0000-0002-9739-3300 ; 0000-0002-4573-6711 ; 0000-0002-0167-4774</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29372197$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://minesparis-psl.hal.science/hal-01806118$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Bitounis, Dimitrios</creatorcontrib><creatorcontrib>Barnier, Vincent</creatorcontrib><creatorcontrib>Guibert, Cyril</creatorcontrib><creatorcontrib>Pourchez, Jérémie</creatorcontrib><creatorcontrib>Forest, Valérie</creatorcontrib><creatorcontrib>Boudard, Delphine</creatorcontrib><creatorcontrib>Hochepied, Jean-François</creatorcontrib><creatorcontrib>Chelle, Pierre</creatorcontrib><creatorcontrib>Vergnon, Jean-Michel</creatorcontrib><creatorcontrib>Cottier, Michèle</creatorcontrib><title>A method for the quantitative extraction of gold nanoparticles from human bronchoalveolar lavage fluids through a glycerol gradient</title><title>Nanoscale</title><addtitle>Nanoscale</addtitle><description>Bronchoalveolar lavage (BAL) is a diagnostic procedure which samples the cellular and non-cellular components of the pulmonary epithelial surface. The inherent biological noise of BAL fluids inhibits their direct mineralogical analysis while currently available particle retrieval protocols are suspected to impose quantitative and qualitative bias on the studied particle load. This study presents a simple method for the near-lossless extraction of citrate-capped gold nanoparticles from human BAL fluids at sub-ppm levels which enables their quantitation and surface characterization. This procedure was modeled according to fundamental principles of particle sedimentation and liquid-liquid interdiffusion and was evaluated by a battery of analytical techniques. The extraction yield of gold nanoparticles ranged from 61 to 86%, with a quantitation limit at 0.5 μg ml
, as measured by inductively-coupled optical emission spectroscopy. Dynamic light scattering could resolve the hydrodynamic size distribution of extracted particles which returned significantly different photon count rates at various concentrations. Their shape and primary size were easily observable by electron microscopy while atomic force microscopy, Auger electron spectroscopy and X-ray photoelectron spectroscopy could respectively probe the particles' biomolecular corona, detect surface-adsorbed S- and N- species, and identify carbon-based covalent bonds.</description><subject>Atomic force microscopy</subject><subject>Bioengineering</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Computational fluid dynamics</subject><subject>Condensed Matter</subject><subject>Covalent bonds</subject><subject>Diagnostic systems</subject><subject>Electron microscopy</subject><subject>Fluids</subject><subject>Glycerol</subject><subject>Gold</subject><subject>Humans</subject><subject>Interdiffusion</subject><subject>Life Sciences</subject><subject>Materials Science</subject><subject>Metal Nanoparticles</subject><subject>Microscopy</subject><subject>Nanoparticles</subject><subject>Optical emission spectroscopy</subject><subject>Particle Size</subject><subject>Particle size distribution</subject><subject>Photon correlation spectroscopy</subject><subject>Physics</subject><subject>Sedimentation</subject><subject>Spectrum analysis</subject><subject>Surface properties</subject><issn>2040-3364</issn><issn>2040-3372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0ctq3DAUBmBTGppLu-kDFEE3bWFaybrYXg7TpgkMLZTszZF0fAmyNJHkIVn3xeN00llkpYP4-DmHvyjeM_qVUd58M5WPVIha2FfFWUkFXXFela-PsxKnxXlKt5Sqhiv-pjgtmwWwpjor_q7JhHkIlnQhkjwguZvB5zFDHvdI8D5HMHkMnoSO9MFZ4sGHHcQ8GoeJdDFMZJgn8ETH4M0QwO0xOIjEwR56JJ2bR5uW6BjmfiBAevdgMAZH-gh2RJ_fFicduITvnt-L4ubyx83marX9_fN6s96uDK9lXkmU2rBaUNZJUWtttVIauOq0VZqhsbQCLSpjJJVSo9YcSqtpVXelBCH5RfH5EDuAa3dxnCA-tAHG9mq9bZ_-KKupYqzes8V-OthdDHczptxOYzLoHHgMc2pZ05SUlZw1C_34gt6GOfrlkHYhtFaN4nxRXw7KxJBSxO64AaPtU4vtpvr151-L3xf84Tly1hPaI_1fG38EA2mZxQ</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Bitounis, Dimitrios</creator><creator>Barnier, Vincent</creator><creator>Guibert, Cyril</creator><creator>Pourchez, Jérémie</creator><creator>Forest, Valérie</creator><creator>Boudard, Delphine</creator><creator>Hochepied, Jean-François</creator><creator>Chelle, Pierre</creator><creator>Vergnon, Jean-Michel</creator><creator>Cottier, Michèle</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0003-3124-0413</orcidid><orcidid>https://orcid.org/0000-0002-9739-3300</orcidid><orcidid>https://orcid.org/0000-0002-4573-6711</orcidid><orcidid>https://orcid.org/0000-0002-0167-4774</orcidid></search><sort><creationdate>20180101</creationdate><title>A method for the quantitative extraction of gold nanoparticles from human bronchoalveolar lavage fluids through a glycerol gradient</title><author>Bitounis, Dimitrios ; 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The inherent biological noise of BAL fluids inhibits their direct mineralogical analysis while currently available particle retrieval protocols are suspected to impose quantitative and qualitative bias on the studied particle load. This study presents a simple method for the near-lossless extraction of citrate-capped gold nanoparticles from human BAL fluids at sub-ppm levels which enables their quantitation and surface characterization. This procedure was modeled according to fundamental principles of particle sedimentation and liquid-liquid interdiffusion and was evaluated by a battery of analytical techniques. The extraction yield of gold nanoparticles ranged from 61 to 86%, with a quantitation limit at 0.5 μg ml
, as measured by inductively-coupled optical emission spectroscopy. Dynamic light scattering could resolve the hydrodynamic size distribution of extracted particles which returned significantly different photon count rates at various concentrations. Their shape and primary size were easily observable by electron microscopy while atomic force microscopy, Auger electron spectroscopy and X-ray photoelectron spectroscopy could respectively probe the particles' biomolecular corona, detect surface-adsorbed S- and N- species, and identify carbon-based covalent bonds.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>29372197</pmid><doi>10.1039/c7nr04484d</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-3124-0413</orcidid><orcidid>https://orcid.org/0000-0002-9739-3300</orcidid><orcidid>https://orcid.org/0000-0002-4573-6711</orcidid><orcidid>https://orcid.org/0000-0002-0167-4774</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008- |
| subjects | Atomic force microscopy Bioengineering Bronchoalveolar Lavage Fluid - chemistry Computational fluid dynamics Condensed Matter Covalent bonds Diagnostic systems Electron microscopy Fluids Glycerol Gold Humans Interdiffusion Life Sciences Materials Science Metal Nanoparticles Microscopy Nanoparticles Optical emission spectroscopy Particle Size Particle size distribution Photon correlation spectroscopy Physics Sedimentation Spectrum analysis Surface properties |
| title | A method for the quantitative extraction of gold nanoparticles from human bronchoalveolar lavage fluids through a glycerol gradient |
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