Ultra-sensitive biosensor based on genetically engineered acetylcholinesterase immobilized in poly (vinyl alcohol)/Fe–Ni alloy nanocomposite for phosmet detection in olive oil

•Acetylcholinesterase was intrapped in Fe/Ni nanopowder modified photopolymer.•Fe/Ni nanocomposite allows ampifying the current and lowering the applied potential.•The biosensor allowed highly sensitive detection of phosmet, the LOD was 0.1nM.•Recovery rate of phosmet was 96% after liquid–liquid ext...

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Veröffentlicht in:	Food chemistry 2016-07, Vol.203, p.73-78
Hauptverfasser:	El-Moghazy, A.Y., Soliman, E.A., Ibrahim, H.Z., Noguer, T., Marty, J.-L., Istamboulie, G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcholinesterase Acetylcholinesterase - chemistry Acetylcholinesterase - genetics Alloys - chemistry Analytical chemistry Biosensing Techniques - methods Biosensor Chemical Sciences Enzymes, Immobilized - chemistry Enzymes, Immobilized - genetics Iron - chemistry Limit of Detection Liquid-Liquid Extraction Nanocomposite Nanocomposites - chemistry Nickel - chemistry Olea Olive oil Olive Oil - analysis Organophosphorus pesticides Phosmet - analysis Polyvinyl Alcohol - chemistry Reproducibility of Results Screen-printed electrodes
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