Influence of Adsorption on Proteins and Amyloid Detection by Silicon Nitride Nanopore

For the past 2 decades, emerging single-nanopore technologies have opened the route to multiple sensing applications. Besides DNA sensing, the identification of proteins and amyloids is a promising field for early diagnosis. However, the influence of the interactions between the nanopore surface and...

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Veröffentlicht in:Langmuir 2016-09, Vol.32 (35), p.8916-8925
Hauptverfasser: Balme, Sébastien, Coulon, Pierre Eugène, Lepoitevin, Mathilde, Charlot, Benoît, Yandrapalli, Naresh, Favard, Cyril, Muriaux, Delphine, Bechelany, Mikhael, Janot, Jean-Marc
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container_end_page 8925
container_issue 35
container_start_page 8916
container_title Langmuir
container_volume 32
creator Balme, Sébastien
Coulon, Pierre Eugène
Lepoitevin, Mathilde
Charlot, Benoît
Yandrapalli, Naresh
Favard, Cyril
Muriaux, Delphine
Bechelany, Mikhael
Janot, Jean-Marc
description For the past 2 decades, emerging single-nanopore technologies have opened the route to multiple sensing applications. Besides DNA sensing, the identification of proteins and amyloids is a promising field for early diagnosis. However, the influence of the interactions between the nanopore surface and proteins should be taken into account. In this work, we have selected three proteins (avidin, lysozyme, and IgG) that exhibit different affinities with the SiN x surface, and we have also examined lysozyme amyloid. Our results show that the piranha treatment of SiN x significantly decreases protein adsorption. Moreover, we have successfully detected all proteins (pore diameter 17 nm) and shown the possibility of discriminating between denatured lysozyme and its amyloid. For all proteins, the capture rates are lower than expected, and we evidence that they are correlated with the affinity of proteins to the surface. Our result confirms that proteins interacting only with the nanopore surface wall stay long enough to be detected. For lysozyme amyloid, we show that the use of the nanopore is suitable for determining the number of monomer units even if only the proteins interacting with the nanopore are detected.
doi_str_mv 10.1021/acs.langmuir.6b02048
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source MEDLINE; American Chemical Society Journals
subjects Adsorption
Amyloid - analysis
Amyloid - chemistry
Avidin - analysis
Avidin - chemistry
Chemical Sciences
Electrochemical Techniques
Immunoglobulin G - analysis
Immunoglobulin G - chemistry
Interface Components: Nanops, Colloids, Emulsions, Surfactants, Proteins, Polymers
Kinetics
Muramidase - analysis
Muramidase - chemistry
Nanopores - ultrastructure
Silicon Compounds - chemistry
Solutions
title Influence of Adsorption on Proteins and Amyloid Detection by Silicon Nitride Nanopore
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