Spectral imaging detection and counting of microbial cells in marine sediment

Semiautomated detection and counting techniques for microbial cells in soil and marine sediment using microscopic-spectral-imaging analysis were developed. Microbial cells in microscopic fields were selectively detected from other fluorescent particles by their fluorescent spectrum, based on the spe...

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Veröffentlicht in:	Journal of microbiological methods 2003-04, Vol.53 (1), p.57-65
Hauptverfasser:	Sunamura, Michinari, Maruyama, Akihiko, Tsuji, Takashi, Kurane, Ryuichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria - classification Bacteria - growth & development Bacteria - isolation & purification Biological and medical sciences Colony Count, Microbial - methods Direct counting Earth Sciences Fluorescent Dyes - chemistry Fluorescent microscope Fundamental and applied biological sciences. Psychology Geologic Sediments - microbiology Image analysis Indoles - chemistry Marine Microbiology Microscopy, Fluorescence Oceanography Sciences of the Universe Seawater - microbiology Spectral imaging Spectrometry, Fluorescence - methods SYBR Green II Water Microbiology
Online-Zugang:	Volltext
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