Detection of long-range electrostatic interactions between charged molecules by means of fluorescence correlation spectroscopy
In the present paper, an experimental feasibility study on the detection of long-range intermolecular interactions through three-dimensional molecular diffusion in solution is performed. This follows recent theoretical and numerical analyses reporting that long-range electrodynamic forces between bi...
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| Veröffentlicht in: | Physical review. E 2017-08, Vol.96 (2-1), p.022403-022403, Article 022403 |
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| container_title | Physical review. E |
| container_volume | 96 |
| creator | Nardecchia, Ilaria Lechelon, Mathias Gori, Matteo Donato, Irene Preto, Jordane Floriani, Elena Jaeger, Sebastien Mailfert, Sebastien Marguet, Didier Ferrier, Pierre Pettini, Marco |
| description | In the present paper, an experimental feasibility study on the detection of long-range intermolecular interactions through three-dimensional molecular diffusion in solution is performed. This follows recent theoretical and numerical analyses reporting that long-range electrodynamic forces between biomolecules could be identified through deviations from Brownian diffusion. The suggested experimental technique was fluorescence correlation spectroscopy (FCS). By considering two oppositely charged molecular species in aqueous solution, namely, lysozymes and fluorescent dye molecules (Alexa488), the diffusion coefficient of the dyes has been measured for different values of the concentration of lysozyme, that is, for different average distances between the oppositely charged molecules. For our model, long-range interactions are of electrostatic origin, suggesting that their action radius can be varied by changing the ionic strength of the solution. The experimental outcomes clearly prove the detectability of long-range intermolecular interactions by means of the FCS technique. Molecular dynamics simulations provide a clear and unambiguous interpretation of the experimental results. |
| doi_str_mv | 10.1103/PhysRevE.96.022403 |
| format | Article |
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This follows recent theoretical and numerical analyses reporting that long-range electrodynamic forces between biomolecules could be identified through deviations from Brownian diffusion. The suggested experimental technique was fluorescence correlation spectroscopy (FCS). By considering two oppositely charged molecular species in aqueous solution, namely, lysozymes and fluorescent dye molecules (Alexa488), the diffusion coefficient of the dyes has been measured for different values of the concentration of lysozyme, that is, for different average distances between the oppositely charged molecules. For our model, long-range interactions are of electrostatic origin, suggesting that their action radius can be varied by changing the ionic strength of the solution. The experimental outcomes clearly prove the detectability of long-range intermolecular interactions by means of the FCS technique. Molecular dynamics simulations provide a clear and unambiguous interpretation of the experimental results.</description><identifier>ISSN: 2470-0045</identifier><identifier>EISSN: 2470-0053</identifier><identifier>DOI: 10.1103/PhysRevE.96.022403</identifier><identifier>PMID: 28950524</identifier><language>eng</language><publisher>United States: American Physical Society (APS)</publisher><subject>Algorithms ; Animals ; Biological Physics ; Chickens ; Diffusion ; Egg Proteins - chemistry ; Egg Proteins - metabolism ; Equipment Design ; Fluorescent Dyes - chemistry ; Fluorobenzenes - chemistry ; Ions - chemistry ; Microscopy, Fluorescence ; Molecular Dynamics Simulation ; Muramidase - chemistry ; Muramidase - metabolism ; Physics ; Solutions ; Spectrometry, Fluorescence - instrumentation ; Spectrometry, Fluorescence - methods ; Static Electricity ; Water - chemistry</subject><ispartof>Physical review. 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E</title><addtitle>Phys Rev E</addtitle><description>In the present paper, an experimental feasibility study on the detection of long-range intermolecular interactions through three-dimensional molecular diffusion in solution is performed. This follows recent theoretical and numerical analyses reporting that long-range electrodynamic forces between biomolecules could be identified through deviations from Brownian diffusion. The suggested experimental technique was fluorescence correlation spectroscopy (FCS). By considering two oppositely charged molecular species in aqueous solution, namely, lysozymes and fluorescent dye molecules (Alexa488), the diffusion coefficient of the dyes has been measured for different values of the concentration of lysozyme, that is, for different average distances between the oppositely charged molecules. For our model, long-range interactions are of electrostatic origin, suggesting that their action radius can be varied by changing the ionic strength of the solution. The experimental outcomes clearly prove the detectability of long-range intermolecular interactions by means of the FCS technique. Molecular dynamics simulations provide a clear and unambiguous interpretation of the experimental results.</description><subject>Algorithms</subject><subject>Animals</subject><subject>Biological Physics</subject><subject>Chickens</subject><subject>Diffusion</subject><subject>Egg Proteins - chemistry</subject><subject>Egg Proteins - metabolism</subject><subject>Equipment Design</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorobenzenes - chemistry</subject><subject>Ions - chemistry</subject><subject>Microscopy, Fluorescence</subject><subject>Molecular Dynamics Simulation</subject><subject>Muramidase - chemistry</subject><subject>Muramidase - metabolism</subject><subject>Physics</subject><subject>Solutions</subject><subject>Spectrometry, Fluorescence - instrumentation</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Static Electricity</subject><subject>Water - chemistry</subject><issn>2470-0045</issn><issn>2470-0053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kcFO3DAQhq2qCBDwAhwqH-khi-3EcXxEQEullUCoPVsTe7Kbyom3dkK1lz57DYE92Rp_843GPyGXnK04Z-X103afnvHlfqXrFROiYuUncioqxQrGZPn5cK_kCblI6TdjjNdMKy6OyYlotGRSVKfk3x1OaKc-jDR01IdxU0QYN0jR53IMaYKpt7QfJ4zwxiXa4vQXcaR2C3GDjg4hs7PH_LKnA0JGsqvzc4iYLI4WqQ0xooe3OWm3mG3Y7c_JUQc-4cX7eUZ-fbv_eftQrB-__7i9WRe2bPhUOOxaUNBp1UJtmXPSKSYrYcHq1oEW0LWNAqss8lJqdBycQKuEEK1UoMoz8nXxbsGbXewHiHsToDcPN2vzWsufU4um0S88s1cLu4vhz4xpMkOf1_AeRgxzMlxXZV3VUlUZFQtq8z4pYndwc2ZeYzIfMRldmyWm3PTl3T-3A7pDy0co5X-COpO8</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>Nardecchia, Ilaria</creator><creator>Lechelon, Mathias</creator><creator>Gori, Matteo</creator><creator>Donato, Irene</creator><creator>Preto, Jordane</creator><creator>Floriani, Elena</creator><creator>Jaeger, Sebastien</creator><creator>Mailfert, Sebastien</creator><creator>Marguet, Didier</creator><creator>Ferrier, Pierre</creator><creator>Pettini, Marco</creator><general>American Physical Society (APS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-0409-0432</orcidid><orcidid>https://orcid.org/0000-0003-1561-4390</orcidid></search><sort><creationdate>201708</creationdate><title>Detection of long-range electrostatic interactions between charged molecules by means of fluorescence correlation spectroscopy</title><author>Nardecchia, Ilaria ; Lechelon, Mathias ; Gori, Matteo ; Donato, Irene ; Preto, Jordane ; Floriani, Elena ; Jaeger, Sebastien ; Mailfert, Sebastien ; Marguet, Didier ; Ferrier, Pierre ; Pettini, Marco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-defba7af97ba6c0dd5d70542cac9bda92afb87ac7ce1359ed1ad2ec7222b57a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Algorithms</topic><topic>Animals</topic><topic>Biological Physics</topic><topic>Chickens</topic><topic>Diffusion</topic><topic>Egg Proteins - chemistry</topic><topic>Egg Proteins - metabolism</topic><topic>Equipment Design</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorobenzenes - chemistry</topic><topic>Ions - chemistry</topic><topic>Microscopy, Fluorescence</topic><topic>Molecular Dynamics Simulation</topic><topic>Muramidase - chemistry</topic><topic>Muramidase - metabolism</topic><topic>Physics</topic><topic>Solutions</topic><topic>Spectrometry, Fluorescence - instrumentation</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Static Electricity</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nardecchia, Ilaria</creatorcontrib><creatorcontrib>Lechelon, Mathias</creatorcontrib><creatorcontrib>Gori, Matteo</creatorcontrib><creatorcontrib>Donato, Irene</creatorcontrib><creatorcontrib>Preto, Jordane</creatorcontrib><creatorcontrib>Floriani, Elena</creatorcontrib><creatorcontrib>Jaeger, Sebastien</creatorcontrib><creatorcontrib>Mailfert, Sebastien</creatorcontrib><creatorcontrib>Marguet, Didier</creatorcontrib><creatorcontrib>Ferrier, Pierre</creatorcontrib><creatorcontrib>Pettini, Marco</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Physical review. 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By considering two oppositely charged molecular species in aqueous solution, namely, lysozymes and fluorescent dye molecules (Alexa488), the diffusion coefficient of the dyes has been measured for different values of the concentration of lysozyme, that is, for different average distances between the oppositely charged molecules. For our model, long-range interactions are of electrostatic origin, suggesting that their action radius can be varied by changing the ionic strength of the solution. The experimental outcomes clearly prove the detectability of long-range intermolecular interactions by means of the FCS technique. 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| subjects | Algorithms Animals Biological Physics Chickens Diffusion Egg Proteins - chemistry Egg Proteins - metabolism Equipment Design Fluorescent Dyes - chemistry Fluorobenzenes - chemistry Ions - chemistry Microscopy, Fluorescence Molecular Dynamics Simulation Muramidase - chemistry Muramidase - metabolism Physics Solutions Spectrometry, Fluorescence - instrumentation Spectrometry, Fluorescence - methods Static Electricity Water - chemistry |
| title | Detection of long-range electrostatic interactions between charged molecules by means of fluorescence correlation spectroscopy |
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