Separation and fractionation of exopolysaccharides from Porphyridium cruentum
In this work the extraction of EPSs from culture media of Porphyridium cruentum, by dialysis, solvent-precipitation with 3 polar alcohols (methanol, ethanol and isopropanol) and membrane separation techniques has been studied. Diafiltration (DF) using a membrane with a 300kDa molecular weight cut of...
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| Veröffentlicht in: | Bioresource technology 2013-10, Vol.145, p.345-350 |
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| creator | Patel, Anil Kumar Laroche, Celine Marcati, Alain Ursu, Alina Violeta Jubeau, Sébastien Marchal, Luc Petit, Emmanuel Djelveh, Gholamreza Michaud, Philippe |
| description | In this work the extraction of EPSs from culture media of Porphyridium cruentum, by dialysis, solvent-precipitation with 3 polar alcohols (methanol, ethanol and isopropanol) and membrane separation techniques has been studied. Diafiltration (DF) using a membrane with a 300kDa molecular weight cut off was the most efficient technique compared to solvent-extraction and dialysis methods. After extraction, EPS fraction was characterized in terms of rheological properties and biochemical content. The product exhibited shear thinning behavior and a critical overlap concentration equal to 0.6g/L. The monosaccharide composition was investigated after acidic hydrolysis. Xylose, galactose, glucose and glucuronic acid were identified as the main constitutive monomers. |
| doi_str_mv | 10.1016/j.biortech.2012.12.038 |
| format | Article |
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Diafiltration (DF) using a membrane with a 300kDa molecular weight cut off was the most efficient technique compared to solvent-extraction and dialysis methods. After extraction, EPS fraction was characterized in terms of rheological properties and biochemical content. The product exhibited shear thinning behavior and a critical overlap concentration equal to 0.6g/L. The monosaccharide composition was investigated after acidic hydrolysis. Xylose, galactose, glucose and glucuronic acid were identified as the main constitutive monomers.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2012.12.038</identifier><identifier>PMID: 23313179</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>2-Propanol ; Biochemistry ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Biotechnology ; Chemical and Process Engineering ; Chemical Precipitation ; Concentration (composition) ; Culture Media - chemistry ; Diafiltration ; Dialysis ; Dialysis - methods ; Engineering Sciences ; Ethanol ; Ethyl alcohol ; Exopolysaccharide ; Extraction ; Filtration - methods ; Fundamental and applied biological sciences. Psychology ; Hydrolysis ; Life Sciences ; Membranes ; Methanol ; Methyl alcohol ; Monosaccharides - analysis ; Polysaccharides, Bacterial - chemistry ; Polysaccharides, Bacterial - isolation & purification ; Porphyridium - chemistry ; Porphyridium cruentum ; Porphyridium sp ; Rheology ; Separation</subject><ispartof>Bioresource technology, 2013-10, Vol.145, p.345-350</ispartof><rights>2012 Elsevier Ltd</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ltd. 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Diafiltration (DF) using a membrane with a 300kDa molecular weight cut off was the most efficient technique compared to solvent-extraction and dialysis methods. After extraction, EPS fraction was characterized in terms of rheological properties and biochemical content. The product exhibited shear thinning behavior and a critical overlap concentration equal to 0.6g/L. The monosaccharide composition was investigated after acidic hydrolysis. Xylose, galactose, glucose and glucuronic acid were identified as the main constitutive monomers.</description><subject>2-Propanol</subject><subject>Biochemistry</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chemical and Process Engineering</subject><subject>Chemical Precipitation</subject><subject>Concentration (composition)</subject><subject>Culture Media - chemistry</subject><subject>Diafiltration</subject><subject>Dialysis</subject><subject>Dialysis - methods</subject><subject>Engineering Sciences</subject><subject>Ethanol</subject><subject>Ethyl alcohol</subject><subject>Exopolysaccharide</subject><subject>Extraction</subject><subject>Filtration - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolysis</subject><subject>Life Sciences</subject><subject>Membranes</subject><subject>Methanol</subject><subject>Methyl alcohol</subject><subject>Monosaccharides - analysis</subject><subject>Polysaccharides, Bacterial - chemistry</subject><subject>Polysaccharides, Bacterial - isolation & purification</subject><subject>Porphyridium - chemistry</subject><subject>Porphyridium cruentum</subject><subject>Porphyridium sp</subject><subject>Rheology</subject><subject>Separation</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9r3DAQxUVpaTZJv0LwpdAcvNUfy5JuDaFJCltaaHMWsjxitdiWK9mh--0r4016DAxIevxm9JiH0BXBW4JJ_fmwbXyIE9j9lmJCt7kwk2_QhkjBSqpE_RZtsKpxKTmtztB5SgeMMSOCvkdnlDGSr2qDvv-C0UQz-TAUZmgLF41dHqsSXAF_wxi6YzLW7k30LaTMhL74GeK4P2bBz31h4wzDNPeX6J0zXYIPp_MCPd59_X37UO5-3H-7vdmVtqr5VDpgrmHKElwp5YRwTSNwowCMrFvWGFpZybGSsuW1UyAxY8xUDnOpKKcE2AW6XufuTafH6HsTjzoYrx9udnrR8oq4FII9kcx-Wtkxhj8zpEn3PlnoOjNAmJMmHGMhCOfV62hFlayynWVqvaI2hpQiuBcbBOslIX3QzwnpJSGdKyeUG69Of8xND-1L23MkGfh4AkyypsuBDNan_5wQNa0wz9yXlYO86CcPUSfrYbDQ-gh20m3wr3n5B8dBsdI</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Patel, Anil Kumar</creator><creator>Laroche, Celine</creator><creator>Marcati, Alain</creator><creator>Ursu, Alina Violeta</creator><creator>Jubeau, Sébastien</creator><creator>Marchal, Luc</creator><creator>Petit, Emmanuel</creator><creator>Djelveh, Gholamreza</creator><creator>Michaud, Philippe</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SU</scope><scope>7TB</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-2705-2866</orcidid><orcidid>https://orcid.org/0000-0003-1533-6909</orcidid><orcidid>https://orcid.org/0000-0003-3828-1867</orcidid><orcidid>https://orcid.org/0000-0002-6677-4549</orcidid><orcidid>https://orcid.org/0000-0002-9915-0126</orcidid></search><sort><creationdate>20131001</creationdate><title>Separation and fractionation of exopolysaccharides from Porphyridium cruentum</title><author>Patel, Anil Kumar ; Laroche, Celine ; Marcati, Alain ; Ursu, Alina Violeta ; Jubeau, Sébastien ; Marchal, Luc ; Petit, Emmanuel ; Djelveh, Gholamreza ; Michaud, Philippe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-fe3fb39c10499f77fbb70b9eea86d3ba24c850988d56f9e80333a4f05892521e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>2-Propanol</topic><topic>Biochemistry</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chemical and Process Engineering</topic><topic>Chemical Precipitation</topic><topic>Concentration (composition)</topic><topic>Culture Media - chemistry</topic><topic>Diafiltration</topic><topic>Dialysis</topic><topic>Dialysis - methods</topic><topic>Engineering Sciences</topic><topic>Ethanol</topic><topic>Ethyl alcohol</topic><topic>Exopolysaccharide</topic><topic>Extraction</topic><topic>Filtration - methods</topic><topic>Fundamental and applied biological sciences. 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Diafiltration (DF) using a membrane with a 300kDa molecular weight cut off was the most efficient technique compared to solvent-extraction and dialysis methods. After extraction, EPS fraction was characterized in terms of rheological properties and biochemical content. The product exhibited shear thinning behavior and a critical overlap concentration equal to 0.6g/L. The monosaccharide composition was investigated after acidic hydrolysis. Xylose, galactose, glucose and glucuronic acid were identified as the main constitutive monomers.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>23313179</pmid><doi>10.1016/j.biortech.2012.12.038</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-2705-2866</orcidid><orcidid>https://orcid.org/0000-0003-1533-6909</orcidid><orcidid>https://orcid.org/0000-0003-3828-1867</orcidid><orcidid>https://orcid.org/0000-0002-6677-4549</orcidid><orcidid>https://orcid.org/0000-0002-9915-0126</orcidid></addata></record> |
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| ispartof | Bioresource technology, 2013-10, Vol.145, p.345-350 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 2-Propanol Biochemistry Biochemistry, Molecular Biology Biological and medical sciences Biotechnology Chemical and Process Engineering Chemical Precipitation Concentration (composition) Culture Media - chemistry Diafiltration Dialysis Dialysis - methods Engineering Sciences Ethanol Ethyl alcohol Exopolysaccharide Extraction Filtration - methods Fundamental and applied biological sciences. Psychology Hydrolysis Life Sciences Membranes Methanol Methyl alcohol Monosaccharides - analysis Polysaccharides, Bacterial - chemistry Polysaccharides, Bacterial - isolation & purification Porphyridium - chemistry Porphyridium cruentum Porphyridium sp Rheology Separation |
| title | Separation and fractionation of exopolysaccharides from Porphyridium cruentum |
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