Toward the Standardization of Mycological Examination of Sputum Samples in Cystic Fibrosis: Results from a French Multicenter Prospective Study
Fungal respiratory colonization of cystic fibrosis (CF) patients emerges as a new concern; however, the heterogeneity of mycological protocols limits investigations. We first aimed at setting up an efficient standardized protocol for mycological analysis of CF sputa that was assessed during a prospe...
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| Veröffentlicht in: | Mycopathologia (1975) 2018-02, Vol.183 (1), p.101-117 |
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| creator | Coron, Noémie Pihet, Marc Fréalle, Emilie Lemeille, Yolande Pinel, Claudine Pelloux, Hervé Gargala, Gilles Favennec, Loic Accoceberry, Isabelle Durand-Joly, Isabelle Dalle, Frédéric Huet, Frédéric Fanton, Annlyse Boldron, Amale Loeuille, Guy-André Domblides, Philippe Coltey, Bérengère Pin, Isabelle Llerena, Catherine Troussier, Françoise Person, Christine Marguet, Christophe Wizla, Nathalie Thumerelle, Caroline Turck, Dominique Bui, Stéphanie Fayon, Michael Duhamel, Alain Prévotat, Anne Wallaert, Benoit Leroy, Sylvie Bouchara, Jean-Philippe Delhaes, Laurence |
| description | Fungal respiratory colonization of cystic fibrosis (CF) patients emerges as a new concern; however, the heterogeneity of mycological protocols limits investigations. We first aimed at setting up an efficient standardized protocol for mycological analysis of CF sputa that was assessed during a prospective, multicenter study: “MucoFong” program (PHRC-06/1902). Sputa from 243 CF patients from seven centers in France were collected over a 15-month period and submitted to a standardized protocol based on 6 semi-selective media. After mucolytic pretreatment, sputa were plated in parallel on cycloheximide-enriched (ACT37), erythritol-enriched (ERY37), benomyl dichloran–rose bengal (BENO37) and chromogenic (CAN37) media incubated at 37 °C and on Sabouraud–chloramphenicol (SAB27) and erythritol-enriched (ERY27) media incubated at 20–27 °C. Each plate was checked twice a week during 3 weeks. Fungi were conventionally identified; time for detection of fungal growth was noted for each species. Fungal prevalences and media performances were assessed; an optimal combination of media was determined using the Chi-squared automatic interaction detector method. At least one fungal species was isolated from 81% of sputa.
Candida albicans
was the most prevalent species (58.8%), followed by
Aspergillus fumigatus
(35.4%). Cultivation on CAN37, SAB27, ACT37 and ERY27 during 16 days provided an optimal combination, detecting
C. albicans
,
A. fumigatus
,
Scedosporium apiospermum
complex and
Exophiala
spp. with sensitivities of 96.5, 98.8, 100 and 100%. Combination of these four culture media is recommended to ensure the growth of key fungal pathogens in CF respiratory specimens. The use of such consensual protocol is of major interest for merging results from future epidemiological studies. |
| doi_str_mv | 10.1007/s11046-017-0173-1 |
| format | Article |
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Candida albicans
was the most prevalent species (58.8%), followed by
Aspergillus fumigatus
(35.4%). Cultivation on CAN37, SAB27, ACT37 and ERY27 during 16 days provided an optimal combination, detecting
C. albicans
,
A. fumigatus
,
Scedosporium apiospermum
complex and
Exophiala
spp. with sensitivities of 96.5, 98.8, 100 and 100%. Combination of these four culture media is recommended to ensure the growth of key fungal pathogens in CF respiratory specimens. The use of such consensual protocol is of major interest for merging results from future epidemiological studies.</description><identifier>ISSN: 0301-486X</identifier><identifier>EISSN: 1573-0832</identifier><identifier>DOI: 10.1007/s11046-017-0173-1</identifier><identifier>PMID: 28748285</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Analysis ; Antibacterial agents ; Benomyl ; Biomedical and Life Sciences ; Candida albicans ; Chloramphenicol ; Colonization ; Culture media ; Cycloheximide ; Cystic fibrosis ; Enrichment ; Epidemiology ; Erythritol ; Eukaryotic Microbiology ; Fibrosis ; Fungi ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbiology ; Microbiology and Parasitology ; Mycology ; Plant Sciences ; Selective media ; Species ; Sputum ; Standardization</subject><ispartof>Mycopathologia (1975), 2018-02, Vol.183 (1), p.101-117</ispartof><rights>Springer Science+Business Media B.V. 2017</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Mycopathologia is a copyright of Springer, (2017). All Rights Reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-359d582354fcd0cce48ff287dd5bfd25685429a990f0a15fbb1e27c6a6df31113</citedby><cites>FETCH-LOGICAL-c573t-359d582354fcd0cce48ff287dd5bfd25685429a990f0a15fbb1e27c6a6df31113</cites><orcidid>0000-0002-7529-6999 ; 0000-0003-3129-979X ; 0000-0002-3365-2035 ; 0000-0001-8720-5823</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11046-017-0173-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11046-017-0173-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28748285$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://u-bourgogne.hal.science/hal-01618715$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Coron, Noémie</creatorcontrib><creatorcontrib>Pihet, Marc</creatorcontrib><creatorcontrib>Fréalle, Emilie</creatorcontrib><creatorcontrib>Lemeille, Yolande</creatorcontrib><creatorcontrib>Pinel, Claudine</creatorcontrib><creatorcontrib>Pelloux, Hervé</creatorcontrib><creatorcontrib>Gargala, Gilles</creatorcontrib><creatorcontrib>Favennec, Loic</creatorcontrib><creatorcontrib>Accoceberry, Isabelle</creatorcontrib><creatorcontrib>Durand-Joly, Isabelle</creatorcontrib><creatorcontrib>Dalle, Frédéric</creatorcontrib><creatorcontrib>Huet, Frédéric</creatorcontrib><creatorcontrib>Fanton, Annlyse</creatorcontrib><creatorcontrib>Boldron, Amale</creatorcontrib><creatorcontrib>Loeuille, Guy-André</creatorcontrib><creatorcontrib>Domblides, Philippe</creatorcontrib><creatorcontrib>Coltey, Bérengère</creatorcontrib><creatorcontrib>Pin, Isabelle</creatorcontrib><creatorcontrib>Llerena, Catherine</creatorcontrib><creatorcontrib>Troussier, Françoise</creatorcontrib><creatorcontrib>Person, Christine</creatorcontrib><creatorcontrib>Marguet, Christophe</creatorcontrib><creatorcontrib>Wizla, Nathalie</creatorcontrib><creatorcontrib>Thumerelle, Caroline</creatorcontrib><creatorcontrib>Turck, Dominique</creatorcontrib><creatorcontrib>Bui, Stéphanie</creatorcontrib><creatorcontrib>Fayon, Michael</creatorcontrib><creatorcontrib>Duhamel, Alain</creatorcontrib><creatorcontrib>Prévotat, Anne</creatorcontrib><creatorcontrib>Wallaert, Benoit</creatorcontrib><creatorcontrib>Leroy, Sylvie</creatorcontrib><creatorcontrib>Bouchara, Jean-Philippe</creatorcontrib><creatorcontrib>Delhaes, Laurence</creatorcontrib><title>Toward the Standardization of Mycological Examination of Sputum Samples in Cystic Fibrosis: Results from a French Multicenter Prospective Study</title><title>Mycopathologia (1975)</title><addtitle>Mycopathologia</addtitle><addtitle>Mycopathologia</addtitle><description>Fungal respiratory colonization of cystic fibrosis (CF) patients emerges as a new concern; however, the heterogeneity of mycological protocols limits investigations. We first aimed at setting up an efficient standardized protocol for mycological analysis of CF sputa that was assessed during a prospective, multicenter study: “MucoFong” program (PHRC-06/1902). Sputa from 243 CF patients from seven centers in France were collected over a 15-month period and submitted to a standardized protocol based on 6 semi-selective media. After mucolytic pretreatment, sputa were plated in parallel on cycloheximide-enriched (ACT37), erythritol-enriched (ERY37), benomyl dichloran–rose bengal (BENO37) and chromogenic (CAN37) media incubated at 37 °C and on Sabouraud–chloramphenicol (SAB27) and erythritol-enriched (ERY27) media incubated at 20–27 °C. Each plate was checked twice a week during 3 weeks. Fungi were conventionally identified; time for detection of fungal growth was noted for each species. Fungal prevalences and media performances were assessed; an optimal combination of media was determined using the Chi-squared automatic interaction detector method. At least one fungal species was isolated from 81% of sputa.
Candida albicans
was the most prevalent species (58.8%), followed by
Aspergillus fumigatus
(35.4%). Cultivation on CAN37, SAB27, ACT37 and ERY27 during 16 days provided an optimal combination, detecting
C. albicans
,
A. fumigatus
,
Scedosporium apiospermum
complex and
Exophiala
spp. with sensitivities of 96.5, 98.8, 100 and 100%. Combination of these four culture media is recommended to ensure the growth of key fungal pathogens in CF respiratory specimens. The use of such consensual protocol is of major interest for merging results from future epidemiological studies.</description><subject>Analysis</subject><subject>Antibacterial agents</subject><subject>Benomyl</subject><subject>Biomedical and Life Sciences</subject><subject>Candida albicans</subject><subject>Chloramphenicol</subject><subject>Colonization</subject><subject>Culture media</subject><subject>Cycloheximide</subject><subject>Cystic fibrosis</subject><subject>Enrichment</subject><subject>Epidemiology</subject><subject>Erythritol</subject><subject>Eukaryotic Microbiology</subject><subject>Fibrosis</subject><subject>Fungi</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Mycology</subject><subject>Plant Sciences</subject><subject>Selective 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(HAL)</collection><jtitle>Mycopathologia (1975)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coron, Noémie</au><au>Pihet, Marc</au><au>Fréalle, Emilie</au><au>Lemeille, Yolande</au><au>Pinel, Claudine</au><au>Pelloux, Hervé</au><au>Gargala, Gilles</au><au>Favennec, Loic</au><au>Accoceberry, Isabelle</au><au>Durand-Joly, Isabelle</au><au>Dalle, Frédéric</au><au>Huet, Frédéric</au><au>Fanton, Annlyse</au><au>Boldron, Amale</au><au>Loeuille, Guy-André</au><au>Domblides, Philippe</au><au>Coltey, Bérengère</au><au>Pin, Isabelle</au><au>Llerena, Catherine</au><au>Troussier, Françoise</au><au>Person, Christine</au><au>Marguet, Christophe</au><au>Wizla, Nathalie</au><au>Thumerelle, Caroline</au><au>Turck, Dominique</au><au>Bui, Stéphanie</au><au>Fayon, Michael</au><au>Duhamel, Alain</au><au>Prévotat, Anne</au><au>Wallaert, Benoit</au><au>Leroy, Sylvie</au><au>Bouchara, Jean-Philippe</au><au>Delhaes, Laurence</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toward the Standardization of Mycological Examination of Sputum Samples in Cystic Fibrosis: Results from a French Multicenter Prospective Study</atitle><jtitle>Mycopathologia (1975)</jtitle><stitle>Mycopathologia</stitle><addtitle>Mycopathologia</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>183</volume><issue>1</issue><spage>101</spage><epage>117</epage><pages>101-117</pages><issn>0301-486X</issn><eissn>1573-0832</eissn><abstract>Fungal respiratory colonization of cystic fibrosis (CF) patients emerges as a new concern; however, the heterogeneity of mycological protocols limits investigations. We first aimed at setting up an efficient standardized protocol for mycological analysis of CF sputa that was assessed during a prospective, multicenter study: “MucoFong” program (PHRC-06/1902). Sputa from 243 CF patients from seven centers in France were collected over a 15-month period and submitted to a standardized protocol based on 6 semi-selective media. After mucolytic pretreatment, sputa were plated in parallel on cycloheximide-enriched (ACT37), erythritol-enriched (ERY37), benomyl dichloran–rose bengal (BENO37) and chromogenic (CAN37) media incubated at 37 °C and on Sabouraud–chloramphenicol (SAB27) and erythritol-enriched (ERY27) media incubated at 20–27 °C. Each plate was checked twice a week during 3 weeks. Fungi were conventionally identified; time for detection of fungal growth was noted for each species. Fungal prevalences and media performances were assessed; an optimal combination of media was determined using the Chi-squared automatic interaction detector method. At least one fungal species was isolated from 81% of sputa.
Candida albicans
was the most prevalent species (58.8%), followed by
Aspergillus fumigatus
(35.4%). Cultivation on CAN37, SAB27, ACT37 and ERY27 during 16 days provided an optimal combination, detecting
C. albicans
,
A. fumigatus
,
Scedosporium apiospermum
complex and
Exophiala
spp. with sensitivities of 96.5, 98.8, 100 and 100%. Combination of these four culture media is recommended to ensure the growth of key fungal pathogens in CF respiratory specimens. The use of such consensual protocol is of major interest for merging results from future epidemiological studies.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>28748285</pmid><doi>10.1007/s11046-017-0173-1</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0002-7529-6999</orcidid><orcidid>https://orcid.org/0000-0003-3129-979X</orcidid><orcidid>https://orcid.org/0000-0002-3365-2035</orcidid><orcidid>https://orcid.org/0000-0001-8720-5823</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-486X |
| ispartof | Mycopathologia (1975), 2018-02, Vol.183 (1), p.101-117 |
| issn | 0301-486X 1573-0832 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_01618715v1 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Analysis Antibacterial agents Benomyl Biomedical and Life Sciences Candida albicans Chloramphenicol Colonization Culture media Cycloheximide Cystic fibrosis Enrichment Epidemiology Erythritol Eukaryotic Microbiology Fibrosis Fungi Life Sciences Medical Microbiology Microbial Ecology Microbiology Microbiology and Parasitology Mycology Plant Sciences Selective media Species Sputum Standardization |
| title | Toward the Standardization of Mycological Examination of Sputum Samples in Cystic Fibrosis: Results from a French Multicenter Prospective Study |
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